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OPENSEQ.org

ClpB

ID: 1402262161 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 857 (855)
Sequences: 3000 (1492.2)
Seq/Len: 3.509
Nf(neff/√len): 51.0

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.509).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
170_E174_Q5.0341.00
796_S847_E4.6331.00
419_I448_L4.2721.00
776_Q800_L4.1501.00
167_D236_R3.9881.00
490_R502_Q3.7501.00
165_T238_L3.6911.00
716_M757_I3.5681.00
772_A801_K3.3871.00
243_G298_M3.3821.00
479_L506_I3.3751.00
412_L455_Y3.3741.00
26_N68_L3.2981.00
346_T374_A3.2591.00
508_E512_Q3.1941.00
496_A500_E3.1111.00
426_Q445_N3.0911.00
423_K452_E3.0591.00
798_E847_E3.0031.00
471_G512_Q2.8561.00
567_H574_N2.8371.00
768_E805_E2.7621.00
166_I171_R2.6551.00
561_R564_Q2.6481.00
343_V374_A2.6461.00
565_E568_H2.6161.00
353_L356_R2.5551.00
832_Q837_E2.5281.00
497_R500_E2.4971.00
602_L757_I2.4731.00
90_V114_A2.4731.00
482_A506_I2.3751.00
780_K783_Y2.3731.00
267_A307_E2.3731.00
159_Q162_K2.3721.00
825_I846_L2.3261.00
486_I506_I2.3151.00
264_N268_K2.2971.00
230_E233_K2.2911.00
479_L513_L2.2761.00
602_L679_V2.2461.00
446_E450_D2.2451.00
36_S65_N2.2381.00
693_V714_V2.1821.00
500_E505_K2.1661.00
339_A669_R2.1541.00
791_Y844_I2.1521.00
488_Q492_V2.1311.00
644_S647_V2.1251.00
27_Q74_V2.1091.00
562_M621_F2.0851.00
720_L745_V2.0791.00
427_Q430_M2.0661.00
50_L55_I2.0441.00
408_K416_D2.0441.00
475_I513_L2.0431.00
567_H577_V2.0411.00
162_K170_E2.0241.00
485_A497_R1.9871.00
587_S592_A1.9851.00
582_N831_Q1.9781.00
577_V614_L1.9711.00
505_K508_E1.9591.00
745_V754_I1.9581.00
402_R406_D1.9481.00
434_D437_S1.9391.00
563_E585_R1.9101.00
463_K467_A1.8981.00
796_S845_R1.8631.00
478_E505_K1.8600.99
483_K510_E1.8540.99
321_Q658_E1.8320.99
481_Q485_A1.8150.99
782_L821_I1.8090.99
477_A481_Q1.7900.99
482_A501_L1.7800.99
203_V280_L1.7790.99
600_S694_L1.7710.99
394_I536_I1.7700.99
370_I394_I1.7700.99
510_E514_E1.7660.99
10_F111_F1.7550.99
690_L693_V1.7510.99
337_F669_R1.7470.99
449_S453_R1.7330.99
104_N150_N1.7240.99
425_E440_R1.7210.99
41_Q44_G1.7160.99
151_D157_Q1.7090.99
640_K685_D1.6940.99
393_L540_L1.6910.99
222_R417_R1.6800.99
478_E512_Q1.6770.99
849_N852_R1.6740.99
838_L844_I1.6740.99
638_M642_S1.6690.99
52_S142_Q1.6650.99
553_E561_R1.6530.99
167_D170_E1.6500.99
408_K459_E1.6450.98
280_L323_I1.6420.98
426_Q441_L1.6400.98
138_Q142_Q1.6400.98
250_K254_E1.6340.98
343_V378_H1.6330.98
459_E463_K1.6320.98
55_I60_L1.6290.98
63_D128_A1.6290.98
482_A505_K1.6270.98
638_M688_N1.6230.98
372_A533_D1.6180.98
234_G272_N1.6130.98
563_E578_D1.6120.98
422_L444_L1.6070.98
453_R457_E1.6070.98
469_L527_L1.6020.98
85_Q89_R1.5900.98
242_M245_L1.5890.98
641_H644_S1.5880.98
720_L744_V1.5870.98
724_L744_V1.5800.98
742_L745_V1.5760.98
474_T477_A1.5750.98
34_L87_L1.5630.98
439_K443_M1.5610.98
226_G233_K1.5590.98
789_R840_P1.5560.98
475_I509_L1.5540.98
575_E738_K1.5420.97
482_A486_I1.5280.97
231_G406_D1.5270.97
803_L820_A1.5210.97
647_V689_I1.5200.97
359_L362_H1.5190.97
569_R613_E1.5170.97
849_N853_I1.5160.97
97_L133_T1.5110.97
775_A804_S1.5110.97
641_H650_P1.5100.97
594_P785_R1.5020.97
30_E84_S1.4970.97
90_V115_A1.4960.97
511_K515_A1.4950.97
39_L64_I1.4940.97
83_P87_L1.4880.97
102_G148_S1.4860.97
280_L312_G1.4830.97
437_S440_R1.4830.97
637_F661_Y1.4800.96
177_L221_Q1.4770.96
666_V709_F1.4650.96
432_E437_S1.4620.96
173_E236_R1.4620.96
411_E454_Q1.4600.96
453_R456_S1.4490.96
284_V329_L1.4470.96
482_A509_L1.4470.96
107_S154_A1.4440.96
418_R447_E1.4400.96
416_D459_E1.4390.96
26_N71_L1.4370.96
779_L795_I1.4350.96
160_A261_G1.4340.96
725_I737_M1.4280.95
107_S149_V1.4270.95
794_H845_R1.4250.95
698_R706_T1.4220.95
514_E518_Q1.4220.95
693_V699_L1.4160.95
40_N61_R1.4130.95
343_V375_T1.4120.95
264_N267_A1.4120.95
9_K12_L1.4010.95
794_H843_V1.3980.95
48_P159_Q1.3940.95
283_M296_G1.3940.95
474_T478_E1.3920.95
742_L812_Y1.3840.94
807_G817_L1.3830.94
615_C715_I1.3820.94
36_S64_I1.3780.94
145_G149_V1.3710.94
348_A421_Q1.3670.94
839_V842_K1.3640.94
137_T140_I1.3570.94
499_S502_Q1.3560.93
342_S345_D1.3510.93
829_L844_I1.3440.93
569_R626_D1.3430.93
430_M445_N1.3430.93
67_A125_I1.3420.93
850_E853_I1.3370.93
779_L821_I1.3320.93
242_M283_M1.3310.93
767_G770_H1.3310.93
197_R591_L1.3290.93
638_M685_D1.3290.93
628_A669_R1.3280.93
802_L848_V1.3250.92
826_E830_A1.3250.92
569_R620_N1.3210.92
617_A620_N1.3070.92
585_R827_N1.3070.92
415_L451_K1.3070.92
736_H740_L1.3040.92
321_Q657_E1.3020.92
602_L745_V1.3020.92
778_Q781_R1.3000.91
153_G156_D1.2950.91
124_D128_A1.2940.91
795_I799_A1.2930.91
65_N69_N1.2930.91
582_N827_N1.2920.91
450_D454_Q1.2920.91
242_M266_L1.2870.91
792_E843_V1.2850.91
630_V672_S1.2850.91
133_T137_T1.2820.91
227_E417_R1.2720.90
478_E481_Q1.2720.90
249_A262_V1.2710.90
190_T336_V1.2650.90
528_R532_T1.2650.90
166_I239_A1.2650.90
199_K392_D1.2620.90
177_L180_V1.2610.90
848_V851_D1.2600.90
480_E484_I1.2590.90
117_E133_T1.2580.90
782_L826_E1.2570.90
32_L71_L1.2520.89
227_E413_D1.2500.89
723_D727_E1.2480.89
100_K137_T1.2460.89
742_L762_V1.2450.89
569_R617_A1.2440.89
24_H73_Q1.2420.89
63_D66_Q1.2420.89
735_A739_E1.2410.89
775_A800_L1.2380.89
364_Q530_K1.2380.89
563_E581_S1.2380.89
734_Y737_M1.2370.89
571_I777_I1.2330.88
495_L504_G1.2300.88
806_N820_A1.2300.88
24_H69_N1.2270.88
820_A824_Q1.2210.88
24_H68_L1.2210.88
246_V298_M1.2200.88
13_A41_Q1.2120.87
418_R421_Q1.2100.87
96_K99_Q1.2070.87
795_I846_L1.1980.86
738_K742_L1.1970.86
223_I237_V1.1970.86
603_F763_F1.1950.86
496_A499_S1.1940.86
679_V757_I1.1930.86
105_F152_Q1.1900.86
482_A485_A1.1900.86
97_L114_A1.1870.86
31_P87_L1.1850.86
203_V323_I1.1830.85
439_K790_G1.1820.85
514_E517_T1.1790.85
97_L100_K1.1790.85
416_D455_Y1.1790.85
46_V136_I1.1780.85
540_L548_V1.1770.85
18_Q81_V1.1760.85
720_L748_N1.1750.85
801_K805_E1.1730.85
207_E316_L1.1650.84
171_R176_K1.1630.84
471_G516_A1.1600.84
3_L14_L1.1600.84
23_G96_K1.1600.84
744_V747_H1.1580.84
630_V666_V1.1580.84
412_L526_L1.1560.84
783_Y793_I1.1560.84
738_K762_V1.1540.83
35_M112_V1.1520.83
44_G47_S1.1460.83
479_L509_L1.1450.83
6_L10_F1.1420.83
776_Q797_D1.1410.82
197_R699_L1.1410.82
32_L67_A1.1410.82
486_I502_Q1.1400.82
36_S40_N1.1380.82
764_H770_H1.1370.82
362_H473_Q1.1360.82
410_E415_L1.1350.82
8_N152_Q1.1340.82
36_S61_R1.1300.82
791_Y838_L1.1290.82
242_M246_V1.1260.81
366_T532_T1.1250.81
559_L585_R1.1250.81
140_I147_E1.1180.81
132_T135_N1.1160.81
637_F645_R1.1150.80
18_Q29_I1.1130.80
20_L37_A1.1100.80
97_L137_T1.1080.80
53_A131_A1.1060.80
168_L239_A1.1060.80
790_G840_P1.1050.80
467_A471_G1.1020.79
592_A598_I1.1020.79
632_I646_L1.1010.79
123_A127_K1.1010.79
785_R826_E1.1000.79
167_D238_L1.1000.79
222_R227_E1.1000.79
561_R565_E1.0990.79
193_V336_V1.0980.79
193_V399_S1.0950.79
775_A821_I1.0950.79
263_L302_A1.0940.79
192_Q400_S1.0910.78
269_Q273_V1.0910.78
565_E617_A1.0900.78
490_R493_G1.0890.78
7_T107_S1.0880.78
560_L834_L1.0880.78
773_S776_Q1.0860.78
160_A265_D1.0860.78
723_D744_V1.0860.78
93_L96_K1.0840.78
511_K514_E1.0820.78
795_I800_L1.0790.77
423_K448_L1.0770.77
424_L427_Q1.0770.77
498_M502_Q1.0740.77
802_L806_N1.0730.77
6_L111_F1.0720.77
317_D321_Q1.0690.76
289_A293_M1.0680.76
849_N854_V1.0660.76
603_F715_I1.0650.76
486_I501_L1.0600.76
221_Q225_N1.0600.76
197_R398_A1.0600.76
189_R192_Q1.0590.75
104_N148_S1.0550.75
489_A494_D1.0520.75
779_L783_Y1.0510.75
429_L441_L1.0500.75
559_L588_R1.0490.74
452_E456_S1.0480.74
126_L129_A1.0470.74
112_V136_I1.0470.74
381_I540_L1.0470.74
320_R649_A1.0460.74
679_V745_V1.0450.74
539_V830_A1.0440.74
479_L482_A1.0430.74
216_V261_G1.0430.74
402_R588_R1.0420.74
593_D753_F1.0410.74
602_L716_M1.0390.73
470_S474_T1.0380.73
405_I469_L1.0340.73
429_L432_E1.0340.73
423_K426_Q1.0330.73
245_L262_V1.0320.73
6_L91_L1.0320.73
57_A61_R1.0310.73
49_L143_M1.0300.73
455_Y459_E1.0290.72
778_Q818_K1.0290.72
745_V760_V1.0270.72
353_L480_E1.0260.72
30_E86_D1.0250.72
60_L125_I1.0250.72
36_S68_L1.0250.72
484_I488_Q1.0240.72
35_M122_L1.0230.72
323_I630_V1.0210.72
517_T577_V1.0190.71
506_I513_L1.0190.71
350_L373_A1.0190.71
302_A551_M1.0190.71
796_S799_A1.0150.71
475_I479_L1.0150.71
13_A34_L1.0140.71
768_E801_K1.0140.71
146_G154_A1.0130.71
494_D497_R1.0130.71
575_E764_H1.0120.71
56_N59_Q1.0110.71
585_R834_L1.0110.71
650_P657_E1.0100.70
419_I451_K1.0060.70
67_A121_T1.0060.70
193_V280_L1.0050.70
461_E524_M1.0030.70
398_A699_L1.0030.70
197_R778_Q1.0030.70
67_A70_R1.0010.69
13_A38_L1.0010.69
566_L617_A1.0000.69
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (29May14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1qvrA30.93351000.455Contact Map
1r6bX10.80631000.5Contact Map
3pxiA30.82961000.53Contact Map
4ciuA10.76551000.557Contact Map
3pxgA60.52161000.741Contact Map
4hseA10.43061000.772Contact Map
3vkgA20.81331000.82Contact Map
4akgA20.79111000.828Contact Map
4lj9A10.38511000.838Contact Map
1g41A10.25791000.885Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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