| Hsp70_Arch |
| ID: | 1402403117 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 613 (606) | ||
| Sequences: | 5426 (2879.3) | ||
| Seq/Len: | 8.954 | ||
| Nf(neff/√len): | 117.0 |
Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 8.954).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 200_D | 291_R | 5.640 | 1.00 |
| 117_E | 144_D | 5.137 | 1.00 |
| 211_A | 287_P | 4.770 | 1.00 |
| 212_I | 276_L | 3.993 | 1.00 |
| 72_A | 206_T | 3.967 | 1.00 |
| 173_F | 292_V | 3.737 | 1.00 |
| 184_I | 299_A | 3.423 | 1.00 |
| 330_E | 336_K | 3.364 | 1.00 |
| 29_S | 40_F | 3.141 | 1.00 |
| 374_T | 386_P | 3.080 | 1.00 |
| 196_S | 299_A | 3.057 | 1.00 |
| 217_A | 229_R | 3.056 | 1.00 |
| 327_K | 331_D | 2.981 | 1.00 |
| 219_D | 280_K | 2.850 | 1.00 |
| 254_S | 275_T | 2.819 | 1.00 |
| 415_H | 429_S | 2.802 | 1.00 |
| 342_D | 345_E | 2.730 | 1.00 |
| 413_T | 434_N | 2.628 | 1.00 |
| 171_L | 182_V | 2.594 | 1.00 |
| 319_P | 322_I | 2.519 | 1.00 |
| 385_T | 402_F | 2.514 | 1.00 |
| 117_E | 146_K | 2.478 | 1.00 |
| 498_Y | 501_Q | 2.424 | 1.00 |
| 485_K | 489_E | 2.416 | 1.00 |
| 386_P | 389_P | 2.394 | 1.00 |
| 523_E | 549_R | 2.377 | 1.00 |
| 104_K | 108_E | 2.346 | 1.00 |
| 4_I | 146_K | 2.345 | 1.00 |
| 175_L | 208_M | 2.296 | 1.00 |
| 216_I | 274_M | 2.288 | 1.00 |
| 285_I | 322_I | 2.269 | 1.00 |
| 281_L | 319_P | 2.259 | 1.00 |
| 326_R | 339_G | 2.241 | 1.00 |
| 417_V | 429_S | 2.224 | 1.00 |
| 561_K | 565_D | 2.213 | 1.00 |
| 37_G | 56_E | 2.174 | 1.00 |
| 5_I | 16_A | 2.144 | 1.00 |
| 280_K | 283_E | 2.143 | 1.00 |
| 496_E | 499_A | 2.125 | 1.00 |
| 208_M | 288_I | 2.124 | 1.00 |
| 216_I | 242_A | 2.113 | 1.00 |
| 465_V | 472_K | 2.093 | 1.00 |
| 490_R | 494_E | 2.091 | 1.00 |
| 539_E | 543_N | 2.088 | 1.00 |
| 293_K | 332_Y | 2.062 | 1.00 |
| 556_N | 559_N | 2.049 | 1.00 |
| 171_L | 184_I | 2.038 | 1.00 |
| 210_E | 214_N | 2.015 | 1.00 |
| 52_M | 98_F | 2.010 | 1.00 |
| 328_Y | 331_D | 1.996 | 1.00 |
| 281_L | 322_I | 1.995 | 1.00 |
| 84_K | 87_D | 1.981 | 1.00 |
| 515_A | 548_L | 1.978 | 1.00 |
| 219_D | 222_K | 1.974 | 1.00 |
| 376_S | 384_A | 1.960 | 1.00 |
| 105_K | 109_A | 1.955 | 1.00 |
| 289_V | 324_Y | 1.950 | 1.00 |
| 213_V | 239_R | 1.915 | 1.00 |
| 294_G | 298_K | 1.905 | 1.00 |
| 256_D | 273_K | 1.903 | 1.00 |
| 550_K | 553_E | 1.891 | 1.00 |
| 542_T | 546_K | 1.860 | 1.00 |
| 15_A | 26_V | 1.846 | 1.00 |
| 4_I | 355_G | 1.845 | 1.00 |
| 546_K | 550_K | 1.841 | 1.00 |
| 559_N | 563_L | 1.838 | 1.00 |
| 184_I | 310_I | 1.834 | 1.00 |
| 68_G | 86_F | 1.830 | 1.00 |
| 573_E | 576_A | 1.804 | 1.00 |
| 534_D | 537_T | 1.794 | 1.00 |
| 139_T | 485_K | 1.775 | 1.00 |
| 294_G | 297_D | 1.752 | 1.00 |
| 500_E | 504_K | 1.752 | 1.00 |
| 565_D | 569_K | 1.743 | 1.00 |
| 368_L | 393_T | 1.742 | 1.00 |
| 321_R | 344_M | 1.737 | 1.00 |
| 503_R | 507_E | 1.733 | 1.00 |
| 212_I | 242_A | 1.730 | 1.00 |
| 576_A | 580_Q | 1.730 | 1.00 |
| 530_G | 538_K | 1.716 | 1.00 |
| 286_S | 290_E | 1.715 | 1.00 |
| 132_Q | 136_D | 1.710 | 1.00 |
| 103_I | 106_D | 1.703 | 1.00 |
| 15_A | 39_A | 1.700 | 1.00 |
| 312_K | 338_E | 1.699 | 1.00 |
| 121_T | 351_A | 1.698 | 1.00 |
| 243_E | 247_I | 1.691 | 1.00 |
| 537_T | 577_K | 1.657 | 1.00 |
| 415_H | 432_M | 1.654 | 1.00 |
| 216_I | 238_L | 1.646 | 1.00 |
| 139_T | 144_D | 1.627 | 1.00 |
| 17_A | 352_A | 1.624 | 1.00 |
| 285_I | 289_V | 1.617 | 1.00 |
| 96_S | 134_T | 1.607 | 1.00 |
| 138_G | 148_I | 1.596 | 1.00 |
| 17_A | 348_A | 1.595 | 1.00 |
| 399_S | 449_E | 1.589 | 1.00 |
| 413_T | 432_M | 1.581 | 1.00 |
| 9_L | 134_T | 1.572 | 1.00 |
| 257_I | 276_L | 1.568 | 1.00 |
| 102_K | 106_D | 1.566 | 1.00 |
| 423_L | 509_I | 1.566 | 1.00 |
| 216_I | 257_I | 1.566 | 1.00 |
| 20_I | 25_T | 1.563 | 1.00 |
| 297_D | 332_Y | 1.551 | 1.00 |
| 90_F | 95_I | 1.548 | 1.00 |
| 84_K | 89_E | 1.543 | 1.00 |
| 280_K | 284_L | 1.540 | 1.00 |
| 293_K | 297_D | 1.538 | 1.00 |
| 399_S | 451_T | 1.532 | 1.00 |
| 489_E | 493_K | 1.518 | 1.00 |
| 73_A | 99_I | 1.515 | 1.00 |
| 224_E | 270_K | 1.500 | 1.00 |
| 282_E | 327_K | 1.498 | 1.00 |
| 46_F | 98_F | 1.496 | 1.00 |
| 245_A | 255_T | 1.492 | 1.00 |
| 493_K | 496_E | 1.487 | 1.00 |
| 38_K | 345_E | 1.486 | 1.00 |
| 175_L | 180_L | 1.478 | 1.00 |
| 93_Q | 136_D | 1.477 | 1.00 |
| 493_K | 497_Q | 1.477 | 1.00 |
| 218_D | 229_R | 1.476 | 1.00 |
| 241_A | 245_A | 1.472 | 1.00 |
| 494_E | 497_Q | 1.466 | 1.00 |
| 76_K | 79_T | 1.458 | 1.00 |
| 535_K | 539_E | 1.454 | 1.00 |
| 67_E | 232_R | 1.451 | 1.00 |
| 571_I | 575_G | 1.448 | 0.99 |
| 386_P | 422_P | 1.436 | 0.99 |
| 228_L | 262_I | 1.435 | 0.99 |
| 171_L | 296_I | 1.434 | 0.99 |
| 168_E | 312_K | 1.423 | 0.99 |
| 2_S | 117_E | 1.418 | 0.99 |
| 215_Y | 280_K | 1.415 | 0.99 |
| 215_Y | 276_L | 1.411 | 0.99 |
| 14_S | 103_I | 1.406 | 0.99 |
| 371_T | 454_I | 1.403 | 0.99 |
| 73_A | 95_I | 1.403 | 0.99 |
| 98_F | 101_Q | 1.399 | 0.99 |
| 94_Q | 98_F | 1.397 | 0.99 |
| 27_I | 110_F | 1.397 | 0.99 |
| 329_V | 337_A | 1.392 | 0.99 |
| 519_A | 549_R | 1.390 | 0.99 |
| 306_K | 309_E | 1.379 | 0.99 |
| 208_M | 281_L | 1.376 | 0.99 |
| 544_E | 547_D | 1.375 | 0.99 |
| 208_M | 322_I | 1.372 | 0.99 |
| 543_N | 547_D | 1.370 | 0.99 |
| 169_K | 195_L | 1.367 | 0.99 |
| 9_L | 100_L | 1.367 | 0.99 |
| 245_A | 257_I | 1.367 | 0.99 |
| 293_K | 328_Y | 1.365 | 0.99 |
| 568_S | 572_Q | 1.363 | 0.99 |
| 423_L | 426_D | 1.358 | 0.99 |
| 136_D | 139_T | 1.357 | 0.99 |
| 283_E | 286_S | 1.347 | 0.99 |
| 73_A | 83_F | 1.345 | 0.99 |
| 150_N | 369_D | 1.332 | 0.99 |
| 265_T | 270_K | 1.331 | 0.99 |
| 379_T | 385_T | 1.331 | 0.99 |
| 519_A | 548_L | 1.331 | 0.99 |
| 297_D | 301_E | 1.328 | 0.99 |
| 384_A | 417_V | 1.325 | 0.99 |
| 195_L | 305_L | 1.324 | 0.99 |
| 522_V | 571_I | 1.324 | 0.99 |
| 215_Y | 284_L | 1.301 | 0.99 |
| 70_I | 95_I | 1.299 | 0.99 |
| 120_I | 138_G | 1.295 | 0.99 |
| 11_T | 178_G | 1.293 | 0.99 |
| 547_D | 563_L | 1.292 | 0.99 |
| 45_A | 61_Q | 1.291 | 0.99 |
| 277_T | 280_K | 1.287 | 0.99 |
| 299_A | 303_A | 1.287 | 0.99 |
| 46_F | 90_F | 1.286 | 0.99 |
| 184_I | 305_L | 1.282 | 0.98 |
| 61_Q | 65_N | 1.280 | 0.98 |
| 169_K | 186_D | 1.279 | 0.98 |
| 439_A | 468_A | 1.274 | 0.98 |
| 44_V | 95_I | 1.265 | 0.98 |
| 146_K | 358_L | 1.263 | 0.98 |
| 198_S | 295_P | 1.256 | 0.98 |
| 219_D | 274_M | 1.255 | 0.98 |
| 417_V | 424_A | 1.254 | 0.98 |
| 484_S | 487_E | 1.253 | 0.98 |
| 9_L | 96_S | 1.249 | 0.98 |
| 285_I | 324_Y | 1.248 | 0.98 |
| 298_K | 302_G | 1.245 | 0.98 |
| 447_Q | 467_K | 1.244 | 0.98 |
| 563_L | 566_K | 1.243 | 0.98 |
| 499_A | 503_R | 1.241 | 0.98 |
| 16_A | 103_I | 1.241 | 0.98 |
| 572_Q | 575_G | 1.239 | 0.98 |
| 538_K | 542_T | 1.235 | 0.98 |
| 73_A | 96_S | 1.235 | 0.98 |
| 289_V | 328_Y | 1.235 | 0.98 |
| 569_K | 573_E | 1.230 | 0.98 |
| 171_L | 333_L | 1.223 | 0.98 |
| 300_L | 305_L | 1.218 | 0.98 |
| 429_S | 432_M | 1.217 | 0.98 |
| 300_L | 307_K | 1.216 | 0.98 |
| 135_K | 459_I | 1.211 | 0.98 |
| 180_L | 292_V | 1.205 | 0.97 |
| 248_E | 255_T | 1.200 | 0.97 |
| 535_K | 542_T | 1.200 | 0.97 |
| 211_A | 285_I | 1.198 | 0.97 |
| 562_T | 566_K | 1.198 | 0.97 |
| 169_K | 305_L | 1.189 | 0.97 |
| 258_D | 273_K | 1.179 | 0.97 |
| 215_Y | 274_M | 1.177 | 0.97 |
| 43_Y | 71_F | 1.173 | 0.97 |
| 298_K | 301_E | 1.169 | 0.97 |
| 75_R | 207_D | 1.168 | 0.97 |
| 208_M | 285_I | 1.167 | 0.97 |
| 212_I | 215_Y | 1.167 | 0.97 |
| 573_E | 577_K | 1.166 | 0.97 |
| 19_V | 356_A | 1.166 | 0.97 |
| 324_Y | 327_K | 1.156 | 0.97 |
| 385_T | 400_Q | 1.143 | 0.96 |
| 150_N | 153_T | 1.142 | 0.96 |
| 65_N | 68_G | 1.137 | 0.96 |
| 74_K | 151_E | 1.134 | 0.96 |
| 24_P | 349_I | 1.129 | 0.96 |
| 511_L | 564_M | 1.127 | 0.96 |
| 104_K | 143_F | 1.122 | 0.96 |
| 572_Q | 576_A | 1.120 | 0.96 |
| 241_A | 255_T | 1.120 | 0.96 |
| 491_M | 494_E | 1.119 | 0.96 |
| 544_E | 548_L | 1.116 | 0.96 |
| 302_G | 390_A | 1.113 | 0.95 |
| 378_E | 415_H | 1.112 | 0.95 |
| 12_S | 177_G | 1.111 | 0.95 |
| 547_D | 566_K | 1.110 | 0.95 |
| 554_E | 559_N | 1.105 | 0.95 |
| 19_V | 24_P | 1.104 | 0.95 |
| 519_A | 545_V | 1.102 | 0.95 |
| 374_T | 420_E | 1.102 | 0.95 |
| 567_L | 570_D | 1.102 | 0.95 |
| 77_M | 134_T | 1.098 | 0.95 |
| 276_L | 281_L | 1.096 | 0.95 |
| 549_R | 553_E | 1.096 | 0.95 |
| 198_S | 298_K | 1.095 | 0.95 |
| 296_I | 329_V | 1.089 | 0.95 |
| 569_K | 572_Q | 1.088 | 0.95 |
| 521_S | 525_T | 1.085 | 0.95 |
| 327_K | 330_E | 1.085 | 0.95 |
| 507_E | 561_K | 1.084 | 0.95 |
| 523_E | 527_N | 1.076 | 0.94 |
| 265_T | 268_G | 1.075 | 0.94 |
| 401_I | 449_E | 1.073 | 0.94 |
| 30_S | 106_D | 1.068 | 0.94 |
| 152_P | 181_D | 1.066 | 0.94 |
| 97_A | 140_I | 1.065 | 0.94 |
| 8_D | 11_T | 1.061 | 0.94 |
| 526_L | 538_K | 1.060 | 0.94 |
| 441_A | 466_D | 1.060 | 0.94 |
| 500_E | 503_R | 1.057 | 0.94 |
| 514_N | 564_M | 1.057 | 0.94 |
| 211_A | 288_I | 1.056 | 0.93 |
| 131_R | 150_N | 1.055 | 0.93 |
| 522_V | 541_L | 1.055 | 0.93 |
| 74_K | 178_G | 1.047 | 0.93 |
| 537_T | 540_R | 1.046 | 0.93 |
| 397_R | 451_T | 1.046 | 0.93 |
| 577_K | 580_Q | 1.043 | 0.93 |
| 305_L | 310_I | 1.042 | 0.93 |
| 411_T | 434_N | 1.042 | 0.93 |
| 198_S | 201_T | 1.042 | 0.93 |
| 522_V | 545_V | 1.040 | 0.93 |
| 106_D | 109_A | 1.037 | 0.93 |
| 26_V | 349_I | 1.035 | 0.93 |
| 560_V | 567_L | 1.027 | 0.92 |
| 278_R | 282_E | 1.026 | 0.92 |
| 217_A | 238_L | 1.024 | 0.92 |
| 566_K | 570_D | 1.024 | 0.92 |
| 544_E | 566_K | 1.020 | 0.92 |
| 151_E | 181_D | 1.019 | 0.92 |
| 305_L | 309_E | 1.017 | 0.92 |
| 544_E | 570_D | 1.017 | 0.92 |
| 286_S | 324_Y | 1.013 | 0.92 |
| 511_L | 561_K | 1.013 | 0.92 |
| 29_S | 32_G | 1.012 | 0.91 |
| 79_T | 82_K | 1.012 | 0.91 |
| 421_R | 502_D | 1.010 | 0.91 |
| 513_N | 520_Y | 1.010 | 0.91 |
| 76_K | 81_Y | 1.008 | 0.91 |
| 6_G | 10_G | 1.007 | 0.91 |
| 38_K | 342_D | 1.006 | 0.91 |
| 297_D | 307_K | 1.005 | 0.91 |
| 565_D | 568_S | 1.005 | 0.91 |
| 256_D | 275_T | 1.004 | 0.91 |
| 220_F | 272_I | 1.002 | 0.91 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3d2fA | 2 | 0.9445 | 100 | 0.151 | Contact Map |
| 4jneA | 2 | 0.9331 | 100 | 0.169 | Contact Map |
| 4fl9A | 1 | 0.8287 | 100 | 0.221 | Contact Map |
| 2v7yA | 1 | 0.8222 | 100 | 0.256 | Contact Map |
| 4j8fA | 1 | 0.832 | 100 | 0.459 | Contact Map |
| 4kboA | 1 | 0.571 | 100 | 0.477 | Contact Map |
| 3qfuA | 1 | 0.571 | 100 | 0.484 | Contact Map |
| 3i33A | 1 | 0.5693 | 100 | 0.489 | Contact Map |
| 4gniA | 2 | 0.5661 | 100 | 0.511 | Contact Map |
| 1jceA | 1 | 0.5253 | 100 | 0.651 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)