OPENSEQ.org - CAS2 - ECOLI GREMLIN

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CAS2 - CRISPR-associated endoribonuclease Cas2

UniProt:	P45956	Sequence MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLPV	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12845
Length:	94 (94)
Sequences:	198
Seq/Len:	2.11

CAS2
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
45_E	49_G	1.968	0.99
34_D	67_G	1.669	0.96
18_R	46_Q	1.613	0.95
14_R	31_Y	1.608	0.95
32_V	59_A	1.606	0.95
7_V	57_V	1.597	0.95
31_Y	84_D	1.566	0.94
79_T	92_L	1.538	0.93
51_A	73_F	1.533	0.93
32_V	68_F	1.496	0.92
15_L	33_G	1.428	0.89
11_V	51_A	1.391	0.87
41_E	71_Q	1.388	0.87
1_M	61_A	1.387	0.87
48_A	73_F	1.384	0.87
39_I	68_F	1.375	0.86
6_V	19_L	1.325	0.84
86_L	94_V	1.314	0.83
22_W	33_G	1.306	0.82
69_E	87_R	1.303	0.82
26_V	47_I	1.292	0.81
14_R	78_R	1.29	0.81
67_G	72_T	1.29	0.81
21_I	66_T	1.284	0.81
26_V	70_F	1.273	0.80
83_L	94_V	1.252	0.79
4_L	26_V	1.241	0.78
39_I	83_L	1.24	0.78
21_I	43_I	1.219	0.76
7_V	30_V	1.202	0.74
48_A	53_E	1.199	0.74
22_W	54_G	1.198	0.74
18_R	21_I	1.192	0.74
10_N	28_A	1.155	0.70
69_E	82_D	1.148	0.70
45_E	71_Q	1.141	0.69
11_V	78_R	1.138	0.69
33_G	65_E	1.133	0.68
10_N	90_S	1.127	0.68
12_P	33_G	1.113	0.66
78_R	91_F	1.072	0.62
82_D	87_R	1.07	0.62
28_A	55_N	1.068	0.61
4_L	23_L	1.068	0.61
38_K	80_P	1.06	0.61
3_M	68_F	1.039	0.58
45_E	52_E	1.038	0.58
15_L	22_W	1.016	0.56
49_G	94_V	1.01	0.55
13_P	89_V	1.003	0.54
20_A	43_I	1.002	0.54

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
4makA	2	1	100	0.024	Contact Map	0.413
4es1A	1	0.9255	64.1	0.899	Contact Map	0.089
3oq2A	2	0.9255	57.2	0.903	Contact Map	0.155
1zpwX	1	0.883	51.5	0.906	Contact Map	0.195
2ivyA	1	0.9362	42.3	0.911	Contact Map	0.072
3excX	1	0.883	24.6	0.921	Contact Map	0.175
2i0xA	1	0.8511	17.5	0.926	Contact Map	0.13
2r0bA	1	0.7234	10.7	0.933	Contact Map	0
3zf7O	1	0.383	7.4	0.938	Contact Map	0
4av2A	12	0.5319	6.2	0.94	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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