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OPENSEQ.org

2G67

ID: 1463672225 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 886 (874)
Sequences: 1164 (404.7)
Seq/Len: 1.332
Nf(neff/√len): 13.7

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.332).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
151_A186_F5.3511.00
624_I691_T4.7151.00
713_I758_V4.4831.00
346_A349_A4.1921.00
275_I293_M4.1311.00
374_K378_E4.1231.00
163_D438_Y3.7991.00
786_P789_A3.7231.00
618_Q684_N3.6461.00
452_H461_Y3.3641.00
23_V35_L3.2071.00
692_L733_S3.1591.00
628_S692_L3.0761.00
329_D333_V2.8651.00
628_S733_S2.8611.00
708_G787_Y2.8191.00
662_Y716_L2.8041.00
662_Y739_R2.7511.00
201_A255_F2.7241.00
765_A769_Q2.7121.00
788_I811_V2.6641.00
740_E845_A2.6191.00
7_D13_T2.6041.00
223_T254_V2.6021.00
854_G858_K2.5171.00
110_F118_D2.5061.00
429_S432_D2.5061.00
675_E680_E2.4851.00
749_Y874_F2.4641.00
739_R755_V2.4621.00
208_L588_N2.4601.00
443_E452_H2.4571.00
233_M238_S2.4221.00
850_V867_V2.3700.99
744_I874_F2.3580.99
765_A809_E2.3310.99
651_T804_M2.3190.99
582_A619_A2.3190.99
313_L344_T2.2930.99
497_A663_A2.2920.99
857_A867_V2.2900.99
774_W778_H2.2780.99
207_F592_M2.2130.99
206_K577_S2.1980.99
785_V793_N2.1850.99
109_S143_I2.1760.99
297_R360_N2.1710.99
307_S347_L2.1650.99
433_I437_P2.1610.99
768_G812_R2.1520.99
728_L738_V2.1440.99
454_Q457_K2.1400.99
726_V852_A2.1340.98
607_I611_C2.1030.98
322_Y389_H2.1020.98
774_W784_R2.1000.98
611_C652_I2.0920.98
498_F502_L2.0200.98
672_D675_E2.0010.97
64_P67_E1.9980.97
482_G486_E1.9880.97
216_L590_L1.9740.97
744_I849_V1.9240.97
444_G449_T1.9000.96
762_T807_F1.8960.96
584_S588_N1.8950.96
738_V800_S1.8940.96
470_T589_N1.8790.96
233_M239_K1.8720.96
869_D873_K1.8710.96
648_Q651_T1.8690.96
866_V869_D1.8450.95
406_A409_V1.8440.95
676_R683_E1.8290.95
326_K333_V1.8260.95
857_A862_I1.8050.95
7_D10_P1.8030.95
769_Q809_E1.7950.94
740_E743_E1.7920.94
7_D34_Y1.7870.94
21_E42_E1.7850.94
666_V689_I1.7830.94
257_I598_Y1.7650.94
116_I373_F1.7600.94
379_T382_K1.7590.94
541_N544_Q1.7560.93
578_W582_A1.7420.93
288_N384_T1.7320.93
813_T816_P1.7270.93
452_H456_Q1.7060.92
730_G738_V1.6990.92
506_L535_I1.6870.92
80_R447_E1.6830.91
307_S343_E1.6440.90
634_N641_E1.6390.90
575_G611_C1.6330.90
126_R132_D1.6160.89
746_A752_G1.6100.89
175_S183_M1.6030.89
273_K276_N1.6010.88
649_S657_S1.6010.88
352_T355_Q1.5910.88
819_D859_R1.5890.88
769_Q805_K1.5880.88
10_P13_T1.5830.88
744_I749_Y1.5820.88
60_I315_N1.5710.87
725_K754_D1.5680.87
307_S341_Y1.5680.87
312_Q316_E1.5660.87
243_T281_I1.5470.86
413_N416_G1.5420.86
672_D710_R1.5380.86
611_C623_L1.5360.86
115_T394_Y1.5350.86
68_Q296_S1.5310.85
800_S848_V1.5300.85
313_L317_T1.5270.85
724_G750_G1.5270.85
13_T34_Y1.5210.85
656_I676_R1.5160.85
442_P448_H1.5110.84
201_A226_A1.5100.84
206_K567_E1.5090.84
200_G579_L1.5090.84
228_L238_S1.5070.84
514_R590_L1.5050.84
728_L800_S1.4960.83
727_Q795_A1.4930.83
501_A666_V1.4880.83
649_S762_T1.4790.83
484_L504_V1.4780.82
121_F147_V1.4690.82
566_Q641_E1.4580.81
126_R221_K1.4570.81
335_E345_A1.4530.81
539_S544_Q1.4510.81
65_V68_Q1.4450.80
705_A709_I1.4400.80
717_E739_R1.4330.80
290_I386_I1.4310.80
34_Y38_Q1.4310.80
787_Y791_V1.4250.79
865_K869_D1.4240.79
321_D546_T1.4220.79
743_E747_K1.4210.79
115_T369_I1.4190.79
314_M337_F1.4180.79
611_C686_Y1.4160.79
453_A456_Q1.4140.78
474_E510_S1.4120.78
821_R855_E1.4080.78
60_I311_I1.4040.78
776_M847_Y1.4030.78
672_D676_R1.3980.77
141_G192_V1.3970.77
727_Q754_D1.3950.77
9_D13_T1.3950.77
748_D874_F1.3900.77
484_L663_A1.3870.77
128_R188_Q1.3870.77
717_E743_E1.3860.76
13_T31_R1.3830.76
668_V709_I1.3800.76
33_Q37_D1.3740.76
780_L868_A1.3710.75
414_M434_E1.3630.75
20_I39_L1.3610.75
24_I29_V1.3460.73
311_I315_N1.3440.73
849_V852_A1.3430.73
607_I652_I1.3420.73
744_I846_S1.3390.73
744_I748_D1.3350.73
506_L531_L1.3330.72
212_E218_D1.3300.72
672_D683_E1.3250.72
730_G735_L1.3220.72
839_H844_D1.3210.71
56_I280_G1.3190.71
501_A670_M1.3180.71
69_P371_A1.3120.71
74_N446_E1.3110.71
374_K377_Q1.3110.71
863_D866_V1.3040.70
531_L535_I1.2960.69
749_Y849_V1.2940.69
108_A396_M1.2930.69
59_Y389_H1.2910.69
428_V437_P1.2900.69
713_I791_V1.2840.68
713_I756_Y1.2670.67
511_I515_L1.2640.67
507_K534_Q1.2630.67
771_C775_N1.2620.66
719_I753_S1.2590.66
847_Y876_I1.2520.66
333_V356_I1.2480.65
84_A441_F1.2470.65
661_A698_M1.2460.65
354_E716_L1.2460.65
735_L755_V1.2450.65
748_D873_K1.2370.64
778_H871_I1.2350.64
202_I240_G1.2340.64
664_Y701_M1.2300.63
69_P374_K1.2300.63
304_K350_D1.2300.63
666_V738_V1.2280.63
137_V233_M1.2260.63
60_I302_L1.2260.63
797_A817_A1.2230.63
650_L762_T1.2230.63
614_A619_A1.2180.62
666_V691_T1.2130.62
313_L340_K1.2110.62
441_F448_H1.2110.62
228_L233_M1.2090.62
300_E347_L1.2050.61
869_D872_A1.2050.61
546_T566_Q1.2050.61
418_R433_I1.2030.61
45_K66_E1.2010.61
773_R842_E1.2000.61
778_H867_V1.1980.60
846_S874_F1.1980.60
297_R358_A1.1950.60
800_S843_V1.1920.60
223_T377_Q1.1920.60
324_T329_D1.1890.60
862_I866_V1.1890.60
593_I678_Y1.1880.60
115_T366_P1.1850.59
466_Q589_N1.1830.59
45_K48_V1.1770.58
511_I593_I1.1720.58
647_I804_M1.1720.58
805_K814_Y1.1720.58
808_A822_V1.1710.58
498_F624_I1.1700.58
56_I591_P1.1670.57
863_D869_D1.1670.57
812_R822_V1.1630.57
668_V701_M1.1630.57
839_H847_Y1.1570.56
84_A439_I1.1570.56
17_L38_Q1.1570.56
769_Q814_Y1.1540.56
400_A409_V1.1530.56
38_Q41_A1.1530.56
533_R555_Y1.1520.56
487_E663_A1.1520.56
771_C786_P1.1510.56
288_N375_K1.1510.56
109_S584_S1.1510.56
615_G828_F1.1490.56
523_A538_Y1.1470.56
716_L755_V1.1460.55
849_V853_L1.1440.55
821_R851_A1.1420.55
730_G734_I1.1420.55
448_H452_H1.1410.55
530_G554_A1.1390.55
867_V871_I1.1370.55
449_T453_A1.1360.54
24_I302_L1.1350.54
566_Q626_G1.1350.54
346_A350_D1.1340.54
440_T448_H1.1330.54
334_R353_D1.1300.54
49_N60_I1.1280.54
228_L285_A1.1260.53
170_H836_N1.1260.53
597_I624_I1.1250.53
798_V806_L1.1220.53
785_V789_A1.1220.53
55_G58_N1.1200.53
339_G345_A1.1190.53
71_Y77_L1.1170.53
199_I610_L1.1120.52
566_Q570_N1.1100.52
116_I369_I1.1090.52
103_L835_E1.1080.52
116_I256_V1.1080.52
408_Q811_V1.1050.51
128_R464_S1.1030.51
662_Y666_V1.1020.51
850_V871_I1.0990.51
749_Y873_K1.0990.51
43_A48_V1.0980.51
657_S762_T1.0970.51
340_K344_T1.0950.50
45_K58_N1.0940.50
94_L412_M1.0930.50
440_T452_H1.0910.50
144_S461_Y1.0900.50
45_K64_P1.0890.50
68_Q280_G1.0870.50
321_D340_K1.0860.49
71_Y75_L1.0820.49
71_Y123_H1.0800.49
825_T843_V1.0790.49
317_T325_F1.0790.49
331_A335_E1.0760.48
113_S143_I1.0700.48
418_R437_P1.0700.48
655_C686_Y1.0690.48
716_L739_R1.0680.48
181_K618_Q1.0640.47
715_K718_T1.0630.47
133_G167_Q1.0630.47
71_Y367_K1.0620.47
444_G448_H1.0610.47
673_G677_M1.0580.47
624_I692_L1.0570.47
487_E520_A1.0520.46
33_Q48_V1.0510.46
729_L758_V1.0510.46
432_D437_P1.0510.46
725_K752_G1.0500.46
199_I241_A1.0500.46
756_Y791_V1.0490.46
158_T161_Q1.0470.46
770_D784_R1.0460.45
858_K862_I1.0460.45
789_A816_P1.0450.45
438_Y546_T1.0410.45
294_W315_N1.0400.45
110_F114_A1.0380.45
499_V519_I1.0380.45
14_R42_E1.0370.45
68_Q355_Q1.0370.45
37_D58_N1.0350.44
327_S333_V1.0350.44
333_V357_W1.0340.44
108_A433_I1.0340.44
213_H560_E1.0330.44
761_F799_A1.0330.44
82_R115_T1.0330.44
512_K515_L1.0320.44
352_T647_I1.0310.44
77_L446_E1.0310.44
863_D874_F1.0310.44
514_R584_S1.0300.44
164_N557_K1.0300.44
288_N382_K1.0290.44
259_C387_L1.0270.44
453_A457_K1.0270.44
872_A875_N1.0260.43
325_F336_H1.0260.43
778_H781_E1.0250.43
326_K357_W1.0230.43
624_I689_I1.0230.43
321_D324_T1.0220.43
212_E219_T1.0220.43
68_Q71_Y1.0210.43
775_N778_H1.0200.43
202_I241_A1.0200.43
727_Q756_Y1.0190.43
58_N347_L1.0190.43
406_A413_N1.0180.43
11_I858_K1.0180.43
664_Y709_I1.0170.43
656_I685_V1.0160.42
654_N695_N1.0150.42
347_L662_Y1.0140.42
656_I683_E1.0140.42
711_K787_Y1.0120.42
116_I388_A1.0120.42
33_Q49_N1.0110.42
366_P369_I1.0100.42
719_I725_K1.0090.42
58_N315_N1.0090.42
30_E64_P1.0080.42
18_Q131_Q1.0080.42
567_E577_S1.0070.42
33_Q355_Q1.0060.41
127_A461_Y1.0040.41
604_F649_S1.0030.41
64_P446_E1.0030.41
850_V857_A1.0020.41
46_G805_K1.0020.41
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2qtcA20.90411000.425Contact Map
3ahcA10.80591000.712Contact Map
1r9jA20.7281000.785Contact Map
3rimA40.74271000.794Contact Map
2r8oA20.70771000.795Contact Map
1itzA30.71671000.8Contact Map
4c7vA10.71441000.801Contact Map
1gpuA20.72691000.802Contact Map
2e6kA40.70431000.806Contact Map
3uk1A20.72231000.807Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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