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OPENSEQ.org

FZD6 - looser

ID: 1494602250 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 50% gaps before running GREMLIN.
Length: 574 (514)
Sequences: 742 (506.5)
Seq/Len: 1.444
Nf(neff/√len): 22.3

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.444).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 50% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
230_C234_C2.5081.00
392_K432_Y2.4761.00
403_D414_G2.3621.00
62_Y65_T2.2910.99
121_R125_E2.2120.99
256_W260_W2.1910.99
210_C214_L1.9860.98
49_C62_Y1.9790.98
54_I107_A1.8810.97
336_C411_C1.8370.96
210_C213_Q1.8220.96
265_C297_F1.7670.95
403_D415_L1.7500.94
94_C103_C1.7200.94
125_E128_R1.7160.93
103_C110_C1.6620.92
49_C57_C1.6520.92
97_E126_R1.6170.90
131_C148_C1.6050.90
55_P84_H1.5990.90
59_D99_R1.5850.89
369_W372_E1.5750.89
343_L410_V1.5720.89
50_Q76_Q1.5630.88
124_C131_C1.5620.88
422_R493_Q1.5620.88
301_V304_V1.5460.88
57_C62_Y1.5220.86
382_H386_W1.5000.85
365_A372_E1.4940.85
386_W389_P1.4920.85
385_A389_P1.4880.85
281_R284_Y1.4570.83
110_C124_C1.4520.83
159_I163_Q1.4460.82
109_V161_V1.4340.82
544_T549_W1.4330.82
196_G199_D1.4240.81
80_G85_Q1.4160.81
118_P159_I1.4030.80
63_N73_H1.3880.79
98_L102_L1.3820.78
282_F287_R1.3770.78
94_C110_C1.3670.77
103_C124_C1.3640.77
282_F286_E1.3570.77
531_M549_W1.3570.77
282_F290_I1.3540.76
525_P530_F1.3470.76
499_W525_P1.3430.75
384_A427_A1.3370.75
80_G84_H1.3290.74
461_T464_L1.3290.74
268_T290_I1.3290.74
102_L106_Y1.3230.74
132_E136_N1.3230.74
548_I554_L1.3230.74
198_S202_G1.3210.74
284_Y295_C1.3200.74
281_R285_P1.3140.73
275_Y287_R1.3130.73
340_F344_Y1.2950.72
109_V138_F1.2870.71
127_A135_M1.2800.70
126_R130_G1.2790.70
550_S553_T1.2770.70
545_G549_W1.2760.70
252_F260_W1.2740.70
414_G421_L1.2680.69
541_G547_W1.2680.69
272_V297_F1.2670.69
492_E496_R1.2640.69
354_W361_W1.2610.69
264_C281_R1.2570.68
411_C428_P1.2560.68
353_W361_W1.2540.68
81_L84_H1.2530.68
361_W382_H1.2530.68
548_I553_T1.2450.67
177_T180_P1.2430.67
451_R464_L1.2410.67
106_Y140_F1.2390.67
535_L539_I1.2380.67
436_G481_P1.2360.66
49_C52_I1.2320.66
285_P382_H1.2280.66
158_E163_Q1.2240.65
526_D538_M1.2230.65
135_M140_F1.2220.65
285_P386_W1.2210.65
302_A306_G1.2200.65
496_R500_E1.2180.65
444_F545_G1.2180.65
274_T281_R1.2170.65
422_R427_A1.2160.65
274_T284_Y1.2160.65
50_Q54_I1.2150.65
274_T278_D1.2130.64
405_D409_G1.2130.64
124_C148_C1.2120.64
354_W436_G1.2110.64
258_G262_V1.2080.64
370_G373_A1.2030.63
553_T557_W1.2030.63
538_M550_S1.2020.63
437_T448_F1.2010.63
464_L468_M1.2010.63
229_D233_P1.2010.63
353_W386_W1.1990.63
285_P291_F1.1980.63
341_M345_F1.1950.63
209_S215_K1.1890.62
412_Y425_V1.1890.62
294_G298_M1.1890.62
278_D285_P1.1790.61
67_L76_Q1.1770.61
80_G83_V1.1760.61
65_T76_Q1.1710.60
100_F161_V1.1690.60
73_H81_L1.1680.60
89_L102_L1.1640.60
372_E382_H1.1630.60
50_Q53_S1.1630.60
73_H79_A1.1620.59
365_A368_K1.1590.59
90_V105_M1.1580.59
345_F393_T1.1530.59
424_F433_L1.1520.58
119_P132_E1.1480.58
196_G203_R1.1440.58
353_W382_H1.1440.58
357_L361_W1.1420.57
110_C131_C1.1380.57
281_R286_E1.1370.57
363_L369_W1.1340.57
287_R295_C1.1340.57
357_L389_P1.1320.56
49_C65_T1.1290.56
204_P208_F1.1290.56
448_F452_T1.1260.56
252_F256_W1.1260.56
68_P79_A1.1250.56
103_C131_C1.1250.56
73_H90_V1.1240.56
109_V123_L1.1220.55
284_Y287_R1.1210.55
85_Q90_V1.1190.55
305_A309_L1.1180.55
439_F467_L1.1110.54
268_T352_I1.1110.54
259_V313_A1.1100.54
336_C344_Y1.1090.54
470_R534_Y1.1080.54
360_T385_A1.1070.54
125_E129_Q1.1060.54
85_Q97_E1.1040.54
45_D75_N1.1030.54
84_H92_V1.1010.53
86_F90_V1.0980.53
211_P222_Y1.0970.53
127_A130_G1.0960.53
95_S120_C1.0960.53
491_Y499_W1.0950.53
68_P73_H1.0940.53
196_G201_R1.0930.52
485_V536_M1.0920.52
282_F285_P1.0910.52
131_C143_P1.0910.52
208_F213_Q1.0910.52
414_G417_S1.0890.52
289_I432_Y1.0880.52
486_L544_T1.0880.52
365_A369_W1.0870.52
471_I548_I1.0840.52
117_I126_R1.0830.51
57_C65_T1.0820.51
363_L374_I1.0790.51
67_L70_L1.0770.51
52_I76_Q1.0760.51
204_P207_P1.0750.51
536_M540_V1.0740.51
282_F288_P1.0740.51
120_C124_C1.0740.51
198_S201_R1.0730.50
55_P60_I1.0710.50
47_G67_L1.0700.50
97_E130_G1.0700.50
196_G202_G1.0690.50
488_C492_E1.0680.50
63_N68_P1.0680.50
492_E533_K1.0670.50
450_I548_I1.0660.50
44_P47_G1.0650.50
344_Y349_A1.0650.50
392_K423_G1.0640.50
104_S125_E1.0620.49
77_E83_V1.0620.49
291_F300_A1.0600.49
348_M392_K1.0590.49
293_S378_S1.0580.49
195_P198_S1.0560.49
336_C353_W1.0560.49
428_P436_G1.0550.49
69_N79_A1.0550.49
94_C124_C1.0540.49
437_T476_V1.0530.48
379_Q383_L1.0460.48
54_I63_N1.0440.47
295_C302_A1.0440.47
275_Y284_Y1.0440.47
234_C315_C1.0430.47
349_A353_W1.0430.47
344_Y405_D1.0430.47
536_M552_K1.0430.47
396_I413_V1.0420.47
55_P80_G1.0380.47
111_T146_L1.0380.47
336_C386_W1.0350.47
298_M301_V1.0340.46
123_L138_F1.0330.46
528_T546_F1.0320.46
190_F194_P1.0310.46
129_Q132_E1.0310.46
123_L161_V1.0300.46
189_P193_L1.0290.46
274_T285_P1.0290.46
56_L90_V1.0280.46
471_I475_S1.0270.46
62_Y69_N1.0270.46
330_G387_A1.0260.46
162_G166_S1.0240.45
101_F108_P1.0240.45
464_L481_P1.0230.45
256_W267_S1.0230.45
312_R315_C1.0220.45
58_T86_F1.0220.45
277_V556_S1.0210.45
260_W264_C1.0210.45
55_P83_V1.0190.45
387_A390_A1.0190.45
354_W481_P1.0170.45
177_T182_A1.0170.45
56_L61_A1.0160.45
148_C160_C1.0160.45
373_A552_K1.0160.45
86_F140_F1.0150.45
315_C318_R1.0150.45
270_F273_L1.0140.44
82_E85_Q1.0130.44
453_I469_V1.0130.44
284_Y291_F1.0120.44
332_K413_V1.0120.44
172_P182_A1.0120.44
117_I159_I1.0120.44
471_I474_F1.0120.44
67_L72_G1.0120.44
98_L128_R1.0110.44
94_C131_C1.0090.44
59_D78_D1.0090.44
169_S172_P1.0080.44
98_L133_A1.0080.44
143_P148_C1.0050.44
295_C349_A1.0050.44
55_P87_Y1.0050.44
363_L382_H1.0050.44
175_G180_P1.0040.43
105_M146_L1.0040.43
402_V486_L1.0020.43
143_P146_L1.0010.43
108_P119_P1.0000.43
474_F488_C1.0000.43
481_P552_K1.0000.43
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4qinA10.58891000.576Contact Map
4jkvA20.73171000.615Contact Map
4c7aA20.19341000.725Contact Map
1ijyA20.20911000.736Contact Map
5bpbA401000.736Contact Map
1ijxA60.21431000.739Contact Map
2mahA10.20561000.769Contact Map
4l6rA10.67699.90.822Contact Map
4dklA10.482699.90.829Contact Map
3vw7A10.479199.90.829Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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