OPENSEQ.org
PRMT5 292-637

ID: 1516272175 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 346 (334)
Sequences: 825 (555)
Seq/Len: 2.470
Nf(neff/√len): 30.4

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 2.470).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
221_L255_T3.2691.00
183_P222_S3.1441.00
166_L170_G2.8031.00
75_A101_E2.3621.00
183_P249_F2.1161.00
50_I84_S2.0420.99
139_D167_K2.0020.99
144_E288_W1.9810.99
144_E153_E1.9640.99
56_D269_Y1.9250.99
182_A220_Q1.9160.99
96_K123_T1.9140.99
150_A177_Y1.9010.99
161_G164_H1.8960.99
82_N113_W1.8730.99
215_L261_A1.8640.99
181_L260_F1.8610.99
147_G152_N1.8260.99
275_S278_P1.8060.98
34_E38_K1.7750.98
259_G292_L1.7430.98
143_S147_G1.7080.98
254_N302_R1.7050.98
153_E288_W1.6810.97
70_L95_I1.6780.97
266_T274_L1.6350.97
326_V332_S1.6300.97
98_Y136_E1.5400.95
72_V77_R1.5330.95
75_A99_A1.5220.94
254_N303_E1.5190.94
98_Y125_V1.5040.94
9_F12_G1.5040.94
221_L257_L1.5000.94
162_A165_F1.4820.93
262_G293_F1.4590.93
264_F268_L1.4460.92
186_S189_L1.4400.92
129_M161_G1.4240.91
151_D157_E1.4150.91
260_F309_V1.4090.91
337_P341_S1.4050.91
106_A124_V1.4020.90
98_Y138_A1.3940.90
10_A14_E1.3930.90
5_A10_A1.3870.90
5_A8_L1.3760.89
333_A341_S1.3680.89
149_F152_N1.3590.89
49_A173_I1.3490.88
264_F288_W1.3440.88
151_D156_P1.3400.88
50_I54_L1.3350.87
72_V287_S1.3310.87
250_P306_T1.3220.87
15_D18_Q1.3190.87
11_K14_E1.3150.86
150_A156_P1.2950.85
36_F42_K1.2940.85
73_L141_I1.2900.85
140_I171_V1.2840.84
248_E308_C1.2810.84
149_F293_F1.2740.84
183_P261_A1.2730.84
11_K19_S1.2720.84
33_Y36_F1.2710.84
162_A166_L1.2710.84
9_F16_Y1.2700.84
72_V81_V1.2700.84
107_V124_V1.2660.83
248_E306_T1.2610.83
56_D270_Q1.2610.83
258_H298_P1.2610.83
255_T301_V1.2550.83
146_L155_S1.2390.81
82_N100_V1.2320.81
99_A124_V1.2300.81
69_V98_Y1.2240.80
13_Y16_Y1.2190.80
88_A207_F1.2150.80
225_Q249_F1.2090.79
187_S191_N1.2080.79
11_K16_Y1.2070.79
13_Y19_S1.2010.79
52_K271_D1.1930.78
74_G144_E1.1910.78
178_T265_E1.1900.78
70_L94_R1.1880.78
36_F144_E1.1810.77
173_I268_L1.1680.76
332_S335_H1.1680.76
151_D230_F1.1600.75
101_E106_A1.1530.75
190_Y258_H1.1440.74
29_E34_E1.1410.73
71_M138_A1.1400.73
177_Y232_H1.1360.73
53_C171_V1.1350.73
138_A165_F1.1330.73
154_L157_E1.1310.73
6_Y11_K1.1270.72
57_R68_Q1.1100.70
299_I326_V1.1070.70
36_F43_Y1.1050.70
36_F77_R1.1020.70
298_P301_V1.0980.69
137_K168_D1.0960.69
155_S232_H1.0940.69
333_A337_P1.0910.69
149_F262_G1.0910.69
301_V304_G1.0910.69
12_G16_Y1.0850.68
162_A170_G1.0840.68
264_F278_P1.0760.67
138_A170_G1.0750.67
44_S48_Q1.0740.67
7_E10_A1.0740.67
125_V299_I1.0660.66
6_Y10_A1.0640.66
278_P290_P1.0620.66
32_T147_G1.0580.65
94_R97_L1.0570.65
147_G266_T1.0560.65
43_Y152_N1.0540.65
51_Y90_Q1.0530.65
255_T272_I1.0510.64
79_P86_R1.0490.64
178_T226_P1.0490.64
14_E83_A1.0490.64
10_A13_Y1.0470.64
8_L11_K1.0450.64
36_F72_V1.0440.64
5_A9_F1.0430.64
268_L294_P1.0420.63
295_I299_I1.0420.63
336_N341_S1.0420.63
53_C140_I1.0410.63
33_Y37_E1.0380.63
42_K144_E1.0360.63
75_A84_S1.0340.63
106_A212_V1.0320.62
275_S290_P1.0310.62
123_T311_F1.0290.62
114_Q120_S1.0270.62
27_N105_N1.0230.61
103_N145_L1.0220.61
125_V132_W1.0220.61
139_D143_S1.0180.61
36_F129_M1.0180.61
315_S332_S1.0170.61
129_M145_L1.0170.61
102_K157_E1.0120.60
155_S212_V1.0100.60
107_V110_L1.0100.60
251_V307_I1.0060.59
32_T152_N1.0050.59
136_E167_K1.0050.59
96_K125_V1.0040.59
6_Y9_F1.0030.59
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3ua3A20.94511000.306Contact Map
4gqbA10.99711000.357Contact Map
2fytA10.85551000.495Contact Map
4lwoB40.82661000.503Contact Map
3x0dA10.90171000.51Contact Map
4c4aA10.86421000.511Contact Map
1g6q160.89021000.512Contact Map
3q7eA10.86711000.514Contact Map
4m37A10.88151000.516Contact Map
4ikpA40.88731000.524Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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