| FunGlcSUN |
| ID: | 1502722356 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 276 (275) | ||
| Sequences: | 365 (243.6) | ||
| Seq/Len: | 1.327 | ||
| Nf(neff/√len): | 14.7 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.327).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 25_P | 99_Y | 4.244 | 1.00 |
| 18_K | 108_D | 2.940 | 1.00 |
| 127_D | 146_Y | 2.901 | 1.00 |
| 96_K | 101_Y | 2.489 | 1.00 |
| 80_P | 90_I | 2.453 | 1.00 |
| 183_S | 186_D | 2.364 | 0.99 |
| 130_I | 274_L | 2.281 | 0.99 |
| 129_A | 183_S | 2.184 | 0.99 |
| 213_V | 264_S | 2.180 | 0.99 |
| 77_T | 112_E | 2.063 | 0.98 |
| 244_N | 264_S | 2.007 | 0.97 |
| 122_S | 218_L | 1.988 | 0.97 |
| 182_I | 187_A | 1.899 | 0.96 |
| 48_G | 138_T | 1.889 | 0.96 |
| 18_K | 109_Y | 1.870 | 0.96 |
| 38_I | 47_S | 1.840 | 0.95 |
| 132_R | 175_Y | 1.701 | 0.92 |
| 119_Y | 152_S | 1.683 | 0.91 |
| 115_V | 209_S | 1.672 | 0.91 |
| 34_G | 71_Q | 1.658 | 0.91 |
| 74_M | 113_W | 1.637 | 0.90 |
| 9_L | 15_P | 1.629 | 0.90 |
| 177_V | 232_V | 1.613 | 0.89 |
| 203_L | 232_V | 1.589 | 0.88 |
| 177_V | 230_F | 1.584 | 0.88 |
| 155_L | 205_F | 1.583 | 0.88 |
| 235_V | 273_V | 1.555 | 0.86 |
| 40_W | 184_V | 1.532 | 0.85 |
| 146_Y | 184_V | 1.526 | 0.85 |
| 83_Q | 87_G | 1.478 | 0.82 |
| 9_L | 12_F | 1.473 | 0.82 |
| 7_G | 12_F | 1.468 | 0.82 |
| 5_I | 36_I | 1.467 | 0.82 |
| 233_K | 248_I | 1.452 | 0.81 |
| 161_D | 170_K | 1.441 | 0.80 |
| 180_A | 229_N | 1.417 | 0.79 |
| 5_I | 9_L | 1.415 | 0.78 |
| 205_F | 272_F | 1.386 | 0.76 |
| 183_S | 187_A | 1.384 | 0.76 |
| 38_I | 41_L | 1.373 | 0.75 |
| 33_Q | 71_Q | 1.370 | 0.75 |
| 34_G | 74_M | 1.349 | 0.74 |
| 5_I | 12_F | 1.344 | 0.73 |
| 215_Y | 229_N | 1.339 | 0.73 |
| 203_L | 218_L | 1.336 | 0.73 |
| 7_G | 15_P | 1.334 | 0.72 |
| 231_N | 275_Y | 1.332 | 0.72 |
| 117_A | 214_A | 1.324 | 0.72 |
| 120_V | 147_V | 1.311 | 0.71 |
| 75_S | 156_T | 1.304 | 0.70 |
| 121_V | 271_K | 1.303 | 0.70 |
| 179_N | 229_N | 1.301 | 0.70 |
| 29_F | 46_W | 1.297 | 0.69 |
| 116_D | 214_A | 1.294 | 0.69 |
| 5_I | 8_D | 1.291 | 0.69 |
| 124_L | 274_L | 1.288 | 0.69 |
| 49_V | 64_S | 1.281 | 0.68 |
| 50_E | 88_R | 1.278 | 0.68 |
| 231_N | 250_E | 1.263 | 0.66 |
| 8_D | 15_P | 1.261 | 0.66 |
| 233_K | 273_V | 1.258 | 0.66 |
| 12_F | 15_P | 1.257 | 0.66 |
| 25_P | 28_Q | 1.249 | 0.65 |
| 120_V | 155_L | 1.247 | 0.65 |
| 204_N | 228_L | 1.224 | 0.63 |
| 203_L | 228_L | 1.222 | 0.63 |
| 246_E | 256_G | 1.206 | 0.61 |
| 8_D | 12_F | 1.198 | 0.60 |
| 209_S | 214_A | 1.193 | 0.60 |
| 56_T | 138_T | 1.192 | 0.60 |
| 61_K | 64_S | 1.185 | 0.59 |
| 218_L | 232_V | 1.180 | 0.59 |
| 34_G | 68_Y | 1.174 | 0.58 |
| 15_P | 71_Q | 1.170 | 0.58 |
| 265_V | 270_A | 1.166 | 0.57 |
| 62_E | 94_L | 1.163 | 0.57 |
| 73_G | 78_Q | 1.163 | 0.57 |
| 9_L | 113_W | 1.157 | 0.56 |
| 10_A | 13_S | 1.154 | 0.56 |
| 89_S | 164_Y | 1.152 | 0.56 |
| 53_D | 257_G | 1.142 | 0.55 |
| 120_V | 218_L | 1.138 | 0.55 |
| 50_E | 138_T | 1.135 | 0.54 |
| 21_D | 101_Y | 1.134 | 0.54 |
| 129_A | 184_V | 1.132 | 0.54 |
| 9_L | 71_Q | 1.129 | 0.54 |
| 132_R | 163_Y | 1.123 | 0.53 |
| 5_I | 15_P | 1.123 | 0.53 |
| 15_P | 113_W | 1.121 | 0.53 |
| 205_F | 274_L | 1.118 | 0.53 |
| 41_L | 130_I | 1.113 | 0.52 |
| 102_R | 105_T | 1.110 | 0.52 |
| 6_Y | 153_L | 1.109 | 0.52 |
| 123_E | 151_D | 1.108 | 0.52 |
| 177_V | 205_F | 1.106 | 0.51 |
| 14_G | 17_E | 1.104 | 0.51 |
| 147_V | 192_S | 1.098 | 0.51 |
| 29_F | 34_G | 1.096 | 0.50 |
| 2_N | 13_S | 1.094 | 0.50 |
| 147_V | 153_L | 1.093 | 0.50 |
| 35_V | 77_T | 1.091 | 0.50 |
| 13_S | 165_T | 1.087 | 0.49 |
| 50_E | 228_L | 1.087 | 0.49 |
| 35_V | 71_Q | 1.084 | 0.49 |
| 126_N | 253_S | 1.079 | 0.49 |
| 58_G | 138_T | 1.079 | 0.49 |
| 8_D | 71_Q | 1.076 | 0.48 |
| 85_S | 244_N | 1.071 | 0.48 |
| 254_F | 260_G | 1.071 | 0.48 |
| 138_T | 142_V | 1.071 | 0.48 |
| 75_S | 132_R | 1.070 | 0.48 |
| 19_F | 22_G | 1.066 | 0.47 |
| 251_N | 258_S | 1.060 | 0.47 |
| 75_S | 158_V | 1.059 | 0.47 |
| 60_C | 66_C | 1.054 | 0.46 |
| 237_A | 269_K | 1.050 | 0.46 |
| 9_L | 109_Y | 1.049 | 0.46 |
| 72_P | 121_V | 1.047 | 0.45 |
| 59_S | 120_V | 1.047 | 0.45 |
| 129_A | 187_A | 1.046 | 0.45 |
| 98_G | 268_G | 1.045 | 0.45 |
| 233_K | 275_Y | 1.039 | 0.45 |
| 47_S | 138_T | 1.036 | 0.44 |
| 40_W | 46_W | 1.036 | 0.44 |
| 13_S | 17_E | 1.035 | 0.44 |
| 26_C | 98_G | 1.033 | 0.44 |
| 29_F | 74_M | 1.029 | 0.44 |
| 129_A | 146_Y | 1.027 | 0.43 |
| 27_G | 46_W | 1.026 | 0.43 |
| 24_I | 99_Y | 1.023 | 0.43 |
| 63_G | 187_A | 1.022 | 0.43 |
| 148_Q | 151_D | 1.015 | 0.42 |
| 6_Y | 14_G | 1.008 | 0.42 |
| 196_G | 230_F | 1.004 | 0.41 |
| 6_Y | 11_D | 1.002 | 0.41 |
| 156_T | 173_A | 1.001 | 0.41 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2ehfA | 1 | 0.25 | 13.2 | 0.976 | Contact Map |
| 4jhsA | 1 | 0.3986 | 4.5 | 0.98 | Contact Map |
| 1x7aL | 1 | 0.3152 | 3 | 0.982 | Contact Map |
| 2m7cA | 1 | 0.0688 | 2.4 | 0.983 | Contact Map |
| 3isyA | 1 | 0.1667 | 1.8 | 0.984 | Contact Map |
| 1qubA | 1 | 0.3768 | 1.5 | 0.985 | Contact Map |
| 2mh5A | 1 | 0.0435 | 1.5 | 0.985 | Contact Map |
| 3zmrA | 2 | 0.1812 | 1.5 | 0.985 | Contact Map |
| 1xynA | 1 | 0.3333 | 1.5 | 0.985 | Contact Map |
| 2hb0A | 2 | 0.4094 | 1.3 | 0.985 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)