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OPENSEQ.org

catpasa

ID: 1461011470 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 994 (971)
Sequences: 7327 (4270.9)
Seq/Len: 7.546
Nf(neff/√len): 137.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 7.546).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
163_I221_G7.4721.00
26_P132_A5.0691.00
29_V33_L4.8421.00
151_V163_I4.4241.00
636_C675_C4.1601.00
36_Y147_P4.1181.00
346_S696_E4.0771.00
755_N819_R4.0261.00
28_Q32_H4.0171.00
616_D818_D4.0011.00
40_E143_R3.9371.00
365_K557_D3.9101.00
416_I594_V3.8311.00
175_V214_I3.7521.00
151_V221_G3.6481.00
514_V591_L3.5611.00
173_L219_A3.5041.00
689_E713_K3.4491.00
147_P226_T3.4111.00
662_P665_E3.3281.00
63_R67_L3.3071.00
663_L686_K3.2891.00
28_Q31_R3.2851.00
511_K568_D3.2731.00
163_I208_L3.2001.00
48_S51_E3.1951.00
667_R693_S3.1801.00
207_M224_A3.1521.00
33_L226_T3.1121.00
619_I747_V3.0611.00
358_T605_K3.0211.00
13_L222_I3.0201.00
719_A731_S2.9601.00
47_K55_E2.9241.00
659_D683_H2.9231.00
130_Y220_L2.9121.00
42_P228_V2.8911.00
175_V208_L2.8811.00
151_V160_P2.8451.00
946_L950_V2.7581.00
208_L221_G2.7561.00
606_E739_N2.7211.00
246_K254_D2.7161.00
31_R35_K2.7151.00
33_L38_H2.6241.00
329_K748_E2.6181.00
526_N590_D2.5951.00
204_K209_F2.5901.00
609_G613_L2.5621.00
566_T592_T2.5541.00
150_I166_L2.5171.00
538_T541_V2.5091.00
612_Q616_D2.4961.00
231_E234_K2.4951.00
620_R674_C2.4851.00
38_H144_D2.4831.00
126_M139_R2.4721.00
954_P961_A2.4711.00
73_F91_P2.4681.00
554_T598_G2.4551.00
613_L748_E2.4351.00
331_A742_T2.4241.00
633_I637_R2.4091.00
614_C744_V2.3981.00
495_S512_M2.3891.00
13_L20_E2.3761.00
49_L106_V2.3761.00
653_Y661_L2.3711.00
163_I224_A2.3531.00
482_E510_N2.3521.00
543_E547_S2.3491.00
565_A591_L2.3471.00
622_I691_L2.3041.00
153_V159_V2.3001.00
495_S514_V2.3001.00
275_N295_Y2.2911.00
913_L937_I2.2791.00
603_P639_I2.2781.00
211_G232_I2.2681.00
509_G568_D2.2511.00
329_K745_A2.2451.00
8_S11_E2.2431.00
692_Q713_K2.2371.00
146_V226_T2.2071.00
606_E609_G2.2041.00
550_K553_G2.1761.00
480_K498_C2.1561.00
130_Y152_E2.1241.00
414_A448_L2.1231.00
57_F102_A2.1171.00
12_C222_I2.1081.00
480_K499_S2.1041.00
174_R216_A2.0761.00
332_I736_A2.0661.00
545_I593_F2.0601.00
563_A593_F2.0511.00
30_K34_E2.0221.00
137_V152_E2.0221.00
103_I107_W2.0191.00
422_D481_K2.0171.00
13_L166_L2.0091.00
159_V214_I2.0071.00
493_S524_R2.0021.00
49_L53_V2.0011.00
173_L214_I2.0011.00
752_A812_P1.9981.00
167_S220_L1.9841.00
514_V521_V1.9821.00
141_K144_D1.9781.00
416_I513_F1.9681.00
38_H143_R1.9241.00
559_L598_G1.9101.00
527_Y592_T1.9101.00
30_K33_L1.9101.00
253_L315_I1.9061.00
499_S510_N1.9001.00
436_K443_T1.8991.00
152_E220_L1.8941.00
963_D966_Q1.8791.00
165_I208_L1.8741.00
561_C596_V1.8651.00
386_G389_Y1.8611.00
221_G224_A1.8581.00
130_Y137_V1.8511.00
495_S588_E1.8381.00
611_I641_I1.8301.00
165_I221_G1.8231.00
902_S944_H1.8211.00
895_E966_Q1.8171.00
494_M515_K1.8121.00
69_A95_L1.8081.00
66_L70_C1.8061.00
145_I151_V1.8011.00
364_C638_R1.7951.00
745_A748_E1.7461.00
567_R589_T1.7451.00
347_V622_I1.7301.00
412_E566_T1.7261.00
296_F300_V1.7241.00
554_T559_L1.7241.00
262_K266_L1.7221.00
333_V733_M1.7121.00
566_T594_V1.7011.00
31_R34_E1.7011.00
667_R690_Y1.6951.00
497_Y569_T1.6901.00
130_Y150_I1.6871.00
541_V544_K1.6811.00
982_E985_K1.6771.00
528_V541_V1.6761.00
441_T599_M1.6741.00
567_R590_D1.6701.00
126_M141_K1.6691.00
58_E63_R1.6671.00
711_L727_A1.6661.00
671_R694_Y1.6641.00
542_K546_L1.6621.00
128_K154_A1.6581.00
153_V214_I1.6561.00
128_K137_V1.6561.00
251_Q824_P1.6511.00
386_G393_G1.6481.00
624_I687_I1.6431.00
345_T716_I1.6411.00
606_E741_S1.6391.00
535_V541_V1.6301.00
145_I223_V1.6271.00
19_S22_T1.6141.00
153_V208_L1.6081.00
771_E799_T1.6051.00
150_I222_I1.5951.00
523_D542_K1.5931.00
552_W630_G1.5921.00
521_V525_C1.5881.00
245_D692_Q1.5841.00
666_Q686_K1.5841.00
13_L150_I1.5831.00
265_S269_V1.5821.00
16_F32_H1.5811.00
911_N914_N1.5791.00
43_A120_K1.5771.00
512_M569_T1.5741.00
165_I219_A1.5701.00
608_M612_Q1.5671.00
106_V110_R1.5601.00
25_T29_V1.5591.00
699_A718_I1.5581.00
539_G542_K1.5571.00
491_R588_E1.5561.00
363_V445_L1.5551.00
255_E259_Q1.5481.00
391_P434_Y1.5421.00
165_I191_T1.5391.00
609_G612_Q1.5341.00
829_I833_L1.5341.00
165_I168_I1.5261.00
545_I596_V1.5261.00
493_S588_E1.5231.00
47_K51_E1.5201.00
66_L98_L1.5170.99
564_L597_V1.5140.99
26_P138_Q1.5020.99
629_K654_T1.5000.99
272_W299_A1.4980.99
27_D31_R1.4950.99
25_T28_Q1.4920.99
53_V106_V1.4880.99
615_R643_G1.4820.99
325_R749_E1.4780.99
33_L144_D1.4780.99
155_V488_S1.4720.99
947_I957_F1.4710.99
692_Q715_E1.4600.99
794_W944_H1.4570.99
9_T166_L1.4550.99
547_S553_G1.4530.99
29_V38_H1.4460.99
25_T132_A1.4430.99
7_K15_Y1.4330.99
412_E415_T1.4290.99
716_I733_M1.4270.99
690_Y693_S1.4260.99
26_P30_K1.4200.99
134_R138_Q1.4140.99
654_T657_E1.4140.99
537_M545_I1.4050.99
629_K633_I1.4000.99
334_R731_S1.3990.99
13_L18_V1.3960.99
46_G117_E1.3920.99
482_E499_S1.3920.99
600_L638_R1.3810.99
661_L669_A1.3780.99
363_V448_L1.3780.99
625_T632_A1.3770.99
331_A746_A1.3750.99
204_K231_E1.3740.99
448_L597_V1.3700.99
498_C513_F1.3690.99
53_V57_F1.3650.99
492_K517_A1.3570.99
345_T699_A1.3570.99
174_R188_I1.3570.99
365_K554_T1.3550.99
158_K729_T1.3550.99
7_K11_E1.3540.99
636_C641_I1.3540.99
8_S12_C1.3510.99
349_C624_I1.3510.99
330_N736_A1.3500.99
688_V698_T1.3490.99
623_M675_C1.3480.99
177_Q189_K1.3480.99
902_S948_L1.3420.98
906_T941_M1.3400.98
140_I145_I1.3370.98
968_L972_K1.3370.98
527_Y536_P1.3360.98
330_N737_D1.3320.98
844_V907_I1.3260.98
552_W556_R1.3230.98
385_T396_L1.3220.98
982_E989_R1.3220.98
525_C565_A1.3190.98
20_E166_L1.3080.98
32_H36_Y1.3060.98
614_C621_V1.3060.98
161_A223_V1.3040.98
689_E693_S1.3040.98
114_N117_E1.3010.98
481_K484_T1.2940.98
497_Y512_M1.2910.98
389_Y393_G1.2900.98
556_R630_G1.2870.98
539_G543_E1.2870.98
613_L616_D1.2850.98
632_A677_A1.2840.98
164_R207_M1.2840.98
110_R114_N1.2800.98
614_C740_F1.2770.98
9_T12_C1.2760.98
53_V102_A1.2740.98
159_V208_L1.2730.98
703_D706_N1.2730.98
351_D355_T1.2710.98
653_Y658_F1.2660.98
166_L222_I1.2660.98
521_V565_A1.2650.98
945_F949_Y1.2640.98
848_T900_A1.2610.97
558_T634_A1.2590.97
418_A449_V1.2560.97
949_Y959_L1.2560.97
53_V103_I1.2550.97
71_I296_F1.2530.97
29_V32_H1.2530.97
491_R495_S1.2470.97
417_C597_V1.2450.97
365_K559_L1.2440.97
949_Y961_A1.2430.97
164_R222_I1.2430.97
330_N742_T1.2390.97
55_E58_E1.2360.97
946_L953_L1.2340.97
27_D30_K1.2340.97
324_R328_K1.2320.97
127_G142_A1.2280.97
511_K566_T1.2160.97
367_F554_T1.2060.96
56_Q105_G1.2040.96
624_I700_M1.2040.96
543_E546_L1.2040.96
363_V599_M1.2030.96
33_L36_Y1.2030.96
416_I564_L1.1990.96
676_F691_L1.1980.96
737_D742_T1.1980.96
443_T447_T1.1970.96
553_G557_D1.1960.96
633_I642_F1.1930.96
322_G753_I1.1930.96
623_M636_C1.1880.96
568_D592_T1.1880.96
528_V535_V1.1880.96
621_V740_F1.1830.96
697_I824_P1.1820.96
423_S446_T1.1820.96
10_E14_A1.1780.96
388_T393_G1.1780.96
421_N445_L1.1730.96
485_L495_S1.1720.96
954_P959_L1.1720.96
610_S741_S1.1690.96
964_L968_L1.1680.96
720_M723_G1.1640.95
73_F95_L1.1590.95
764_L804_A1.1580.95
768_N800_D1.1550.95
356_L639_I1.1550.95
607_V740_F1.1540.95
948_L959_L1.1480.95
367_F550_K1.1480.95
725_A728_K1.1480.95
92_F96_L1.1450.95
557_D638_R1.1440.95
771_E908_E1.1410.95
788_I958_K1.1400.95
934_L938_C1.1370.95
512_M591_L1.1370.95
40_E43_A1.1360.95
246_K251_Q1.1360.95
804_A808_G1.1330.94
349_C691_L1.1320.94
653_Y673_A1.1300.94
770_G845_G1.1280.94
445_L597_V1.1270.94
641_I675_C1.1220.94
128_K139_R1.1200.94
175_V219_A1.1200.94
823_S826_E1.1180.94
810_N813_D1.1150.94
629_K657_E1.1110.94
547_S552_W1.1100.94
896_P900_A1.1100.94
520_G524_R1.1040.93
366_M448_L1.1030.93
481_K498_C1.1020.93
140_I144_D1.1020.93
27_D132_A1.0990.93
512_M587_Y1.0960.93
899_M970_V1.0940.93
938_C942_S1.0930.93
128_K157_D1.0920.93
623_M632_A1.0910.93
382_F395_V1.0880.93
489_R492_K1.0850.93
238_Q682_S1.0800.92
334_R734_V1.0780.92
346_S691_L1.0770.92
983_I987_I1.0710.92
43_A117_E1.0710.92
51_E55_E1.0650.92
810_N916_L1.0640.92
60_L257_G1.0640.92
421_N446_T1.0620.92
679_V683_H1.0590.91
986_F989_R1.0580.91
225_T229_S1.0550.91
894_P897_M1.0540.91
799_T908_E1.0540.91
11_E14_A1.0490.91
447_T451_K1.0430.91
129_V138_Q1.0420.91
607_V741_S1.0390.90
697_I715_E1.0380.90
113_E334_R1.0370.90
658_F683_H1.0360.90
519_E628_N1.0360.90
142_A145_I1.0350.90
527_Y590_D1.0340.90
778_T786_A1.0340.90
3_A6_S1.0320.90
258_E262_K1.0320.90
981_D984_L1.0310.90
663_L689_E1.0300.90
442_E494_M1.0290.90
671_R696_E1.0270.90
329_K749_E1.0270.90
642_F675_C1.0260.90
688_V700_M1.0230.89
201_N204_K1.0210.89
908_E911_N1.0200.89
518_P551_E1.0190.89
96_L100_A1.0190.89
848_T903_V1.0180.89
921_S982_E1.0170.89
4_A16_F1.0170.89
363_V597_V1.0140.89
214_I219_A1.0130.89
665_E668_E1.0100.89
48_S54_I1.0080.89
718_I743_I1.0070.88
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3ar4A10.9981000.157Contact Map
3ixzA10.94371000.214Contact Map
2zxeA10.94271000.214Contact Map
1mhsA20.82291000.313Contact Map
3b8cA20.8341000.32Contact Map
4bbjA10.63281000.592Contact Map
4umwA10.58851000.599Contact Map
3j09A20.61671000.608Contact Map
3j08A20.62681000.613Contact Map
3a1cA20.27461000.87Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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