| bbb |
| ID: | 1402456095 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 86 (84) | ||
| Sequences: | 897 (613.4) | ||
| Seq/Len: | 10.679 | ||
| Nf(neff/√len): | 66.9 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 10.679).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 48_D | 51_G | 3.504 | 1.00 |
| 59_N | 62_K | 3.456 | 1.00 |
| 43_R | 56_D | 3.391 | 1.00 |
| 38_D | 63_G | 3.294 | 1.00 |
| 2_Q | 70_M | 2.800 | 1.00 |
| 64_T | 72_A | 2.377 | 1.00 |
| 27_I | 77_V | 2.187 | 1.00 |
| 38_D | 62_K | 2.088 | 1.00 |
| 29_S | 61_L | 1.864 | 1.00 |
| 55_W | 59_N | 1.796 | 1.00 |
| 7_K | 11_E | 1.794 | 1.00 |
| 71_K | 75_D | 1.769 | 1.00 |
| 26_F | 76_K | 1.766 | 1.00 |
| 33_Q | 37_G | 1.703 | 1.00 |
| 41_T | 55_W | 1.677 | 1.00 |
| 68_D | 72_A | 1.576 | 1.00 |
| 20_A | 23_E | 1.552 | 1.00 |
| 10_E | 13_K | 1.509 | 1.00 |
| 26_F | 30_H | 1.426 | 1.00 |
| 46_M | 52_K | 1.371 | 0.99 |
| 66_K | 70_M | 1.356 | 0.99 |
| 78_E | 81_K | 1.298 | 0.99 |
| 32_K | 73_Y | 1.268 | 0.99 |
| 31_Y | 73_Y | 1.216 | 0.98 |
| 15_L | 19_P | 1.205 | 0.98 |
| 68_D | 71_K | 1.193 | 0.98 |
| 6_D | 10_E | 1.191 | 0.98 |
| 39_I | 55_W | 1.184 | 0.98 |
| 45_G | 51_G | 1.180 | 0.98 |
| 23_E | 76_K | 1.174 | 0.98 |
| 21_D | 24_M | 1.171 | 0.98 |
| 36_V | 40_N | 1.149 | 0.97 |
| 61_L | 64_T | 1.147 | 0.97 |
| 34_A | 70_M | 1.113 | 0.96 |
| 67_E | 70_M | 1.081 | 0.95 |
| 23_E | 84_Y | 1.034 | 0.94 |
| 11_E | 81_K | 1.030 | 0.94 |
| 19_P | 24_M | 1.029 | 0.94 |
| 4_E | 8_A | 1.027 | 0.94 |
| 3_A | 7_K | 1.022 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2cb8A | 2 | 1 | 100 | 0.025 | Contact Map |
| 2lbbA | 1 | 1 | 100 | 0.026 | Contact Map |
| 3flvA | 2 | 1 | 100 | 0.029 | Contact Map |
| 3epyA | 2 | 1 | 100 | 0.033 | Contact Map |
| 3fp5A | 1 | 1 | 100 | 0.043 | Contact Map |
| 1st7A | 1 | 1 | 100 | 0.043 | Contact Map |
| 2copA | 1 | 1 | 100 | 0.045 | Contact Map |
| 2cquA | 1 | 1 | 100 | 0.047 | Contact Map |
| 1hbkA | 1 | 1 | 100 | 0.05 | Contact Map |
| 2wh5A | 6 | 0.9884 | 100 | 0.068 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)