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OPENSEQ.org

FZD6_med

ID: 1494603467 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 50% gaps before running GREMLIN.
Length: 574 (514)
Sequences: 726 (496.6)
Seq/Len: 1.412
Nf(neff/√len): 21.9

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.412).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 50% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
230_C234_C2.7991.00
392_K432_Y2.4951.00
403_D414_G2.2890.99
256_W260_W2.2490.99
121_R125_E2.1920.99
62_Y65_T2.1030.99
49_C62_Y2.0280.98
265_C297_F2.0190.98
210_C214_L2.0140.98
54_I107_A1.8670.96
94_C103_C1.8190.95
369_W372_E1.7740.95
210_C213_Q1.7560.94
385_A389_P1.7400.94
49_C57_C1.7050.93
125_E128_R1.6950.93
103_C110_C1.6860.92
403_D415_L1.6750.92
336_C411_C1.6500.91
131_C148_C1.6440.91
281_R284_Y1.6340.91
544_T549_W1.6190.90
422_R493_Q1.6190.90
59_D99_R1.6150.90
382_H386_W1.5890.89
124_C131_C1.5890.89
57_C62_Y1.5860.89
55_P84_H1.5750.88
268_T290_I1.5680.88
97_E126_R1.5620.88
50_Q76_Q1.5140.86
118_P159_I1.5060.85
110_C124_C1.4910.84
252_F260_W1.4890.84
343_L410_V1.4780.84
63_N73_H1.4720.83
386_W389_P1.4670.83
159_I163_Q1.4530.82
282_F287_R1.4440.82
196_G199_D1.4370.81
80_G85_Q1.4350.81
109_V161_V1.4270.81
264_C281_R1.4180.80
451_R464_L1.4140.80
365_A372_E1.4110.80
301_V304_V1.4060.79
94_C110_C1.4040.79
98_L102_L1.3990.79
492_E496_R1.3900.78
103_C124_C1.3830.78
102_L106_Y1.3770.77
340_F344_Y1.3420.75
274_T284_Y1.3410.75
80_G84_H1.3390.75
354_W361_W1.3320.74
545_G549_W1.3300.74
274_T281_R1.3230.73
198_S202_G1.3190.73
295_C349_A1.3180.73
444_F545_G1.3080.72
132_E136_N1.2930.71
370_G373_A1.2910.71
284_Y295_C1.2880.70
109_V138_F1.2880.70
302_A306_G1.2880.70
282_F286_E1.2850.70
345_F393_T1.2830.70
414_G421_L1.2820.70
538_M552_K1.2810.70
496_R500_E1.2770.69
548_I553_T1.2750.69
437_T448_F1.2740.69
126_R130_G1.2730.69
396_I413_V1.2690.69
106_Y140_F1.2650.68
50_Q54_I1.2630.68
252_F256_W1.2620.68
284_Y291_F1.2600.68
422_R427_A1.2520.67
464_L481_P1.2520.67
127_A135_M1.2510.67
285_P386_W1.2460.67
525_P530_F1.2400.66
363_L369_W1.2390.66
353_W386_W1.2390.66
81_L84_H1.2320.65
364_A373_A1.2300.65
49_C52_I1.2300.65
341_M345_F1.2290.65
264_C284_Y1.2280.65
124_C148_C1.2240.65
550_S553_T1.2240.65
261_S268_T1.2210.64
414_G417_S1.2190.64
548_I554_L1.2110.64
491_Y499_W1.2100.63
532_I537_T1.2030.63
302_A305_A1.2020.63
554_L558_R1.2020.63
538_M550_S1.2010.63
67_L76_Q1.2010.63
282_F290_I1.1960.62
135_M140_F1.1920.62
275_Y284_Y1.1910.62
305_A309_L1.1890.61
100_F161_V1.1880.61
287_R295_C1.1830.61
65_T76_Q1.1800.61
365_A368_K1.1790.60
412_Y425_V1.1780.60
354_W436_G1.1780.60
464_L468_M1.1750.60
531_M549_W1.1710.60
73_H81_L1.1700.60
110_C131_C1.1690.59
445_V476_V1.1680.59
285_P291_F1.1660.59
177_T180_P1.1640.59
285_P382_H1.1640.59
282_F288_P1.1640.59
209_S215_K1.1630.59
268_T297_F1.1620.59
272_V297_F1.1610.59
268_T352_I1.1590.58
348_M392_K1.1590.58
80_G83_V1.1580.58
50_Q53_S1.1570.58
264_C271_T1.1570.58
281_R285_P1.1480.57
436_G481_P1.1450.57
499_W525_P1.1420.57
73_H79_A1.1410.57
103_C131_C1.1390.56
384_A427_A1.1380.56
204_P208_F1.1370.56
353_W361_W1.1340.56
131_C143_P1.1310.56
234_C315_C1.1310.56
553_T557_W1.1310.56
471_I548_I1.1300.56
211_P222_Y1.1300.56
278_D287_R1.1290.55
461_T464_L1.1280.55
57_C65_T1.1280.55
284_Y287_R1.1270.55
90_V105_M1.1260.55
315_C318_R1.1250.55
196_G203_R1.1240.55
119_P132_E1.1240.55
89_L102_L1.1220.55
275_Y287_R1.1200.55
278_D284_Y1.1180.54
411_C428_P1.1170.54
313_A316_V1.1160.54
357_L361_W1.1160.54
73_H90_V1.1150.54
281_R287_R1.1140.54
470_R534_Y1.1140.54
120_C124_C1.1110.54
556_S560_F1.1100.54
85_Q90_V1.1080.53
68_P73_H1.1080.53
528_T546_F1.1070.53
471_I474_F1.1060.53
458_G461_T1.1040.53
372_E382_H1.0970.52
97_E130_G1.0950.52
392_K423_G1.0940.52
45_D75_N1.0930.52
62_Y69_N1.0920.52
348_M432_Y1.0920.52
158_E163_Q1.0910.52
127_A130_G1.0880.51
256_W264_C1.0880.51
94_C124_C1.0860.51
117_I126_R1.0840.51
86_F90_V1.0830.51
405_D409_G1.0830.51
361_W382_H1.0820.51
282_F285_P1.0800.51
383_L387_A1.0780.50
85_Q97_E1.0780.50
84_H92_V1.0760.50
104_S125_E1.0750.50
422_R426_L1.0750.50
148_C160_C1.0740.50
47_G67_L1.0730.50
531_M544_T1.0730.50
125_E129_Q1.0730.50
450_I548_I1.0720.50
65_T69_N1.0720.50
278_D285_P1.0720.50
68_P79_A1.0710.50
360_T385_A1.0710.50
155_G159_I1.0700.50
344_Y405_D1.0700.50
198_S201_R1.0700.50
109_V123_L1.0700.50
255_L258_G1.0650.49
294_G298_M1.0650.49
436_G464_L1.0650.49
354_W481_P1.0640.49
67_L70_L1.0630.49
488_C492_E1.0620.49
272_V276_L1.0620.49
353_W382_H1.0610.49
77_E83_V1.0610.49
63_N68_P1.0600.48
474_F478_Y1.0590.48
95_S120_C1.0590.48
229_D233_P1.0590.48
535_L539_I1.0570.48
355_V360_T1.0550.48
123_L138_F1.0540.48
285_P289_I1.0540.48
471_I475_S1.0530.48
281_R288_P1.0530.48
58_T86_F1.0510.48
274_T278_D1.0500.47
196_G201_R1.0490.47
424_F433_L1.0490.47
330_G387_A1.0480.47
541_G544_T1.0470.47
298_M301_V1.0440.47
52_I76_Q1.0430.47
117_I159_I1.0410.47
56_L90_V1.0390.46
403_D416_S1.0380.46
94_C131_C1.0370.46
368_K381_F1.0360.46
365_A369_W1.0350.46
105_M146_L1.0340.46
526_D538_M1.0330.46
200_G205_A1.0330.46
143_P148_C1.0320.46
49_C65_T1.0290.45
54_I63_N1.0280.45
170_G175_G1.0280.45
204_P207_P1.0280.45
208_F213_Q1.0280.45
196_G202_G1.0250.45
55_P60_I1.0220.45
282_F538_M1.0220.45
492_E533_K1.0180.44
275_Y285_P1.0180.44
190_F194_P1.0170.44
172_P182_A1.0160.44
246_K249_E1.0160.44
479_T483_T1.0150.44
428_P436_G1.0140.44
108_P119_P1.0120.44
492_E499_W1.0120.44
474_F488_C1.0120.44
277_V556_S1.0120.44
123_L161_V1.0120.44
284_Y296_Y1.0120.44
357_L414_G1.0110.44
55_P87_Y1.0110.44
250_R406_L1.0100.43
55_P80_G1.0100.43
83_V92_V1.0090.43
344_Y349_A1.0080.43
62_Y68_P1.0080.43
448_F452_T1.0080.43
57_C103_C1.0070.43
56_L61_A1.0050.43
536_M540_V1.0050.43
281_R293_S1.0040.43
98_L133_A1.0030.43
44_P47_G1.0020.43
82_E85_Q1.0020.43
470_R474_F1.0020.43
363_L382_H1.0010.43
386_W409_G1.0010.43
463_K468_M1.0000.42
407_L469_V1.0000.42
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4qinA10.58891000.563Contact Map
4jkvA20.73171000.602Contact Map
4c7aA20.19341000.737Contact Map
1ijyA20.20911000.748Contact Map
5bpbA401000.749Contact Map
1ijxA60.21431000.751Contact Map
2mahA10.20561000.779Contact Map
4l6rA10.672599.80.845Contact Map
2z73A20.540199.70.869Contact Map
2ks9A10.552399.70.871Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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