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OPENSEQ.org
METF - 5,10-methylenetetrahydrofolate reductase
UniProt: P0AEZ1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10585
Length: 296 (275)
Sequences: 1825
Seq/Len: 6.64
METF
Paralog alert: 0.12 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
173_V 205_V 4.155 1.00
233_A 237_Q 3.058 1.00
148_S 179_R 3.037 1.00
170_K 174_D 2.945 1.00
136_V 149_V 2.919 1.00
192_L 267_E 2.901 1.00
188_V 260_M 2.825 1.00
151_A 180_A 2.802 1.00
258_M 289_T 2.792 1.00
168_N 171_R 2.685 1.00
198_C 207_I 2.676 1.00
103_A 139_L 2.56 1.00
192_L 263_I 2.531 1.00
195_R 199_V 2.404 1.00
261_V 274_F 2.34 1.00
116_A 139_L 2.198 1.00
274_F 283_S 2.091 1.00
262_K 266_R 2.083 1.00
149_V 180_A 2.044 1.00
100_R 138_L 2.038 1.00
166_L 201_A 1.993 1.00
248_T 251_L 1.992 1.00
184_F 224_A 1.937 1.00
235_M 256_I 1.904 1.00
163_Q 197_R 1.881 1.00
84_E 113_H 1.868 1.00
238_M 259_D 1.841 1.00
251_L 285_A 1.834 1.00
261_V 272_F 1.815 1.00
78_K 85_A 1.808 1.00
113_H 146_D 1.805 1.00
134_D 137_T 1.79 1.00
196_D 200_S 1.766 1.00
34_T 37_M 1.697 1.00
170_K 203_I 1.685 1.00
86_A 179_R 1.685 1.00
133_S 171_R 1.683 1.00
189_E 193_R 1.65 1.00
242_L 252_V 1.625 1.00
198_C 203_I 1.624 1.00
195_R 267_E 1.603 1.00
188_V 263_I 1.589 1.00
169_L 180_A 1.584 1.00
75_K 79_D 1.569 0.99
255_N 289_T 1.559 0.99
219_Q 223_F 1.557 0.99
51_L 284_Y 1.549 0.99
137_T 141_E 1.543 0.99
193_R 197_R 1.534 0.99
211_I 257_A 1.517 0.99
149_V 177_A 1.511 0.99
133_S 137_T 1.499 0.99
255_N 259_D 1.495 0.99
191_Y 269_V 1.493 0.99
155_V 162_A 1.419 0.99
194_F 198_C 1.418 0.99
167_L 171_R 1.407 0.99
86_A 115_V 1.392 0.99
78_K 84_E 1.387 0.98
195_R 207_I 1.387 0.98
24_N 271_D 1.387 0.98
167_L 201_A 1.382 0.98
96_P 129_E 1.378 0.98
136_V 147_I 1.373 0.98
146_D 179_R 1.366 0.98
191_Y 195_R 1.364 0.98
104_R 108_N 1.361 0.98
113_H 179_R 1.36 0.98
170_K 201_A 1.357 0.98
38_E 42_W 1.346 0.98
26_S 55_F 1.321 0.98
180_A 205_V 1.31 0.98
161_S 164_A 1.309 0.98
95_T 129_E 1.304 0.97
260_M 263_I 1.303 0.97
78_K 112_R 1.289 0.97
46_D 49_S 1.285 0.97
210_G 275_Y 1.28 0.97
103_A 145_F 1.279 0.97
173_V 204_D 1.278 0.97
140_K 178_N 1.272 0.97
25_V 287_C 1.268 0.97
107_W 112_R 1.249 0.96
258_M 286_I 1.244 0.96
254_A 289_T 1.238 0.96
89_L 114_I 1.236 0.96
104_R 142_V 1.221 0.96
187_D 230_R 1.218 0.96
212_L 222_K 1.215 0.96
221_K 240_D 1.213 0.96
146_D 178_N 1.205 0.95
71_H 106_Y 1.197 0.95
46_D 80_R 1.195 0.95
188_V 192_L 1.193 0.95
169_L 203_I 1.19 0.95
285_A 289_T 1.187 0.95
285_A 288_H 1.185 0.95
189_E 263_I 1.176 0.95
253_G 257_A 1.17 0.94
47_R 284_Y 1.169 0.94
238_M 252_V 1.144 0.94
262_K 289_T 1.14 0.93
250_K 281_E 1.139 0.93
147_I 178_N 1.124 0.93
76_G 80_R 1.123 0.93
164_A 167_L 1.119 0.93
152_Y 165_D 1.109 0.92
193_R 196_D 1.105 0.92
99_L 102_I 1.104 0.92
133_S 175_A 1.103 0.92
222_K 226_M 1.101 0.92
237_Q 240_D 1.099 0.92
151_A 182_T 1.097 0.92
42_W 76_G 1.096 0.91
23_I 265_S 1.086 0.91
192_L 195_R 1.083 0.91
171_R 175_A 1.083 0.91
160_K 164_A 1.077 0.91
263_I 267_E 1.073 0.90
220_A 231_I 1.073 0.90
37_M 41_L 1.067 0.90
113_H 148_S 1.058 0.89
199_V 204_D 1.057 0.89
245_D 248_T 1.054 0.89
36_E 39_Q 1.053 0.89
74_I 87_P 1.052 0.89
284_Y 288_H 1.047 0.89
104_R 143_A 1.046 0.89
117_L 150_A 1.045 0.89
58_V 74_I 1.041 0.88
105_D 108_N 1.039 0.88
182_T 191_Y 1.039 0.88
187_D 260_M 1.033 0.88
173_V 180_A 1.023 0.87
56_V 83_L 1.018 0.87
49_S 82_G 1.017 0.87
89_L 102_I 1.01 0.86
24_N 54_K 1.008 0.86
184_F 223_F 1.003 0.86
30_F 33_R 1.003 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3fstA311000.076Contact Map0.79
3aptA20.95951000.095Contact Map0.761
3ijdA20.93241000.273Contact Map0.548
3navA20.8041950.925Contact Map0.534
1qopA10.7973950.925Contact Map0.518
3vndA80.787294.80.926Contact Map0.54
2p10A60.763594.50.927Contact Map0.536
1rd5A20.726493.30.93Contact Map0.454
1vc4A20.739992.90.931Contact Map0.513
1geqA20.692692.60.932Contact Map0.485
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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