| ETV145 ED multiole |
| ID: | 1444199851 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 105 (93) | ||
| Sequences: | 369 (198) | ||
| Seq/Len: | 3.968 | ||
| Nf(neff/√len): | 20.5 |
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.968).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 40_E | 43_R | 2.198 | 1.00 |
| 73_Q | 83_K | 1.895 | 1.00 |
| 42_A | 46_G | 1.859 | 1.00 |
| 11_L | 24_I | 1.714 | 0.99 |
| 6_Q | 38_P | 1.673 | 0.99 |
| 38_P | 80_Y | 1.566 | 0.98 |
| 24_I | 41_V | 1.499 | 0.98 |
| 54_M | 61_R | 1.495 | 0.97 |
| 66_Y | 71_I | 1.446 | 0.97 |
| 26_W | 33_F | 1.434 | 0.97 |
| 61_R | 64_R | 1.433 | 0.96 |
| 28_G | 32_E | 1.429 | 0.96 |
| 25_A | 36_I | 1.374 | 0.95 |
| 12_V | 31_M | 1.368 | 0.95 |
| 79_R | 82_Y | 1.350 | 0.95 |
| 54_M | 64_R | 1.332 | 0.94 |
| 57_D | 69_K | 1.290 | 0.93 |
| 13_A | 48_Q | 1.245 | 0.91 |
| 47_I | 76_A | 1.242 | 0.91 |
| 49_K | 59_L | 1.234 | 0.90 |
| 48_Q | 58_K | 1.233 | 0.90 |
| 58_K | 62_S | 1.218 | 0.90 |
| 10_F | 15_L | 1.216 | 0.90 |
| 58_K | 61_R | 1.210 | 0.89 |
| 35_L | 41_V | 1.205 | 0.89 |
| 41_V | 65_Y | 1.175 | 0.87 |
| 57_D | 67_Y | 1.165 | 0.87 |
| 66_Y | 74_K | 1.159 | 0.86 |
| 78_E | 88_P | 1.142 | 0.85 |
| 6_Q | 79_R | 1.142 | 0.85 |
| 81_V | 85_V | 1.141 | 0.85 |
| 27_T | 32_E | 1.140 | 0.85 |
| 29_R | 75_V | 1.134 | 0.85 |
| 42_A | 61_R | 1.130 | 0.84 |
| 59_L | 66_Y | 1.124 | 0.84 |
| 45_W | 49_K | 1.110 | 0.83 |
| 5_L | 43_R | 1.105 | 0.83 |
| 67_Y | 71_I | 1.100 | 0.82 |
| 18_P | 22_H | 1.097 | 0.82 |
| 73_Q | 78_E | 1.097 | 0.82 |
| 72_M | 81_V | 1.096 | 0.82 |
| 61_R | 66_Y | 1.084 | 0.81 |
| 65_Y | 77_G | 1.081 | 0.81 |
| 51_R | 63_L | 1.074 | 0.80 |
| 56_Y | 61_R | 1.074 | 0.80 |
| 32_E | 80_Y | 1.067 | 0.79 |
| 77_G | 83_K | 1.065 | 0.79 |
| 54_M | 58_K | 1.064 | 0.79 |
| 34_K | 81_V | 1.057 | 0.79 |
| 75_V | 84_F | 1.056 | 0.78 |
| 46_G | 54_M | 1.041 | 0.77 |
| 34_K | 78_E | 1.040 | 0.77 |
| 45_W | 54_M | 1.035 | 0.76 |
| 75_V | 83_K | 1.024 | 0.75 |
| 46_G | 61_R | 1.015 | 0.74 |
| 67_Y | 82_Y | 1.014 | 0.74 |
| 42_A | 60_S | 1.006 | 0.74 |
| 9_Q | 35_L | 1.002 | 0.73 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4co8A | 1 | 0.9333 | 100 | 0.02 | Contact Map |
| 3wtsC | 2 | 0.9905 | 100 | 0.121 | Contact Map |
| 1gvjA | 2 | 0.9905 | 100 | 0.123 | Contact Map |
| 1awcA | 1 | 0.9619 | 100 | 0.133 | Contact Map |
| 1bc8C | 1 | 0.8857 | 100 | 0.148 | Contact Map |
| 1hbxG | 2 | 0.8762 | 100 | 0.151 | Contact Map |
| 2daoA | 1 | 1 | 100 | 0.154 | Contact Map |
| 2lf8A | 1 | 1 | 100 | 0.156 | Contact Map |
| 4irgA | 1 | 0.8952 | 100 | 0.166 | Contact Map |
| 4irhA | 1 | 0.9048 | 100 | 0.167 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)