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OPENSEQ.org
RLMN - Dual-specificity RNA methyltransferase RlmN
UniProt: P36979 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12401
Length: 384 (352)
Sequences: 1574
Seq/Len: 4.47
RLMN
Paralog alert: 0.05 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
238_N 294_Q 4.175 1.00
58_D 72_K 3.995 1.00
220_D 263_R 3.725 1.00
32_F 45_V 3.552 1.00
185_L 225_M 3.406 1.00
239_D 255_E 3.204 1.00
218_A 221_K 3.022 1.00
283_D 320_G 2.987 1.00
81_E 98_A 2.962 1.00
69_G 73_E 2.774 1.00
130_K 319_Y 2.762 1.00
243_D 249_N 2.682 1.00
224_D 267_K 2.649 1.00
37_E 70_K 2.624 1.00
306_I 339_F 2.623 1.00
332_K 336_S 2.605 1.00
239_D 249_N 2.528 1.00
175_M 191_A 2.491 1.00
27_Q 190_P 2.427 1.00
188_V 210_L 2.417 1.00
126_A 183_L 2.403 1.00
30_E 34_D 2.376 1.00
288_G 291_H 2.365 1.00
95_W 158_I 2.356 1.00
258_L 298_L 2.338 1.00
24_N 27_Q 2.319 1.00
192_M 210_L 2.296 1.00
224_D 263_R 2.223 1.00
29_R 39_P 2.167 1.00
56_N 59_E 2.148 1.00
296_A 337_Y 2.132 1.00
261_V 275_V 2.125 1.00
281_M 322_S 2.12 1.00
237_P 291_H 2.115 1.00
289_T 333_V 2.107 1.00
175_M 210_L 2.038 1.00
193_E 225_M 2.036 1.00
289_T 326_R 2.008 1.00
237_P 294_Q 1.988 1.00
183_L 215_V 1.911 1.00
221_K 225_M 1.911 1.00
31_F 35_L 1.902 1.00
217_P 252_Y 1.888 1.00
127_L 213_S 1.88 1.00
283_D 323_S 1.871 1.00
47_K 62_D 1.864 1.00
238_N 241_I 1.809 1.00
29_R 43_D 1.802 1.00
241_I 291_H 1.795 1.00
262_R 302_T 1.764 1.00
259_A 263_R 1.739 0.99
88_S 350_D 1.719 0.99
86_Q 352_D 1.718 0.99
48_W 60_M 1.697 0.99
55_D 157_K 1.694 0.99
238_N 291_H 1.683 0.99
119_V 148_I 1.682 0.99
182_L 222_L 1.678 0.99
48_W 62_D 1.677 0.99
33_K 39_P 1.672 0.99
293_H 333_V 1.632 0.99
95_W 155_A 1.628 0.99
262_R 301_D 1.616 0.99
83_V 98_A 1.598 0.99
31_F 75_A 1.588 0.99
253_N 256_T 1.587 0.99
173_V 195_M 1.583 0.99
117_L 155_A 1.562 0.99
98_A 103_R 1.522 0.98
175_M 192_M 1.518 0.98
85_E 158_I 1.51 0.98
232_I 257_F 1.49 0.98
126_A 139_F 1.469 0.98
67_L 70_K 1.455 0.98
70_K 74_V 1.445 0.97
41_R 44_Q 1.438 0.97
63_I 68_R 1.431 0.97
21_L 150_G 1.402 0.97
205_K 227_D 1.392 0.97
37_E 67_L 1.39 0.97
134_T 178_M 1.385 0.96
293_H 337_Y 1.377 0.96
291_H 294_Q 1.361 0.96
97_I 106_T 1.361 0.96
323_S 326_R 1.34 0.95
20_L 57_F 1.337 0.95
121_S 188_V 1.333 0.95
327_I 343_V 1.33 0.95
80_P 97_I 1.314 0.95
25_R 29_R 1.31 0.95
54_C 59_E 1.307 0.95
237_P 241_I 1.307 0.95
324_N 328_D 1.303 0.94
152_V 195_M 1.301 0.94
32_F 42_A 1.292 0.94
277_I 302_T 1.287 0.94
196_L 204_S 1.285 0.94
47_K 51_H 1.28 0.94
26_Q 29_R 1.278 0.94
204_S 207_R 1.277 0.94
277_I 304_C 1.269 0.93
40_F 44_Q 1.268 0.93
239_D 243_D 1.261 0.93
101_D 141_R 1.259 0.93
80_P 150_G 1.258 0.93
243_D 250_K 1.244 0.93
25_R 197_D 1.244 0.93
32_F 67_L 1.238 0.92
282_L 311_W 1.233 0.92
175_M 195_M 1.232 0.92
39_P 42_A 1.224 0.92
61_T 68_R 1.222 0.92
69_G 72_K 1.214 0.91
79_A 150_G 1.214 0.91
175_M 188_V 1.212 0.91
37_E 41_R 1.212 0.91
116_T 172_N 1.21 0.91
139_F 183_L 1.203 0.91
196_L 208_V 1.2 0.91
321_R 349_D 1.194 0.90
145_V 190_P 1.194 0.90
45_V 63_I 1.181 0.90
50_Y 201_F 1.18 0.90
108_Y 115_A 1.177 0.90
299_L 306_I 1.177 0.90
352_D 361_D 1.174 0.89
196_L 205_K 1.174 0.89
99_V 104_V 1.164 0.89
148_I 191_A 1.153 0.88
236_A 241_I 1.152 0.88
292_A 333_V 1.152 0.88
43_D 199_F 1.15 0.88
239_D 253_N 1.146 0.88
243_D 246_V 1.14 0.87
23_L 31_F 1.133 0.87
240_E 244_E 1.129 0.87
66_V 69_G 1.125 0.86
43_D 47_K 1.12 0.86
194_I 200_G 1.112 0.86
281_M 327_I 1.11 0.85
137_Q 360_G 1.11 0.85
270_A 273_G 1.104 0.85
185_L 189_V 1.099 0.85
65_K 69_G 1.088 0.84
331_S 335_M 1.085 0.84
232_I 261_V 1.085 0.84
289_T 329_R 1.08 0.83
22_D 146_S 1.079 0.83
25_R 43_D 1.078 0.83
258_L 262_R 1.067 0.82
47_K 52_Y 1.065 0.82
97_I 154_R 1.061 0.82
254_I 298_L 1.058 0.82
19_N 22_D 1.056 0.81
283_D 326_R 1.054 0.81
219_L 260_A 1.051 0.81
328_D 331_S 1.049 0.81
35_L 70_K 1.048 0.81
249_N 253_N 1.045 0.80
277_I 299_L 1.045 0.80
282_L 285_V 1.043 0.80
263_R 267_K 1.04 0.80
134_T 175_M 1.039 0.80
196_L 227_D 1.033 0.79
185_L 193_E 1.031 0.79
216_V 260_A 1.028 0.79
311_W 317_A 1.026 0.79
186_N 221_K 1.023 0.78
96_A 359_A 1.021 0.78
20_L 75_A 1.02 0.78
296_A 339_F 1.017 0.78
156_A 201_F 1.016 0.78
145_V 187_N 1.014 0.78
57_F 76_E 1.002 0.76
258_L 261_V 1 0.76
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3rfaA20.99741000.395Contact Map0.809
2yx0A10.80731000.733Contact Map0.54
2z2uA10.72141000.739Contact Map0.61
2a5hA40.85941000.797Contact Map0.354
3c8fA10.572999.90.831Contact Map0.651
3iixA10.76399.90.856Contact Map0.506
3t7vA10.778699.90.858Contact Map0.423
3canA10.424599.80.868Contact Map0.694
1oltA10.77699.70.882Contact Map0.331
1tv8A20.669399.60.889Contact Map0.673
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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