| CrTIG |
| ID: | 1486538082 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 481 (416) | ||
| Sequences: | 3265 (2050.6) | ||
| Seq/Len: | 7.849 | ||
| Nf(neff/√len): | 100.5 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 7.849).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 242_S | 268_K | 3.997 | 1.00 |
| 237_M | 243_R | 3.834 | 1.00 |
| 173_R | 176_R | 3.585 | 1.00 |
| 246_N | 262_P | 3.511 | 1.00 |
| 152_E | 458_K | 3.389 | 1.00 |
| 218_R | 270_H | 3.335 | 1.00 |
| 190_L | 223_T | 3.210 | 1.00 |
| 308_A | 312_V | 2.920 | 1.00 |
| 237_M | 269_V | 2.883 | 1.00 |
| 91_A | 132_F | 2.810 | 1.00 |
| 203_T | 266_A | 2.780 | 1.00 |
| 412_A | 448_T | 2.774 | 1.00 |
| 54_Q | 60_A | 2.727 | 1.00 |
| 182_S | 276_T | 2.720 | 1.00 |
| 399_D | 403_R | 2.700 | 1.00 |
| 244_T | 264_K | 2.698 | 1.00 |
| 143_V | 332_D | 2.691 | 1.00 |
| 399_D | 402_V | 2.681 | 1.00 |
| 169_D | 173_R | 2.643 | 1.00 |
| 239_A | 272_I | 2.623 | 1.00 |
| 201_E | 215_T | 2.596 | 1.00 |
| 201_E | 266_A | 2.590 | 1.00 |
| 325_K | 389_D | 2.562 | 1.00 |
| 338_P | 342_E | 2.551 | 1.00 |
| 326_A | 329_D | 2.489 | 1.00 |
| 301_E | 305_A | 2.476 | 1.00 |
| 183_R | 271_E | 2.435 | 1.00 |
| 256_E | 259_Q | 2.394 | 1.00 |
| 30_T | 129_E | 2.380 | 1.00 |
| 246_N | 264_K | 2.349 | 1.00 |
| 201_E | 210_A | 2.342 | 1.00 |
| 416_E | 419_L | 2.318 | 1.00 |
| 238_K | 241_E | 2.315 | 1.00 |
| 166_A | 170_D | 2.304 | 1.00 |
| 302_A | 305_A | 2.218 | 1.00 |
| 388_E | 392_K | 2.200 | 1.00 |
| 304_I | 308_A | 2.177 | 1.00 |
| 165_A | 304_I | 2.174 | 1.00 |
| 171_L | 174_Q | 2.163 | 1.00 |
| 197_V | 270_H | 2.151 | 1.00 |
| 192_L | 232_S | 2.132 | 1.00 |
| 346_E | 380_I | 2.083 | 1.00 |
| 166_A | 169_D | 2.076 | 1.00 |
| 167_A | 311_R | 2.073 | 1.00 |
| 90_E | 94_K | 2.061 | 1.00 |
| 169_D | 300_R | 2.054 | 1.00 |
| 191_Q | 274_E | 2.053 | 1.00 |
| 26_S | 133_R | 2.051 | 1.00 |
| 244_T | 266_A | 2.044 | 1.00 |
| 378_E | 381_R | 2.029 | 1.00 |
| 199_D | 270_H | 2.023 | 1.00 |
| 149_E | 456_N | 2.015 | 1.00 |
| 297_K | 301_E | 2.004 | 1.00 |
| 177_K | 275_W | 1.970 | 1.00 |
| 384_K | 388_E | 1.964 | 1.00 |
| 248_S | 262_P | 1.957 | 1.00 |
| 164_D | 167_A | 1.948 | 1.00 |
| 305_A | 309_M | 1.923 | 1.00 |
| 189_G | 238_K | 1.851 | 1.00 |
| 452_W | 456_N | 1.847 | 1.00 |
| 305_A | 308_A | 1.835 | 1.00 |
| 125_D | 128_K | 1.814 | 1.00 |
| 242_S | 266_A | 1.798 | 1.00 |
| 173_R | 177_K | 1.796 | 1.00 |
| 298_E | 301_E | 1.793 | 1.00 |
| 94_K | 97_E | 1.772 | 1.00 |
| 190_L | 196_L | 1.756 | 1.00 |
| 30_T | 131_F | 1.736 | 1.00 |
| 35_T | 38_D | 1.731 | 1.00 |
| 285_V | 296_A | 1.704 | 1.00 |
| 197_V | 271_E | 1.702 | 1.00 |
| 37_K | 41_K | 1.700 | 1.00 |
| 203_T | 210_A | 1.686 | 1.00 |
| 176_R | 280_F | 1.683 | 1.00 |
| 154_T | 322_A | 1.677 | 1.00 |
| 404_K | 452_W | 1.675 | 1.00 |
| 318_A | 322_A | 1.672 | 1.00 |
| 198_I | 267_A | 1.665 | 1.00 |
| 350_Q | 353_L | 1.661 | 1.00 |
| 359_D | 362_G | 1.655 | 1.00 |
| 205_K | 261_M | 1.629 | 1.00 |
| 24_S | 137_D | 1.607 | 1.00 |
| 170_D | 174_Q | 1.598 | 1.00 |
| 410_D | 441_R | 1.594 | 1.00 |
| 197_V | 220_S | 1.578 | 1.00 |
| 381_R | 384_K | 1.568 | 1.00 |
| 312_V | 316_D | 1.566 | 1.00 |
| 346_E | 350_Q | 1.562 | 1.00 |
| 176_R | 277_L | 1.552 | 1.00 |
| 416_E | 447_V | 1.541 | 1.00 |
| 175_Y | 299_M | 1.540 | 1.00 |
| 190_L | 269_V | 1.534 | 1.00 |
| 408_L | 445_K | 1.521 | 1.00 |
| 325_K | 329_D | 1.498 | 0.99 |
| 210_A | 215_T | 1.494 | 0.99 |
| 418_S | 423_Q | 1.478 | 0.99 |
| 192_L | 235_L | 1.474 | 0.99 |
| 415_A | 418_S | 1.464 | 0.99 |
| 193_G | 222_D | 1.461 | 0.99 |
| 204_S | 261_M | 1.455 | 0.99 |
| 282_D | 285_V | 1.446 | 0.99 |
| 296_A | 300_R | 1.444 | 0.99 |
| 24_S | 135_M | 1.443 | 0.99 |
| 137_D | 339_R | 1.440 | 0.99 |
| 32_P | 35_T | 1.436 | 0.99 |
| 172_I | 176_R | 1.408 | 0.99 |
| 295_S | 298_E | 1.408 | 0.99 |
| 338_P | 384_K | 1.407 | 0.99 |
| 282_D | 286_A | 1.401 | 0.99 |
| 170_D | 173_R | 1.396 | 0.99 |
| 224_E | 231_T | 1.378 | 0.99 |
| 234_M | 237_M | 1.374 | 0.99 |
| 45_K | 48_E | 1.371 | 0.99 |
| 55_L | 64_L | 1.371 | 0.99 |
| 297_K | 300_R | 1.371 | 0.99 |
| 27_A | 95_G | 1.363 | 0.99 |
| 203_T | 264_K | 1.349 | 0.99 |
| 321_D | 325_K | 1.338 | 0.99 |
| 326_A | 330_A | 1.338 | 0.99 |
| 385_K | 388_E | 1.337 | 0.99 |
| 408_L | 444_V | 1.336 | 0.99 |
| 148_L | 404_K | 1.330 | 0.99 |
| 309_M | 313_T | 1.323 | 0.98 |
| 451_E | 455_D | 1.321 | 0.98 |
| 411_E | 415_A | 1.318 | 0.98 |
| 350_Q | 354_L | 1.301 | 0.98 |
| 310_Q | 314_E | 1.294 | 0.98 |
| 34_S | 37_K | 1.285 | 0.98 |
| 415_A | 419_L | 1.278 | 0.98 |
| 414_E | 418_S | 1.272 | 0.98 |
| 67_S | 70_I | 1.272 | 0.98 |
| 344_L | 390_Q | 1.270 | 0.98 |
| 204_S | 263_V | 1.266 | 0.98 |
| 172_I | 296_A | 1.262 | 0.98 |
| 410_D | 414_E | 1.261 | 0.98 |
| 184_V | 191_Q | 1.258 | 0.98 |
| 377_A | 381_R | 1.243 | 0.97 |
| 29_I | 132_F | 1.241 | 0.97 |
| 205_K | 263_V | 1.239 | 0.97 |
| 181_F | 195_T | 1.235 | 0.97 |
| 239_A | 271_E | 1.234 | 0.97 |
| 198_I | 234_M | 1.232 | 0.97 |
| 401_W | 449_V | 1.229 | 0.97 |
| 89_L | 93_P | 1.229 | 0.97 |
| 195_T | 222_D | 1.224 | 0.97 |
| 280_F | 296_A | 1.222 | 0.97 |
| 41_K | 45_K | 1.220 | 0.97 |
| 371_A | 378_E | 1.216 | 0.97 |
| 285_V | 299_M | 1.215 | 0.97 |
| 180_G | 275_W | 1.208 | 0.97 |
| 152_E | 326_A | 1.208 | 0.97 |
| 66_L | 70_I | 1.201 | 0.97 |
| 412_A | 415_A | 1.197 | 0.97 |
| 243_R | 269_V | 1.195 | 0.97 |
| 146_T | 331_L | 1.191 | 0.96 |
| 67_S | 71_Q | 1.184 | 0.96 |
| 294_G | 298_E | 1.169 | 0.96 |
| 229_G | 232_S | 1.167 | 0.96 |
| 197_V | 273_F | 1.166 | 0.96 |
| 409_E | 412_A | 1.158 | 0.96 |
| 199_D | 268_K | 1.156 | 0.96 |
| 143_V | 403_R | 1.152 | 0.95 |
| 147_P | 150_E | 1.150 | 0.95 |
| 167_A | 170_D | 1.140 | 0.95 |
| 327_V | 396_A | 1.139 | 0.95 |
| 139_A | 398_D | 1.129 | 0.95 |
| 316_D | 454_K | 1.126 | 0.95 |
| 406_L | 452_W | 1.125 | 0.95 |
| 64_L | 69_V | 1.124 | 0.95 |
| 248_S | 251_E | 1.123 | 0.95 |
| 237_M | 241_E | 1.119 | 0.94 |
| 28_I | 131_F | 1.118 | 0.94 |
| 28_I | 133_R | 1.114 | 0.94 |
| 407_V | 441_R | 1.107 | 0.94 |
| 233_G | 245_F | 1.093 | 0.93 |
| 38_D | 42_R | 1.093 | 0.93 |
| 400_I | 449_V | 1.088 | 0.93 |
| 142_V | 399_D | 1.087 | 0.93 |
| 196_L | 272_I | 1.086 | 0.93 |
| 301_E | 304_I | 1.085 | 0.93 |
| 184_V | 188_R | 1.081 | 0.93 |
| 351_A | 355_Q | 1.080 | 0.93 |
| 222_D | 275_W | 1.080 | 0.93 |
| 316_D | 451_E | 1.079 | 0.93 |
| 22_T | 334_P | 1.073 | 0.93 |
| 302_A | 306_S | 1.073 | 0.93 |
| 234_M | 243_R | 1.064 | 0.92 |
| 179_H | 288_Q | 1.062 | 0.92 |
| 51_V | 55_L | 1.062 | 0.92 |
| 42_R | 86_E | 1.049 | 0.92 |
| 149_E | 404_K | 1.049 | 0.92 |
| 198_I | 269_V | 1.041 | 0.91 |
| 199_D | 217_K | 1.038 | 0.91 |
| 165_A | 169_D | 1.034 | 0.91 |
| 184_V | 274_E | 1.033 | 0.91 |
| 195_T | 273_F | 1.032 | 0.91 |
| 413_V | 441_R | 1.030 | 0.91 |
| 172_I | 285_V | 1.030 | 0.91 |
| 84_L | 130_F | 1.029 | 0.90 |
| 92_L | 136_F | 1.023 | 0.90 |
| 322_A | 326_A | 1.023 | 0.90 |
| 26_S | 135_M | 1.017 | 0.90 |
| 283_E | 286_A | 1.011 | 0.89 |
| 171_L | 310_Q | 1.008 | 0.89 |
| 358_E | 362_G | 1.006 | 0.89 |
| 43_G | 82_F | 1.003 | 0.89 |
| 165_A | 300_R | 1.001 | 0.89 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1w26A | 2 | 0.8836 | 100 | 0.232 | Contact Map |
| 3gtyX | 1 | 0.8399 | 100 | 0.268 | Contact Map |
| 1t11A | 2 | 0.7734 | 100 | 0.282 | Contact Map |
| 1zxjA | 4 | 0.4012 | 99.9 | 0.74 | Contact Map |
| 1p9yA | 2 | 0.2432 | 99.9 | 0.742 | Contact Map |
| 2d3o1 | 1 | 0.2079 | 99.9 | 0.756 | Contact Map |
| 2nscA | 1 | 0.2266 | 99.9 | 0.766 | Contact Map |
| 2nsaA | 1 | 0.3347 | 99.9 | 0.774 | Contact Map |
| 1hxvA | 1 | 0.1726 | 99.4 | 0.857 | Contact Map |
| 3prbA | 2 | 0.3015 | 99.3 | 0.86 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)