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OPENSEQ.org
HEMG - Protoporphyrinogen IX dehydrogenase [menaquinone]
UniProt: P0ACB4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11485
Length: 181 (174)
Sequences: 349
Seq/Len: 2.01
HEMG
Paralog alert: 0.03 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
33_D 48_Y 3.575 1.00
52_V 171_I 2.343 1.00
54_G 85_Y 2.257 1.00
24_E 165_A 2.22 1.00
44_Q 47_N 2.023 0.99
11_D 135_D 1.935 0.99
24_E 169_R 1.872 0.98
85_Y 122_I 1.841 0.98
20_Y 165_A 1.822 0.98
5_I 18_A 1.74 0.97
76_R 80_M 1.615 0.95
89_L 126_L 1.577 0.94
83_A 167_F 1.557 0.93
11_D 58_R 1.524 0.92
37_V 68_E 1.522 0.92
137_F 140_K 1.499 0.91
6_L 37_V 1.473 0.90
9_T 157_V 1.366 0.85
125_A 155_E 1.347 0.84
13_Q 157_V 1.346 0.84
59_Y 138_M 1.311 0.82
16_E 157_V 1.31 0.82
159_T 164_V 1.303 0.81
50_R 81_P 1.291 0.80
131_Y 167_F 1.283 0.80
55_A 63_H 1.283 0.80
152_T 156_V 1.277 0.79
97_R 101_T 1.271 0.79
64_S 144_K 1.239 0.76
74_A 78_N 1.22 0.75
132_R 137_F 1.216 0.74
139_I 142_I 1.213 0.74
50_R 57_I 1.211 0.74
130_R 155_E 1.207 0.74
165_A 168_A 1.202 0.73
62_Y 122_I 1.201 0.73
18_A 50_R 1.199 0.73
51_V 80_M 1.198 0.73
60_G 104_Y 1.193 0.72
36_N 39_R 1.189 0.72
149_E 154_K 1.176 0.71
62_Y 86_S 1.164 0.70
29_G 74_A 1.162 0.69
21_L 85_Y 1.148 0.68
8_S 122_I 1.148 0.68
83_A 171_I 1.142 0.68
59_Y 123_A 1.137 0.67
9_T 19_S 1.131 0.66
149_E 155_E 1.129 0.66
3_T 50_R 1.125 0.66
140_K 152_T 1.123 0.66
17_I 87_V 1.119 0.65
129_P 135_D 1.117 0.65
95_E 141_L 1.114 0.65
33_D 162_E 1.11 0.64
7_F 15_R 1.109 0.64
18_A 22_A 1.103 0.64
52_V 76_R 1.098 0.63
52_V 83_A 1.094 0.63
41_E 74_A 1.09 0.62
110_M 115_R 1.084 0.62
8_S 63_H 1.082 0.61
166_N 170_E 1.081 0.61
4_L 160_D 1.072 0.60
2_K 31_Q 1.069 0.60
84_F 108_F 1.064 0.59
84_F 116_P 1.059 0.59
13_Q 159_T 1.055 0.59
35_A 47_N 1.054 0.58
143_M 148_G 1.052 0.58
79_S 173_H 1.048 0.58
55_A 114_W 1.047 0.58
83_A 118_R 1.046 0.58
56_S 63_H 1.045 0.57
91_A 102_N 1.043 0.57
125_A 131_Y 1.041 0.57
86_S 105_A 1.037 0.57
41_E 44_Q 1.031 0.56
82_S 117_D 1.025 0.55
126_L 155_E 1.025 0.55
36_N 72_K 1.023 0.55
160_D 163_Q 1.023 0.55
41_E 72_K 1.019 0.55
155_E 158_Y 1.015 0.54
89_L 125_A 1.014 0.54
62_Y 66_F 1.006 0.53
24_E 168_A 1.005 0.53
49_D 80_M 1.004 0.53
139_I 148_G 1.002 0.53
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ykgA10.801199.70.692Contact Map0.41
4h2dA20.823299.70.695Contact Map0.528
1yobA20.933799.70.696Contact Map0.405
2wc1A10.933799.70.698Contact Map0.354
1bvyF10.812299.70.698Contact Map0.474
1cznA10.922799.70.704Contact Map0.564
1f4pA10.784599.70.705Contact Map0.66
1oboA20.917199.70.705Contact Map0.546
3f6rA40.784599.60.709Contact Map0.583
2hnaA10.784599.60.711Contact Map0.311
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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