OPENSEQ.org
PRMT5 1-300

ID: 1515426618 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 300 (291)
Sequences: 316 (222.7)
Seq/Len: 1.086
Nf(neff/√len): 13.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.086).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
84_G119_L4.0961.00
226_I257_L3.1451.00
253_L257_L2.1130.97
89_W103_S1.9710.96
73_L78_W1.8840.94
239_N251_Q1.8610.94
212_L250_H1.8600.94
229_A257_L1.8000.92
90_I103_S1.7130.89
89_W110_E1.6700.88
103_S110_E1.6220.86
196_C202_I1.6130.85
47_H52_R1.6080.85
127_N134_L1.5660.83
90_I110_E1.5660.83
78_W82_I1.5580.82
83_V121_A1.5030.79
221_W224_E1.5020.79
121_A152_W1.4780.77
142_I202_I1.4710.77
117_L228_A1.4610.76
103_S107_M1.4540.76
186_W189_W1.4510.76
286_Y290_N1.4270.74
265_I284_L1.4230.74
213_P250_H1.4030.72
237_L247_S1.3950.71
73_L80_T1.3920.71
107_M111_L1.3800.70
89_W106_A1.3790.70
125_P212_L1.3710.70
208_I295_N1.3590.69
70_D157_L1.3380.67
272_H280_Y1.3170.65
103_S298_E1.3100.64
126_L134_L1.3070.64
125_P154_R1.3070.64
103_S106_A1.3030.64
90_I298_E1.3000.64
193_R198_Y1.3000.64
152_W263_Q1.3000.64
189_W193_R1.2970.63
186_W193_R1.2890.63
65_P68_R1.2850.62
239_N244_P1.2840.62
19_D113_F1.2820.62
248_K251_Q1.2810.62
190_H193_R1.2680.61
173_T176_T1.2550.60
82_I296_A1.2460.59
99_I198_Y1.2440.59
133_N136_R1.2330.58
184_K220_R1.2300.57
95_K266_I1.2260.57
92_P123_L1.2250.57
70_D73_L1.2240.57
118_G258_L1.2190.56
19_D42_C1.2140.56
203_A227_K1.2110.55
173_T177_E1.2020.55
153_M185_T1.1940.54
91_R106_A1.1910.54
113_F198_Y1.1880.53
48_P70_D1.1860.53
189_W225_P1.1840.53
239_N283_Y1.1800.53
114_G239_N1.1780.52
194_T198_Y1.1750.52
127_N202_I1.1690.52
233_T268_G1.1600.51
42_C126_L1.1590.51
83_V120_P1.1560.50
10_G13_R1.1530.50
42_C268_G1.1480.50
45_V264_F1.1450.49
221_W225_P1.1370.48
239_N245_V1.1370.48
45_V235_I1.1340.48
251_Q283_Y1.1300.48
39_D281_L1.1250.47
44_P136_R1.1240.47
185_T217_V1.1240.47
41_L276_E1.1220.47
107_M153_M1.1160.46
266_I284_L1.1150.46
118_G209_G1.1110.46
244_P251_Q1.1110.46
21_N268_G1.1070.46
247_S251_Q1.1030.45
239_N242_G1.1010.45
207_E232_P1.0930.44
193_R287_L1.0920.44
95_K204_V1.0890.44
150_M300_F1.0880.44
184_K241_K1.0880.44
173_T178_E1.0810.43
125_P250_H1.0800.43
49_R268_G1.0790.43
118_G157_L1.0790.43
241_K245_V1.0730.42
66_Q133_N1.0720.42
190_H194_T1.0670.42
287_L300_F1.0670.42
20_L105_A1.0630.42
276_E279_S1.0560.41
25_E31_G1.0550.41
244_P248_K1.0550.41
128_Q253_L1.0510.40
175_H178_E1.0510.40
26_I68_R1.0490.40
59_A173_T1.0470.40
37_G194_T1.0460.40
26_I65_P1.0440.40
245_V251_Q1.0410.40
63_P187_M1.0400.39
33_V38_F1.0400.39
50_F70_D1.0370.39
239_N248_K1.0360.39
48_P287_L1.0360.39
193_R196_C1.0330.39
235_I289_Q1.0320.39
151_F265_I1.0300.39
64_G67_T1.0290.38
203_A243_F1.0280.38
71_L133_N1.0280.38
73_L94_S1.0260.38
257_L289_Q1.0260.38
166_D175_H1.0260.38
197_D252_R1.0240.38
75_G209_G1.0230.38
113_F154_R1.0230.38
260_L291_R1.0220.38
283_Y287_L1.0200.38
69_S178_E1.0180.37
70_D91_R1.0160.37
89_W259_K1.0120.37
146_H196_C1.0120.37
245_V292_P1.0080.37
24_P174_T1.0080.37
127_N204_V1.0070.36
131_N223_G1.0060.36
187_M222_L1.0040.36
16_S284_L1.0020.36
97_E122_F1.0010.36
186_W205_A1.0010.36
72_L243_F1.0000.36
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4gqbA10.961000.223Contact Map
3ua3A20.89671000.262Contact Map
4hnoA10.846772.70.957Contact Map
1qtwA10.843341.50.964Contact Map
3tc3A20.796732.60.966Contact Map
3aalA10.786731.60.966Contact Map
2qw5A20.773323.80.968Contact Map
3aamA10.813321.50.969Contact Map
3wqoA20.8118.70.97Contact Map
3kwsA20.796717.30.97Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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