| ATC |
| ID: | 1497295511 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 665 (631) | ||
| Sequences: | 17856 (14181.9) | ||
| Seq/Len: | 28.298 | ||
| Nf(neff/√len): | 564.6 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 28.298).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 129_T | 146_I | 4.448 | 1.00 |
| 327_L | 365_G | 3.849 | 1.00 |
| 402_Y | 440_H | 3.556 | 1.00 |
| 52_R | 78_V | 3.410 | 1.00 |
| 148_T | 336_Q | 3.192 | 1.00 |
| 95_N | 134_Y | 2.898 | 1.00 |
| 50_G | 128_D | 2.870 | 1.00 |
| 50_G | 336_Q | 2.855 | 1.00 |
| 80_I | 89_Q | 2.735 | 1.00 |
| 540_D | 543_S | 2.690 | 1.00 |
| 32_D | 72_K | 2.683 | 1.00 |
| 312_L | 328_Y | 2.610 | 1.00 |
| 366_R | 374_E | 2.541 | 1.00 |
| 36_N | 72_K | 2.512 | 1.00 |
| 502_E | 531_R | 2.440 | 1.00 |
| 512_N | 551_R | 2.429 | 1.00 |
| 492_L | 558_K | 2.420 | 1.00 |
| 269_D | 287_P | 2.393 | 1.00 |
| 341_D | 344_K | 2.366 | 1.00 |
| 54_G | 98_C | 2.365 | 1.00 |
| 170_R | 195_T | 2.336 | 1.00 |
| 7_I | 32_D | 2.334 | 1.00 |
| 51_D | 99_K | 2.302 | 1.00 |
| 41_F | 45_R | 2.278 | 1.00 |
| 515_F | 520_G | 2.257 | 1.00 |
| 284_I | 309_Y | 2.220 | 1.00 |
| 9_Q | 13_T | 2.200 | 1.00 |
| 324_I | 376_L | 2.142 | 1.00 |
| 38_V | 65_I | 2.114 | 1.00 |
| 311_V | 365_G | 2.112 | 1.00 |
| 514_H | 548_P | 2.112 | 1.00 |
| 148_T | 151_A | 2.086 | 1.00 |
| 47_I | 75_A | 2.072 | 1.00 |
| 29_S | 33_Q | 2.064 | 1.00 |
| 210_R | 214_R | 2.063 | 1.00 |
| 499_V | 528_V | 2.061 | 1.00 |
| 151_A | 280_S | 2.058 | 1.00 |
| 32_D | 119_D | 2.043 | 1.00 |
| 549_V | 552_D | 2.040 | 1.00 |
| 330_G | 358_I | 2.007 | 1.00 |
| 169_D | 218_T | 1.987 | 1.00 |
| 496_L | 532_L | 1.978 | 1.00 |
| 514_H | 517_A | 1.966 | 1.00 |
| 49_R | 128_D | 1.956 | 1.00 |
| 38_V | 69_A | 1.955 | 1.00 |
| 486_V | 512_N | 1.954 | 1.00 |
| 91_Y | 141_R | 1.945 | 1.00 |
| 511_A | 550_L | 1.945 | 1.00 |
| 13_T | 16_Q | 1.937 | 1.00 |
| 9_Q | 12_R | 1.919 | 1.00 |
| 52_R | 132_V | 1.913 | 1.00 |
| 47_I | 51_D | 1.904 | 1.00 |
| 368_R | 374_E | 1.904 | 1.00 |
| 326_D | 360_K | 1.895 | 1.00 |
| 324_I | 364_C | 1.892 | 1.00 |
| 427_V | 460_L | 1.880 | 1.00 |
| 36_N | 40_R | 1.878 | 1.00 |
| 489_Q | 511_A | 1.871 | 1.00 |
| 490_A | 558_K | 1.857 | 1.00 |
| 129_T | 336_Q | 1.852 | 1.00 |
| 309_Y | 329_I | 1.847 | 1.00 |
| 510_G | 513_D | 1.844 | 1.00 |
| 151_A | 334_L | 1.842 | 1.00 |
| 500_W | 550_L | 1.832 | 1.00 |
| 396_S | 410_V | 1.832 | 1.00 |
| 132_V | 339_L | 1.831 | 1.00 |
| 491_G | 494_K | 1.824 | 1.00 |
| 426_V | 454_L | 1.817 | 1.00 |
| 313_N | 317_K | 1.811 | 1.00 |
| 17_V | 25_W | 1.792 | 1.00 |
| 308_T | 330_G | 1.791 | 1.00 |
| 63_T | 177_M | 1.788 | 1.00 |
| 424_G | 454_L | 1.776 | 1.00 |
| 425_Y | 474_P | 1.769 | 1.00 |
| 385_I | 412_D | 1.767 | 1.00 |
| 383_V | 392_L | 1.764 | 1.00 |
| 154_N | 306_N | 1.753 | 1.00 |
| 381_Y | 470_R | 1.747 | 1.00 |
| 169_D | 244_R | 1.742 | 1.00 |
| 125_E | 128_D | 1.739 | 1.00 |
| 400_L | 407_Q | 1.730 | 1.00 |
| 161_R | 305_A | 1.726 | 1.00 |
| 51_D | 75_A | 1.722 | 1.00 |
| 15_D | 29_S | 1.717 | 1.00 |
| 225_A | 251_D | 1.715 | 1.00 |
| 543_S | 556_T | 1.713 | 1.00 |
| 368_R | 372_Y | 1.710 | 1.00 |
| 200_R | 203_R | 1.709 | 1.00 |
| 35_S | 72_K | 1.706 | 1.00 |
| 547_F | 556_T | 1.683 | 1.00 |
| 320_P | 323_V | 1.666 | 1.00 |
| 533_F | 538_H | 1.659 | 1.00 |
| 311_V | 373_I | 1.659 | 1.00 |
| 426_V | 456_H | 1.657 | 1.00 |
| 20_V | 195_T | 1.646 | 1.00 |
| 401_T | 444_H | 1.636 | 1.00 |
| 493_E | 511_A | 1.627 | 1.00 |
| 48_G | 51_D | 1.626 | 1.00 |
| 69_A | 73_A | 1.625 | 1.00 |
| 286_W | 299_P | 1.618 | 1.00 |
| 414_T | 420_K | 1.611 | 1.00 |
| 503_V | 528_V | 1.608 | 1.00 |
| 52_R | 76_A | 1.606 | 1.00 |
| 127_E | 150_Q | 1.595 | 1.00 |
| 197_V | 217_I | 1.577 | 1.00 |
| 495_I | 535_Q | 1.576 | 1.00 |
| 352_H | 356_G | 1.574 | 1.00 |
| 521_D | 524_K | 1.574 | 1.00 |
| 21_F | 61_V | 1.572 | 1.00 |
| 93_L | 98_C | 1.567 | 1.00 |
| 309_Y | 327_L | 1.564 | 1.00 |
| 32_D | 35_S | 1.555 | 1.00 |
| 40_R | 120_L | 1.545 | 1.00 |
| 32_D | 36_N | 1.542 | 1.00 |
| 34_R | 62_E | 1.535 | 1.00 |
| 55_V | 66_N | 1.532 | 1.00 |
| 499_V | 532_L | 1.529 | 1.00 |
| 490_A | 511_A | 1.527 | 1.00 |
| 655_Q | 659_D | 1.526 | 1.00 |
| 457_L | 460_L | 1.519 | 1.00 |
| 66_N | 102_L | 1.518 | 1.00 |
| 180_D | 276_A | 1.516 | 1.00 |
| 26_L | 65_I | 1.516 | 1.00 |
| 8_D | 12_R | 1.514 | 1.00 |
| 497_A | 550_L | 1.512 | 1.00 |
| 308_T | 352_H | 1.511 | 1.00 |
| 169_D | 194_A | 1.510 | 1.00 |
| 170_R | 217_I | 1.506 | 1.00 |
| 500_W | 520_G | 1.499 | 1.00 |
| 221_N | 282_W | 1.498 | 1.00 |
| 490_A | 494_K | 1.496 | 1.00 |
| 95_N | 341_D | 1.492 | 1.00 |
| 516_F | 545_F | 1.484 | 1.00 |
| 286_W | 372_Y | 1.481 | 1.00 |
| 1_T | 4_Q | 1.477 | 1.00 |
| 400_L | 408_A | 1.474 | 1.00 |
| 398_C | 441_L | 1.470 | 1.00 |
| 20_V | 215_R | 1.468 | 1.00 |
| 7_I | 72_K | 1.466 | 1.00 |
| 127_E | 148_T | 1.460 | 1.00 |
| 211_T | 215_R | 1.441 | 1.00 |
| 342_D | 346_K | 1.439 | 1.00 |
| 388_Y | 448_Y | 1.429 | 1.00 |
| 409_V | 460_L | 1.428 | 1.00 |
| 485_F | 508_Q | 1.427 | 1.00 |
| 423_V | 455_V | 1.424 | 1.00 |
| 104_S | 107_Y | 1.423 | 1.00 |
| 204_D | 207_E | 1.418 | 1.00 |
| 321_V | 367_M | 1.406 | 0.99 |
| 91_Y | 134_Y | 1.406 | 0.99 |
| 197_V | 211_T | 1.403 | 0.99 |
| 399_L | 424_G | 1.403 | 0.99 |
| 486_V | 511_A | 1.400 | 0.99 |
| 283_S | 329_I | 1.392 | 0.99 |
| 425_Y | 476_P | 1.392 | 0.99 |
| 6_F | 10_A | 1.391 | 0.99 |
| 181_L | 221_N | 1.389 | 0.99 |
| 17_V | 195_T | 1.378 | 0.99 |
| 184_Y | 282_W | 1.375 | 0.99 |
| 90_T | 94_E | 1.372 | 0.99 |
| 492_L | 495_I | 1.371 | 0.99 |
| 78_V | 96_S | 1.366 | 0.99 |
| 199_I | 211_T | 1.364 | 0.99 |
| 309_Y | 365_G | 1.363 | 0.99 |
| 344_K | 348_A | 1.361 | 0.99 |
| 160_N | 192_A | 1.357 | 0.99 |
| 95_N | 141_R | 1.356 | 0.99 |
| 86_Y | 90_T | 1.352 | 0.99 |
| 17_V | 20_V | 1.343 | 0.99 |
| 414_T | 417_R | 1.340 | 0.99 |
| 364_C | 379_Q | 1.329 | 0.99 |
| 273_L | 282_W | 1.329 | 0.99 |
| 497_A | 511_A | 1.327 | 0.99 |
| 3_H | 149_H | 1.321 | 0.99 |
| 489_Q | 551_R | 1.318 | 0.99 |
| 412_D | 420_K | 1.317 | 0.99 |
| 169_D | 219_I | 1.316 | 0.99 |
| 496_L | 554_A | 1.314 | 0.99 |
| 174_I | 223_V | 1.312 | 0.99 |
| 316_Q | 328_Y | 1.309 | 0.99 |
| 26_L | 61_V | 1.308 | 0.99 |
| 175_S | 181_L | 1.307 | 0.99 |
| 2_L | 152_A | 1.300 | 0.99 |
| 528_V | 544_L | 1.298 | 0.99 |
| 350_I | 358_I | 1.294 | 0.99 |
| 203_R | 207_E | 1.290 | 0.99 |
| 495_I | 498_S | 1.289 | 0.99 |
| 328_Y | 360_K | 1.289 | 0.99 |
| 124_A | 128_D | 1.288 | 0.99 |
| 410_V | 424_G | 1.287 | 0.99 |
| 6_F | 68_M | 1.284 | 0.99 |
| 352_H | 358_I | 1.284 | 0.99 |
| 84_H | 92_I | 1.269 | 0.99 |
| 170_R | 197_V | 1.269 | 0.99 |
| 616_F | 619_V | 1.259 | 0.99 |
| 494_K | 498_S | 1.258 | 0.99 |
| 133_I | 277_T | 1.257 | 0.99 |
| 511_A | 551_R | 1.253 | 0.99 |
| 155_T | 184_Y | 1.253 | 0.99 |
| 155_T | 282_W | 1.249 | 0.99 |
| 47_I | 53_V | 1.246 | 0.98 |
| 619_V | 623_H | 1.245 | 0.98 |
| 7_I | 35_S | 1.244 | 0.98 |
| 496_L | 550_L | 1.239 | 0.98 |
| 172_I | 199_I | 1.236 | 0.98 |
| 94_E | 141_R | 1.233 | 0.98 |
| 55_V | 67_L | 1.233 | 0.98 |
| 57_A | 66_N | 1.230 | 0.98 |
| 527_Q | 531_R | 1.229 | 0.98 |
| 84_H | 178_C | 1.226 | 0.98 |
| 184_Y | 221_N | 1.226 | 0.98 |
| 537_Y | 557_I | 1.225 | 0.98 |
| 382_Q | 391_E | 1.222 | 0.98 |
| 27_T | 30_E | 1.222 | 0.98 |
| 88_R | 394_E | 1.222 | 0.98 |
| 146_I | 339_L | 1.219 | 0.98 |
| 254_P | 257_L | 1.219 | 0.98 |
| 5_L | 153_S | 1.217 | 0.98 |
| 543_S | 546_E | 1.217 | 0.98 |
| 221_N | 273_L | 1.216 | 0.98 |
| 21_F | 24_E | 1.216 | 0.98 |
| 398_C | 444_H | 1.215 | 0.98 |
| 542_K | 546_E | 1.214 | 0.98 |
| 64_I | 186_I | 1.213 | 0.98 |
| 498_S | 535_Q | 1.211 | 0.98 |
| 438_R | 442_M | 1.209 | 0.98 |
| 62_E | 104_S | 1.206 | 0.98 |
| 433_D | 436_A | 1.205 | 0.98 |
| 166_N | 169_D | 1.204 | 0.98 |
| 185_D | 221_N | 1.203 | 0.98 |
| 279_A | 329_I | 1.199 | 0.98 |
| 76_A | 129_T | 1.198 | 0.98 |
| 489_Q | 493_E | 1.197 | 0.98 |
| 335_A | 349_F | 1.195 | 0.98 |
| 66_N | 70_V | 1.195 | 0.98 |
| 96_S | 134_Y | 1.194 | 0.98 |
| 414_T | 418_G | 1.192 | 0.98 |
| 255_L | 259_A | 1.192 | 0.98 |
| 19_V | 61_V | 1.187 | 0.98 |
| 529_S | 544_L | 1.186 | 0.98 |
| 438_R | 454_L | 1.185 | 0.98 |
| 247_L | 273_L | 1.183 | 0.98 |
| 532_L | 537_Y | 1.183 | 0.98 |
| 147_I | 335_A | 1.177 | 0.98 |
| 35_S | 69_A | 1.176 | 0.98 |
| 311_V | 327_L | 1.176 | 0.98 |
| 58_K | 104_S | 1.167 | 0.97 |
| 310_Y | 354_E | 1.165 | 0.97 |
| 500_W | 553_V | 1.161 | 0.97 |
| 440_H | 444_H | 1.161 | 0.97 |
| 428_A | 456_H | 1.152 | 0.97 |
| 245_L | 271_I | 1.150 | 0.97 |
| 409_V | 468_L | 1.148 | 0.97 |
| 525_A | 544_L | 1.147 | 0.97 |
| 304_L | 307_Q | 1.146 | 0.97 |
| 313_N | 319_C | 1.143 | 0.97 |
| 525_A | 529_S | 1.141 | 0.97 |
| 86_Y | 110_M | 1.137 | 0.97 |
| 310_Y | 358_I | 1.135 | 0.97 |
| 145_V | 335_A | 1.134 | 0.97 |
| 310_Y | 352_H | 1.134 | 0.97 |
| 155_T | 280_S | 1.133 | 0.97 |
| 425_Y | 473_L | 1.133 | 0.97 |
| 302_K | 372_Y | 1.133 | 0.97 |
| 20_V | 25_W | 1.130 | 0.97 |
| 3_H | 124_A | 1.122 | 0.97 |
| 249_S | 282_W | 1.121 | 0.97 |
| 134_Y | 144_G | 1.117 | 0.96 |
| 365_G | 375_F | 1.117 | 0.96 |
| 181_L | 249_S | 1.116 | 0.96 |
| 328_Y | 350_I | 1.112 | 0.96 |
| 228_D | 257_L | 1.112 | 0.96 |
| 646_D | 649_L | 1.112 | 0.96 |
| 221_N | 224_P | 1.111 | 0.96 |
| 189_T | 196_L | 1.109 | 0.96 |
| 131_Y | 147_I | 1.108 | 0.96 |
| 91_Y | 95_N | 1.106 | 0.96 |
| 617_Q | 620_I | 1.105 | 0.96 |
| 47_I | 73_A | 1.105 | 0.96 |
| 6_F | 32_D | 1.103 | 0.96 |
| 398_C | 401_T | 1.101 | 0.96 |
| 221_N | 249_S | 1.096 | 0.96 |
| 45_R | 100_I | 1.094 | 0.96 |
| 5_L | 157_Q | 1.093 | 0.96 |
| 488_P | 511_A | 1.093 | 0.96 |
| 71_L | 130_A | 1.091 | 0.96 |
| 155_T | 281_I | 1.089 | 0.96 |
| 496_L | 553_V | 1.088 | 0.96 |
| 54_G | 80_I | 1.087 | 0.96 |
| 156_I | 191_S | 1.087 | 0.96 |
| 392_L | 410_V | 1.086 | 0.96 |
| 54_G | 93_L | 1.086 | 0.96 |
| 17_V | 27_T | 1.086 | 0.96 |
| 485_F | 510_G | 1.082 | 0.96 |
| 396_S | 470_R | 1.080 | 0.95 |
| 220_W | 243_L | 1.078 | 0.95 |
| 19_V | 64_I | 1.076 | 0.95 |
| 497_A | 509_I | 1.072 | 0.95 |
| 366_R | 376_L | 1.071 | 0.95 |
| 10_A | 32_D | 1.071 | 0.95 |
| 76_A | 132_V | 1.071 | 0.95 |
| 216_G | 242_S | 1.071 | 0.95 |
| 150_Q | 332_A | 1.069 | 0.95 |
| 300_Y | 375_F | 1.063 | 0.95 |
| 56_L | 89_Q | 1.062 | 0.95 |
| 497_A | 501_Q | 1.058 | 0.95 |
| 77_Y | 183_V | 1.058 | 0.95 |
| 24_E | 34_R | 1.057 | 0.95 |
| 181_L | 282_W | 1.055 | 0.95 |
| 651_I | 655_Q | 1.054 | 0.95 |
| 165_V | 194_A | 1.053 | 0.95 |
| 532_L | 557_I | 1.052 | 0.95 |
| 103_D | 107_Y | 1.041 | 0.94 |
| 529_S | 533_F | 1.040 | 0.94 |
| 22_E | 200_R | 1.038 | 0.94 |
| 531_R | 535_Q | 1.038 | 0.94 |
| 406_D | 429_Q | 1.037 | 0.94 |
| 523_I | 526_I | 1.036 | 0.94 |
| 490_A | 493_E | 1.036 | 0.94 |
| 492_L | 557_I | 1.029 | 0.94 |
| 385_I | 390_V | 1.021 | 0.93 |
| 393_E | 396_S | 1.020 | 0.93 |
| 50_G | 129_T | 1.020 | 0.93 |
| 328_Y | 358_I | 1.020 | 0.93 |
| 184_Y | 273_L | 1.018 | 0.93 |
| 189_T | 194_A | 1.018 | 0.93 |
| 493_E | 554_A | 1.017 | 0.93 |
| 41_F | 44_S | 1.017 | 0.93 |
| 172_I | 212_V | 1.014 | 0.93 |
| 528_V | 553_V | 1.012 | 0.93 |
| 547_F | 552_D | 1.012 | 0.93 |
| 173_G | 185_D | 1.010 | 0.93 |
| 501_Q | 508_Q | 1.009 | 0.93 |
| 310_Y | 330_G | 1.008 | 0.93 |
| 299_P | 372_Y | 1.007 | 0.93 |
| 486_V | 551_R | 1.006 | 0.93 |
| 279_A | 283_S | 1.003 | 0.93 |
| 156_I | 188_G | 1.001 | 0.92 |
| 155_T | 185_D | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2vsqA | 1 | 0.9444 | 100 | 0.131 | Contact Map |
| 4dg8A | 1 | 0.7053 | 100 | 0.167 | Contact Map |
| 4iz6A | 2 | 0.8271 | 100 | 0.192 | Contact Map |
| 4r0mB | 2 | 0.8241 | 100 | 0.225 | Contact Map |
| 4d4iA | 1 | 0 | 100 | 0.291 | Contact Map |
| 1amuA | 2 | 0.7023 | 100 | 0.292 | Contact Map |
| 3g7sA | 2 | 0.6556 | 100 | 0.321 | Contact Map |
| 3wvnA | 2 | 0.5398 | 100 | 0.326 | Contact Map |
| 3tsyA | 1 | 0.7699 | 100 | 0.33 | Contact Map |
| 3vnrA | 1 | 0.6977 | 100 | 0.336 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)