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OPENSEQ.org

Env

ID: 1413869124 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 856 (843)
Sequences: 4806 (370.9)
Seq/Len: 5.701
Nf(neff/√len): 12.8

HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 5.701).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
283_T453_L4.4981.00
632_D636_N3.8401.00
502_K607_A3.5351.00
747_R758_D3.4771.00
330_H334_S3.1581.00
658_Q662_E3.0201.00
825_G829_V2.8331.00
275_V278_T2.6681.00
755_L758_D2.5321.00
620_E624_N2.4461.00
567_Q629_M2.3751.00
97_K275_V2.3611.00
348_K351_E2.2011.00
332_N336_A2.1901.00
65_V208_V2.1061.00
746_I758_D2.0941.00
159_F174_A2.0771.00
230_N240_T2.0641.00
85_V229_N2.0601.00
659_E662_E2.0371.00
360_I467_I2.0341.00
722_P726_G2.0111.00
232_T268_E1.9911.00
641_L644_S1.9831.00
815_L818_T1.9751.00
167_G192_K1.9691.00
797_W800_L1.9411.00
817_A821_A1.9331.00
700_A758_D1.9221.00
630_E633_R1.9131.00
17_W20_M1.9111.00
354_G357_K1.8761.00
25_L31_T1.8751.00
12_R20_M1.8581.00
232_T236_T1.8541.00
849_Q852_E1.8271.00
11_W21_L1.8131.00
211_E379_G1.7751.00
293_E337_K1.7661.00
308_R315_R1.7591.00
46_K49_T1.7551.00
790_W794_K1.7211.00
381_E385_C1.7161.00
655_K658_Q1.7151.00
360_I465_S1.7121.00
848_R852_E1.6901.00
21_L25_L1.6841.00
231_K267_E1.6811.00
816_N824_E1.6731.00
21_L26_M1.6651.00
114_Q202_T1.6591.00
726_G736_E1.6471.00
277_F352_Q1.6461.00
849_Q855_L1.6381.00
496_V646_I1.6290.99
270_V287_Q1.6270.99
92_N630_E1.6170.99
46_K636_N1.6140.99
850_G855_L1.6010.99
564_H567_Q1.5960.99
269_E348_K1.5950.99
223_F655_K1.5950.99
290_T344_Q1.5930.99
852_E855_L1.5880.99
846_R849_Q1.5800.99
92_N238_P1.5770.99
721_L756_I1.5660.99
378_C385_C1.5620.99
270_V277_F1.5580.99
198_T202_T1.5560.99
287_Q291_S1.5530.99
846_R852_E1.5520.99
11_W26_M1.5490.99
182_I192_K1.5480.99
328_Q334_S1.5380.99
837_C840_I1.5370.99
740_R796_W1.5370.99
325_N419_R1.5360.99
22_L30_A1.5250.99
824_E829_V1.5220.99
756_I775_L1.5210.99
275_V279_D1.5140.99
833_V837_C1.5100.99
49_T99_D1.5070.99
836_A839_A1.5020.99
47_E629_M1.5000.99
23_G30_A1.4990.99
425_N432_K1.4860.99
471_G476_R1.4830.99
369_P373_T1.4820.99
378_C381_E1.4800.99
12_R23_G1.4790.99
12_R22_L1.4790.99
223_F287_Q1.4650.99
844_P848_R1.4610.99
12_R30_A1.4550.99
5_E21_L1.4520.99
20_M26_M1.4350.98
731_E736_E1.4350.98
705_V732_G1.4340.98
588_K646_I1.4320.98
848_R855_L1.4280.98
219_A225_I1.4280.98
62_D65_V1.4280.98
847_I855_L1.4260.98
92_N633_R1.4240.98
178_K195_S1.4240.98
22_L29_S1.4240.98
230_N236_T1.4200.98
847_I850_G1.4110.98
747_R755_L1.4090.98
844_P849_Q1.4060.98
293_E297_T1.4050.98
20_M25_L1.4010.98
783_E786_G1.4000.98
837_C841_R1.3960.98
9_H12_R1.3890.98
813_S817_A1.3870.98
367_G370_E1.3770.98
330_H415_T1.3740.98
725_R743_D1.3690.98
770_R773_D1.3620.98
46_K632_D1.3610.98
232_T240_T1.3590.97
380_G385_C1.3530.97
619_L630_E1.3520.97
9_H23_G1.3500.97
378_C384_Y1.3490.97
515_I519_F1.3480.97
846_R855_L1.3470.97
7_Y26_M1.3470.97
305_K319_T1.3410.97
4_K7_Y1.3400.97
518_L662_E1.3400.97
7_Y12_R1.3380.97
4_K26_M1.3360.97
723_T726_G1.3350.97
24_M32_E1.3320.97
847_I852_E1.3310.97
788_R801_Q1.3260.97
722_P824_E1.3220.97
164_S195_S1.3190.97
435_Y445_C1.3190.97
121_K429_K1.3140.97
845_R851_L1.3120.97
213_I216_H1.3090.97
756_I774_L1.3070.97
427_W445_C1.3070.97
382_F385_C1.3060.97
223_F658_Q1.3010.96
792_A801_Q1.2990.96
632_D635_I1.2890.96
667_A674_N1.2830.96
270_V346_A1.2830.96
378_C383_F1.2810.96
843_I847_I1.2760.96
429_K432_K1.2750.96
21_L24_M1.2750.96
725_R741_D1.2740.96
361_F366_G1.2700.96
651_N658_Q1.2660.96
724_P727_P1.2640.96
381_E384_Y1.2620.96
11_W24_M1.2620.96
846_R850_G1.2600.96
195_S200_V1.2580.96
422_Q430_V1.2570.95
288_L348_K1.2570.95
844_P850_G1.2560.95
49_T659_E1.2550.95
844_P852_E1.2550.95
386_N390_L1.2520.95
269_E289_N1.2510.95
377_N384_Y1.2480.95
368_D376_F1.2470.95
9_H30_A1.2440.95
565_L646_I1.2440.95
690_G693_V1.2430.95
844_P855_L1.2420.95
165_I192_K1.2420.95
427_W438_P1.2390.95
845_R852_E1.2390.95
370_E376_F1.2370.95
368_D374_H1.2350.95
825_G833_V1.2340.95
740_R746_I1.2310.95
662_E665_K1.2310.95
592_L602_L1.2290.95
211_E223_F1.2290.95
308_R316_A1.2280.95
801_Q824_E1.2240.95
421_K424_I1.2220.95
309_I317_F1.2220.95
289_N344_Q1.2210.94
380_G383_F1.2200.94
309_I315_R1.2170.94
162_S168_K1.2160.94
168_K180_D1.2150.94
5_E11_W1.2140.94
114_Q198_T1.2140.94
432_K567_Q1.2110.94
379_G444_R1.2100.94
368_D371_I1.2080.94
801_Q825_G1.2080.94
20_M24_M1.2060.94
849_Q853_R1.2050.94
330_H336_A1.2040.94
725_R731_E1.2030.94
333_I363_Q1.2010.94
781_I801_Q1.1980.94
23_G27_I1.1970.94
677_N683_K1.1950.94
160_N163_T1.1900.93
496_V565_L1.1900.93
665_K677_N1.1890.93
223_F490_K1.1870.93
3_V671_N1.1850.93
458_G466_E1.1850.93
700_A747_R1.1820.93
427_W435_Y1.1730.93
788_R792_A1.1690.93
24_M31_T1.1690.93
721_L732_G1.1660.93
805_Q809_N1.1650.92
844_P847_I1.1640.92
490_K646_I1.1610.92
25_L731_E1.1610.92
370_E374_H1.1610.92
202_T425_N1.1590.92
210_F215_I1.1590.92
811_A814_L1.1570.92
328_Q332_N1.1570.92
65_V379_G1.1560.92
383_F386_N1.1490.92
300_N442_Q1.1480.92
721_L736_E1.1450.92
20_M31_T1.1440.92
828_R831_E1.1440.92
840_I843_I1.1400.91
851_L856_L1.1400.91
333_I348_K1.1390.91
380_G384_Y1.1390.91
377_N381_E1.1380.91
420_I437_P1.1360.91
436_A439_I1.1350.91
21_L801_Q1.1350.91
157_C162_S1.1350.91
629_M632_D1.1290.91
816_N821_A1.1260.91
775_L800_L1.1260.91
369_P372_V1.1250.91
353_F356_N1.1250.91
174_A632_D1.1240.91
9_H20_M1.1230.91
438_P445_C1.1220.91
821_A833_V1.1200.90
784_L792_A1.1200.90
845_R849_Q1.1190.90
290_T337_K1.1180.90
557_R567_Q1.1170.90
378_C382_F1.1170.90
788_R825_G1.1160.90
7_Y11_W1.1150.90
761_R769_H1.1150.90
818_T842_H1.1140.90
377_N382_F1.1120.90
369_P432_K1.1100.90
437_P445_C1.1090.90
671_N683_K1.1090.90
792_A829_V1.1070.90
515_I518_L1.1050.90
845_R848_R1.1050.90
816_N825_G1.1040.90
295_N446_S1.1040.90
818_T824_E1.1030.90
349_L468_F1.1020.89
500_K553_N1.1020.89
676_T683_K1.1000.89
720_H758_D1.1000.89
165_I200_V1.0990.89
843_I846_R1.0980.89
727_P743_D1.0950.89
164_S200_V1.0950.89
371_I375_S1.0950.89
636_N644_S1.0920.89
63_T774_L1.0890.89
21_L796_W1.0870.89
698_V705_V1.0870.89
361_F368_D1.0860.89
790_W793_L1.0860.89
189_T369_P1.0860.89
106_E633_R1.0850.88
789_G794_K1.0840.88
162_S180_D1.0840.88
745_S749_V1.0810.88
784_L800_L1.0810.88
758_D764_C1.0810.88
722_P825_G1.0810.88
781_I792_A1.0800.88
232_T269_E1.0780.88
196_C199_S1.0780.88
161_I172_E1.0780.88
456_R459_G1.0770.88
361_F370_E1.0760.88
369_P425_N1.0750.88
383_F391_F1.0750.88
630_E634_E1.0720.88
750_N756_I1.0720.88
781_I804_S1.0690.87
365_S374_H1.0690.87
809_N836_A1.0670.87
849_Q856_L1.0670.87
162_S167_G1.0670.87
732_G788_R1.0660.87
723_T756_I1.0650.87
805_Q818_T1.0640.87
21_L432_K1.0630.87
371_I376_F1.0610.87
428_Q433_A1.0600.87
229_N232_T1.0560.87
102_E851_L1.0550.87
348_K429_K1.0550.87
106_E172_E1.0540.86
5_E796_W1.0530.86
202_T619_L1.0520.86
420_I438_P1.0510.86
723_T736_E1.0500.86
806_E814_L1.0500.86
5_E9_H1.0490.86
619_L646_I1.0490.86
851_L855_L1.0470.86
334_S446_S1.0470.86
843_I848_R1.0470.86
843_I850_G1.0460.86
801_Q804_S1.0460.86
633_R731_E1.0450.86
383_F390_L1.0450.86
92_N270_V1.0450.86
827_D830_I1.0450.86
346_A395_W1.0440.86
849_Q854_I1.0430.86
306_R322_K1.0420.86
721_L750_N1.0420.86
377_N385_C1.0420.86
25_L801_Q1.0420.86
218_C222_G1.0400.85
21_L721_L1.0400.85
387_S390_L1.0390.85
756_I800_L1.0390.85
159_F162_S1.0380.85
46_K62_D1.0370.85
796_W812_V1.0350.85
723_T740_R1.0320.85
834_Q838_R1.0320.85
371_I374_H1.0320.85
331_C338_W1.0320.85
830_I833_V1.0310.85
9_H19_T1.0310.85
230_N388_T1.0270.84
754_A758_D1.0270.84
441_G445_C1.0270.84
674_N677_N1.0260.84
172_E200_V1.0240.84
168_K175_F1.0240.84
784_L787_R1.0240.84
293_E446_S1.0240.84
378_C386_N1.0230.84
85_V630_E1.0220.84
164_S170_Q1.0220.84
802_Y806_E1.0210.84
427_W437_P1.0210.84
721_L816_N1.0210.84
847_I854_I1.0200.84
583_V698_V1.0200.84
632_D659_E1.0200.84
629_M788_R1.0190.84
721_L774_L1.0180.84
418_C445_C1.0170.84
377_N380_G1.0170.84
4_K29_S1.0160.84
236_T633_R1.0160.84
847_I856_L1.0150.84
586_Y589_D1.0150.84
723_T824_E1.0140.83
387_S391_F1.0140.83
325_N334_S1.0130.83
385_C390_L1.0110.83
12_R25_L1.0100.83
203_Q207_K1.0100.83
385_C391_F1.0100.83
565_L580_I1.0090.83
361_F371_I1.0080.83
574_K577_Q1.0080.83
848_R853_R1.0080.83
838_R844_P1.0080.83
12_R31_T1.0080.83
431_G445_C1.0070.83
7_Y20_M1.0070.83
207_K213_I1.0070.83
746_I750_N1.0060.83
333_I341_T1.0050.83
426_M433_A1.0050.83
92_N174_A1.0040.83
496_V632_D1.0030.83
422_Q431_G1.0030.83
756_I804_S1.0020.82
722_P736_E1.0010.82
7_Y21_L1.0000.82
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Sep14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4ncoA30.48831000.448Contact Map
4jzzA10.39251000.561Contact Map
3lqaG10.33531000.634Contact Map
2bf1A10.34811000.639Contact Map
4i3sG10.21031000.824Contact Map
1qbzA30.14021000.863Contact Map
3tygA10.17061000.867Contact Map
4jpkA10.17761000.889Contact Map
1envA10.1321000.893Contact Map
3p30A10.09811000.899Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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