| hsp90a |
| ID: | 1506723228 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 737 (667) | ||
| Sequences: | 2537 (1263.3) | ||
| Seq/Len: | 3.804 | ||
| Nf(neff/√len): | 48.9 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.804).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 156_I | 192_I | 4.786 | 1.00 |
| 501_G | 507_V | 4.609 | 1.00 |
| 315_G | 327_D | 4.476 | 1.00 |
| 97_V | 190_K | 4.340 | 1.00 |
| 112_L | 155_V | 4.306 | 1.00 |
| 488_R | 522_E | 3.771 | 1.00 |
| 580_M | 668_V | 3.663 | 1.00 |
| 201_E | 207_R | 3.647 | 1.00 |
| 88_N | 91_D | 3.528 | 1.00 |
| 92_R | 200_T | 3.497 | 1.00 |
| 152_K | 168_E | 3.483 | 1.00 |
| 435_D | 438_N | 3.414 | 1.00 |
| 474_A | 520_G | 3.334 | 1.00 |
| 89_K | 204_E | 3.170 | 1.00 |
| 85_L | 212_V | 3.167 | 1.00 |
| 673_E | 692_R | 3.152 | 1.00 |
| 154_T | 168_E | 3.135 | 1.00 |
| 571_T | 574_E | 3.110 | 1.00 |
| 581_K | 590_K | 3.043 | 1.00 |
| 465_S | 538_L | 3.035 | 1.00 |
| 483_K | 541_F | 3.034 | 1.00 |
| 574_E | 578_K | 3.033 | 1.00 |
| 473_S | 522_E | 2.994 | 1.00 |
| 104_T | 159_H | 2.957 | 1.00 |
| 107_D | 159_H | 2.855 | 1.00 |
| 580_M | 641_I | 2.838 | 1.00 |
| 484_D | 487_T | 2.830 | 1.00 |
| 156_I | 166_A | 2.828 | 1.00 |
| 504_K | 508_A | 2.813 | 1.00 |
| 151_E | 194_H | 2.811 | 1.00 |
| 309_I | 313_E | 2.774 | 1.00 |
| 108_L | 157_T | 2.758 | 1.00 |
| 319_K | 327_D | 2.706 | 1.00 |
| 209_K | 293_E | 2.606 | 1.00 |
| 82_H | 224_T | 2.605 | 1.00 |
| 106_A | 110_N | 2.583 | 1.00 |
| 473_S | 525_Y | 2.565 | 1.00 |
| 205_E | 209_K | 2.560 | 1.00 |
| 61_L | 81_L | 2.540 | 1.00 |
| 472_T | 524_I | 2.517 | 1.00 |
| 54_I | 212_V | 2.492 | 1.00 |
| 154_T | 194_H | 2.483 | 1.00 |
| 207_R | 210_E | 2.482 | 1.00 |
| 540_E | 543_G | 2.461 | 1.00 |
| 166_A | 178_R | 2.432 | 1.00 |
| 67_E | 159_H | 2.349 | 1.00 |
| 156_I | 190_K | 2.348 | 1.00 |
| 588_V | 591_V | 2.343 | 1.00 |
| 122_A | 125_E | 2.324 | 1.00 |
| 23_E | 105_K | 2.305 | 1.00 |
| 311_N | 331_V | 2.293 | 1.00 |
| 394_V | 421_L | 2.290 | 1.00 |
| 84_N | 292_Q | 2.288 | 1.00 |
| 233_K | 287_E | 2.232 | 1.00 |
| 666_D | 695_R | 2.209 | 1.00 |
| 228_E | 288_K | 2.198 | 1.00 |
| 212_V | 295_L | 2.194 | 1.00 |
| 231_R | 289_Y | 2.187 | 1.00 |
| 578_K | 582_D | 2.173 | 1.00 |
| 567_E | 571_T | 2.150 | 1.00 |
| 86_I | 97_V | 2.109 | 1.00 |
| 350_R | 441_K | 2.084 | 1.00 |
| 152_K | 194_H | 2.082 | 1.00 |
| 516_L | 549_V | 2.060 | 1.00 |
| 226_F | 292_Q | 1.995 | 1.00 |
| 164_Q | 179_T | 1.987 | 1.00 |
| 310_T | 313_E | 1.973 | 1.00 |
| 535_V | 546_L | 1.966 | 1.00 |
| 47_E | 207_R | 1.942 | 1.00 |
| 120_T | 135_M | 1.932 | 1.00 |
| 150_A | 193_L | 1.926 | 1.00 |
| 163_E | 179_T | 1.922 | 1.00 |
| 589_E | 607_S | 1.921 | 1.00 |
| 351_R | 445_Q | 1.920 | 1.00 |
| 441_K | 444_E | 1.881 | 1.00 |
| 301_I | 309_I | 1.854 | 1.00 |
| 146_A | 153_V | 1.854 | 1.00 |
| 47_E | 202_Y | 1.852 | 1.00 |
| 326_E | 351_R | 1.846 | 1.00 |
| 61_L | 221_Y | 1.843 | 1.00 |
| 156_I | 180_D | 1.818 | 0.99 |
| 602_C | 671_L | 1.817 | 0.99 |
| 117_K | 122_A | 1.816 | 0.99 |
| 152_K | 170_S | 1.796 | 0.99 |
| 87_P | 208_I | 1.779 | 0.99 |
| 482_L | 542_E | 1.755 | 0.99 |
| 311_N | 315_G | 1.748 | 0.99 |
| 390_I | 425_C | 1.747 | 0.99 |
| 93_T | 192_I | 1.735 | 0.99 |
| 587_K | 669_I | 1.735 | 0.99 |
| 473_S | 488_R | 1.728 | 0.99 |
| 490_K | 520_G | 1.727 | 0.99 |
| 466_E | 483_K | 1.724 | 0.99 |
| 224_T | 294_E | 1.723 | 0.99 |
| 154_T | 166_A | 1.716 | 0.99 |
| 230_E | 286_K | 1.705 | 0.99 |
| 508_A | 525_Y | 1.696 | 0.99 |
| 230_E | 288_K | 1.693 | 0.99 |
| 568_E | 572_K | 1.680 | 0.99 |
| 513_V | 549_V | 1.677 | 0.99 |
| 33_Q | 121_K | 1.673 | 0.99 |
| 46_K | 199_Q | 1.655 | 0.99 |
| 596_R | 605_V | 1.653 | 0.99 |
| 84_N | 97_V | 1.625 | 0.99 |
| 315_G | 331_V | 1.624 | 0.99 |
| 96_I | 191_V | 1.621 | 0.99 |
| 582_D | 585_E | 1.610 | 0.98 |
| 157_T | 175_F | 1.610 | 0.98 |
| 108_L | 112_L | 1.610 | 0.98 |
| 466_E | 542_E | 1.601 | 0.98 |
| 470_Y | 526_M | 1.584 | 0.98 |
| 314_Y | 347_F | 1.584 | 0.98 |
| 84_N | 224_T | 1.579 | 0.98 |
| 567_E | 570_K | 1.576 | 0.98 |
| 154_T | 192_I | 1.575 | 0.98 |
| 614_N | 617_R | 1.564 | 0.98 |
| 168_E | 176_T | 1.551 | 0.98 |
| 122_A | 126_A | 1.550 | 0.98 |
| 685_D | 688_T | 1.539 | 0.98 |
| 304_R | 313_E | 1.528 | 0.98 |
| 232_D | 284_K | 1.526 | 0.98 |
| 568_E | 571_T | 1.514 | 0.97 |
| 224_T | 292_Q | 1.493 | 0.97 |
| 39_I | 48_I | 1.490 | 0.97 |
| 616_E | 637_K | 1.487 | 0.97 |
| 583_I | 668_V | 1.485 | 0.97 |
| 315_G | 328_H | 1.480 | 0.97 |
| 579_I | 664_V | 1.479 | 0.97 |
| 50_L | 96_I | 1.473 | 0.97 |
| 226_F | 290_I | 1.469 | 0.97 |
| 474_A | 490_K | 1.467 | 0.97 |
| 228_E | 290_I | 1.467 | 0.97 |
| 89_K | 203_L | 1.466 | 0.97 |
| 229_K | 289_Y | 1.457 | 0.97 |
| 22_V | 178_R | 1.456 | 0.97 |
| 53_L | 193_L | 1.448 | 0.96 |
| 110_N | 122_A | 1.445 | 0.96 |
| 650_T | 654_K | 1.444 | 0.96 |
| 466_E | 541_F | 1.436 | 0.96 |
| 478_E | 482_L | 1.434 | 0.96 |
| 146_A | 193_L | 1.432 | 0.96 |
| 495_H | 547_V | 1.429 | 0.96 |
| 327_D | 350_R | 1.417 | 0.96 |
| 400_P | 411_S | 1.415 | 0.96 |
| 581_K | 585_E | 1.410 | 0.96 |
| 387_L | 426_L | 1.402 | 0.96 |
| 105_K | 109_I | 1.401 | 0.96 |
| 352_A | 445_Q | 1.396 | 0.95 |
| 514_E | 600_S | 1.388 | 0.95 |
| 478_E | 481_S | 1.376 | 0.95 |
| 321_L | 325_W | 1.375 | 0.95 |
| 95_T | 190_K | 1.369 | 0.95 |
| 229_K | 293_E | 1.367 | 0.95 |
| 494_K | 558_E | 1.357 | 0.94 |
| 316_E | 319_K | 1.357 | 0.94 |
| 85_L | 295_L | 1.352 | 0.94 |
| 643_P | 646_S | 1.343 | 0.94 |
| 85_L | 208_I | 1.341 | 0.94 |
| 85_L | 94_L | 1.335 | 0.94 |
| 64_I | 160_N | 1.334 | 0.94 |
| 669_I | 692_R | 1.328 | 0.94 |
| 489_M | 544_K | 1.324 | 0.93 |
| 432_L | 480_V | 1.324 | 0.93 |
| 483_K | 542_E | 1.318 | 0.93 |
| 479_M | 482_L | 1.318 | 0.93 |
| 575_N | 578_K | 1.312 | 0.93 |
| 646_S | 649_E | 1.308 | 0.93 |
| 495_H | 545_T | 1.306 | 0.93 |
| 486_C | 542_E | 1.306 | 0.93 |
| 503_T | 506_Q | 1.305 | 0.93 |
| 469_R | 481_S | 1.303 | 0.93 |
| 508_A | 517_R | 1.295 | 0.92 |
| 312_E | 316_E | 1.291 | 0.92 |
| 49_F | 149_V | 1.281 | 0.92 |
| 64_I | 187_R | 1.281 | 0.92 |
| 641_I | 648_I | 1.280 | 0.92 |
| 209_K | 227_V | 1.280 | 0.92 |
| 437_E | 441_K | 1.273 | 0.92 |
| 363_K | 380_E | 1.267 | 0.91 |
| 42_F | 403_I | 1.265 | 0.91 |
| 589_E | 636_K | 1.261 | 0.91 |
| 67_E | 71_D | 1.252 | 0.91 |
| 388_N | 452_L | 1.249 | 0.90 |
| 209_K | 225_L | 1.248 | 0.90 |
| 540_E | 545_T | 1.245 | 0.90 |
| 666_D | 692_R | 1.245 | 0.90 |
| 81_L | 187_R | 1.244 | 0.90 |
| 208_I | 212_V | 1.243 | 0.90 |
| 314_Y | 333_H | 1.241 | 0.90 |
| 383_I | 421_L | 1.241 | 0.90 |
| 479_M | 488_R | 1.239 | 0.90 |
| 108_L | 155_V | 1.239 | 0.90 |
| 518_K | 598_V | 1.237 | 0.90 |
| 516_L | 554_L | 1.234 | 0.90 |
| 25_F | 175_F | 1.232 | 0.90 |
| 672_Y | 676_L | 1.227 | 0.89 |
| 305_N | 308_D | 1.225 | 0.89 |
| 454_I | 534_C | 1.220 | 0.89 |
| 233_K | 285_I | 1.220 | 0.89 |
| 559_D | 562_E | 1.211 | 0.89 |
| 649_E | 653_Q | 1.206 | 0.88 |
| 107_D | 110_N | 1.205 | 0.88 |
| 47_E | 214_K | 1.201 | 0.88 |
| 205_E | 227_V | 1.190 | 0.87 |
| 44_S | 407_M | 1.186 | 0.87 |
| 472_T | 488_R | 1.185 | 0.87 |
| 168_E | 178_R | 1.184 | 0.87 |
| 227_V | 293_E | 1.183 | 0.87 |
| 232_D | 286_K | 1.183 | 0.87 |
| 691_N | 695_R | 1.178 | 0.87 |
| 232_D | 235_V | 1.177 | 0.87 |
| 472_T | 480_V | 1.177 | 0.87 |
| 648_I | 671_L | 1.176 | 0.87 |
| 665_K | 669_I | 1.174 | 0.86 |
| 587_K | 684_E | 1.173 | 0.86 |
| 569_K | 644_D | 1.166 | 0.86 |
| 690_A | 694_Y | 1.166 | 0.86 |
| 334_F | 344_A | 1.159 | 0.85 |
| 450_I | 464_L | 1.158 | 0.85 |
| 233_K | 282_K | 1.154 | 0.85 |
| 596_R | 640_E | 1.150 | 0.85 |
| 563_K | 567_E | 1.148 | 0.85 |
| 439_Y | 467_L | 1.142 | 0.84 |
| 24_T | 176_T | 1.142 | 0.84 |
| 231_R | 287_E | 1.141 | 0.84 |
| 312_E | 315_G | 1.139 | 0.84 |
| 164_Q | 180_D | 1.136 | 0.84 |
| 235_V | 282_K | 1.133 | 0.84 |
| 570_K | 574_E | 1.129 | 0.83 |
| 309_I | 333_H | 1.128 | 0.83 |
| 532_E | 612_T | 1.123 | 0.83 |
| 61_L | 99_T | 1.119 | 0.83 |
| 237_D | 240_A | 1.118 | 0.83 |
| 579_I | 583_I | 1.118 | 0.83 |
| 331_V | 347_F | 1.118 | 0.83 |
| 104_T | 107_D | 1.117 | 0.82 |
| 572_K | 652_R | 1.116 | 0.82 |
| 662_K | 665_K | 1.114 | 0.82 |
| 479_M | 522_E | 1.110 | 0.82 |
| 689_H | 693_I | 1.108 | 0.82 |
| 592_V | 638_H | 1.105 | 0.82 |
| 670_L | 689_H | 1.103 | 0.81 |
| 128_Q | 131_A | 1.103 | 0.81 |
| 105_K | 179_T | 1.101 | 0.81 |
| 577_C | 593_V | 1.096 | 0.81 |
| 110_N | 117_K | 1.093 | 0.81 |
| 121_K | 125_E | 1.086 | 0.80 |
| 494_K | 545_T | 1.082 | 0.80 |
| 306_P | 309_I | 1.081 | 0.80 |
| 112_L | 177_V | 1.078 | 0.79 |
| 103_M | 108_L | 1.078 | 0.79 |
| 439_Y | 443_Y | 1.077 | 0.79 |
| 334_F | 424_K | 1.077 | 0.79 |
| 53_L | 146_A | 1.076 | 0.79 |
| 69_L | 72_P | 1.076 | 0.79 |
| 463_K | 466_E | 1.075 | 0.79 |
| 87_P | 94_L | 1.075 | 0.79 |
| 326_E | 350_R | 1.074 | 0.79 |
| 462_K | 542_E | 1.071 | 0.79 |
| 669_I | 673_E | 1.070 | 0.79 |
| 86_I | 94_L | 1.063 | 0.78 |
| 96_I | 193_L | 1.059 | 0.78 |
| 615_M | 619_M | 1.059 | 0.78 |
| 457_D | 460_N | 1.059 | 0.78 |
| 25_F | 105_K | 1.059 | 0.78 |
| 454_I | 468_L | 1.057 | 0.77 |
| 78_G | 187_R | 1.048 | 0.77 |
| 513_V | 523_V | 1.045 | 0.76 |
| 586_K | 636_K | 1.044 | 0.76 |
| 518_K | 599_T | 1.042 | 0.76 |
| 212_V | 577_C | 1.042 | 0.76 |
| 204_E | 207_R | 1.040 | 0.76 |
| 573_F | 652_R | 1.040 | 0.76 |
| 348_V | 390_I | 1.040 | 0.76 |
| 436_K | 484_D | 1.033 | 0.75 |
| 84_N | 226_F | 1.031 | 0.75 |
| 329_L | 438_N | 1.029 | 0.75 |
| 232_D | 283_K | 1.027 | 0.75 |
| 86_I | 95_T | 1.026 | 0.74 |
| 236_S | 281_K | 1.025 | 0.74 |
| 480_V | 524_I | 1.022 | 0.74 |
| 68_S | 74_K | 1.022 | 0.74 |
| 649_E | 652_R | 1.022 | 0.74 |
| 337_E | 341_E | 1.020 | 0.74 |
| 447_S | 451_K | 1.019 | 0.74 |
| 235_V | 284_K | 1.018 | 0.74 |
| 471_Y | 504_K | 1.017 | 0.73 |
| 371_R | 396_S | 1.012 | 0.73 |
| 46_K | 196_K | 1.012 | 0.73 |
| 486_C | 489_M | 1.011 | 0.73 |
| 114_T | 117_K | 1.010 | 0.73 |
| 303_T | 343_R | 1.010 | 0.73 |
| 111_N | 117_K | 1.009 | 0.73 |
| 229_K | 291_D | 1.008 | 0.73 |
| 166_A | 192_I | 1.004 | 0.72 |
| 489_M | 493_Q | 1.003 | 0.72 |
| 483_K | 486_C | 1.003 | 0.72 |
| 107_D | 111_N | 1.002 | 0.72 |
| 374_F | 383_I | 1.001 | 0.72 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2cg9A | 2 | 0.8358 | 100 | 0.205 | Contact Map |
| 2o1uA | 2 | 0.7775 | 100 | 0.257 | Contact Map |
| 2ioqA | 2 | 0.7897 | 100 | 0.276 | Contact Map |
| 4ivgA | 1 | 0.6323 | 100 | 0.303 | Contact Map |
| 4ipeA | 2 | 0.8114 | 100 | 0.307 | Contact Map |
| 1y4sA | 2 | 0.6364 | 100 | 0.334 | Contact Map |
| 3q6mA | 3 | 0.5088 | 100 | 0.46 | Contact Map |
| 3hjcA | 1 | 0.5251 | 100 | 0.475 | Contact Map |
| 2cgeA | 3 | 0.5482 | 100 | 0.484 | Contact Map |
| 1hk7A | 2 | 0.3474 | 100 | 0.609 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)