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OPENSEQ.org

EAT-4

ID: 1486603014 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 576 (448)
Sequences: 490 (349.3)
Seq/Len: 1.094
Nf(neff/√len): 16.5

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.094).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
233_L241_A2.9681.00
262_F269_P2.7191.00
316_A484_G2.1440.98
375_S378_A2.0880.97
465_H469_G2.0750.97
199_W215_A2.0340.97
67_R70_W2.0250.97
96_H234_V2.0210.96
159_A250_G1.9260.95
435_R500_A1.8960.94
310_I432_I1.8570.94
305_K487_F1.8330.93
320_T481_H1.8130.93
380_R431_D1.7920.92
367_Y371_N1.7060.89
377_T493_S1.6750.88
369_R376_T1.6690.88
400_Y475_L1.6570.87
143_A439_I1.6570.87
79_G253_W1.6500.87
299_K303_T1.6070.85
376_T494_G1.5590.83
144_K331_Y1.5360.81
312_V487_F1.5360.81
276_K280_E1.5190.80
314_N448_G1.5140.80
165_L184_Q1.5120.80
97_M169_F1.5030.79
405_T409_M1.4970.79
433_A500_A1.4940.79
385_C489_A1.4930.79
133_V137_P1.4900.78
304_S499_W1.4810.78
136_I229_L1.4690.77
362_G366_D1.4670.77
72_L261_T1.4650.77
353_V416_G1.4560.76
266_A280_E1.4530.76
202_W424_G1.4510.76
396_L478_S1.4500.76
98_Y120_L1.4410.75
228_P419_G1.4340.75
99_K114_N1.4310.74
374_L379_V1.4270.74
68_K268_H1.4090.73
152_G257_W1.3930.72
309_A491_Y1.3880.71
162_N184_Q1.3850.71
97_M113_F1.3720.70
361_G420_F1.3700.70
99_K111_H1.3700.70
125_S453_L1.3650.69
374_L378_A1.3640.69
93_A243_F1.3470.68
67_R234_V1.3440.68
146_P323_L1.3410.67
128_F196_H1.3400.67
140_F144_K1.3400.67
67_R96_H1.3350.67
331_Y461_A1.3270.66
84_F314_N1.3210.66
233_L242_P1.3150.65
428_N431_D1.3140.65
73_A211_L1.3060.64
158_G188_Q1.2960.63
138_A189_G1.2710.61
445_N452_G1.2710.61
90_F459_T1.2670.61
409_M413_A1.2660.61
131_Y445_N1.2600.60
324_L415_V1.2570.60
127_Y131_Y1.2570.60
173_S176_L1.2560.60
377_T488_Y1.2520.60
127_Y146_P1.2440.59
220_Y358_V1.2430.59
279_I282_A1.2410.58
129_Y150_L1.2410.58
94_K98_Y1.2390.58
268_H436_Y1.2370.58
384_N429_H1.2300.57
134_T189_G1.2250.57
136_I492_A1.2240.57
254_A258_F1.2190.56
162_N165_L1.2160.56
194_A264_K1.2140.56
358_V422_I1.2140.56
480_I503_K1.2100.56
127_Y307_V1.2070.55
203_A211_L1.2070.55
356_C413_A1.2060.55
368_L373_I1.2060.55
357_V416_G1.2050.55
377_T494_G1.2040.55
182_I208_R1.2020.55
218_G434_P1.2010.55
227_L279_I1.1970.54
115_W434_P1.1910.54
151_F188_Q1.1900.54
441_M445_N1.1890.54
209_S365_A1.1890.54
187_V220_Y1.1880.54
201_Y264_K1.1860.53
102_T111_H1.1850.53
128_F429_H1.1840.53
418_S442_G1.1820.53
199_W211_L1.1820.53
428_N432_I1.1810.53
157_V299_K1.1800.53
249_C288_N1.1750.52
77_N146_P1.1750.52
347_A454_T1.1730.52
160_F252_I1.1670.52
139_G142_A1.1670.52
394_F410_A1.1620.51
141_L144_K1.1620.51
68_K270_T1.1450.49
429_H444_S1.1450.49
377_T381_K1.1450.49
130_G142_A1.1440.49
323_L455_C1.1420.49
155_I253_W1.1380.49
137_P142_A1.1370.49
112_E170_K1.1350.48
196_H279_I1.1320.48
168_G180_I1.1320.48
211_L215_A1.1320.48
387_G424_G1.1320.48
196_H429_H1.1320.48
211_L427_V1.1240.47
222_G380_R1.1240.47
99_K102_T1.1220.47
87_R127_Y1.1220.47
225_L352_L1.1180.47
233_L245_L1.1160.47
189_G253_W1.1160.47
442_G484_G1.1150.47
90_F184_Q1.1140.47
90_F177_V1.1140.47
181_Q329_L1.1110.46
226_G246_Y1.1100.46
116_T120_L1.1100.46
157_V248_V1.1080.46
141_L312_V1.1080.46
104_P396_L1.1080.46
347_A350_P1.1060.46
137_P349_I1.1050.46
67_R71_L1.1050.46
328_Q398_V1.1040.46
134_T186_L1.1040.46
429_H440_L1.1010.45
309_A487_F1.1010.45
318_S451_A1.1000.45
324_L440_L1.0990.45
92_A325_L1.0980.45
299_K331_Y1.0960.45
82_I197_G1.0950.45
384_N426_N1.0940.45
411_L442_G1.0920.44
80_F151_F1.0920.44
102_T114_N1.0920.44
152_G365_A1.0890.44
380_R428_N1.0880.44
116_T490_V1.0850.44
475_L479_L1.0840.44
128_F131_Y1.0810.43
302_V364_L1.0790.43
230_S412_I1.0780.43
435_R439_I1.0780.43
139_G438_A1.0770.43
266_A462_F1.0770.43
115_W218_G1.0740.43
378_A382_I1.0730.43
95_T169_F1.0710.42
217_T355_G1.0690.42
376_T428_N1.0670.42
374_L382_I1.0670.42
220_Y234_V1.0660.42
162_N234_V1.0650.42
184_Q187_V1.0650.42
94_K120_L1.0650.42
256_L311_I1.0640.42
114_N296_I1.0630.42
121_S222_G1.0610.42
202_W275_E1.0610.42
198_V294_R1.0570.41
382_I479_L1.0560.41
88_C391_E1.0550.41
79_G128_F1.0540.41
487_F491_Y1.0540.41
119_E178_A1.0520.41
332_M486_T1.0510.41
130_G137_P1.0480.40
92_A230_S1.0480.40
175_Y179_F1.0450.40
177_V245_L1.0440.40
314_N481_H1.0390.40
395_M440_L1.0380.39
123_M273_Q1.0380.39
116_T119_E1.0350.39
215_A449_T1.0350.39
432_I500_A1.0320.39
405_T454_T1.0320.39
324_L477_A1.0310.39
231_A338_M1.0290.39
192_Y357_V1.0290.39
271_I474_F1.0250.38
380_R452_G1.0240.38
137_P164_L1.0230.38
454_T483_T1.0220.38
364_L488_Y1.0210.38
344_G433_A1.0210.38
331_Y407_A1.0200.38
198_V362_G1.0170.38
85_G321_F1.0140.37
167_Y352_L1.0120.37
351_H430_L1.0120.37
94_K329_L1.0110.37
328_Q387_G1.0090.37
206_M348_A1.0010.36
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1pw4A10.72741000.767Contact Map
4j05A20.66841000.782Contact Map
3wdoA10.78121000.785Contact Map
4pypA10.71531000.787Contact Map
4zw9A101000.791Contact Map
2gfpA20.64581000.792Contact Map
3o7qA10.67191000.796Contact Map
4ldsA20.69971000.807Contact Map
4oh3A20.75171000.808Contact Map
4gc0A10.74131000.812Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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