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OPENSEQ.org
OTSA - Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
UniProt: P31677 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11751
Length: 474 (453)
Sequences: 1172
Seq/Len: 2.59
OTSA
Paralog alert: 0.17 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
224_Y 450_D 3.852 1.00
278_E 320_R 3.517 1.00
115_D 144_R 3.119 1.00
284_Y 414_D 3.032 1.00
258_I 413_L 3.023 1.00
282_E 324_K 2.801 1.00
399_L 419_M 2.699 1.00
356_L 413_L 2.661 1.00
135_L 177_L 2.6 1.00
185_F 194_F 2.58 1.00
171_D 175_E 2.55 1.00
423_E 427_R 2.496 1.00
414_D 418_T 2.404 1.00
278_E 324_K 2.268 1.00
72_D 108_R 2.211 1.00
278_E 325_Y 2.186 1.00
382_V 399_L 2.13 1.00
176_Q 179_D 2.023 1.00
116_K 119_P 2.023 1.00
182_L 224_Y 2.006 0.99
405_D 408_E 1.975 0.99
282_E 325_Y 1.963 0.99
118_L 141_L 1.92 0.99
274_F 333_L 1.915 0.99
256_Q 417_L 1.906 0.99
174_L 197_C 1.895 0.99
277_Y 281_L 1.831 0.99
419_M 423_E 1.824 0.99
277_Y 293_Y 1.814 0.99
280_L 287_H 1.814 0.99
260_S 295_Q 1.79 0.99
137_F 141_L 1.788 0.98
319_G 323_G 1.782 0.98
312_H 316_N 1.76 0.98
184_G 224_Y 1.732 0.98
139_H 175_E 1.692 0.98
111_A 140_E 1.69 0.98
244_K 247_Q 1.684 0.98
11_I 65_S 1.682 0.98
80_F 169_T 1.68 0.98
288_H 325_Y 1.678 0.97
170_Y 387_Q 1.636 0.97
72_D 112_L 1.618 0.97
369_K 394_E 1.617 0.97
275_L 320_R 1.617 0.97
270_L 314_L 1.615 0.97
151_G 447_F 1.605 0.96
397_S 434_V 1.602 0.96
186_Q 190_D 1.58 0.96
395_L 431_M 1.578 0.96
142_R 150_I 1.578 0.96
27_G 226_I 1.572 0.96
138_A 180_Y 1.571 0.96
275_L 278_E 1.566 0.96
277_Y 291_I 1.552 0.95
111_A 115_D 1.534 0.95
375_Q 424_R 1.523 0.95
388_F 402_N 1.513 0.95
354_V 384_V 1.511 0.95
151_G 451_L 1.511 0.95
382_V 419_M 1.504 0.94
428_H 432_L 1.503 0.94
11_I 41_G 1.501 0.94
354_V 416_A 1.488 0.94
111_A 144_R 1.483 0.94
274_F 321_I 1.48 0.94
56_V 60_N 1.477 0.94
89_F 135_L 1.459 0.93
234_A 238_A 1.447 0.93
6_V 29_L 1.444 0.93
261_V 345_L 1.426 0.92
356_L 409_V 1.425 0.92
421_L 425_I 1.424 0.92
280_L 284_Y 1.419 0.92
397_S 430_E 1.407 0.91
193_A 196_D 1.399 0.91
274_F 318_A 1.396 0.91
152_F 177_L 1.388 0.91
287_H 414_D 1.387 0.91
71_Q 75_E 1.382 0.90
107_L 172_T 1.374 0.90
305_Q 309_D 1.37 0.90
3_R 125_D 1.367 0.90
127_I 148_N 1.36 0.89
442_H 446_C 1.353 0.89
15_D 46_T 1.352 0.89
72_D 105_G 1.343 0.89
154_L 158_F 1.342 0.89
245_L 351_Y 1.342 0.89
399_L 412_A 1.341 0.89
64_A 120_L 1.339 0.88
289_G 330_W 1.337 0.88
195_L 221_T 1.329 0.88
192_L 196_D 1.319 0.87
145_G 148_N 1.308 0.87
256_Q 353_D 1.308 0.87
174_L 202_T 1.307 0.87
89_F 154_L 1.301 0.86
91_Y 361_R 1.3 0.86
78_N 303_D 1.298 0.86
75_E 105_G 1.295 0.86
314_L 318_A 1.284 0.85
315_E 319_G 1.283 0.85
139_H 143_K 1.281 0.85
65_S 158_F 1.278 0.85
268_K 362_D 1.275 0.85
89_F 156_I 1.273 0.85
181_D 217_K 1.271 0.85
136_P 176_Q 1.261 0.84
82_N 301_R 1.257 0.84
345_L 349_F 1.257 0.84
127_I 145_G 1.256 0.84
275_L 317_E 1.251 0.83
268_K 365_N 1.242 0.83
82_N 307_Y 1.241 0.83
431_M 435_I 1.23 0.82
258_I 356_L 1.225 0.82
41_G 154_L 1.214 0.81
70_E 74_D 1.214 0.81
283_K 411_A 1.212 0.81
77_Y 82_N 1.211 0.81
250_A 253_K 1.21 0.80
307_Y 367_V 1.21 0.80
392_A 400_I 1.205 0.80
258_I 291_I 1.198 0.80
263_R 362_D 1.193 0.79
41_G 65_S 1.189 0.79
191_R 221_T 1.188 0.79
419_M 427_R 1.181 0.78
88_A 96_V 1.181 0.78
265_D 268_K 1.18 0.78
82_N 263_R 1.18 0.78
268_K 307_Y 1.171 0.77
177_L 248_L 1.17 0.77
273_R 295_Q 1.167 0.77
425_I 429_A 1.162 0.77
175_E 202_T 1.155 0.76
122_Q 125_D 1.148 0.75
278_E 317_E 1.148 0.75
127_I 150_I 1.148 0.75
140_E 179_D 1.148 0.75
362_D 365_N 1.145 0.75
322_N 333_L 1.141 0.75
5_V 121_L 1.136 0.74
290_K 422_A 1.132 0.74
81_S 133_H 1.129 0.74
107_L 111_A 1.127 0.73
257_N 351_Y 1.122 0.73
316_N 320_R 1.117 0.73
87_P 269_G 1.116 0.72
162_E 193_A 1.115 0.72
279_A 282_E 1.115 0.72
111_A 143_K 1.112 0.72
242_P 347_K 1.112 0.72
77_Y 86_W 1.111 0.72
276_A 409_V 1.111 0.72
177_L 183_L 1.111 0.72
268_K 301_R 1.11 0.72
401_V 409_V 1.109 0.72
228_I 300_S 1.107 0.72
311_R 335_Y 1.107 0.72
268_K 367_V 1.105 0.71
10_R 23_G 1.101 0.71
135_L 152_F 1.098 0.71
433_D 437_K 1.097 0.71
279_A 407_D 1.093 0.70
32_L 37_G 1.092 0.70
138_A 146_V 1.091 0.70
118_L 144_R 1.085 0.69
354_V 417_L 1.081 0.69
41_G 44_G 1.08 0.69
286_Q 414_D 1.072 0.68
27_G 260_S 1.07 0.68
121_L 124_D 1.07 0.68
289_G 329_G 1.07 0.68
121_L 148_N 1.068 0.68
105_G 108_R 1.066 0.67
191_R 223_V 1.062 0.67
162_E 189_N 1.061 0.67
195_L 378_A 1.059 0.67
301_R 304_V 1.055 0.66
278_E 333_L 1.054 0.66
228_I 233_I 1.051 0.66
170_Y 197_C 1.045 0.65
213_T 216_G 1.045 0.65
225_P 265_D 1.045 0.65
178_C 194_F 1.044 0.65
233_I 346_M 1.044 0.65
106_Y 110_N 1.042 0.65
301_R 307_Y 1.042 0.65
222_E 431_M 1.041 0.65
182_L 220_R 1.037 0.64
3_R 122_Q 1.035 0.64
69_S 72_D 1.035 0.64
315_E 395_L 1.032 0.64
9_N 301_R 1.03 0.64
167_L 258_I 1.027 0.63
140_E 176_Q 1.025 0.63
38_L 64_A 1.024 0.63
87_P 128_W 1.021 0.63
93_L 166_A 1.016 0.62
269_G 295_Q 1.016 0.62
152_F 158_F 1.015 0.62
9_N 24_L 1.015 0.62
311_R 315_E 1.008 0.61
230_P 435_I 1.005 0.61
240_P 246_A 1.005 0.61
419_M 424_R 1.003 0.60
264_L 335_Y 1.002 0.60
240_P 422_A 1.001 0.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1uqtA211000.35Contact Map0.732
3t5tA20.96411000.412Contact Map0.58
3nb0A40.936799.90.864Contact Map0.437
2r60A10.875599.80.879Contact Map0.505
3vueA10.894599.80.883Contact Map0.488
3froA30.839799.70.887Contact Map0.488
3c48A20.835499.70.889Contact Map0.624
3s28A80.911499.70.89Contact Map0.523
3okpA10.780699.70.892Contact Map0.574
2x6qA20.835499.70.893Contact Map0.611
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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