| NOB_hypo1 |
| ID: | 1491500445 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 866 (832) | ||
| Sequences: | 4124 (3049.7) | ||
| Seq/Len: | 4.957 | ||
| Nf(neff/√len): | 105.7 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 4.957).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 785_L | 788_E | 4.919 | 1.00 |
| 99_S | 102_K | 4.798 | 1.00 |
| 59_Q | 62_K | 4.776 | 1.00 |
| 585_D | 588_N | 4.600 | 1.00 |
| 266_K | 269_E | 4.447 | 1.00 |
| 305_Q | 308_L | 4.445 | 1.00 |
| 745_E | 748_N | 4.391 | 1.00 |
| 505_D | 508_K | 4.334 | 1.00 |
| 139_E | 142_Q | 4.315 | 1.00 |
| 625_D | 628_Q | 4.243 | 1.00 |
| 545_K | 548_K | 4.212 | 1.00 |
| 220_R | 223_K | 4.159 | 1.00 |
| 465_E | 468_E | 4.118 | 1.00 |
| 665_E | 668_K | 4.029 | 1.00 |
| 19_N | 22_D | 3.980 | 1.00 |
| 705_Y | 708_K | 3.750 | 1.00 |
| 825_R | 828_S | 3.598 | 1.00 |
| 345_D | 348_G | 3.508 | 1.00 |
| 385_E | 388_Q | 3.280 | 1.00 |
| 131_G | 147_Y | 2.929 | 1.00 |
| 497_G | 513_Y | 2.638 | 1.00 |
| 817_A | 833_I | 2.576 | 1.00 |
| 790_E | 816_V | 2.554 | 1.00 |
| 737_G | 753_Y | 2.445 | 1.00 |
| 617_Y | 633_A | 2.394 | 1.00 |
| 297_G | 313_Y | 2.384 | 1.00 |
| 457_G | 473_A | 2.348 | 1.00 |
| 657_S | 673_Y | 2.336 | 1.00 |
| 777_G | 793_F | 2.306 | 1.00 |
| 104_E | 130_L | 2.301 | 1.00 |
| 94_Y | 102_K | 2.251 | 1.00 |
| 296_L | 312_Y | 2.219 | 1.00 |
| 780_K | 788_E | 2.132 | 1.00 |
| 21_E | 57_S | 2.058 | 1.00 |
| 377_G | 393_L | 2.036 | 1.00 |
| 460_L | 468_E | 2.026 | 1.00 |
| 64_E | 90_L | 2.024 | 1.00 |
| 337_G | 353_Y | 1.995 | 1.00 |
| 50_L | 54_F | 1.973 | 1.00 |
| 101_E | 137_K | 1.946 | 1.00 |
| 130_L | 134_C | 1.876 | 1.00 |
| 500_F | 508_K | 1.847 | 1.00 |
| 51_G | 67_Y | 1.844 | 1.00 |
| 310_W | 336_L | 1.840 | 1.00 |
| 537_L | 553_V | 1.839 | 1.00 |
| 577_G | 593_Y | 1.831 | 1.00 |
| 90_L | 94_Y | 1.829 | 1.00 |
| 297_G | 316_S | 1.814 | 1.00 |
| 580_C | 588_N | 1.813 | 1.00 |
| 697_G | 713_F | 1.804 | 1.00 |
| 456_L | 460_L | 1.798 | 1.00 |
| 747_K | 783_D | 1.787 | 1.00 |
| 24_E | 50_L | 1.775 | 1.00 |
| 337_G | 356_S | 1.754 | 1.00 |
| 550_E | 576_L | 1.751 | 1.00 |
| 134_C | 142_Q | 1.738 | 1.00 |
| 337_G | 352_Q | 1.724 | 1.00 |
| 91_G | 107_Y | 1.713 | 1.00 |
| 353_Y | 375_S | 1.712 | 1.00 |
| 670_E | 696_L | 1.701 | 1.00 |
| 620_Y | 628_Q | 1.695 | 0.99 |
| 271_E | 296_L | 1.635 | 0.99 |
| 11_G | 27_F | 1.630 | 0.99 |
| 620_Y | 625_D | 1.627 | 0.99 |
| 64_E | 97_Q | 1.621 | 0.99 |
| 142_Q | 145_D | 1.610 | 0.99 |
| 497_G | 512_N | 1.605 | 0.99 |
| 62_K | 65_D | 1.602 | 0.99 |
| 780_K | 792_Y | 1.601 | 0.99 |
| 212_E | 228_I | 1.584 | 0.99 |
| 350_K | 376_L | 1.583 | 0.99 |
| 577_G | 596_A | 1.569 | 0.99 |
| 660_Y | 668_K | 1.561 | 0.99 |
| 296_L | 300_Y | 1.555 | 0.99 |
| 736_L | 740_A | 1.550 | 0.99 |
| 271_E | 275_K | 1.550 | 0.99 |
| 776_L | 780_K | 1.537 | 0.99 |
| 468_E | 471_D | 1.527 | 0.99 |
| 773_L | 792_Y | 1.527 | 0.99 |
| 510_E | 536_Y | 1.513 | 0.99 |
| 300_Y | 308_L | 1.509 | 0.99 |
| 510_E | 539_V | 1.508 | 0.99 |
| 271_E | 303_K | 1.507 | 0.99 |
| 536_Y | 540_L | 1.503 | 0.98 |
| 576_L | 580_C | 1.485 | 0.98 |
| 275_K | 296_L | 1.484 | 0.98 |
| 737_G | 756_A | 1.477 | 0.98 |
| 223_K | 226_E | 1.476 | 0.98 |
| 740_A | 748_N | 1.474 | 0.98 |
| 748_N | 751_D | 1.469 | 0.98 |
| 54_F | 62_K | 1.468 | 0.98 |
| 668_K | 671_D | 1.456 | 0.98 |
| 587_F | 623_K | 1.456 | 0.98 |
| 310_W | 339_V | 1.452 | 0.98 |
| 590_E | 623_K | 1.445 | 0.98 |
| 271_E | 299_I | 1.444 | 0.98 |
| 708_K | 711_E | 1.434 | 0.98 |
| 554_K | 576_L | 1.429 | 0.98 |
| 211_L | 215_Y | 1.426 | 0.98 |
| 390_E | 394_M | 1.425 | 0.98 |
| 794_N | 798_S | 1.420 | 0.98 |
| 630_E | 656_L | 1.416 | 0.98 |
| 24_E | 53_I | 1.406 | 0.97 |
| 102_K | 105_E | 1.406 | 0.97 |
| 493_L | 512_N | 1.403 | 0.97 |
| 104_E | 137_K | 1.394 | 0.97 |
| 380_F | 388_Q | 1.388 | 0.97 |
| 29_E | 32_K | 1.386 | 0.97 |
| 497_G | 516_A | 1.385 | 0.97 |
| 590_E | 616_I | 1.384 | 0.97 |
| 251_L | 255_C | 1.377 | 0.97 |
| 788_E | 791_D | 1.376 | 0.97 |
| 750_E | 776_L | 1.375 | 0.97 |
| 61_D | 97_Q | 1.374 | 0.97 |
| 252_A | 274_F | 1.349 | 0.96 |
| 94_Y | 99_S | 1.349 | 0.96 |
| 308_L | 348_G | 1.348 | 0.96 |
| 355_K | 358_E | 1.346 | 0.96 |
| 776_L | 792_Y | 1.345 | 0.96 |
| 500_F | 512_N | 1.344 | 0.96 |
| 376_L | 380_F | 1.344 | 0.96 |
| 510_E | 514_A | 1.337 | 0.96 |
| 212_E | 224_V | 1.335 | 0.96 |
| 380_F | 385_E | 1.334 | 0.96 |
| 144_I | 148_Q | 1.333 | 0.96 |
| 358_E | 478_A | 1.332 | 0.96 |
| 64_E | 93_V | 1.329 | 0.96 |
| 816_V | 820_E | 1.326 | 0.96 |
| 634_L | 656_L | 1.326 | 0.96 |
| 394_M | 398_D | 1.321 | 0.96 |
| 293_L | 312_Y | 1.315 | 0.96 |
| 510_E | 543_V | 1.315 | 0.96 |
| 91_G | 110_A | 1.306 | 0.96 |
| 740_A | 745_E | 1.306 | 0.96 |
| 710_G | 736_L | 1.306 | 0.96 |
| 740_A | 752_T | 1.302 | 0.95 |
| 616_I | 632_F | 1.301 | 0.95 |
| 229_Q | 233_A | 1.296 | 0.95 |
| 354_F | 358_E | 1.291 | 0.95 |
| 64_E | 68_S | 1.284 | 0.95 |
| 269_E | 272_A | 1.282 | 0.95 |
| 750_E | 783_D | 1.282 | 0.95 |
| 24_E | 54_F | 1.281 | 0.95 |
| 380_F | 392_Y | 1.279 | 0.95 |
| 580_C | 585_D | 1.279 | 0.95 |
| 638_S | 718_A | 1.275 | 0.95 |
| 330_A | 356_S | 1.274 | 0.95 |
| 613_N | 632_F | 1.272 | 0.95 |
| 753_Y | 775_Y | 1.272 | 0.95 |
| 67_Y | 89_N | 1.270 | 0.95 |
| 470_C | 496_L | 1.268 | 0.94 |
| 660_Y | 665_E | 1.267 | 0.94 |
| 473_A | 553_V | 1.265 | 0.94 |
| 594_L | 616_I | 1.261 | 0.94 |
| 634_L | 668_K | 1.261 | 0.94 |
| 755_K | 758_A | 1.253 | 0.94 |
| 730_A | 756_A | 1.252 | 0.94 |
| 678_E | 758_A | 1.250 | 0.94 |
| 353_Y | 473_A | 1.250 | 0.94 |
| 674_K | 678_E | 1.249 | 0.94 |
| 474_K | 496_L | 1.248 | 0.94 |
| 94_Y | 106_S | 1.248 | 0.94 |
| 577_G | 589_A | 1.248 | 0.94 |
| 54_F | 59_Q | 1.248 | 0.94 |
| 700_N | 708_K | 1.246 | 0.94 |
| 352_Q | 512_N | 1.244 | 0.94 |
| 134_C | 146_F | 1.244 | 0.94 |
| 134_C | 540_L | 1.242 | 0.94 |
| 297_G | 309_A | 1.239 | 0.94 |
| 350_K | 379_I | 1.239 | 0.94 |
| 540_L | 548_K | 1.234 | 0.93 |
| 501_M | 513_Y | 1.227 | 0.93 |
| 790_E | 793_F | 1.223 | 0.93 |
| 134_C | 620_Y | 1.220 | 0.93 |
| 713_F | 735_N | 1.218 | 0.93 |
| 388_Q | 391_D | 1.217 | 0.93 |
| 516_A | 676_A | 1.207 | 0.92 |
| 657_S | 672_Y | 1.204 | 0.92 |
| 300_Y | 312_Y | 1.203 | 0.92 |
| 25_S | 29_E | 1.202 | 0.92 |
| 271_E | 300_Y | 1.202 | 0.92 |
| 496_L | 500_F | 1.202 | 0.92 |
| 696_L | 700_N | 1.201 | 0.92 |
| 816_V | 832_F | 1.200 | 0.92 |
| 276_K | 279_E | 1.198 | 0.92 |
| 590_E | 619_L | 1.198 | 0.92 |
| 813_Y | 832_F | 1.197 | 0.92 |
| 817_A | 832_F | 1.194 | 0.92 |
| 275_K | 308_L | 1.191 | 0.92 |
| 820_E | 828_S | 1.189 | 0.92 |
| 475_E | 635_K | 1.189 | 0.92 |
| 617_Y | 632_F | 1.187 | 0.92 |
| 754_L | 776_L | 1.184 | 0.91 |
| 577_G | 592_N | 1.184 | 0.91 |
| 589_A | 593_Y | 1.182 | 0.91 |
| 470_C | 499_I | 1.181 | 0.91 |
| 340_K | 345_D | 1.179 | 0.91 |
| 55_F | 60_F | 1.178 | 0.91 |
| 60_F | 93_V | 1.176 | 0.91 |
| 225_A | 251_L | 1.176 | 0.91 |
| 314_E | 336_L | 1.172 | 0.91 |
| 701_I | 739_L | 1.172 | 0.91 |
| 514_A | 548_K | 1.171 | 0.91 |
| 134_C | 139_E | 1.168 | 0.91 |
| 391_D | 471_D | 1.168 | 0.91 |
| 478_A | 518_E | 1.167 | 0.91 |
| 313_Y | 335_N | 1.166 | 0.91 |
| 373_C | 392_Y | 1.166 | 0.91 |
| 377_G | 396_A | 1.164 | 0.90 |
| 104_E | 108_Q | 1.162 | 0.90 |
| 215_Y | 223_K | 1.161 | 0.90 |
| 255_C | 266_K | 1.161 | 0.90 |
| 230_K | 233_A | 1.159 | 0.90 |
| 131_G | 150_A | 1.156 | 0.90 |
| 108_Q | 130_L | 1.154 | 0.90 |
| 274_F | 295_S | 1.153 | 0.90 |
| 590_E | 594_L | 1.152 | 0.90 |
| 496_L | 512_N | 1.149 | 0.90 |
| 710_G | 739_L | 1.147 | 0.90 |
| 334_A | 338_N | 1.144 | 0.89 |
| 334_A | 375_S | 1.144 | 0.89 |
| 548_K | 551_K | 1.143 | 0.89 |
| 12_Y | 27_F | 1.140 | 0.89 |
| 131_G | 143_A | 1.140 | 0.89 |
| 104_E | 133_T | 1.137 | 0.89 |
| 350_K | 354_F | 1.135 | 0.89 |
| 356_S | 636_S | 1.133 | 0.89 |
| 268_D | 303_K | 1.133 | 0.89 |
| 617_Y | 629_A | 1.132 | 0.89 |
| 780_K | 785_L | 1.128 | 0.89 |
| 697_G | 716_A | 1.127 | 0.89 |
| 23_A | 27_F | 1.126 | 0.88 |
| 470_C | 474_K | 1.124 | 0.88 |
| 628_Q | 631_K | 1.123 | 0.88 |
| 741_F | 779_L | 1.123 | 0.88 |
| 247_C | 532_I | 1.123 | 0.88 |
| 10_L | 14_S | 1.121 | 0.88 |
| 47_L | 66_F | 1.121 | 0.88 |
| 508_K | 511_E | 1.119 | 0.88 |
| 787_E | 823_V | 1.119 | 0.88 |
| 747_K | 787_E | 1.118 | 0.88 |
| 549_A | 553_V | 1.117 | 0.88 |
| 354_F | 380_F | 1.116 | 0.88 |
| 27_F | 49_N | 1.116 | 0.88 |
| 540_L | 545_K | 1.116 | 0.88 |
| 130_L | 146_F | 1.115 | 0.88 |
| 630_E | 660_Y | 1.114 | 0.88 |
| 212_E | 231_T | 1.111 | 0.88 |
| 678_E | 718_A | 1.109 | 0.87 |
| 533_S | 552_L | 1.108 | 0.87 |
| 558_K | 598_E | 1.105 | 0.87 |
| 517_I | 677_L | 1.105 | 0.87 |
| 493_L | 573_H | 1.104 | 0.87 |
| 559_V | 639_I | 1.104 | 0.87 |
| 815_T | 818_R | 1.104 | 0.87 |
| 470_C | 500_F | 1.103 | 0.87 |
| 467_D | 507_A | 1.103 | 0.87 |
| 576_L | 592_N | 1.102 | 0.87 |
| 22_D | 25_S | 1.102 | 0.87 |
| 11_G | 23_A | 1.101 | 0.87 |
| 135_L | 140_L | 1.099 | 0.87 |
| 638_S | 678_E | 1.098 | 0.87 |
| 14_S | 19_N | 1.098 | 0.87 |
| 547_D | 627_S | 1.098 | 0.87 |
| 290_S | 316_S | 1.092 | 0.86 |
| 340_K | 352_Q | 1.092 | 0.86 |
| 790_E | 819_M | 1.088 | 0.86 |
| 108_Q | 134_C | 1.087 | 0.86 |
| 585_D | 705_Y | 1.087 | 0.86 |
| 817_A | 836_A | 1.086 | 0.86 |
| 654_R | 695_F | 1.083 | 0.86 |
| 376_L | 576_L | 1.081 | 0.86 |
| 518_E | 718_A | 1.080 | 0.85 |
| 617_Y | 636_S | 1.080 | 0.85 |
| 337_G | 617_Y | 1.080 | 0.85 |
| 493_L | 515_R | 1.075 | 0.85 |
| 515_R | 519_L | 1.074 | 0.85 |
| 336_L | 340_K | 1.074 | 0.85 |
| 433_I | 437_L | 1.071 | 0.85 |
| 310_W | 343_Q | 1.071 | 0.85 |
| 429_R | 432_Q | 1.069 | 0.85 |
| 657_S | 669_Q | 1.068 | 0.85 |
| 457_G | 472_L | 1.068 | 0.85 |
| 72_T | 112_Q | 1.067 | 0.85 |
| 556_S | 716_A | 1.067 | 0.85 |
| 630_E | 634_L | 1.067 | 0.85 |
| 661_V | 673_Y | 1.066 | 0.84 |
| 107_Y | 553_V | 1.065 | 0.84 |
| 107_Y | 129_Y | 1.064 | 0.84 |
| 352_Q | 472_L | 1.062 | 0.84 |
| 87_L | 106_S | 1.060 | 0.84 |
| 539_V | 543_V | 1.051 | 0.83 |
| 494_G | 535_S | 1.049 | 0.83 |
| 810_I | 836_A | 1.048 | 0.83 |
| 588_N | 708_K | 1.048 | 0.83 |
| 245_G | 277_A | 1.048 | 0.83 |
| 790_E | 823_V | 1.047 | 0.83 |
| 588_N | 591_K | 1.046 | 0.83 |
| 394_M | 554_K | 1.045 | 0.83 |
| 834_T | 838_K | 1.045 | 0.83 |
| 398_D | 518_E | 1.045 | 0.83 |
| 14_S | 22_D | 1.045 | 0.83 |
| 795_Q | 799_C | 1.043 | 0.83 |
| 252_A | 277_A | 1.041 | 0.83 |
| 453_L | 472_L | 1.041 | 0.83 |
| 310_W | 314_E | 1.041 | 0.83 |
| 550_E | 554_K | 1.040 | 0.82 |
| 385_E | 545_K | 1.039 | 0.82 |
| 347_D | 747_K | 1.039 | 0.82 |
| 255_C | 269_E | 1.037 | 0.82 |
| 92_S | 107_Y | 1.037 | 0.82 |
| 28_L | 50_L | 1.036 | 0.82 |
| 310_W | 340_K | 1.035 | 0.82 |
| 835_K | 838_K | 1.035 | 0.82 |
| 750_E | 779_L | 1.035 | 0.82 |
| 279_E | 598_E | 1.035 | 0.82 |
| 620_Y | 632_F | 1.035 | 0.82 |
| 795_Q | 798_S | 1.035 | 0.82 |
| 200_Q | 203_K | 1.031 | 0.82 |
| 554_K | 674_K | 1.030 | 0.82 |
| 68_S | 90_L | 1.030 | 0.82 |
| 578_N | 615_N | 1.030 | 0.82 |
| 90_L | 106_S | 1.029 | 0.82 |
| 391_D | 791_D | 1.029 | 0.82 |
| 269_E | 308_L | 1.028 | 0.81 |
| 715_Q | 719_L | 1.027 | 0.81 |
| 536_Y | 552_L | 1.027 | 0.81 |
| 670_E | 674_K | 1.024 | 0.81 |
| 534_G | 575_N | 1.023 | 0.81 |
| 212_E | 537_L | 1.023 | 0.81 |
| 707_E | 743_N | 1.022 | 0.81 |
| 17_K | 137_K | 1.022 | 0.81 |
| 293_L | 573_H | 1.021 | 0.81 |
| 591_K | 631_K | 1.021 | 0.81 |
| 460_L | 465_E | 1.021 | 0.81 |
| 777_G | 792_Y | 1.019 | 0.81 |
| 152_E | 718_A | 1.019 | 0.81 |
| 693_L | 715_Q | 1.018 | 0.81 |
| 820_E | 825_R | 1.017 | 0.80 |
| 308_L | 311_E | 1.017 | 0.80 |
| 786_F | 819_M | 1.016 | 0.80 |
| 267_V | 346_W | 1.016 | 0.80 |
| 70_S | 86_S | 1.015 | 0.80 |
| 537_L | 552_L | 1.015 | 0.80 |
| 145_D | 391_D | 1.015 | 0.80 |
| 794_N | 828_S | 1.013 | 0.80 |
| 216_F | 221_M | 1.012 | 0.80 |
| 696_L | 712_Y | 1.011 | 0.80 |
| 593_Y | 615_N | 1.010 | 0.80 |
| 476_T | 636_S | 1.009 | 0.80 |
| 65_D | 69_R | 1.008 | 0.80 |
| 473_A | 633_A | 1.008 | 0.80 |
| 315_K | 635_K | 1.008 | 0.80 |
| 313_Y | 593_Y | 1.008 | 0.80 |
| 251_L | 536_Y | 1.007 | 0.80 |
| 656_L | 660_Y | 1.007 | 0.80 |
| 354_F | 376_L | 1.006 | 0.79 |
| 340_K | 348_G | 1.005 | 0.79 |
| 630_E | 710_G | 1.003 | 0.79 |
| 149_K | 475_E | 1.001 | 0.79 |
| 587_F | 591_K | 1.001 | 0.79 |
| 653_C | 733_Y | 1.000 | 0.79 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4bujB | 2 | 0.8707 | 100 | 0.396 | Contact Map |
| 4kvmA | 4 | 0.7552 | 100 | 0.486 | Contact Map |
| 4hnyA | 2 | 0.7194 | 100 | 0.493 | Contact Map |
| 4m59A | 2 | 0.7737 | 100 | 0.546 | Contact Map |
| 4uzyA | 1 | 0.6894 | 100 | 0.548 | Contact Map |
| 4rg9A | 2 | 0.537 | 100 | 0.553 | Contact Map |
| 4ui9J | 1 | 0 | 100 | 0.555 | Contact Map |
| 2xpiA | 2 | 0.5774 | 100 | 0.561 | Contact Map |
| 4ui9X | 2 | 0 | 100 | 0.565 | Contact Map |
| 1w3bA | 2 | 0.448 | 100 | 0.571 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)