| enniatin |
| ID: | 1498568949 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 679 (639) | ||
| Sequences: | 13625 (11563.3) | ||
| Seq/Len: | 21.322 | ||
| Nf(neff/√len): | 457.4 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 21.322).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 629_L | 646_M | 4.980 | 1.00 |
| 537_I | 563_T | 3.565 | 1.00 |
| 294_E | 324_R | 3.468 | 1.00 |
| 535_H | 628_D | 3.350 | 1.00 |
| 93_C | 107_L | 3.256 | 1.00 |
| 331_Q | 339_T | 3.077 | 1.00 |
| 580_Q | 634_F | 3.007 | 1.00 |
| 516_D | 557_K | 2.969 | 1.00 |
| 565_I | 574_T | 2.903 | 1.00 |
| 66_E | 154_E | 2.772 | 1.00 |
| 520_S | 557_K | 2.762 | 1.00 |
| 525_H | 529_I | 2.739 | 1.00 |
| 648_E | 651_S | 2.659 | 1.00 |
| 233_H | 343_Y | 2.555 | 1.00 |
| 536_A | 584_T | 2.548 | 1.00 |
| 539_P | 583_A | 2.510 | 1.00 |
| 493_W | 497_S | 2.405 | 1.00 |
| 491_L | 516_D | 2.399 | 1.00 |
| 531_L | 560_H | 2.371 | 1.00 |
| 333_V | 449_L | 2.364 | 1.00 |
| 513_A | 517_N | 2.362 | 1.00 |
| 576_Q | 641_I | 2.346 | 1.00 |
| 289_Y | 441_Q | 2.342 | 1.00 |
| 484_T | 492_K | 2.320 | 1.00 |
| 522_L | 550_A | 2.320 | 1.00 |
| 285_L | 445_L | 2.298 | 1.00 |
| 283_A | 325_V | 2.293 | 1.00 |
| 493_W | 496_Q | 2.266 | 1.00 |
| 533_K | 628_D | 2.259 | 1.00 |
| 516_D | 619_E | 2.234 | 1.00 |
| 548_V | 677_H | 2.209 | 1.00 |
| 77_A | 81_K | 2.206 | 1.00 |
| 537_I | 632_V | 2.204 | 1.00 |
| 270_Q | 334_S | 2.201 | 1.00 |
| 81_K | 85_L | 2.191 | 1.00 |
| 59_A | 62_H | 2.191 | 1.00 |
| 520_S | 524_Q | 2.163 | 1.00 |
| 226_A | 350_F | 2.157 | 1.00 |
| 501_A | 509_E | 2.133 | 1.00 |
| 655_C | 674_F | 2.114 | 1.00 |
| 432_A | 436_A | 2.097 | 1.00 |
| 86_H | 177_R | 2.088 | 1.00 |
| 537_I | 561_A | 2.088 | 1.00 |
| 423_A | 439_L | 2.076 | 1.00 |
| 655_C | 659_F | 2.073 | 1.00 |
| 497_S | 500_K | 2.042 | 1.00 |
| 531_L | 536_A | 2.023 | 1.00 |
| 485_L | 488_S | 2.012 | 1.00 |
| 68_P | 154_E | 2.004 | 1.00 |
| 161_F | 166_V | 2.002 | 1.00 |
| 519_S | 557_K | 1.935 | 1.00 |
| 83_T | 181_A | 1.924 | 1.00 |
| 627_H | 648_E | 1.918 | 1.00 |
| 625_K | 628_D | 1.917 | 1.00 |
| 578_V | 583_A | 1.904 | 1.00 |
| 665_I | 669_T | 1.895 | 1.00 |
| 294_E | 326_S | 1.885 | 1.00 |
| 296_L | 322_P | 1.852 | 1.00 |
| 505_A | 546_K | 1.848 | 1.00 |
| 298_G | 371_F | 1.826 | 1.00 |
| 83_T | 178_V | 1.824 | 1.00 |
| 666_T | 669_T | 1.822 | 1.00 |
| 518_V | 547_W | 1.811 | 1.00 |
| 423_A | 435_M | 1.806 | 1.00 |
| 532_G | 536_A | 1.803 | 1.00 |
| 332_S | 335_D | 1.796 | 1.00 |
| 522_L | 554_A | 1.777 | 1.00 |
| 499_N | 513_A | 1.773 | 1.00 |
| 282_L | 373_T | 1.771 | 1.00 |
| 536_A | 560_H | 1.749 | 1.00 |
| 448_N | 461_D | 1.749 | 1.00 |
| 280_T | 336_I | 1.737 | 1.00 |
| 516_D | 520_S | 1.734 | 1.00 |
| 334_S | 455_L | 1.726 | 1.00 |
| 627_H | 650_R | 1.721 | 1.00 |
| 510_W | 550_A | 1.698 | 1.00 |
| 551_S | 587_L | 1.697 | 1.00 |
| 659_F | 675_G | 1.693 | 1.00 |
| 76_L | 178_V | 1.689 | 1.00 |
| 82_E | 85_L | 1.689 | 1.00 |
| 176_R | 312_M | 1.687 | 1.00 |
| 540_I | 551_S | 1.687 | 1.00 |
| 571_P | 575_A | 1.681 | 1.00 |
| 487_H | 649_H | 1.667 | 1.00 |
| 491_L | 557_K | 1.667 | 1.00 |
| 576_Q | 634_F | 1.662 | 1.00 |
| 510_W | 546_K | 1.660 | 1.00 |
| 227_V | 353_A | 1.650 | 1.00 |
| 80_W | 144_F | 1.646 | 1.00 |
| 174_I | 314_D | 1.645 | 1.00 |
| 441_Q | 467_D | 1.644 | 1.00 |
| 575_A | 579_Q | 1.642 | 1.00 |
| 281_A | 446_I | 1.641 | 1.00 |
| 554_A | 558_A | 1.640 | 1.00 |
| 492_K | 496_Q | 1.637 | 1.00 |
| 658_K | 674_F | 1.633 | 1.00 |
| 150_P | 153_K | 1.628 | 1.00 |
| 412_C | 421_L | 1.625 | 1.00 |
| 501_A | 504_A | 1.625 | 1.00 |
| 87_T | 177_R | 1.622 | 1.00 |
| 93_C | 139_P | 1.621 | 1.00 |
| 41_G | 102_V | 1.606 | 1.00 |
| 486_I | 652_F | 1.598 | 1.00 |
| 276_T | 341_D | 1.587 | 1.00 |
| 82_E | 185_A | 1.584 | 1.00 |
| 505_A | 508_G | 1.581 | 1.00 |
| 580_Q | 641_I | 1.576 | 1.00 |
| 229_F | 347_M | 1.565 | 1.00 |
| 655_C | 658_K | 1.553 | 1.00 |
| 490_M | 553_L | 1.552 | 1.00 |
| 569_D | 577_V | 1.552 | 1.00 |
| 228_E | 232_D | 1.551 | 1.00 |
| 655_C | 673_Q | 1.548 | 1.00 |
| 659_F | 674_F | 1.539 | 1.00 |
| 237_L | 296_L | 1.534 | 1.00 |
| 655_C | 675_G | 1.533 | 1.00 |
| 443_G | 447_Q | 1.531 | 1.00 |
| 547_W | 589_S | 1.527 | 1.00 |
| 266_S | 277_I | 1.522 | 1.00 |
| 569_D | 678_A | 1.519 | 1.00 |
| 325_V | 340_I | 1.518 | 1.00 |
| 484_T | 488_S | 1.515 | 1.00 |
| 72_D | 75_R | 1.504 | 1.00 |
| 410_L | 423_A | 1.501 | 1.00 |
| 287_S | 327_C | 1.500 | 1.00 |
| 624_V | 628_D | 1.497 | 1.00 |
| 589_S | 592_H | 1.497 | 1.00 |
| 245_F | 248_S | 1.490 | 1.00 |
| 82_E | 181_A | 1.488 | 1.00 |
| 563_T | 581_T | 1.484 | 1.00 |
| 227_V | 231_K | 1.476 | 1.00 |
| 489_E | 653_S | 1.473 | 1.00 |
| 357_N | 360_S | 1.468 | 1.00 |
| 461_D | 468_W | 1.451 | 1.00 |
| 141_C | 165_L | 1.450 | 1.00 |
| 671_A | 674_F | 1.445 | 1.00 |
| 490_M | 494_T | 1.445 | 1.00 |
| 282_L | 410_L | 1.436 | 1.00 |
| 672_L | 675_G | 1.432 | 1.00 |
| 280_T | 340_I | 1.428 | 1.00 |
| 211_P | 214_M | 1.419 | 1.00 |
| 571_P | 595_T | 1.415 | 1.00 |
| 531_L | 538_V | 1.410 | 1.00 |
| 579_Q | 641_I | 1.401 | 0.99 |
| 342_S | 346_T | 1.400 | 0.99 |
| 425_Y | 435_M | 1.398 | 0.99 |
| 328_A | 331_Q | 1.396 | 0.99 |
| 540_I | 552_M | 1.389 | 0.99 |
| 283_A | 297_F | 1.387 | 0.99 |
| 289_Y | 438_L | 1.381 | 0.99 |
| 75_R | 185_A | 1.380 | 0.99 |
| 263_S | 266_S | 1.377 | 0.99 |
| 511_T | 514_E | 1.375 | 0.99 |
| 469_L | 473_R | 1.375 | 0.99 |
| 658_K | 671_A | 1.373 | 0.99 |
| 436_A | 440_R | 1.373 | 0.99 |
| 431_D | 434_Q | 1.371 | 0.99 |
| 503_V | 546_K | 1.370 | 0.99 |
| 437_R | 441_Q | 1.368 | 0.99 |
| 503_V | 549_V | 1.365 | 0.99 |
| 232_D | 235_S | 1.361 | 0.99 |
| 588_T | 592_H | 1.354 | 0.99 |
| 274_S | 419_A | 1.352 | 0.99 |
| 490_M | 516_D | 1.351 | 0.99 |
| 654_S | 659_F | 1.349 | 0.99 |
| 224_D | 228_E | 1.344 | 0.99 |
| 489_E | 657_I | 1.343 | 0.99 |
| 66_E | 156_Q | 1.342 | 0.99 |
| 535_H | 629_L | 1.342 | 0.99 |
| 543_E | 589_S | 1.341 | 0.99 |
| 671_A | 675_G | 1.339 | 0.99 |
| 456_P | 459_K | 1.336 | 0.99 |
| 130_D | 133_A | 1.333 | 0.99 |
| 92_T | 106_V | 1.332 | 0.99 |
| 576_Q | 580_Q | 1.331 | 0.99 |
| 516_D | 519_S | 1.329 | 0.99 |
| 94_T | 165_L | 1.329 | 0.99 |
| 226_A | 352_H | 1.328 | 0.99 |
| 430_I | 435_M | 1.326 | 0.99 |
| 561_A | 629_L | 1.314 | 0.99 |
| 504_A | 509_E | 1.310 | 0.99 |
| 658_K | 663_L | 1.303 | 0.99 |
| 581_T | 634_F | 1.295 | 0.99 |
| 542_F | 551_S | 1.293 | 0.99 |
| 53_A | 96_T | 1.290 | 0.99 |
| 655_C | 663_L | 1.287 | 0.99 |
| 524_Q | 620_L | 1.282 | 0.99 |
| 656_A | 675_G | 1.277 | 0.99 |
| 487_H | 624_V | 1.270 | 0.99 |
| 70_D | 153_K | 1.269 | 0.99 |
| 284_I | 336_I | 1.267 | 0.99 |
| 491_L | 519_S | 1.265 | 0.99 |
| 281_A | 449_L | 1.264 | 0.99 |
| 525_H | 528_S | 1.260 | 0.99 |
| 270_Q | 338_S | 1.258 | 0.99 |
| 285_L | 442_L | 1.254 | 0.99 |
| 272_M | 337_M | 1.254 | 0.99 |
| 494_T | 516_D | 1.246 | 0.98 |
| 73_A | 146_L | 1.242 | 0.98 |
| 364_D | 367_A | 1.241 | 0.98 |
| 218_P | 221_A | 1.240 | 0.98 |
| 122_D | 125_E | 1.235 | 0.98 |
| 144_F | 157_L | 1.232 | 0.98 |
| 233_H | 346_T | 1.232 | 0.98 |
| 38_I | 103_L | 1.231 | 0.98 |
| 663_L | 674_F | 1.229 | 0.98 |
| 284_I | 445_L | 1.228 | 0.98 |
| 572_A | 576_Q | 1.223 | 0.98 |
| 508_G | 546_K | 1.221 | 0.98 |
| 658_K | 662_A | 1.220 | 0.98 |
| 631_Y | 647_I | 1.219 | 0.98 |
| 556_L | 630_A | 1.218 | 0.98 |
| 659_F | 662_A | 1.214 | 0.98 |
| 358_I | 361_A | 1.212 | 0.98 |
| 518_V | 550_A | 1.209 | 0.98 |
| 176_R | 180_K | 1.204 | 0.98 |
| 214_M | 217_I | 1.204 | 0.98 |
| 651_S | 654_S | 1.204 | 0.98 |
| 359_A | 362_G | 1.202 | 0.98 |
| 46_R | 101_D | 1.201 | 0.98 |
| 95_F | 105_V | 1.201 | 0.98 |
| 88_P | 210_N | 1.197 | 0.98 |
| 94_T | 164_A | 1.193 | 0.98 |
| 326_S | 331_Q | 1.192 | 0.98 |
| 242_L | 245_F | 1.177 | 0.98 |
| 71_I | 155_R | 1.176 | 0.98 |
| 654_S | 675_G | 1.175 | 0.98 |
| 76_L | 157_L | 1.172 | 0.98 |
| 659_F | 663_L | 1.170 | 0.97 |
| 652_F | 655_C | 1.170 | 0.97 |
| 327_C | 331_Q | 1.165 | 0.97 |
| 71_I | 76_L | 1.161 | 0.97 |
| 348_R | 351_A | 1.160 | 0.97 |
| 229_F | 233_H | 1.154 | 0.97 |
| 279_R | 297_F | 1.154 | 0.97 |
| 85_L | 109_D | 1.154 | 0.97 |
| 501_A | 511_T | 1.151 | 0.97 |
| 48_V | 316_P | 1.145 | 0.97 |
| 508_G | 518_V | 1.142 | 0.97 |
| 125_E | 129_Q | 1.138 | 0.97 |
| 656_A | 660_G | 1.136 | 0.97 |
| 322_P | 358_I | 1.132 | 0.97 |
| 647_I | 651_S | 1.131 | 0.97 |
| 335_D | 338_S | 1.129 | 0.97 |
| 487_H | 556_L | 1.128 | 0.97 |
| 181_A | 185_A | 1.126 | 0.97 |
| 278_C | 419_A | 1.124 | 0.97 |
| 658_K | 675_G | 1.122 | 0.97 |
| 72_D | 182_Y | 1.119 | 0.96 |
| 486_I | 556_L | 1.118 | 0.96 |
| 656_A | 671_A | 1.116 | 0.96 |
| 272_M | 276_T | 1.115 | 0.96 |
| 78_A | 185_A | 1.114 | 0.96 |
| 651_S | 655_C | 1.113 | 0.96 |
| 170_F | 314_D | 1.112 | 0.96 |
| 572_A | 641_I | 1.107 | 0.96 |
| 551_S | 555_V | 1.107 | 0.96 |
| 518_V | 521_R | 1.105 | 0.96 |
| 126_A | 147_L | 1.102 | 0.96 |
| 92_T | 104_Q | 1.101 | 0.96 |
| 539_P | 565_I | 1.100 | 0.96 |
| 655_C | 672_L | 1.099 | 0.96 |
| 248_S | 251_Y | 1.099 | 0.96 |
| 244_A | 247_L | 1.097 | 0.96 |
| 655_C | 671_A | 1.096 | 0.96 |
| 224_D | 227_V | 1.091 | 0.96 |
| 148_E | 155_R | 1.091 | 0.96 |
| 508_G | 547_W | 1.090 | 0.96 |
| 658_K | 676_S | 1.089 | 0.96 |
| 444_H | 448_N | 1.088 | 0.96 |
| 491_L | 520_S | 1.085 | 0.96 |
| 355_L | 359_A | 1.081 | 0.95 |
| 89_A | 92_T | 1.077 | 0.95 |
| 541_Y | 574_T | 1.076 | 0.95 |
| 330_E | 458_E | 1.075 | 0.95 |
| 440_R | 464_T | 1.074 | 0.95 |
| 282_L | 375_L | 1.071 | 0.95 |
| 629_L | 648_E | 1.071 | 0.95 |
| 250_V | 253_H | 1.069 | 0.95 |
| 569_D | 574_T | 1.066 | 0.95 |
| 276_T | 337_M | 1.062 | 0.95 |
| 519_S | 554_A | 1.061 | 0.95 |
| 241_C | 244_A | 1.061 | 0.95 |
| 64_V | 156_Q | 1.061 | 0.95 |
| 564_L | 633_I | 1.061 | 0.95 |
| 146_L | 157_L | 1.060 | 0.95 |
| 654_S | 658_K | 1.059 | 0.95 |
| 250_V | 254_P | 1.059 | 0.95 |
| 634_F | 644_G | 1.056 | 0.95 |
| 287_S | 290_T | 1.054 | 0.95 |
| 126_A | 130_D | 1.052 | 0.95 |
| 673_Q | 676_S | 1.051 | 0.94 |
| 279_R | 283_A | 1.049 | 0.94 |
| 539_P | 581_T | 1.048 | 0.94 |
| 350_F | 354_G | 1.047 | 0.94 |
| 659_F | 673_Q | 1.044 | 0.94 |
| 246_V | 249_S | 1.041 | 0.94 |
| 488_S | 491_L | 1.041 | 0.94 |
| 210_N | 213_K | 1.040 | 0.94 |
| 538_V | 560_H | 1.039 | 0.94 |
| 339_T | 343_Y | 1.034 | 0.94 |
| 264_Y | 267_S | 1.030 | 0.94 |
| 665_I | 671_A | 1.030 | 0.94 |
| 344_D | 348_R | 1.022 | 0.93 |
| 492_K | 495_S | 1.022 | 0.93 |
| 209_P | 213_K | 1.021 | 0.93 |
| 278_C | 442_L | 1.019 | 0.93 |
| 515_L | 550_A | 1.018 | 0.93 |
| 132_A | 135_A | 1.015 | 0.93 |
| 228_E | 231_K | 1.013 | 0.93 |
| 278_C | 375_L | 1.011 | 0.93 |
| 229_F | 346_T | 1.005 | 0.93 |
| 124_K | 128_V | 1.002 | 0.92 |
| 350_F | 355_L | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2vsqA | 1 | 0.9234 | 100 | 0.248 | Contact Map |
| 4tx3B | 1 | 0.6186 | 100 | 0.576 | Contact Map |
| 4hvmA | 4 | 0.5493 | 100 | 0.579 | Contact Map |
| 2jgpA | 1 | 0.6392 | 100 | 0.579 | Contact Map |
| 1l5aA | 3 | 0.592 | 100 | 0.582 | Contact Map |
| 4jn3A | 2 | 0.6038 | 100 | 0.588 | Contact Map |
| 4znmA | 2 | 0 | 100 | 0.598 | Contact Map |
| 2xhgA | 1 | 0.6097 | 100 | 0.624 | Contact Map |
| 1q9jA | 2 | 0.5317 | 100 | 0.669 | Contact Map |
| 4dg8A | 1 | 0.2931 | 100 | 0.689 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)