| gpcr_1e-40 |
| ID: | 1501558935 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 292 (281) | ||
| Sequences: | 10377 (5839.5) | ||
| Seq/Len: | 36.929 | ||
| Nf(neff/√len): | 348.4 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 36.929).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 13_S | 55_T | 5.115 | 1.00 |
| 193_I | 197_K | 3.385 | 1.00 |
| 76_L | 80_V | 3.269 | 1.00 |
| 46_H | 95_M | 3.047 | 1.00 |
| 104_S | 109_R | 2.882 | 1.00 |
| 75_E | 79_K | 2.858 | 1.00 |
| 46_H | 128_W | 2.772 | 1.00 |
| 98_V | 127_A | 2.748 | 1.00 |
| 71_W | 74_G | 2.472 | 1.00 |
| 196_A | 200_F | 2.267 | 1.00 |
| 18_A | 22_L | 2.258 | 1.00 |
| 98_V | 123_M | 2.164 | 1.00 |
| 87_F | 132_S | 2.164 | 1.00 |
| 197_K | 200_F | 2.112 | 1.00 |
| 244_W | 276_A | 2.108 | 1.00 |
| 104_S | 197_K | 2.069 | 1.00 |
| 83_Y | 87_F | 2.066 | 1.00 |
| 190_I | 194_C | 2.062 | 1.00 |
| 239_S | 286_I | 2.059 | 1.00 |
| 24_L | 45_K | 2.031 | 1.00 |
| 21_L | 45_K | 2.029 | 1.00 |
| 42_L | 124_V | 1.885 | 1.00 |
| 192_L | 196_A | 1.837 | 1.00 |
| 118_K | 122_S | 1.833 | 1.00 |
| 64_M | 73_A | 1.816 | 1.00 |
| 178_F | 182_C | 1.777 | 1.00 |
| 232_M | 290_F | 1.772 | 1.00 |
| 21_L | 52_L | 1.714 | 1.00 |
| 267_P | 271_F | 1.705 | 1.00 |
| 95_M | 124_V | 1.705 | 1.00 |
| 43_L | 120_G | 1.704 | 1.00 |
| 250_L | 272_F | 1.702 | 1.00 |
| 249_V | 253_W | 1.679 | 1.00 |
| 200_F | 204_R | 1.652 | 1.00 |
| 10_F | 63_G | 1.634 | 1.00 |
| 122_S | 126_L | 1.609 | 1.00 |
| 75_E | 143_R | 1.588 | 1.00 |
| 83_Y | 139_L | 1.587 | 1.00 |
| 18_A | 52_L | 1.578 | 1.00 |
| 180_F | 185_I | 1.575 | 1.00 |
| 38_S | 41_K | 1.563 | 1.00 |
| 136_G | 140_Y | 1.561 | 1.00 |
| 101_L | 194_C | 1.558 | 1.00 |
| 48_T | 51_N | 1.557 | 1.00 |
| 91_A | 131_S | 1.518 | 1.00 |
| 36_K | 41_K | 1.513 | 1.00 |
| 121_Q | 125_G | 1.508 | 1.00 |
| 184_F | 245_T | 1.508 | 1.00 |
| 278_L | 282_F | 1.498 | 1.00 |
| 45_K | 49_L | 1.496 | 1.00 |
| 46_H | 124_V | 1.495 | 1.00 |
| 96_M | 191_M | 1.484 | 1.00 |
| 180_F | 252_I | 1.479 | 1.00 |
| 74_G | 77_L | 1.464 | 1.00 |
| 78_C | 160_C | 1.456 | 1.00 |
| 188_L | 241_T | 1.451 | 1.00 |
| 177_F | 256_F | 1.441 | 1.00 |
| 247_Y | 251_G | 1.429 | 1.00 |
| 53_L | 88_S | 1.426 | 1.00 |
| 14_A | 55_T | 1.408 | 1.00 |
| 123_M | 127_A | 1.404 | 0.99 |
| 250_L | 268_V | 1.403 | 0.99 |
| 245_T | 249_V | 1.399 | 0.99 |
| 268_V | 272_F | 1.398 | 0.99 |
| 104_S | 108_T | 1.397 | 0.99 |
| 50_A | 91_A | 1.394 | 0.99 |
| 104_S | 193_I | 1.385 | 0.99 |
| 241_T | 245_T | 1.385 | 0.99 |
| 109_R | 112_A | 1.368 | 0.99 |
| 239_S | 282_F | 1.361 | 0.99 |
| 21_L | 48_T | 1.357 | 0.99 |
| 94_F | 130_L | 1.347 | 0.99 |
| 102_D | 106_A | 1.340 | 0.99 |
| 197_K | 201_T | 1.335 | 0.99 |
| 144_M | 158_S | 1.317 | 0.99 |
| 21_L | 24_L | 1.314 | 0.99 |
| 10_F | 60_P | 1.307 | 0.99 |
| 7_F | 63_G | 1.306 | 0.99 |
| 51_N | 283_D | 1.303 | 0.99 |
| 61_L | 77_L | 1.285 | 0.99 |
| 283_D | 287_Y | 1.282 | 0.99 |
| 94_F | 97_V | 1.277 | 0.99 |
| 199_I | 230_L | 1.268 | 0.99 |
| 105_L | 109_R | 1.260 | 0.99 |
| 199_I | 203_T | 1.250 | 0.99 |
| 184_F | 240_F | 1.250 | 0.99 |
| 91_A | 95_M | 1.230 | 0.98 |
| 14_A | 52_L | 1.230 | 0.98 |
| 235_A | 286_I | 1.228 | 0.98 |
| 106_A | 110_P | 1.226 | 0.98 |
| 64_M | 72_Y | 1.216 | 0.98 |
| 12_L | 15_T | 1.213 | 0.98 |
| 91_A | 128_W | 1.213 | 0.98 |
| 264_L | 268_V | 1.210 | 0.98 |
| 247_Y | 276_A | 1.209 | 0.98 |
| 39_R | 120_G | 1.203 | 0.98 |
| 16_F | 281_C | 1.179 | 0.98 |
| 90_Y | 183_L | 1.164 | 0.97 |
| 106_A | 111_L | 1.153 | 0.97 |
| 108_T | 201_T | 1.142 | 0.97 |
| 13_S | 281_C | 1.139 | 0.97 |
| 202_L | 229_T | 1.135 | 0.97 |
| 10_F | 59_M | 1.134 | 0.97 |
| 195_N | 198_I | 1.128 | 0.97 |
| 279_N | 283_D | 1.121 | 0.97 |
| 146_H | 158_S | 1.113 | 0.96 |
| 9_L | 278_L | 1.110 | 0.96 |
| 234_V | 238_T | 1.110 | 0.96 |
| 50_A | 128_W | 1.106 | 0.96 |
| 126_L | 130_L | 1.102 | 0.96 |
| 16_F | 20_F | 1.102 | 0.96 |
| 73_A | 77_L | 1.096 | 0.96 |
| 99_I | 233_T | 1.095 | 0.96 |
| 229_T | 232_M | 1.092 | 0.96 |
| 54_E | 280_P | 1.089 | 0.96 |
| 26_K | 33_K | 1.087 | 0.96 |
| 288_G | 291_S | 1.085 | 0.96 |
| 21_L | 49_L | 1.083 | 0.96 |
| 83_Y | 132_S | 1.073 | 0.95 |
| 7_F | 67_I | 1.072 | 0.95 |
| 19_S | 23_K | 1.070 | 0.95 |
| 119_V | 122_S | 1.066 | 0.95 |
| 97_V | 190_I | 1.064 | 0.95 |
| 15_T | 18_A | 1.062 | 0.95 |
| 39_R | 43_L | 1.061 | 0.95 |
| 41_K | 44_L | 1.053 | 0.95 |
| 46_H | 49_L | 1.053 | 0.95 |
| 122_S | 125_G | 1.042 | 0.94 |
| 191_M | 237_A | 1.039 | 0.94 |
| 188_L | 245_T | 1.028 | 0.94 |
| 33_K | 36_K | 1.028 | 0.94 |
| 201_T | 204_R | 1.028 | 0.94 |
| 3_V | 7_F | 1.018 | 0.93 |
| 82_S | 142_F | 1.008 | 0.93 |
| 50_A | 92_P | 1.003 | 0.93 |
| 47_L | 51_N | 1.001 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4iarA | 1 | 0.8938 | 100 | 0.142 | Contact Map |
| 4pxzA | 1 | 0.9315 | 100 | 0.17 | Contact Map |
| 4bvnA | 1 | 0.9178 | 100 | 0.17 | Contact Map |
| 2rh1A | 1 | 0.9418 | 100 | 0.172 | Contact Map |
| 3uonA | 1 | 0.9384 | 100 | 0.179 | Contact Map |
| 3oduA | 2 | 0.9418 | 100 | 0.185 | Contact Map |
| 3vw7A | 1 | 0.9452 | 100 | 0.186 | Contact Map |
| 3pblA | 2 | 0.911 | 100 | 0.188 | Contact Map |
| 4dklA | 1 | 0.9521 | 100 | 0.189 | Contact Map |
| 3emlA | 1 | 0.9384 | 100 | 0.194 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)