| A2AR_LABOS_1e_20 |
| ID: | 1427837529 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 310 (274) | ||
| Sequences: | 22152 (10155.8) | ||
| Seq/Len: | 80.847 | ||
| Nf(neff/√len): | 613.5 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 80.847).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 46_T | 82_A | 4.471 | 1.00 |
| 73_S | 156_W | 3.712 | 1.00 |
| 203_L | 207_R | 3.126 | 1.00 |
| 125_A | 155_V | 2.699 | 1.00 |
| 146_K | 150_C | 2.598 | 1.00 |
| 73_S | 122_H | 2.450 | 1.00 |
| 102_K | 106_G | 2.352 | 1.00 |
| 103_V | 107_I | 2.346 | 1.00 |
| 125_A | 151_I | 2.193 | 1.00 |
| 57_V | 72_V | 2.187 | 1.00 |
| 164_S | 168_L | 2.162 | 1.00 |
| 114_L | 160_A | 2.090 | 1.00 |
| 207_R | 210_Q | 2.027 | 1.00 |
| 60_S | 297_A | 2.011 | 1.00 |
| 80_L | 115_F | 1.929 | 1.00 |
| 131_Y | 136_Q | 1.924 | 1.00 |
| 77_A | 118_S | 1.871 | 1.00 |
| 88_F | 92_N | 1.865 | 1.00 |
| 110_A | 114_L | 1.837 | 1.00 |
| 260_C | 263_Y | 1.836 | 1.00 |
| 206_I | 210_Q | 1.832 | 1.00 |
| 239_V | 243_F | 1.807 | 1.00 |
| 252_F | 256_L | 1.805 | 1.00 |
| 33_A | 37_A | 1.796 | 1.00 |
| 54_V | 72_V | 1.778 | 1.00 |
| 131_Y | 135_T | 1.759 | 1.00 |
| 72_V | 76_T | 1.756 | 1.00 |
| 205_Y | 208_I | 1.755 | 1.00 |
| 98_W | 101_G | 1.747 | 1.00 |
| 58_L | 72_V | 1.722 | 1.00 |
| 46_T | 281_S | 1.693 | 1.00 |
| 63_L | 68_N | 1.688 | 1.00 |
| 247_W | 276_G | 1.666 | 1.00 |
| 74_L | 78_D | 1.662 | 1.00 |
| 149_K | 153_V | 1.626 | 1.00 |
| 101_G | 104_W | 1.595 | 1.00 |
| 122_H | 152_I | 1.594 | 1.00 |
| 51_I | 55_I | 1.592 | 1.00 |
| 43_I | 83_T | 1.582 | 1.00 |
| 242_V | 286_I | 1.581 | 1.00 |
| 151_I | 155_V | 1.578 | 1.00 |
| 132_W | 142_K | 1.577 | 1.00 |
| 144_T | 147_R | 1.545 | 1.00 |
| 117_T | 193_S | 1.544 | 1.00 |
| 129_D | 143_R | 1.542 | 1.00 |
| 105_C | 177_C | 1.525 | 1.00 |
| 283_N | 287_Y | 1.519 | 1.00 |
| 77_A | 119_S | 1.517 | 1.00 |
| 150_C | 154_I | 1.515 | 1.00 |
| 73_S | 152_I | 1.511 | 1.00 |
| 118_S | 159_S | 1.496 | 1.00 |
| 69_L | 152_I | 1.491 | 1.00 |
| 297_A | 300_K | 1.491 | 1.00 |
| 200_I | 204_V | 1.459 | 1.00 |
| 51_I | 79_I | 1.430 | 1.00 |
| 120_I | 197_P | 1.429 | 1.00 |
| 235_V | 290_F | 1.413 | 1.00 |
| 88_F | 104_W | 1.407 | 0.99 |
| 131_Y | 207_R | 1.401 | 0.99 |
| 65_A | 68_N | 1.371 | 0.99 |
| 207_R | 211_V | 1.368 | 0.99 |
| 103_V | 106_G | 1.364 | 0.99 |
| 53_V | 288_T | 1.361 | 0.99 |
| 76_T | 80_L | 1.360 | 0.99 |
| 210_Q | 214_T | 1.342 | 0.99 |
| 47_V | 79_I | 1.338 | 0.99 |
| 202_I | 206_I | 1.300 | 0.99 |
| 120_I | 124_C | 1.296 | 0.99 |
| 110_A | 167_L | 1.291 | 0.99 |
| 57_V | 64_K | 1.270 | 0.99 |
| 166_P | 169_S | 1.262 | 0.99 |
| 110_A | 160_A | 1.259 | 0.99 |
| 238_V | 243_F | 1.255 | 0.99 |
| 129_D | 133_S | 1.250 | 0.99 |
| 52_L | 56_A | 1.249 | 0.99 |
| 184_W | 188_S | 1.242 | 0.98 |
| 278_C | 282_L | 1.237 | 0.98 |
| 190_S | 195_F | 1.237 | 0.98 |
| 139_Y | 143_R | 1.230 | 0.98 |
| 248_F | 252_F | 1.229 | 0.98 |
| 237_A | 241_G | 1.226 | 0.98 |
| 250_F | 253_S | 1.225 | 0.98 |
| 201_M | 205_Y | 1.196 | 0.98 |
| 266_P | 269_L | 1.187 | 0.98 |
| 133_S | 137_A | 1.177 | 0.98 |
| 55_I | 59_T | 1.176 | 0.98 |
| 215_R | 219_I | 1.164 | 0.97 |
| 135_T | 211_V | 1.159 | 0.97 |
| 121_V | 155_V | 1.154 | 0.97 |
| 123_L | 201_M | 1.145 | 0.97 |
| 209_Y | 213_K | 1.143 | 0.97 |
| 124_C | 200_I | 1.140 | 0.97 |
| 49_G | 75_A | 1.139 | 0.97 |
| 43_I | 90_L | 1.138 | 0.97 |
| 60_S | 63_L | 1.137 | 0.97 |
| 113_V | 163_S | 1.129 | 0.97 |
| 239_V | 242_V | 1.128 | 0.97 |
| 173_I | 271_K | 1.126 | 0.97 |
| 54_V | 57_V | 1.118 | 0.96 |
| 131_Y | 203_L | 1.110 | 0.96 |
| 60_S | 300_K | 1.110 | 0.96 |
| 121_V | 158_I | 1.103 | 0.96 |
| 186_I | 189_S | 1.102 | 0.96 |
| 57_V | 63_L | 1.100 | 0.96 |
| 229_E | 233_T | 1.091 | 0.96 |
| 40_S | 90_L | 1.089 | 0.96 |
| 133_S | 138_V | 1.088 | 0.96 |
| 136_Q | 142_K | 1.086 | 0.96 |
| 198_C | 244_V | 1.083 | 0.96 |
| 268_T | 272_F | 1.075 | 0.95 |
| 159_S | 163_S | 1.071 | 0.95 |
| 263_Y | 267_D | 1.067 | 0.95 |
| 240_M | 243_F | 1.065 | 0.95 |
| 70_F | 148_V | 1.057 | 0.95 |
| 124_C | 197_P | 1.053 | 0.95 |
| 82_A | 86_M | 1.052 | 0.95 |
| 278_C | 281_S | 1.051 | 0.94 |
| 56_A | 294_F | 1.049 | 0.94 |
| 238_V | 290_F | 1.048 | 0.94 |
| 194_F | 248_F | 1.044 | 0.94 |
| 114_L | 163_S | 1.044 | 0.94 |
| 49_G | 281_S | 1.042 | 0.94 |
| 146_K | 149_K | 1.041 | 0.94 |
| 53_V | 57_V | 1.040 | 0.94 |
| 47_V | 82_A | 1.039 | 0.94 |
| 150_C | 153_V | 1.039 | 0.94 |
| 111_L | 115_F | 1.033 | 0.94 |
| 181_D | 184_W | 1.031 | 0.94 |
| 48_V | 51_I | 1.030 | 0.94 |
| 73_S | 149_K | 1.024 | 0.93 |
| 147_R | 150_C | 1.021 | 0.93 |
| 244_V | 248_F | 1.021 | 0.93 |
| 54_V | 76_T | 1.021 | 0.93 |
| 45_F | 281_S | 1.018 | 0.93 |
| 194_F | 251_F | 1.011 | 0.93 |
| 126_I | 236_L | 1.008 | 0.93 |
| 232_F | 235_V | 1.007 | 0.93 |
| 242_V | 282_L | 1.004 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2rh1A | 1 | 0.8903 | 100 | 0.275 | Contact Map |
| 3uonA | 1 | 0.8871 | 100 | 0.304 | Contact Map |
| 3pblA | 2 | 0.8677 | 100 | 0.304 | Contact Map |
| 4pxzA | 1 | 0.8613 | 100 | 0.312 | Contact Map |
| 2z73A | 2 | 0.9774 | 100 | 0.314 | Contact Map |
| 4u15A | 2 | 0.8871 | 100 | 0.321 | Contact Map |
| 3oduA | 2 | 0.929 | 100 | 0.322 | Contact Map |
| 4grvA | 1 | 0.8839 | 100 | 0.324 | Contact Map |
| 3rzeA | 1 | 0.8742 | 100 | 0.325 | Contact Map |
| 3vw7A | 1 | 0.8677 | 100 | 0.326 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)