| G6PC |
| ID: | 1447171807 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 357 (347) | ||
| Sequences: | 431 (303.8) | ||
| Seq/Len: | 1.242 | ||
| Nf(neff/√len): | 16.3 |
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.242).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 119_H | 170_R | 2.342 | 0.99 |
| 170_R | 176_H | 2.040 | 0.97 |
| 117_S | 176_H | 1.876 | 0.95 |
| 274_A | 338_V | 1.845 | 0.95 |
| 119_H | 176_H | 1.809 | 0.94 |
| 34_S | 169_S | 1.699 | 0.91 |
| 118_G | 176_H | 1.598 | 0.87 |
| 76_K | 119_H | 1.565 | 0.86 |
| 76_K | 170_R | 1.554 | 0.85 |
| 81_G | 114_G | 1.553 | 0.85 |
| 270_G | 342_P | 1.478 | 0.81 |
| 128_Y | 192_A | 1.468 | 0.80 |
| 350_G | 353_H | 1.452 | 0.79 |
| 83_R | 170_R | 1.451 | 0.79 |
| 58_G | 72_N | 1.449 | 0.79 |
| 86_W | 177_F | 1.447 | 0.79 |
| 161_A | 211_L | 1.446 | 0.79 |
| 48_P | 77_W | 1.444 | 0.79 |
| 72_N | 118_G | 1.432 | 0.78 |
| 166_V | 169_S | 1.414 | 0.77 |
| 112_G | 178_P | 1.410 | 0.76 |
| 92_D | 354_K | 1.403 | 0.76 |
| 58_G | 130_M | 1.395 | 0.75 |
| 274_A | 336_A | 1.394 | 0.75 |
| 76_K | 176_H | 1.386 | 0.75 |
| 272_G | 337_S | 1.376 | 0.74 |
| 51_F | 55_E | 1.375 | 0.74 |
| 117_S | 170_R | 1.351 | 0.72 |
| 39_L | 177_F | 1.351 | 0.72 |
| 295_R | 298_S | 1.330 | 0.70 |
| 99_V | 352_P | 1.328 | 0.70 |
| 20_Q | 257_P | 1.326 | 0.70 |
| 48_P | 257_P | 1.313 | 0.69 |
| 176_H | 184_G | 1.296 | 0.67 |
| 298_S | 345_L | 1.292 | 0.67 |
| 37_A | 170_R | 1.290 | 0.67 |
| 94_Y | 100_P | 1.288 | 0.67 |
| 79_L | 115_S | 1.283 | 0.66 |
| 83_R | 117_S | 1.282 | 0.66 |
| 30_F | 166_V | 1.279 | 0.66 |
| 351_Q | 355_K | 1.272 | 0.65 |
| 37_A | 176_H | 1.267 | 0.65 |
| 87_W | 175_A | 1.265 | 0.65 |
| 98_S | 355_K | 1.259 | 0.64 |
| 189_I | 294_F | 1.247 | 0.63 |
| 51_F | 58_G | 1.246 | 0.63 |
| 114_G | 295_R | 1.244 | 0.63 |
| 254_D | 258_F | 1.240 | 0.62 |
| 65_A | 69_D | 1.233 | 0.62 |
| 20_Q | 48_P | 1.230 | 0.61 |
| 351_Q | 354_K | 1.229 | 0.61 |
| 83_R | 119_H | 1.224 | 0.61 |
| 119_H | 342_P | 1.221 | 0.61 |
| 133_S | 348_V | 1.218 | 0.60 |
| 38_D | 48_P | 1.213 | 0.60 |
| 30_F | 270_G | 1.212 | 0.60 |
| 112_G | 118_G | 1.212 | 0.60 |
| 95_S | 99_V | 1.211 | 0.60 |
| 352_P | 355_K | 1.210 | 0.59 |
| 99_V | 238_L | 1.206 | 0.59 |
| 45_V | 300_V | 1.202 | 0.59 |
| 349_L | 355_K | 1.194 | 0.58 |
| 332_V | 340_V | 1.190 | 0.58 |
| 118_G | 170_R | 1.189 | 0.57 |
| 119_H | 169_S | 1.187 | 0.57 |
| 37_A | 119_H | 1.186 | 0.57 |
| 167_C | 185_V | 1.179 | 0.57 |
| 110_E | 178_P | 1.177 | 0.56 |
| 92_D | 95_S | 1.176 | 0.56 |
| 41_N | 334_P | 1.173 | 0.56 |
| 299_I | 303_L | 1.170 | 0.56 |
| 334_P | 338_V | 1.170 | 0.56 |
| 169_S | 342_P | 1.169 | 0.56 |
| 50_W | 219_F | 1.163 | 0.55 |
| 350_G | 355_K | 1.160 | 0.55 |
| 270_G | 274_A | 1.158 | 0.54 |
| 68_G | 337_S | 1.153 | 0.54 |
| 115_S | 180_Q | 1.148 | 0.53 |
| 124_A | 166_V | 1.142 | 0.53 |
| 42_A | 69_D | 1.142 | 0.53 |
| 16_T | 114_G | 1.139 | 0.53 |
| 85_Y | 104_Q | 1.138 | 0.53 |
| 130_M | 305_L | 1.137 | 0.52 |
| 97_T | 140_G | 1.135 | 0.52 |
| 230_L | 254_D | 1.134 | 0.52 |
| 70_W | 112_G | 1.134 | 0.52 |
| 118_G | 127_Y | 1.134 | 0.52 |
| 86_W | 110_E | 1.133 | 0.52 |
| 85_Y | 118_G | 1.130 | 0.52 |
| 169_S | 176_H | 1.129 | 0.52 |
| 87_W | 112_G | 1.126 | 0.51 |
| 239_E | 355_K | 1.126 | 0.51 |
| 15_S | 19_L | 1.126 | 0.51 |
| 181_V | 306_L | 1.126 | 0.51 |
| 294_F | 300_V | 1.122 | 0.51 |
| 183_A | 262_L | 1.122 | 0.51 |
| 102_I | 341_I | 1.120 | 0.51 |
| 99_V | 136_S | 1.118 | 0.51 |
| 77_W | 87_W | 1.117 | 0.50 |
| 92_D | 355_K | 1.116 | 0.50 |
| 61_L | 130_M | 1.114 | 0.50 |
| 69_D | 77_W | 1.111 | 0.50 |
| 215_F | 328_C | 1.111 | 0.50 |
| 77_W | 86_W | 1.106 | 0.49 |
| 85_Y | 177_F | 1.105 | 0.49 |
| 156_W | 178_P | 1.100 | 0.49 |
| 95_S | 355_K | 1.096 | 0.48 |
| 138_F | 353_H | 1.096 | 0.48 |
| 83_R | 114_G | 1.095 | 0.48 |
| 51_F | 313_K | 1.090 | 0.48 |
| 93_Y | 140_G | 1.090 | 0.48 |
| 37_A | 42_A | 1.085 | 0.47 |
| 76_K | 270_G | 1.084 | 0.47 |
| 326_S | 329_K | 1.082 | 0.47 |
| 149_R | 190_A | 1.082 | 0.47 |
| 95_S | 239_E | 1.082 | 0.47 |
| 92_D | 352_P | 1.081 | 0.47 |
| 23_Y | 120_A | 1.080 | 0.47 |
| 71_L | 164_L | 1.078 | 0.47 |
| 95_S | 350_G | 1.077 | 0.47 |
| 138_F | 355_K | 1.076 | 0.46 |
| 95_S | 98_S | 1.076 | 0.46 |
| 169_S | 263_K | 1.075 | 0.46 |
| 244_W | 252_H | 1.073 | 0.46 |
| 80_F | 236_W | 1.071 | 0.46 |
| 64_V | 212_I | 1.070 | 0.46 |
| 178_P | 342_P | 1.070 | 0.46 |
| 188_G | 268_L | 1.069 | 0.46 |
| 170_R | 181_V | 1.067 | 0.46 |
| 247_Q | 250_W | 1.065 | 0.45 |
| 97_T | 354_K | 1.063 | 0.45 |
| 46_L | 126_V | 1.063 | 0.45 |
| 114_G | 184_G | 1.063 | 0.45 |
| 274_A | 296_L | 1.063 | 0.45 |
| 119_H | 270_G | 1.062 | 0.45 |
| 84_P | 116_P | 1.061 | 0.45 |
| 81_G | 115_S | 1.061 | 0.45 |
| 92_D | 96_N | 1.058 | 0.45 |
| 200_S | 305_L | 1.056 | 0.44 |
| 349_L | 354_K | 1.055 | 0.44 |
| 120_A | 184_G | 1.055 | 0.44 |
| 283_S | 346_A | 1.054 | 0.44 |
| 45_V | 57_V | 1.053 | 0.44 |
| 34_S | 85_Y | 1.051 | 0.44 |
| 95_S | 352_P | 1.051 | 0.44 |
| 69_D | 118_G | 1.049 | 0.44 |
| 134_T | 303_L | 1.049 | 0.44 |
| 174_A | 252_H | 1.047 | 0.44 |
| 123_T | 176_H | 1.046 | 0.44 |
| 170_R | 175_A | 1.045 | 0.43 |
| 120_A | 298_S | 1.044 | 0.43 |
| 110_E | 114_G | 1.044 | 0.43 |
| 66_V | 184_G | 1.044 | 0.43 |
| 170_R | 342_P | 1.041 | 0.43 |
| 99_V | 350_G | 1.039 | 0.43 |
| 75_F | 206_L | 1.038 | 0.43 |
| 344_C | 348_V | 1.037 | 0.43 |
| 120_A | 167_C | 1.035 | 0.42 |
| 99_V | 353_H | 1.035 | 0.42 |
| 92_D | 139_Q | 1.034 | 0.42 |
| 30_F | 49_I | 1.034 | 0.42 |
| 350_G | 354_K | 1.034 | 0.42 |
| 349_L | 353_H | 1.033 | 0.42 |
| 12_G | 16_T | 1.033 | 0.42 |
| 167_C | 181_V | 1.030 | 0.42 |
| 75_F | 79_L | 1.030 | 0.42 |
| 92_D | 350_G | 1.028 | 0.42 |
| 6_N | 10_D | 1.024 | 0.41 |
| 175_A | 267_T | 1.023 | 0.41 |
| 349_L | 352_P | 1.021 | 0.41 |
| 198_I | 220_A | 1.021 | 0.41 |
| 47_F | 105_F | 1.021 | 0.41 |
| 72_N | 177_F | 1.020 | 0.41 |
| 29_W | 33_V | 1.018 | 0.41 |
| 54_Q | 274_A | 1.018 | 0.41 |
| 178_P | 323_Y | 1.017 | 0.41 |
| 58_G | 188_G | 1.017 | 0.41 |
| 187_S | 222_G | 1.017 | 0.41 |
| 102_I | 134_T | 1.016 | 0.41 |
| 52_H | 302_S | 1.015 | 0.41 |
| 225_L | 296_L | 1.013 | 0.40 |
| 224_Y | 228_K | 1.013 | 0.40 |
| 62_L | 66_V | 1.013 | 0.40 |
| 239_E | 247_Q | 1.010 | 0.40 |
| 181_V | 339_S | 1.009 | 0.40 |
| 53_L | 78_I | 1.009 | 0.40 |
| 72_N | 121_M | 1.008 | 0.40 |
| 29_W | 54_Q | 1.006 | 0.40 |
| 70_W | 104_Q | 1.004 | 0.40 |
| 184_G | 188_G | 1.004 | 0.40 |
| 139_Q | 350_G | 1.001 | 0.39 |
| 68_G | 75_F | 1.001 | 0.39 |
| 95_S | 353_H | 1.001 | 0.39 |
| 207_K | 273_L | 1.000 | 0.39 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- See coupling matrix (for protein design or mutagenesis)
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4px7A | 1 | 0.577 | 99.8 | 0.748 | Contact Map |
| 1d2tA | 1 | 0.5238 | 99.8 | 0.765 | Contact Map |
| 4citA | 1 | 0.5322 | 99.7 | 0.775 | Contact Map |
| 1qi9A | 2 | 0.6639 | 99.7 | 0.779 | Contact Map |
| 2ipbA | 4 | 0.5378 | 99.7 | 0.786 | Contact Map |
| 1up8A | 4 | 0.591 | 99.7 | 0.788 | Contact Map |
| 3w36A | 2 | 0.5686 | 99.6 | 0.793 | Contact Map |
| 3bb0A | 1 | 0.6527 | 99.4 | 0.825 | Contact Map |
| 4b19A | 1 | 0.084 | 4.4 | 0.973 | Contact Map |
| 2z0vA | 2 | 0.1989 | 2.1 | 0.977 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)