| ASPA |
| ID: | 1491222695 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 313 (285) | ||
| Sequences: | 2509 (1472.7) | ||
| Seq/Len: | 8.804 | ||
| Nf(neff/√len): | 87.2 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 8.804).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 14_V | 206_I | 4.435 | 1.00 |
| 260_P | 271_P | 3.892 | 1.00 |
| 260_P | 269_T | 3.729 | 1.00 |
| 256_H | 275_D | 3.376 | 1.00 |
| 249_D | 259_D | 3.243 | 1.00 |
| 256_H | 259_D | 2.947 | 1.00 |
| 243_I | 262_F | 2.763 | 1.00 |
| 138_Q | 208_H | 2.737 | 1.00 |
| 94_Q | 98_H | 2.711 | 1.00 |
| 35_L | 50_P | 2.578 | 1.00 |
| 250_Q | 253_K | 2.447 | 1.00 |
| 137_I | 141_H | 2.359 | 1.00 |
| 207_H | 214_E | 2.336 | 1.00 |
| 13_K | 108_S | 2.285 | 1.00 |
| 9_E | 43_R | 2.239 | 1.00 |
| 14_V | 211_E | 2.209 | 1.00 |
| 31_V | 50_P | 2.197 | 1.00 |
| 139_M | 175_V | 2.122 | 1.00 |
| 16_I | 31_V | 2.083 | 1.00 |
| 97_N | 166_T | 1.991 | 1.00 |
| 15_A | 109_Y | 1.923 | 1.00 |
| 13_K | 45_G | 1.922 | 1.00 |
| 15_A | 112_I | 1.880 | 1.00 |
| 263_L | 269_T | 1.791 | 1.00 |
| 53_T | 96_I | 1.774 | 1.00 |
| 51_F | 96_I | 1.766 | 1.00 |
| 22_G | 57_A | 1.747 | 1.00 |
| 111_I | 209_F | 1.741 | 1.00 |
| 26_T | 117_N | 1.711 | 1.00 |
| 42_Q | 211_E | 1.704 | 1.00 |
| 113_F | 205_F | 1.678 | 1.00 |
| 248_Q | 263_L | 1.609 | 1.00 |
| 221_E | 300_K | 1.607 | 1.00 |
| 33_H | 196_R | 1.597 | 1.00 |
| 262_F | 294_A | 1.578 | 1.00 |
| 142_Y | 208_H | 1.509 | 1.00 |
| 116_H | 178_E | 1.469 | 1.00 |
| 46_L | 211_E | 1.460 | 1.00 |
| 19_G | 28_V | 1.453 | 0.99 |
| 34_W | 50_P | 1.450 | 0.99 |
| 272_L | 275_D | 1.405 | 0.99 |
| 278_V | 297_K | 1.405 | 0.99 |
| 280_P | 295_F | 1.402 | 0.99 |
| 114_D | 178_E | 1.401 | 0.99 |
| 260_P | 263_L | 1.397 | 0.99 |
| 224_K | 299_T | 1.389 | 0.99 |
| 261_M | 272_L | 1.385 | 0.99 |
| 13_K | 49_K | 1.383 | 0.99 |
| 17_F | 20_T | 1.371 | 0.99 |
| 223_Y | 298_T | 1.365 | 0.99 |
| 33_H | 194_Q | 1.345 | 0.99 |
| 63_R | 71_R | 1.342 | 0.99 |
| 204_D | 207_H | 1.341 | 0.99 |
| 77_N | 94_Q | 1.334 | 0.99 |
| 264_T | 270_I | 1.323 | 0.99 |
| 249_D | 280_P | 1.310 | 0.99 |
| 21_H | 24_E | 1.307 | 0.99 |
| 117_N | 182_Q | 1.306 | 0.99 |
| 261_M | 297_K | 1.305 | 0.99 |
| 21_H | 116_H | 1.294 | 0.99 |
| 37_N | 203_L | 1.291 | 0.99 |
| 257_P | 275_D | 1.283 | 0.98 |
| 63_R | 70_N | 1.269 | 0.98 |
| 14_V | 209_F | 1.257 | 0.98 |
| 56_R | 88_Y | 1.254 | 0.98 |
| 70_N | 114_D | 1.249 | 0.98 |
| 115_L | 202_A | 1.243 | 0.98 |
| 28_V | 58_V | 1.242 | 0.98 |
| 11_I | 44_T | 1.241 | 0.98 |
| 68_D | 128_L | 1.236 | 0.98 |
| 33_H | 199_I | 1.234 | 0.98 |
| 16_I | 115_L | 1.227 | 0.98 |
| 192_L | 199_I | 1.225 | 0.98 |
| 17_F | 53_T | 1.220 | 0.98 |
| 14_V | 111_I | 1.214 | 0.98 |
| 32_K | 36_E | 1.213 | 0.98 |
| 255_L | 259_D | 1.200 | 0.97 |
| 20_T | 53_T | 1.191 | 0.97 |
| 111_I | 173_Y | 1.190 | 0.97 |
| 13_K | 110_D | 1.185 | 0.97 |
| 64_Y | 68_D | 1.183 | 0.97 |
| 23_N | 63_R | 1.183 | 0.97 |
| 259_D | 280_P | 1.176 | 0.97 |
| 245_P | 248_Q | 1.168 | 0.97 |
| 96_I | 100_F | 1.159 | 0.97 |
| 17_F | 112_I | 1.158 | 0.96 |
| 43_R | 213_K | 1.153 | 0.96 |
| 134_N | 138_Q | 1.145 | 0.96 |
| 243_I | 294_A | 1.145 | 0.96 |
| 67_C | 72_I | 1.136 | 0.96 |
| 68_D | 117_N | 1.136 | 0.96 |
| 46_L | 206_I | 1.134 | 0.96 |
| 123_G | 223_Y | 1.129 | 0.96 |
| 293_E | 296_A | 1.126 | 0.96 |
| 111_I | 139_M | 1.125 | 0.96 |
| 257_P | 276_C | 1.121 | 0.96 |
| 87_P | 90_V | 1.113 | 0.95 |
| 119_T | 284_N | 1.111 | 0.95 |
| 101_G | 170_I | 1.109 | 0.95 |
| 113_F | 202_A | 1.104 | 0.95 |
| 74_D | 86_L | 1.100 | 0.95 |
| 248_Q | 260_P | 1.095 | 0.95 |
| 57_A | 89_E | 1.092 | 0.95 |
| 204_D | 214_E | 1.082 | 0.94 |
| 34_W | 203_L | 1.081 | 0.94 |
| 29_F | 32_K | 1.079 | 0.94 |
| 253_K | 259_D | 1.075 | 0.94 |
| 276_C | 299_T | 1.069 | 0.94 |
| 24_E | 70_N | 1.061 | 0.94 |
| 98_H | 102_P | 1.060 | 0.93 |
| 251_D | 283_V | 1.058 | 0.93 |
| 21_H | 63_R | 1.056 | 0.93 |
| 18_G | 31_V | 1.053 | 0.93 |
| 207_H | 213_K | 1.047 | 0.93 |
| 16_I | 50_P | 1.044 | 0.93 |
| 51_F | 109_Y | 1.043 | 0.93 |
| 25_L | 58_V | 1.038 | 0.93 |
| 231_Y | 296_A | 1.035 | 0.92 |
| 97_N | 170_I | 1.033 | 0.92 |
| 95_E | 99_L | 1.027 | 0.92 |
| 13_K | 47_E | 1.024 | 0.92 |
| 63_R | 68_D | 1.024 | 0.92 |
| 48_V | 105_S | 1.023 | 0.92 |
| 284_N | 294_A | 1.022 | 0.92 |
| 71_R | 114_D | 1.022 | 0.92 |
| 203_L | 207_H | 1.018 | 0.92 |
| 200_K | 204_D | 1.012 | 0.91 |
| 249_D | 253_K | 1.011 | 0.91 |
| 122_M | 282_F | 1.009 | 0.91 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- See coupling matrix (for protein design or mutagenesis)
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3nh4A | 1 | 0.9521 | 100 | 0.362 | Contact Map |
| 2gu2A | 2 | 0.9489 | 100 | 0.367 | Contact Map |
| 1yw4A | 2 | 0.8818 | 100 | 0.455 | Contact Map |
| 3cdxA | 6 | 0.869 | 100 | 0.456 | Contact Map |
| 3na6A | 1 | 0.8626 | 100 | 0.463 | Contact Map |
| 2qj8A | 2 | 0.8594 | 100 | 0.475 | Contact Map |
| 1yw6A | 2 | 0.8754 | 100 | 0.492 | Contact Map |
| 2bcoA | 2 | 0.8914 | 100 | 0.505 | Contact Map |
| 3fmcA | 4 | 0.8818 | 100 | 0.515 | Contact Map |
| 4wckA | 1 | 0.8754 | 100 | 0.626 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)