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OPENSEQ.org
NEMA - N-ethylmaleimide reductase
UniProt: P77258 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13546
Length: 365 (357)
Sequences: 5134
Seq/Len: 14.38
NEMA
Paralog alert: 0.73 [within 20: 0.07] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
154_L 203_Q 4.121 1.00
220_D 264_R 3.492 1.00
99_A 178_L 3.283 1.00
207_S 210_N 3.043 1.00
63_S 163_D 2.994 1.00
91_V 98_M 2.946 1.00
121_V 152_L 2.856 1.00
78_P 170_N 2.832 1.00
152_L 160_I 2.826 1.00
40_P 86_K 2.733 1.00
22_F 320_A 2.684 1.00
169_A 225_E 2.659 1.00
165_R 221_A 2.657 1.00
152_L 156_E 2.626 1.00
260_Q 263_K 2.525 1.00
189_L 214_L 2.513 1.00
150_R 156_E 2.428 1.00
11_K 16_T 2.368 1.00
10_L 320_A 2.315 1.00
311_T 315_K 2.273 1.00
210_N 213_R 2.24 1.00
125_A 151_A 2.197 1.00
189_L 215_V 2.179 1.00
7_Y 338_Q 2.174 1.00
215_V 233_I 2.162 1.00
232_G 268_Y 2.156 1.00
335_A 339_R 2.125 1.00
89_A 93_A 2.08 1.00
288_E 317_L 2.073 1.00
85_K 89_A 2.073 1.00
154_L 157_I 2.066 1.00
92_H 177_D 2.024 1.00
169_A 173_E 2.008 1.00
172_R 226_W 1.999 1.00
47_R 94_E 1.989 1.00
14_A 229_D 1.988 1.00
159_G 163_D 1.984 1.00
262_G 295_H 1.946 1.00
310_E 314_G 1.946 1.00
166_Q 170_N 1.933 1.00
204_Y 217_E 1.915 1.00
60_T 100_V 1.914 1.00
330_N 336_R 1.883 1.00
162_N 166_Q 1.872 1.00
165_R 225_E 1.865 1.00
154_L 204_Y 1.864 1.00
183_S 233_I 1.851 1.00
47_R 90_G 1.811 1.00
111_A 147_S 1.782 1.00
81_I 173_E 1.764 1.00
5_K 8_S 1.755 1.00
153_E 156_E 1.737 1.00
212_A 260_Q 1.728 1.00
341_A 365_L 1.725 1.00
88_T 176_F 1.707 1.00
249_N 252_A 1.706 1.00
299_I 322_A 1.696 1.00
259_E 293_R 1.685 1.00
333_L 337_L 1.671 1.00
291_R 319_D 1.646 1.00
215_V 257_L 1.64 1.00
189_L 218_V 1.639 1.00
158_P 217_E 1.634 1.00
172_R 227_G 1.624 1.00
154_L 158_P 1.616 1.00
168_I 222_G 1.596 1.00
346_Q 351_F 1.594 1.00
102_L 167_A 1.591 1.00
65_Q 159_G 1.578 1.00
158_P 203_Q 1.576 1.00
23_M 55_I 1.573 1.00
26_L 324_G 1.569 1.00
288_E 292_A 1.544 1.00
219_V 261_L 1.53 1.00
75_I 100_V 1.517 1.00
78_P 173_E 1.515 1.00
40_P 44_E 1.511 1.00
18_A 97_H 1.51 1.00
43_A 86_K 1.506 1.00
256_Y 260_Q 1.506 1.00
122_A 125_A 1.494 1.00
84_W 176_F 1.482 1.00
44_E 47_R 1.454 1.00
38_P 84_W 1.446 1.00
21_I 334_V 1.434 1.00
57_S 98_M 1.432 1.00
208_V 212_A 1.417 1.00
183_S 189_L 1.409 1.00
23_M 49_R 1.402 0.99
162_N 221_A 1.395 0.99
22_F 299_I 1.38 0.99
221_A 224_E 1.377 0.99
126_L 199_H 1.375 0.99
156_E 159_G 1.37 0.99
68_G 105_T 1.369 0.99
216_L 264_R 1.362 0.99
165_R 224_E 1.352 0.99
213_R 217_E 1.351 0.99
81_I 170_N 1.347 0.99
17_A 54_L 1.344 0.99
48_Q 332_D 1.337 0.99
151_A 201_T 1.33 0.99
309_A 323_F 1.326 0.99
75_I 167_A 1.324 0.99
216_L 260_Q 1.318 0.99
216_L 220_D 1.314 0.99
166_Q 169_A 1.313 0.99
92_H 98_M 1.311 0.99
158_P 204_Y 1.308 0.99
335_A 338_Q 1.305 0.99
195_P 248_P 1.299 0.99
43_A 90_G 1.295 0.99
5_K 314_G 1.292 0.99
289_K 293_R 1.272 0.99
212_A 256_Y 1.271 0.99
5_K 310_E 1.263 0.99
301_A 322_A 1.258 0.99
28_R 41_L 1.248 0.99
312_L 318_I 1.247 0.99
188_L 214_L 1.246 0.98
220_D 224_E 1.238 0.98
347_R 357_E 1.228 0.98
121_V 150_R 1.228 0.98
258_I 269_L 1.227 0.98
83_A 86_K 1.224 0.98
212_A 216_L 1.199 0.98
89_A 92_H 1.197 0.98
310_E 313_I 1.183 0.98
347_R 350_S 1.174 0.98
165_R 169_A 1.172 0.98
327_W 334_V 1.172 0.98
47_R 93_A 1.169 0.97
17_A 22_F 1.169 0.97
161_V 204_Y 1.168 0.97
73_P 84_W 1.167 0.97
126_L 149_P 1.164 0.97
17_A 320_A 1.164 0.97
10_L 297_P 1.161 0.97
221_A 225_E 1.16 0.97
78_P 82_A 1.16 0.97
185_H 272_S 1.157 0.97
34_P 37_I 1.154 0.97
162_N 165_R 1.151 0.97
256_Y 259_E 1.15 0.97
181_L 218_V 1.148 0.97
183_S 215_V 1.146 0.97
119_A 150_R 1.143 0.97
193_L 215_V 1.139 0.97
43_A 87_I 1.135 0.97
88_T 98_M 1.134 0.97
38_P 83_A 1.126 0.97
43_A 47_R 1.118 0.96
190_H 194_S 1.115 0.96
306_V 337_L 1.11 0.96
233_I 261_L 1.102 0.96
161_V 221_A 1.101 0.96
37_I 80_Q 1.099 0.96
32_I 39_T 1.094 0.96
52_A 327_W 1.092 0.96
299_I 320_A 1.092 0.96
85_K 173_E 1.089 0.96
98_M 177_D 1.082 0.96
17_A 53_G 1.081 0.95
57_S 176_F 1.079 0.95
18_A 96_G 1.078 0.95
208_V 256_Y 1.075 0.95
304_Y 312_L 1.074 0.95
77_S 80_Q 1.07 0.95
74_G 80_Q 1.07 0.95
6_L 309_A 1.069 0.95
289_K 292_A 1.066 0.95
75_I 171_A 1.064 0.95
61_Q 72_A 1.058 0.95
97_H 177_D 1.055 0.95
88_T 174_A 1.044 0.94
62_I 164_F 1.042 0.94
121_V 156_E 1.039 0.94
233_I 266_I 1.038 0.94
123_P 197_S 1.036 0.94
190_H 254_A 1.035 0.94
318_I 321_V 1.035 0.94
105_T 109_S 1.028 0.94
81_I 88_T 1.02 0.93
36_D 67_K 1.016 0.93
100_V 171_A 1.013 0.93
163_D 166_Q 1.013 0.93
161_V 217_E 1.012 0.93
51_S 94_E 1.011 0.93
107_R 197_S 1.01 0.93
262_G 296_G 1.008 0.93
306_V 340_K 1.002 0.92
81_I 174_A 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2hsaB20.98361000.135Contact Map0.809
4e2bA10.98631000.139Contact Map0.737
4jicA30.98361000.154Contact Map0.745
3tjlA10.98081000.157Contact Map0.72
4gbuA10.97811000.168Contact Map0.746
4a3uA20.96991000.171Contact Map0.8
1vyrA10.99451000.178Contact Map0.732
4awtA10.9891000.178Contact Map0.768
2r14A10.99181000.182Contact Map0.719
1icpA20.98081000.193Contact Map0.795
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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