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OPENSEQ.org

1086C

ID: 1472854316 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 849 (849)
Sequences: 6086 (374.1)
Seq/Len: 7.168
Nf(neff/√len): 12.8

Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 7.168).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
272_T437_L5.1951.00
618_D622_N3.8771.00
488_K593_A3.7151.00
318_H322_N3.4601.00
336_E339_K3.2331.00
644_K648_A3.2201.00
818_R822_I3.0841.00
92_K264_E2.9541.00
347_K451_T2.8431.00
553_Q615_M2.8431.00
733_R744_D2.6931.00
200_D366_R2.5671.00
219_N229_R2.4871.00
313_N403_K2.3811.00
282_N325_K2.3261.00
109_E191_T2.3261.00
320_N324_S2.2621.00
264_E267_T2.2581.00
57_E60_V2.2201.00
712_G722_E2.2171.00
7_W18_I2.1671.00
817_E822_I2.1571.00
790_G793_V2.1531.00
297_R302_Q2.1481.00
616_Q619_R2.1471.00
501_I505_F2.1331.00
627_T630_R2.1321.00
44_K94_D2.1221.00
18_I22_W2.0891.00
145_F160_A2.0791.00
606_N610_G2.0771.00
708_P712_G2.0651.00
14_L18_I2.0631.00
347_K453_T2.0221.00
15_I21_F1.9951.00
220_K256_E1.9871.00
18_I21_F1.9751.00
221_T257_E1.9341.00
80_V218_N1.9251.00
41_K622_N1.9021.00
334_G382_Y1.8861.00
258_E336_E1.8661.00
153_D181_R1.8621.00
645_D648_A1.8341.00
266_L340_H1.8221.00
212_F276_H1.8071.00
12_Q15_I1.8031.00
22_W26_M1.7891.00
409_N416_R1.7831.00
187_T191_T1.7541.00
294_K306_A1.7391.00
776_L780_Q1.7391.00
741_L744_D1.7371.00
504_V648_A1.7191.00
42_E615_M1.7131.00
446_N449_N1.7131.00
615_M618_D1.7051.00
15_I22_W1.7011.00
289_N426_E1.7001.00
686_A744_D1.6891.00
184_N189_A1.6821.00
259_I266_L1.6811.00
4_R10_W1.6721.00
87_N227_P1.6691.00
14_L21_F1.6681.00
810_T814_A1.6671.00
826_V830_C1.6661.00
60_V197_V1.6511.00
164_K184_N1.6431.00
297_R303_T1.6361.00
776_L779_L1.6351.00
711_R717_G1.6301.00
709_N712_G1.6221.00
778_G781_R1.6141.00
150_E184_N1.6101.00
742_V761_I1.6081.00
264_E268_N1.6061.00
808_I811_I1.6021.00
842_Q845_E1.6011.00
21_F26_M1.5981.00
830_C834_R1.5981.00
550_H553_Q1.5941.00
806_S810_T1.5891.00
841_R845_E1.5881.00
777_K780_Q1.5831.00
732_A744_D1.5691.00
775_S778_G1.5651.00
221_T229_R1.5631.00
316_Q322_N1.5611.00
845_E848_L1.5601.00
726_Q789_L1.5551.00
711_R729_D1.5461.00
811_I817_E1.5350.99
839_R845_E1.5310.99
551_M632_L1.5300.99
41_K618_D1.5250.99
843_G848_L1.5230.99
168_V181_R1.5150.99
798_L802_K1.5140.99
774_S779_L1.5130.99
60_V63_V1.5100.99
6_I18_I1.5050.99
482_V632_L1.5040.99
221_T225_T1.5000.99
791_S794_Q1.4990.99
774_S778_G1.4980.99
777_K781_R1.4970.99
282_N286_T1.4870.99
684_I691_V1.4850.99
818_R826_V1.4850.99
10_W14_L1.4840.99
841_R848_L1.4800.99
839_R842_Q1.4760.99
794_Q818_R1.4710.99
707_T742_V1.4710.99
150_E156_H1.4650.99
711_R727_D1.4640.99
785_I794_Q1.4610.99
175_S178_G1.4600.99
279_E332_K1.4590.99
809_D817_E1.4560.99
295_S309_D1.4490.99
318_H324_S1.4430.99
321_I350_P1.4410.99
318_H399_T1.4410.99
356_L416_R1.4370.99
259_I329_T1.4250.99
708_P722_E1.4190.99
775_S779_L1.4190.99
18_I23_I1.4160.99
657_N669_K1.4160.99
829_I832_A1.4120.99
259_I276_H1.4100.99
826_V829_I1.4090.99
781_R794_Q1.4040.99
781_R785_I1.4010.99
691_V718_G1.4000.99
366_R428_T1.3930.99
21_F25_N1.3910.99
416_R553_Q1.3890.99
147_A158_V1.3860.99
191_T409_N1.3830.99
770_L793_V1.3810.99
641_K644_K1.3790.99
212_F476_E1.3780.99
574_K632_L1.3690.99
6_I10_W1.3680.99
12_Q21_F1.3630.99
268_N460_D1.3600.98
744_D750_C1.3590.98
844_F849_L1.3480.98
41_K478_K1.3470.98
259_I334_G1.3440.98
41_K44_K1.3440.98
279_E325_K1.3440.98
783_W787_R1.3290.98
356_L360_T1.3290.98
842_Q848_L1.3290.98
8_K12_Q1.3280.98
637_N644_K1.3210.98
6_I14_L1.3150.98
284_V430_N1.3090.98
713_P729_D1.3080.98
710_P713_P1.3060.98
220_K257_E1.3040.98
303_T684_I1.3030.98
341_F344_K1.3020.98
109_E187_T1.2980.98
338_A382_Y1.2980.98
442_G452_E1.2970.98
616_Q620_E1.2960.98
278_N332_K1.2920.98
756_L759_D1.2900.98
189_A359_T1.2880.98
321_I553_Q1.2840.98
15_I25_N1.2820.98
709_N742_V1.2820.98
356_L408_I1.2810.98
761_I793_V1.2810.98
726_Q732_A1.2800.98
6_I21_F1.2780.97
686_A733_R1.2770.97
569_I574_K1.2740.97
774_S777_K1.2730.97
767_T794_Q1.2700.97
413_E416_R1.2690.97
443_G452_E1.2690.97
769_E772_G1.2660.97
840_I848_L1.2630.97
221_T258_E1.2620.97
338_A380_G1.2590.97
14_L23_I1.2590.97
776_L781_R1.2570.97
323_E396_G1.2570.97
334_G380_G1.2550.97
830_C833_I1.2540.97
780_Q793_V1.2470.97
322_N399_T1.2460.97
768_V771_L1.2440.97
336_E413_E1.2390.97
709_N713_P1.2380.97
316_Q320_N1.2360.97
838_R841_R1.2340.97
662_S669_K1.2340.97
109_E158_V1.2320.97
578_L588_L1.2290.97
742_V793_V1.2240.96
7_W23_I1.2210.96
622_N627_T1.2210.96
158_V189_A1.2180.96
707_T718_G1.2150.96
15_I26_M1.2140.96
722_E811_I1.2140.96
839_R848_L1.2120.96
18_I717_G1.2090.96
14_L17_S1.2090.96
19_L24_G1.2080.96
744_D748_S1.2030.96
588_L640_E1.1990.96
794_Q817_E1.1980.96
775_S780_Q1.1970.96
405_K408_I1.1960.96
150_E189_A1.1940.96
522_T531_L1.1930.96
708_P817_E1.1920.96
745_D754_Y1.1920.96
840_I843_G1.1910.96
742_V760_F1.1880.96
9_N835_N1.1870.96
127_T654_N1.1850.96
13_W16_W1.1830.96
756_L769_E1.1780.95
279_E328_N1.1770.95
268_N375_S1.1750.95
19_L60_V1.1710.95
739_L748_S1.1670.95
277_L336_E1.1670.95
4_R8_K1.1660.95
298_I302_Q1.1650.95
780_Q785_I1.1650.95
755_H771_L1.1640.95
811_I835_N1.1630.95
321_I329_T1.1620.95
504_V553_Q1.1620.95
7_W11_P1.1620.95
837_P841_R1.1590.95
132_N137_S1.1580.95
97_E653_K1.1570.95
202_I205_H1.1560.95
22_W25_N1.1560.95
707_T722_E1.1550.95
21_F27_E1.1550.95
55_A60_V1.1540.95
618_D621_I1.1520.95
293_R309_D1.1510.95
15_I798_L1.1490.95
553_Q569_I1.1480.94
653_K660_D1.1450.94
718_G781_R1.1440.94
713_P717_G1.1440.94
258_E278_N1.1420.94
424_E428_T1.1420.94
322_N501_I1.1420.94
14_L771_L1.1410.94
282_N430_N1.1400.94
12_Q22_W1.1390.94
543_R553_Q1.1360.94
476_E632_L1.1340.94
272_T277_L1.1290.94
10_W18_I1.1280.94
80_V615_M1.1280.94
630_R634_D1.1270.94
839_R843_G1.1240.94
837_P842_Q1.1240.94
12_Q19_L1.1230.94
212_F641_K1.1220.94
457_G460_D1.1200.93
736_S742_V1.1180.93
18_I789_L1.1170.93
814_A826_V1.1140.93
795_Y799_E1.1140.93
707_T736_S1.1100.93
101_E160_A1.1080.93
302_Q493_E1.1060.93
173_N179_E1.1060.93
605_Q616_Q1.1040.93
13_W802_K1.1030.93
4_R25_N1.1000.93
148_T153_D1.0990.93
10_W23_I1.0970.93
771_L781_R1.0950.92
645_D684_I1.0950.92
308_G424_E1.0950.92
8_K18_I1.0950.92
731_S735_A1.0930.92
569_I618_D1.0930.92
798_L846_T1.0900.92
770_L785_I1.0890.92
846_T849_L1.0890.92
709_N726_Q1.0870.92
8_K24_G1.0850.92
732_A736_S1.0850.92
428_T478_K1.0840.92
618_D627_T1.0830.92
709_N717_G1.0830.92
733_R740_T1.0830.92
645_D651_S1.0820.92
402_C411_W1.0800.92
705_T718_G1.0800.92
616_Q654_N1.0790.92
409_N457_G1.0790.92
827_Q830_C1.0790.92
18_I709_N1.0770.92
6_I11_P1.0770.92
709_N761_I1.0740.91
390_T395_N1.0740.91
440_R452_E1.0720.91
799_E807_L1.0720.91
22_W27_E1.0710.91
313_N322_N1.0710.91
11_P23_I1.0680.91
796_W804_A1.0670.91
304_F309_D1.0650.91
748_S798_L1.0650.91
160_A618_D1.0640.91
747_R751_L1.0640.91
383_R386_T1.0620.91
22_W708_P1.0610.91
709_N767_T1.0580.91
709_N722_E1.0560.91
736_S748_S1.0540.90
44_K767_T1.0510.90
184_N416_R1.0500.90
633_E640_E1.0490.90
726_Q761_I1.0470.90
844_F847_A1.0460.90
13_W17_S1.0450.90
553_Q618_D1.0450.90
711_R718_G1.0440.90
785_I817_E1.0400.90
218_N221_T1.0380.90
460_D662_S1.0380.90
44_K618_D1.0370.89
7_W12_Q1.0360.89
297_R304_F1.0340.89
12_Q25_N1.0340.89
160_A413_E1.0330.89
277_L437_L1.0320.89
181_R410_M1.0310.89
6_I9_N1.0300.89
257_E278_N1.0300.89
359_T493_E1.0290.89
4_R11_P1.0290.89
773_R781_R1.0280.89
499_V588_L1.0260.89
406_Q414_V1.0250.89
685_F688_L1.0250.89
219_N225_T1.0240.89
158_V191_T1.0240.89
2_R785_I1.0230.89
746_L754_Y1.0230.89
785_I818_R1.0220.89
151_L618_D1.0210.88
408_I710_P1.0210.88
57_E109_E1.0200.88
707_T712_G1.0200.88
747_R755_H1.0190.88
426_E430_N1.0190.88
7_W22_W1.0180.88
148_T162_F1.0180.88
390_T393_S1.0180.88
2_R607_E1.0180.88
229_R343_S1.0160.88
610_G684_I1.0150.88
36_G40_W1.0150.88
710_P722_E1.0140.88
14_L722_E1.0130.88
709_N817_E1.0110.88
41_K57_E1.0110.88
809_D818_R1.0100.88
158_V187_T1.0100.88
793_V798_L1.0090.88
189_A424_E1.0080.88
342_P345_T1.0070.88
755_H772_G1.0060.87
756_L771_L1.0050.87
7_W21_F1.0040.87
152_K159_H1.0040.87
836_I840_I1.0030.87
160_A553_Q1.0030.87
164_K183_I1.0030.87
384_N389_H1.0020.87
707_T761_I1.0010.87
338_A343_S1.0010.87
804_A807_L1.0010.87
676_G679_I1.0000.87
411_W419_Y1.0000.87
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4tvpG10.53121000.435Contact Map
4r4hA10.39691000.488Contact Map
4jzzA10.39461000.563Contact Map
3lqaG10.34041000.63Contact Map
2bf1A10.34631000.64Contact Map
4i3sG10.21081000.82Contact Map
4tvpB10.14841000.822Contact Map
3tygA10.18261000.843Contact Map
1qbzA30.14131000.859Contact Map
4jpkA10.18851000.872Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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