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OPENSEQ.org
MNME - tRNA modification GTPase MnmE
UniProt: P25522 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10997
Length: 454 (451)
Sequences: 1707
Seq/Len: 3.78
MNME
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
284_I 287_E 3.812 1.00
46_A 65_L 3.7 1.00
20_G 90_L 3.621 1.00
73_F 112_S 3.564 1.00
32_V 103_L 3.209 1.00
8_V 94_L 3.153 1.00
5_D 104_R 3.102 1.00
124_A 153_S 2.873 1.00
75_G 120_K 2.755 1.00
176_F 452_I 2.739 1.00
28_K 31_E 2.697 1.00
107_R 113_E 2.627 1.00
201_D 400_A 2.571 1.00
70_P 77_D 2.448 1.00
13_P 17_G 2.437 1.00
246_D 280_E 2.414 1.00
112_S 129_I 2.408 1.00
197_I 404_Q 2.395 1.00
162_A 195_D 2.318 1.00
65_L 82_Q 2.259 1.00
247_I 279_D 2.233 1.00
30_R 66_W 2.23 1.00
197_I 400_A 2.223 1.00
302_M 335_N 2.218 1.00
371_H 374_Q 2.154 1.00
300_L 333_V 2.144 1.00
200_L 396_L 2.143 1.00
62_G 81_L 2.136 1.00
262_D 373_K 2.114 1.00
394_Q 398_Q 2.095 1.00
73_F 129_I 2.09 1.00
8_V 97_I 2.082 1.00
298_R 372_L 2.076 1.00
235_A 365_V 2.027 1.00
42_K 45_Y 2.018 1.00
168_I 445_R 1.983 1.00
16_R 134_D 1.978 1.00
193_L 403_L 1.937 1.00
37_L 49_L 1.929 1.00
48_Y 61_Q 1.911 1.00
6_T 28_K 1.874 1.00
233_L 242_A 1.802 0.99
217_M 376_M 1.793 0.99
96_R 101_P 1.791 0.99
194_N 407_K 1.778 0.99
172_A 176_F 1.764 0.99
57_S 142_R 1.764 0.99
24_I 81_L 1.754 0.99
254_V 269_I 1.748 0.99
6_T 29_A 1.746 0.99
24_I 33_A 1.744 0.99
222_A 293_I 1.735 0.99
61_Q 120_K 1.733 0.99
287_E 291_Q 1.729 0.99
137_S 260_H 1.713 0.99
402_H 422_E 1.712 0.99
88_V 141_A 1.682 0.99
333_V 355_I 1.677 0.99
197_I 407_K 1.674 0.99
24_I 97_I 1.659 0.99
6_T 32_V 1.655 0.99
160_V 164_T 1.653 0.99
29_A 79_L 1.644 0.99
190_E 411_L 1.631 0.99
131_D 214_R 1.624 0.99
147_S 388_A 1.617 0.99
97_I 103_L 1.609 0.98
132_L 141_A 1.581 0.98
38_G 52_K 1.572 0.98
197_I 201_D 1.567 0.98
22_L 94_L 1.565 0.98
406_G 419_L 1.557 0.98
35_T 101_P 1.546 0.98
291_Q 295_Q 1.538 0.98
241_A 257_E 1.534 0.98
243_I 284_I 1.529 0.98
21_I 82_Q 1.526 0.98
389_R 392_H 1.523 0.98
162_A 199_D 1.513 0.97
72_S 75_G 1.508 0.97
157_N 436_E 1.504 0.97
402_H 425_L 1.503 0.97
63_I 67_F 1.502 0.97
442_L 446_I 1.495 0.97
398_Q 432_E 1.476 0.97
197_I 403_L 1.47 0.97
127_E 391_R 1.46 0.97
305_G 337_A 1.45 0.96
69_G 77_D 1.45 0.96
46_A 63_I 1.417 0.96
132_L 145_L 1.416 0.96
245_T 281_V 1.405 0.96
288_R 292_E 1.402 0.95
59_L 96_R 1.401 0.95
450_F 453_G 1.397 0.95
33_A 64_A 1.392 0.95
331_T 371_H 1.391 0.95
237_A 241_A 1.387 0.95
6_T 27_F 1.386 0.95
445_R 449_S 1.385 0.95
51_F 62_G 1.384 0.95
121_L 129_I 1.38 0.95
171_E 424_R 1.372 0.95
395_A 429_N 1.369 0.95
43_P 66_W 1.364 0.95
408_A 412_G 1.356 0.94
370_N 374_Q 1.348 0.94
8_V 98_L 1.345 0.94
129_I 174_I 1.343 0.94
398_Q 429_N 1.333 0.94
291_Q 294_E 1.33 0.94
162_A 192_Q 1.312 0.93
33_A 79_L 1.308 0.93
201_D 404_Q 1.308 0.93
298_R 331_T 1.306 0.93
279_D 286_I 1.304 0.93
225_P 248_A 1.303 0.93
98_L 105_I 1.302 0.93
400_A 404_Q 1.295 0.92
405_Q 422_E 1.294 0.92
96_R 99_T 1.293 0.92
83_G 93_L 1.286 0.92
165_H 192_Q 1.283 0.92
10_Q 94_L 1.283 0.92
131_D 392_H 1.28 0.92
283_R 287_E 1.276 0.92
429_N 432_E 1.275 0.92
81_L 93_L 1.274 0.91
62_G 83_G 1.273 0.91
409_Q 422_E 1.267 0.91
292_E 295_Q 1.265 0.91
200_L 403_L 1.259 0.91
394_Q 397_E 1.253 0.91
43_P 68_P 1.241 0.90
10_Q 90_L 1.238 0.90
210_G 392_H 1.235 0.90
254_V 292_E 1.232 0.90
50_P 58_V 1.232 0.90
219_V 298_R 1.224 0.89
277_A 286_I 1.224 0.89
59_L 93_L 1.222 0.89
54_A 96_R 1.211 0.88
130_A 134_D 1.209 0.88
281_V 284_I 1.204 0.88
7_I 12_T 1.2 0.88
322_A 325_P 1.197 0.88
401_E 405_Q 1.194 0.88
131_D 389_R 1.19 0.87
172_A 452_I 1.189 0.87
161_E 445_R 1.187 0.87
31_E 35_T 1.183 0.87
147_S 392_H 1.182 0.87
200_L 399_A 1.181 0.87
204_R 397_E 1.179 0.87
211_S 239_R 1.169 0.86
67_F 78_V 1.166 0.86
30_R 68_P 1.164 0.86
201_D 205_A 1.16 0.85
155_R 199_D 1.159 0.85
451_C 454_K 1.158 0.85
218_K 221_I 1.155 0.85
218_K 267_H 1.147 0.85
202_A 366_D 1.146 0.84
36_V 97_I 1.145 0.84
402_H 405_Q 1.144 0.84
75_G 121_L 1.143 0.84
53_D 57_S 1.142 0.84
156_V 430_L 1.14 0.84
219_V 266_L 1.134 0.84
337_A 356_R 1.133 0.84
169_Y 173_A 1.131 0.83
262_D 370_N 1.131 0.83
364_G 368_L 1.131 0.83
431_S 437_F 1.13 0.83
30_R 79_L 1.13 0.83
126_A 129_I 1.129 0.83
190_E 407_K 1.129 0.83
53_D 99_T 1.126 0.83
10_Q 20_G 1.123 0.83
237_A 257_E 1.12 0.83
167_R 427_Q 1.119 0.82
450_F 454_K 1.119 0.82
277_A 280_E 1.118 0.82
90_L 93_L 1.114 0.82
231_S 359_A 1.11 0.82
18_G 78_V 1.107 0.82
335_N 359_A 1.104 0.81
158_H 199_D 1.104 0.81
64_A 81_L 1.102 0.81
29_A 33_A 1.101 0.81
44_R 66_W 1.096 0.81
64_A 79_L 1.091 0.80
238_G 369_R 1.09 0.80
66_W 69_G 1.083 0.80
41_P 47_D 1.082 0.79
137_S 265_P 1.071 0.79
235_A 359_A 1.069 0.78
358_S 361_T 1.068 0.78
18_G 245_T 1.065 0.78
170_V 420_A 1.064 0.78
204_R 400_A 1.062 0.78
387_L 396_L 1.058 0.77
261_I 369_R 1.054 0.77
62_G 65_L 1.052 0.77
233_L 241_A 1.047 0.76
99_T 397_E 1.045 0.76
163_L 423_L 1.044 0.76
400_A 403_L 1.044 0.76
361_T 364_G 1.04 0.76
256_R 267_H 1.04 0.76
57_S 92_L 1.032 0.75
44_R 71_N 1.031 0.75
8_V 24_I 1.027 0.74
194_N 197_I 1.026 0.74
32_V 97_I 1.022 0.74
11_A 72_S 1.022 0.74
29_A 32_V 1.019 0.74
176_F 424_R 1.014 0.73
260_H 263_G 1.013 0.73
364_G 367_V 1.011 0.73
188_K 192_Q 1.007 0.72
358_S 364_G 1.005 0.72
156_V 203_V 1.003 0.72
91_D 390_R 1 0.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1xzpA10.98021000.208Contact Map0.607
3geeA10.98461000.219Contact Map0.648
3gehA10.98461000.221Contact Map0.618
2e87A10.70931000.783Contact Map0.381
2qm8A20.59691000.79Contact Map0.431
4b43A10.64761000.8Contact Map0.463
2wkqA10.66521000.804Contact Map0.404
1egaA20.51981000.805Contact Map0.408
2wwwA40.62331000.806Contact Map0.388
2j69A40.64981000.806Contact Map0.598
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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