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OPENSEQ.org
PRPB - Methylisocitrate lyase
UniProt: P77541 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13601
Length: 296 (287)
Sequences: 1621
Seq/Len: 5.65
PRPB
Paralog alert: 0.63 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
71_I 109_A 3.551 1.00
45_S 48_G 3.245 1.00
35_Q 79_S 2.893 1.00
162_L 195_M 2.862 1.00
253_V 257_E 2.583 1.00
252_N 256_Q 2.582 1.00
134_K 176_A 2.492 1.00
197_R 201_D 2.479 1.00
25_T 34_A 2.385 1.00
198_Q 202_A 2.287 1.00
193_L 224_E 2.264 1.00
200_A 207_I 2.235 1.00
168_D 198_Q 2.222 1.00
8_K 152_D 2.195 1.00
86_A 112_L 2.182 1.00
174_A 203_V 2.161 1.00
178_V 205_V 2.151 1.00
165_E 169_A 2.114 1.00
154_V 184_M 2.109 1.00
249_H 266_T 2.058 1.00
193_L 228_A 2.025 1.00
223_D 227_S 2.001 1.00
190_I 199_F 1.964 1.00
19_P 226_R 1.908 1.00
207_I 210_N 1.9 1.00
192_E 195_M 1.893 1.00
35_Q 80_L 1.888 1.00
25_T 42_I 1.861 1.00
169_A 172_E 1.85 1.00
17_E 40_Q 1.794 1.00
145_V 148_K 1.708 1.00
138_V 176_A 1.703 1.00
97_V 144_A 1.698 1.00
171_I 175_Q 1.696 1.00
65_D 69_T 1.694 1.00
168_D 172_E 1.665 1.00
223_D 226_R 1.662 1.00
67_V 104_M 1.637 0.99
84_V 104_M 1.619 0.99
64_L 103_S 1.612 0.99
22_I 232_M 1.611 0.99
83_L 156_M 1.581 0.99
101_V 112_L 1.578 0.99
257_E 262_S 1.576 0.99
9_A 12_A 1.574 0.99
111_G 156_M 1.569 0.99
133_S 136_E 1.563 0.99
12_A 16_K 1.558 0.99
136_E 139_D 1.529 0.99
134_K 177_Y 1.526 0.99
175_Q 202_A 1.516 0.99
278_N 281_Q 1.51 0.99
36_R 248_E 1.452 0.99
22_I 41_A 1.44 0.98
167_L 171_I 1.436 0.98
35_Q 78_C 1.436 0.98
43_Y 113_H 1.401 0.98
194_A 197_R 1.4 0.98
18_N 226_R 1.383 0.98
34_A 251_Y 1.381 0.98
224_E 227_S 1.369 0.98
34_A 42_I 1.365 0.98
175_Q 203_V 1.34 0.97
137_M 177_Y 1.334 0.97
76_D 106_K 1.329 0.97
221_T 224_E 1.329 0.97
170_A 187_P 1.328 0.97
8_K 12_A 1.327 0.97
239_A 251_Y 1.315 0.97
132_V 140_R 1.314 0.97
162_L 167_L 1.309 0.97
39_Y 255_R 1.306 0.97
242_A 250_V 1.294 0.96
105_I 149_T 1.28 0.96
279_Y 283_E 1.271 0.96
75_T 82_L 1.264 0.96
10_F 154_V 1.235 0.95
84_V 109_A 1.234 0.95
91_G 96_N 1.231 0.95
101_V 144_A 1.229 0.95
270_R 274_Y 1.223 0.94
248_E 252_N 1.22 0.94
47_G 51_A 1.217 0.94
220_F 228_A 1.216 0.94
208_L 234_L 1.215 0.94
94_A 136_E 1.206 0.94
10_F 111_G 1.204 0.94
111_G 154_V 1.201 0.94
13_A 40_Q 1.19 0.93
172_E 175_Q 1.189 0.93
17_E 231_A 1.185 0.93
167_L 198_Q 1.18 0.93
271_N 275_E 1.17 0.93
134_K 138_V 1.167 0.92
157_A 182_A 1.158 0.92
141_I 180_A 1.15 0.92
280_Y 283_E 1.145 0.91
134_K 172_E 1.144 0.91
200_A 230_V 1.143 0.91
24_G 43_Y 1.142 0.91
141_I 145_V 1.131 0.91
210_N 214_F 1.127 0.91
125_H 129_K 1.126 0.91
42_I 78_C 1.124 0.90
174_A 187_P 1.121 0.90
136_E 140_R 1.12 0.90
86_A 104_M 1.118 0.90
197_R 228_A 1.107 0.90
138_V 180_A 1.107 0.90
193_L 220_F 1.106 0.90
204_Q 207_I 1.103 0.89
141_I 182_A 1.102 0.89
12_A 15_T 1.098 0.89
170_A 199_F 1.08 0.88
267_M 274_Y 1.074 0.88
16_K 40_Q 1.072 0.87
269_T 272_E 1.061 0.87
118_V 130_A 1.058 0.87
49_V 70_D 1.057 0.86
145_V 155_I 1.055 0.86
40_Q 255_R 1.054 0.86
137_M 140_R 1.05 0.86
94_A 140_R 1.043 0.85
197_R 224_E 1.04 0.85
196_Y 209_A 1.04 0.85
117_Q 123_C 1.03 0.85
272_E 275_E 1.02 0.84
275_E 280_Y 1.016 0.83
200_A 231_A 1.015 0.83
86_A 101_V 1.013 0.83
33_L 251_Y 1.009 0.83
78_C 82_L 1.007 0.83
17_E 226_R 1.006 0.83
94_A 139_D 1.001 0.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3fa4A120.97971000.175Contact Map0.804
3lyeA10.98651000.178Contact Map0.72
1xg4A40.99661000.188Contact Map0.82
3eooA160.99321000.192Contact Map0.79
1s2wA10.9731000.194Contact Map0.634
1zlpA20.99321000.197Contact Map0.694
2hjpA10.95611000.203Contact Map0.637
3i4eA40.97971000.207Contact Map0.691
3eolA20.97971000.21Contact Map0.677
1f8mA40.97971000.217Contact Map0.665
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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