| gpcr_1e-40 |
| ID: | 1501613380 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 292 (282) | ||
| Sequences: | 7252 (4014.7) | ||
| Seq/Len: | 25.716 | ||
| Nf(neff/√len): | 239.1 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 25.716).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 13_S | 55_T | 5.230 | 1.00 |
| 76_L | 80_V | 3.807 | 1.00 |
| 193_I | 197_K | 3.602 | 1.00 |
| 104_S | 109_R | 2.931 | 1.00 |
| 46_H | 128_W | 2.837 | 1.00 |
| 98_V | 127_A | 2.823 | 1.00 |
| 75_E | 79_K | 2.812 | 1.00 |
| 46_H | 95_M | 2.717 | 1.00 |
| 71_W | 74_G | 2.588 | 1.00 |
| 18_A | 22_L | 2.492 | 1.00 |
| 21_L | 45_K | 2.328 | 1.00 |
| 83_Y | 87_F | 2.296 | 1.00 |
| 98_V | 123_M | 2.181 | 1.00 |
| 239_S | 286_I | 2.147 | 1.00 |
| 197_K | 200_F | 2.081 | 1.00 |
| 196_A | 200_F | 2.026 | 1.00 |
| 244_W | 276_A | 2.018 | 1.00 |
| 24_L | 45_K | 2.008 | 1.00 |
| 104_S | 197_K | 1.931 | 1.00 |
| 87_F | 132_S | 1.927 | 1.00 |
| 64_M | 73_A | 1.921 | 1.00 |
| 42_L | 124_V | 1.906 | 1.00 |
| 200_F | 204_R | 1.830 | 1.00 |
| 190_I | 194_C | 1.775 | 1.00 |
| 267_P | 271_F | 1.760 | 1.00 |
| 75_E | 143_R | 1.753 | 1.00 |
| 43_L | 120_G | 1.749 | 1.00 |
| 101_L | 194_C | 1.715 | 1.00 |
| 232_M | 290_F | 1.707 | 1.00 |
| 83_Y | 139_L | 1.703 | 1.00 |
| 95_M | 124_V | 1.687 | 1.00 |
| 48_T | 51_N | 1.682 | 1.00 |
| 180_F | 185_I | 1.668 | 1.00 |
| 102_D | 106_A | 1.639 | 1.00 |
| 136_G | 140_Y | 1.628 | 1.00 |
| 247_Y | 251_G | 1.617 | 1.00 |
| 21_L | 52_L | 1.595 | 1.00 |
| 250_L | 268_V | 1.581 | 1.00 |
| 91_A | 131_S | 1.563 | 1.00 |
| 180_F | 252_I | 1.554 | 1.00 |
| 283_D | 287_Y | 1.545 | 1.00 |
| 36_K | 41_K | 1.536 | 1.00 |
| 123_M | 127_A | 1.535 | 1.00 |
| 188_L | 241_T | 1.534 | 1.00 |
| 250_L | 272_F | 1.527 | 1.00 |
| 178_F | 182_C | 1.519 | 1.00 |
| 144_M | 158_S | 1.505 | 1.00 |
| 38_S | 41_K | 1.499 | 1.00 |
| 280_P | 283_D | 1.497 | 1.00 |
| 192_L | 196_A | 1.477 | 1.00 |
| 21_L | 24_L | 1.469 | 1.00 |
| 118_K | 122_S | 1.463 | 1.00 |
| 122_S | 126_L | 1.442 | 1.00 |
| 121_Q | 125_G | 1.436 | 1.00 |
| 83_Y | 132_S | 1.425 | 1.00 |
| 106_A | 110_P | 1.413 | 1.00 |
| 184_F | 245_T | 1.390 | 0.99 |
| 199_I | 230_L | 1.389 | 0.99 |
| 244_W | 283_D | 1.379 | 0.99 |
| 229_T | 232_M | 1.363 | 0.99 |
| 10_F | 60_P | 1.361 | 0.99 |
| 197_K | 201_T | 1.349 | 0.99 |
| 14_A | 55_T | 1.346 | 0.99 |
| 54_E | 92_P | 1.342 | 0.99 |
| 20_F | 288_G | 1.337 | 0.99 |
| 249_V | 253_W | 1.334 | 0.99 |
| 96_M | 191_M | 1.334 | 0.99 |
| 146_H | 158_S | 1.331 | 0.99 |
| 104_S | 108_T | 1.320 | 0.99 |
| 191_M | 237_A | 1.312 | 0.99 |
| 264_L | 268_V | 1.308 | 0.99 |
| 18_A | 52_L | 1.307 | 0.99 |
| 177_F | 256_F | 1.298 | 0.99 |
| 245_T | 249_V | 1.290 | 0.99 |
| 74_G | 77_L | 1.278 | 0.99 |
| 241_T | 245_T | 1.275 | 0.99 |
| 94_F | 97_V | 1.269 | 0.99 |
| 247_Y | 276_A | 1.262 | 0.99 |
| 184_F | 241_T | 1.256 | 0.99 |
| 50_A | 128_W | 1.248 | 0.99 |
| 90_Y | 183_L | 1.248 | 0.99 |
| 51_N | 283_D | 1.247 | 0.99 |
| 45_K | 49_L | 1.238 | 0.98 |
| 20_F | 287_Y | 1.238 | 0.98 |
| 93_A | 97_V | 1.236 | 0.98 |
| 93_A | 240_F | 1.232 | 0.98 |
| 235_A | 286_I | 1.221 | 0.98 |
| 126_L | 130_L | 1.219 | 0.98 |
| 268_V | 272_F | 1.211 | 0.98 |
| 10_F | 59_M | 1.204 | 0.98 |
| 104_S | 193_I | 1.187 | 0.98 |
| 199_I | 203_T | 1.178 | 0.98 |
| 61_L | 77_L | 1.178 | 0.98 |
| 191_M | 195_N | 1.172 | 0.98 |
| 109_R | 112_A | 1.164 | 0.97 |
| 15_T | 18_A | 1.164 | 0.97 |
| 53_L | 88_S | 1.161 | 0.97 |
| 46_H | 124_V | 1.153 | 0.97 |
| 7_F | 63_G | 1.149 | 0.97 |
| 13_S | 281_C | 1.136 | 0.97 |
| 12_L | 15_T | 1.134 | 0.97 |
| 10_F | 63_G | 1.132 | 0.97 |
| 102_D | 228_K | 1.120 | 0.96 |
| 63_G | 67_I | 1.118 | 0.96 |
| 191_M | 194_C | 1.117 | 0.96 |
| 234_V | 238_T | 1.107 | 0.96 |
| 64_M | 72_Y | 1.098 | 0.96 |
| 16_F | 20_F | 1.095 | 0.96 |
| 99_I | 233_T | 1.094 | 0.96 |
| 136_G | 139_L | 1.083 | 0.96 |
| 122_S | 125_G | 1.080 | 0.95 |
| 91_A | 95_M | 1.079 | 0.95 |
| 251_G | 255_W | 1.073 | 0.95 |
| 78_C | 160_C | 1.073 | 0.95 |
| 74_G | 79_K | 1.072 | 0.95 |
| 288_G | 291_S | 1.070 | 0.95 |
| 7_F | 67_I | 1.070 | 0.95 |
| 74_G | 78_C | 1.069 | 0.95 |
| 47_L | 51_N | 1.068 | 0.95 |
| 43_L | 99_I | 1.061 | 0.95 |
| 93_A | 248_Y | 1.060 | 0.95 |
| 94_F | 130_L | 1.060 | 0.95 |
| 91_A | 128_W | 1.059 | 0.95 |
| 283_D | 291_S | 1.059 | 0.95 |
| 21_L | 49_L | 1.054 | 0.95 |
| 73_A | 77_L | 1.052 | 0.95 |
| 202_L | 229_T | 1.048 | 0.94 |
| 280_P | 284_P | 1.034 | 0.94 |
| 239_S | 282_F | 1.025 | 0.93 |
| 12_L | 285_L | 1.024 | 0.93 |
| 26_K | 33_K | 1.024 | 0.93 |
| 14_A | 52_L | 1.023 | 0.93 |
| 86_L | 248_Y | 1.021 | 0.93 |
| 21_L | 48_T | 1.004 | 0.93 |
| 39_R | 43_L | 1.001 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4iarA | 1 | 0.8938 | 100 | 0.162 | Contact Map |
| 4bvnA | 1 | 0.9178 | 100 | 0.194 | Contact Map |
| 4pxzA | 1 | 0.9315 | 100 | 0.194 | Contact Map |
| 2rh1A | 1 | 0.9418 | 100 | 0.195 | Contact Map |
| 3uonA | 1 | 0.9384 | 100 | 0.197 | Contact Map |
| 3oduA | 2 | 0.9418 | 100 | 0.205 | Contact Map |
| 3pblA | 2 | 0.911 | 100 | 0.207 | Contact Map |
| 3vw7A | 1 | 0.9452 | 100 | 0.21 | Contact Map |
| 4dklA | 1 | 0.9521 | 100 | 0.211 | Contact Map |
| 3rzeA | 1 | 0.9144 | 100 | 0.213 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)