| ASNB_ECOLI Asparagine synthetase B |
| ID: | 1498046644 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 554 (526) | ||
| Sequences: | 2279 (1227.5) | ||
| Seq/Len: | 4.333 | ||
| Nf(neff/√len): | 53.5 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 4.333).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 35_W | 60_A | 4.121 | 1.00 |
| 285_Q | 297_E | 4.119 | 1.00 |
| 25_S | 47_A | 3.886 | 1.00 |
| 269_H | 294_V | 3.470 | 1.00 |
| 215_A | 412_V | 3.431 | 1.00 |
| 106_L | 110_K | 3.418 | 1.00 |
| 143_I | 402_V | 3.409 | 1.00 |
| 21_A | 45_I | 3.374 | 1.00 |
| 141_L | 410_L | 3.331 | 1.00 |
| 87_E | 117_D | 3.316 | 1.00 |
| 92_Y | 109_E | 3.122 | 1.00 |
| 59_G | 98_S | 3.106 | 1.00 |
| 242_I | 437_F | 3.067 | 1.00 |
| 92_Y | 105_A | 2.854 | 1.00 |
| 65_N | 69_T | 2.812 | 1.00 |
| 136_I | 146_L | 2.801 | 1.00 |
| 110_K | 117_D | 2.786 | 1.00 |
| 65_N | 108_Q | 2.769 | 1.00 |
| 286_E | 444_S | 2.701 | 1.00 |
| 217_E | 249_K | 2.686 | 1.00 |
| 218_D | 408_K | 2.669 | 1.00 |
| 216_L | 246_I | 2.652 | 1.00 |
| 393_M | 400_A | 2.637 | 1.00 |
| 232_V | 244_S | 2.611 | 1.00 |
| 143_I | 223_H | 2.579 | 1.00 |
| 64_Y | 71_V | 2.467 | 1.00 |
| 119_Q | 222_S | 2.429 | 1.00 |
| 125_A | 136_I | 2.362 | 1.00 |
| 269_H | 296_H | 2.315 | 1.00 |
| 213_R | 436_C | 2.303 | 1.00 |
| 106_L | 114_F | 2.302 | 1.00 |
| 140_H | 407_K | 2.300 | 1.00 |
| 407_K | 411_D | 2.294 | 1.00 |
| 72_L | 100_C | 2.289 | 1.00 |
| 338_K | 343_K | 2.240 | 1.00 |
| 435_E | 438_E | 2.234 | 1.00 |
| 21_A | 47_A | 2.207 | 1.00 |
| 70_H | 104_L | 2.173 | 1.00 |
| 121_M | 143_I | 2.173 | 1.00 |
| 355_F | 413_A | 2.160 | 1.00 |
| 270_S | 293_T | 2.127 | 1.00 |
| 114_F | 118_L | 2.089 | 1.00 |
| 36_S | 49_E | 2.063 | 1.00 |
| 212_L | 412_V | 2.059 | 1.00 |
| 213_R | 439_A | 1.988 | 1.00 |
| 231_G | 342_I | 1.959 | 1.00 |
| 106_L | 118_L | 1.934 | 1.00 |
| 163_K | 317_E | 1.915 | 1.00 |
| 119_Q | 223_H | 1.910 | 1.00 |
| 351_S | 404_F | 1.904 | 1.00 |
| 438_E | 447_W | 1.894 | 1.00 |
| 121_M | 223_H | 1.892 | 1.00 |
| 213_R | 440_Y | 1.878 | 1.00 |
| 115_L | 180_Y | 1.877 | 1.00 |
| 222_S | 408_K | 1.863 | 1.00 |
| 159_A | 164_A | 1.841 | 1.00 |
| 119_Q | 407_K | 1.836 | 1.00 |
| 5_F | 24_L | 1.823 | 1.00 |
| 74_V | 78_I | 1.809 | 1.00 |
| 244_S | 293_T | 1.807 | 1.00 |
| 270_S | 295_H | 1.806 | 1.00 |
| 211_E | 215_A | 1.796 | 1.00 |
| 180_Y | 189_R | 1.795 | 1.00 |
| 303_Q | 307_D | 1.791 | 1.00 |
| 423_C | 431_H | 1.767 | 1.00 |
| 212_L | 355_F | 1.749 | 0.99 |
| 241_S | 287_V | 1.744 | 0.99 |
| 122_F | 137_G | 1.731 | 0.99 |
| 54_V | 81_H | 1.730 | 0.99 |
| 107_Y | 126_L | 1.729 | 0.99 |
| 311_D | 335_R | 1.722 | 0.99 |
| 82_Q | 225_M | 1.717 | 0.99 |
| 148_M | 173_K | 1.690 | 0.99 |
| 115_L | 124_F | 1.673 | 0.99 |
| 285_Q | 289_N | 1.670 | 0.99 |
| 431_H | 435_E | 1.659 | 0.99 |
| 65_N | 105_A | 1.655 | 0.99 |
| 119_Q | 218_D | 1.654 | 0.99 |
| 115_L | 137_G | 1.649 | 0.99 |
| 324_I | 388_R | 1.645 | 0.99 |
| 35_W | 52_S | 1.643 | 0.99 |
| 107_Y | 135_L | 1.625 | 0.99 |
| 349_E | 388_R | 1.623 | 0.99 |
| 439_A | 443_A | 1.617 | 0.99 |
| 74_V | 100_C | 1.614 | 0.99 |
| 174_E | 318_T | 1.612 | 0.99 |
| 223_H | 402_V | 1.608 | 0.99 |
| 119_Q | 408_K | 1.607 | 0.99 |
| 103_I | 114_F | 1.607 | 0.99 |
| 179_S | 190_S | 1.605 | 0.99 |
| 333_M | 337_I | 1.605 | 0.99 |
| 242_I | 436_C | 1.590 | 0.99 |
| 105_A | 108_Q | 1.584 | 0.99 |
| 176_P | 179_S | 1.547 | 0.98 |
| 66_Q | 92_Y | 1.544 | 0.98 |
| 86_A | 89_G | 1.542 | 0.98 |
| 335_R | 339_A | 1.528 | 0.98 |
| 208_D | 416_I | 1.528 | 0.98 |
| 244_S | 291_L | 1.521 | 0.98 |
| 91_R | 94_F | 1.519 | 0.98 |
| 216_L | 404_F | 1.512 | 0.98 |
| 175_F | 181_L | 1.511 | 0.98 |
| 114_F | 135_L | 1.510 | 0.98 |
| 211_E | 412_V | 1.504 | 0.98 |
| 71_V | 129_S | 1.484 | 0.98 |
| 215_A | 409_F | 1.473 | 0.98 |
| 156_L | 183_S | 1.472 | 0.98 |
| 5_F | 47_A | 1.470 | 0.98 |
| 94_F | 102_V | 1.469 | 0.98 |
| 356_G | 422_M | 1.466 | 0.98 |
| 219_S | 223_H | 1.459 | 0.97 |
| 209_K | 436_C | 1.452 | 0.97 |
| 315_H | 395_A | 1.447 | 0.97 |
| 304_E | 307_D | 1.444 | 0.97 |
| 107_Y | 114_F | 1.444 | 0.97 |
| 412_V | 416_I | 1.444 | 0.97 |
| 81_H | 85_R | 1.439 | 0.97 |
| 326_A | 388_R | 1.429 | 0.97 |
| 70_H | 108_Q | 1.427 | 0.97 |
| 126_L | 135_L | 1.426 | 0.97 |
| 409_F | 413_A | 1.424 | 0.97 |
| 69_T | 130_E | 1.423 | 0.97 |
| 6_G | 136_I | 1.422 | 0.97 |
| 179_S | 188_I | 1.421 | 0.97 |
| 232_V | 247_T | 1.418 | 0.97 |
| 135_L | 180_Y | 1.415 | 0.97 |
| 301_T | 304_E | 1.397 | 0.96 |
| 124_F | 137_G | 1.396 | 0.96 |
| 103_I | 118_L | 1.390 | 0.96 |
| 230_Y | 344_M | 1.386 | 0.96 |
| 92_Y | 108_Q | 1.384 | 0.96 |
| 81_H | 225_M | 1.381 | 0.96 |
| 30_H | 161_E | 1.375 | 0.96 |
| 78_I | 103_I | 1.358 | 0.96 |
| 218_D | 222_S | 1.354 | 0.96 |
| 35_W | 48_H | 1.350 | 0.95 |
| 63_L | 104_L | 1.350 | 0.95 |
| 347_S | 400_A | 1.349 | 0.95 |
| 157_Y | 165_L | 1.347 | 0.95 |
| 66_Q | 105_A | 1.341 | 0.95 |
| 169_C | 173_K | 1.339 | 0.95 |
| 215_A | 408_K | 1.333 | 0.95 |
| 336_K | 339_A | 1.328 | 0.95 |
| 65_N | 68_K | 1.323 | 0.95 |
| 349_E | 389_A | 1.321 | 0.95 |
| 212_L | 413_A | 1.318 | 0.95 |
| 65_N | 70_H | 1.315 | 0.95 |
| 389_A | 400_A | 1.315 | 0.95 |
| 29_R | 49_E | 1.304 | 0.94 |
| 438_E | 443_A | 1.294 | 0.94 |
| 316_I | 392_A | 1.288 | 0.94 |
| 106_L | 117_D | 1.288 | 0.94 |
| 176_P | 188_I | 1.287 | 0.94 |
| 28_M | 49_E | 1.283 | 0.94 |
| 211_E | 214_Q | 1.283 | 0.94 |
| 216_L | 243_I | 1.275 | 0.93 |
| 244_S | 270_S | 1.273 | 0.93 |
| 137_G | 180_Y | 1.272 | 0.93 |
| 222_S | 225_M | 1.268 | 0.93 |
| 330_M | 349_E | 1.267 | 0.93 |
| 127_Y | 132_D | 1.261 | 0.93 |
| 29_R | 397_G | 1.255 | 0.93 |
| 241_S | 441_L | 1.250 | 0.92 |
| 477_Q | 500_E | 1.249 | 0.92 |
| 436_C | 439_A | 1.249 | 0.92 |
| 112_P | 189_R | 1.247 | 0.92 |
| 230_Y | 343_K | 1.247 | 0.92 |
| 337_I | 345_V | 1.244 | 0.92 |
| 209_K | 435_E | 1.240 | 0.92 |
| 147_Y | 391_K | 1.239 | 0.92 |
| 205_N | 435_E | 1.239 | 0.92 |
| 334_S | 393_M | 1.229 | 0.91 |
| 281_L | 297_E | 1.217 | 0.91 |
| 87_E | 110_K | 1.214 | 0.91 |
| 25_S | 49_E | 1.214 | 0.91 |
| 283_A | 448_R | 1.210 | 0.91 |
| 54_V | 226_S | 1.206 | 0.90 |
| 72_L | 126_L | 1.198 | 0.90 |
| 205_N | 431_H | 1.196 | 0.90 |
| 247_T | 268_L | 1.190 | 0.90 |
| 69_T | 108_Q | 1.187 | 0.90 |
| 408_K | 411_D | 1.187 | 0.90 |
| 28_M | 164_A | 1.184 | 0.89 |
| 212_L | 409_F | 1.183 | 0.89 |
| 232_V | 270_S | 1.182 | 0.89 |
| 65_N | 92_Y | 1.179 | 0.89 |
| 331_Y | 396_W | 1.176 | 0.89 |
| 105_A | 109_E | 1.171 | 0.89 |
| 2_C | 75_N | 1.165 | 0.88 |
| 114_F | 124_F | 1.160 | 0.88 |
| 163_K | 314_Y | 1.158 | 0.88 |
| 22_L | 36_S | 1.158 | 0.88 |
| 40_A | 45_I | 1.157 | 0.88 |
| 121_M | 144_I | 1.141 | 0.87 |
| 310_R | 504_E | 1.138 | 0.87 |
| 274_G | 281_L | 1.137 | 0.87 |
| 53_I | 344_M | 1.135 | 0.87 |
| 27_L | 167_P | 1.134 | 0.86 |
| 41_S | 129_S | 1.126 | 0.86 |
| 140_H | 223_H | 1.125 | 0.86 |
| 322_T | 349_E | 1.123 | 0.86 |
| 112_P | 135_L | 1.122 | 0.86 |
| 404_F | 433_L | 1.119 | 0.85 |
| 27_L | 314_Y | 1.115 | 0.85 |
| 218_D | 221_K | 1.114 | 0.85 |
| 113_E | 116_D | 1.114 | 0.85 |
| 80_N | 84_L | 1.113 | 0.85 |
| 322_T | 326_A | 1.109 | 0.85 |
| 241_S | 283_A | 1.109 | 0.85 |
| 133_A | 182_W | 1.108 | 0.85 |
| 226_S | 230_Y | 1.107 | 0.85 |
| 128_D | 131_K | 1.103 | 0.84 |
| 246_I | 440_Y | 1.102 | 0.84 |
| 8_F | 134_Y | 1.101 | 0.84 |
| 69_T | 129_S | 1.100 | 0.84 |
| 69_T | 131_K | 1.100 | 0.84 |
| 6_G | 158_V | 1.099 | 0.84 |
| 115_L | 135_L | 1.098 | 0.84 |
| 6_G | 125_A | 1.098 | 0.84 |
| 308_A | 332_L | 1.096 | 0.84 |
| 330_M | 388_R | 1.093 | 0.84 |
| 127_Y | 134_Y | 1.089 | 0.83 |
| 4_I | 73_A | 1.089 | 0.83 |
| 46_L | 73_A | 1.087 | 0.83 |
| 329_P | 388_R | 1.081 | 0.83 |
| 72_L | 104_L | 1.076 | 0.82 |
| 233_L | 337_I | 1.075 | 0.82 |
| 216_L | 433_L | 1.069 | 0.82 |
| 276_P | 299_H | 1.067 | 0.82 |
| 107_Y | 131_K | 1.064 | 0.81 |
| 217_E | 250_Y | 1.058 | 0.81 |
| 144_I | 394_S | 1.057 | 0.81 |
| 81_H | 102_V | 1.056 | 0.81 |
| 324_I | 330_M | 1.052 | 0.80 |
| 434_R | 447_W | 1.051 | 0.80 |
| 213_R | 217_E | 1.050 | 0.80 |
| 210_N | 439_A | 1.049 | 0.80 |
| 66_Q | 108_Q | 1.042 | 0.79 |
| 5_F | 25_S | 1.039 | 0.79 |
| 64_Y | 68_K | 1.036 | 0.79 |
| 175_F | 179_S | 1.033 | 0.79 |
| 82_Q | 97_G | 1.029 | 0.78 |
| 22_L | 38_I | 1.028 | 0.78 |
| 208_D | 420_D | 1.027 | 0.78 |
| 272_A | 284_A | 1.022 | 0.78 |
| 16_E | 45_I | 1.015 | 0.77 |
| 280_D | 385_D | 1.012 | 0.77 |
| 85_R | 94_F | 1.011 | 0.76 |
| 136_I | 156_L | 1.007 | 0.76 |
| 148_M | 188_I | 1.002 | 0.76 |
| 25_S | 28_M | 1.002 | 0.76 |
| 287_V | 445_V | 1.001 | 0.75 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1ct9A | 4 | 0.9061 | 100 | 0.199 | Contact Map |
| 1q15A | 4 | 0.8195 | 100 | 0.402 | Contact Map |
| 1jgtA | 2 | 0.8213 | 100 | 0.437 | Contact Map |
| 1ao0A | 4 | 0.7022 | 100 | 0.732 | Contact Map |
| 1xffA | 2 | 0.3556 | 100 | 0.753 | Contact Map |
| 1ecfA | 2 | 0.7202 | 100 | 0.762 | Contact Map |
| 4amvA | 3 | 0.8899 | 100 | 0.764 | Contact Map |
| 1te5A | 2 | 0.3159 | 100 | 0.765 | Contact Map |
| 3mdnA | 4 | 0.2978 | 100 | 0.798 | Contact Map |
| 4zflA | 12 | 0 | 100 | 0.807 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)