May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
PHEA - P-protein
UniProt: P0A9J8 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10707
Length: 386 (378)
Sequences: 583
Seq/Len: 1.54
PHEA
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
13_K 27_E 2.86 1.00
20_K 23_A 2.815 1.00
320_N 361_E 2.759 1.00
318_L 325_M 2.754 1.00
142_I 154_A 2.668 1.00
302_M 344_L 2.569 1.00
154_A 283_L 2.505 1.00
255_E 268_R 2.361 1.00
7_L 35_V 2.343 1.00
193_L 253_G 2.276 0.99
203_I 250_A 2.262 0.99
115_Y 190_H 2.211 0.99
316_L 320_N 2.204 0.99
20_K 27_E 2.203 0.99
208_S 234_T 2.149 0.99
34_E 37_K 2.133 0.99
307_Q 313_E 2.124 0.99
107_A 151_A 2.107 0.99
114_S 235_S 2.087 0.99
23_A 27_E 2 0.98
321_H 357_K 1.96 0.98
300_L 346_I 1.926 0.97
109_L 233_S 1.911 0.97
208_S 212_P 1.871 0.97
252_L 270_E 1.823 0.96
22_L 59_L 1.814 0.96
236_A 239_E 1.795 0.96
321_H 361_E 1.793 0.96
143_F 154_A 1.758 0.95
212_P 234_T 1.754 0.95
190_H 216_C 1.743 0.95
204_N 248_H 1.736 0.95
325_M 346_I 1.735 0.95
258_G 265_V 1.734 0.95
10_L 31_L 1.733 0.95
22_L 73_I 1.704 0.94
205_T 227_K 1.688 0.93
111_P 233_S 1.676 0.93
360_K 363_G 1.671 0.93
55_L 81_I 1.67 0.93
147_E 178_L 1.628 0.92
207_Y 229_E 1.589 0.90
171_D 331_R 1.567 0.90
299_T 375_Y 1.559 0.89
194_V 198_T 1.549 0.89
189_D 271_A 1.532 0.88
240_K 244_A 1.525 0.88
13_K 34_E 1.51 0.87
292_D 351_E 1.506 0.87
32_A 84_S 1.504 0.87
198_T 202_T 1.488 0.86
223_Y 226_W 1.486 0.86
353_A 357_K 1.473 0.85
180_I 375_Y 1.467 0.85
30_E 33_V 1.461 0.85
27_E 30_E 1.414 0.82
206_V 330_S 1.411 0.82
325_M 358_A 1.411 0.82
345_D 375_Y 1.407 0.82
13_K 31_L 1.4 0.81
355_M 358_A 1.398 0.81
63_G 66_H 1.398 0.81
46_V 85_V 1.398 0.81
321_H 325_M 1.393 0.81
139_F 169_V 1.385 0.80
244_A 248_H 1.382 0.80
311_L 328_L 1.369 0.79
48_D 81_I 1.36 0.78
11_R 51_R 1.354 0.78
17_L 31_L 1.349 0.78
183_E 297_K 1.348 0.78
117_H 256_A 1.344 0.77
59_L 85_V 1.336 0.77
217_S 272_N 1.334 0.77
299_T 345_D 1.328 0.76
210_P 231_T 1.313 0.75
147_E 176_T 1.312 0.75
36_G 75_R 1.304 0.74
21_L 68_L 1.302 0.74
54_D 293_Q 1.3 0.74
206_V 224_P 1.298 0.74
39_K 52_E 1.297 0.74
109_L 114_S 1.293 0.73
80_I 283_L 1.287 0.73
10_L 35_V 1.286 0.73
108_F 208_S 1.28 0.72
156_V 169_V 1.263 0.71
19_E 62_L 1.259 0.70
19_E 58_R 1.256 0.70
140_A 175_H 1.247 0.69
210_P 230_Y 1.246 0.69
50_D 54_D 1.244 0.69
123_Y 282_V 1.24 0.69
190_H 212_P 1.235 0.68
55_L 59_L 1.233 0.68
207_Y 249_V 1.232 0.68
346_I 355_M 1.229 0.68
190_H 211_Q 1.229 0.68
163_S 166_I 1.229 0.68
149_G 285_R 1.218 0.67
124_A 348_A 1.217 0.67
16_A 20_K 1.213 0.66
329_E 345_D 1.202 0.65
26_A 66_H 1.197 0.65
113_G 117_H 1.191 0.64
17_L 24_L 1.19 0.64
232_E 236_A 1.189 0.64
206_V 223_Y 1.183 0.63
22_L 62_L 1.178 0.63
173_L 219_F 1.178 0.63
131_F 151_A 1.175 0.63
39_K 51_R 1.175 0.63
114_S 299_T 1.171 0.62
168_D 209_H 1.17 0.62
188_I 261_Y 1.168 0.62
120_A 222_R 1.168 0.62
115_Y 191_C 1.163 0.62
26_A 63_G 1.162 0.61
348_A 355_M 1.158 0.61
144_N 274_R 1.154 0.61
25_L 206_V 1.15 0.60
10_L 38_A 1.145 0.60
23_A 360_K 1.144 0.60
47_R 85_V 1.139 0.59
180_I 372_L 1.137 0.59
318_L 358_A 1.134 0.59
298_T 374_C 1.125 0.58
308_A 325_M 1.12 0.57
206_V 226_W 1.119 0.57
140_A 168_D 1.118 0.57
360_K 364_E 1.118 0.57
124_A 153_Y 1.117 0.57
118_L 254_S 1.11 0.56
13_K 16_A 1.109 0.56
33_V 37_K 1.108 0.56
32_A 182_G 1.105 0.56
234_T 324_I 1.105 0.56
147_E 177_S 1.104 0.56
106_I 356_Q 1.101 0.55
12_E 310_A 1.099 0.55
36_G 46_V 1.095 0.55
313_E 316_L 1.09 0.54
312_V 316_L 1.082 0.53
176_T 370_K 1.078 0.53
116_S 339_E 1.076 0.53
68_L 76_L 1.072 0.52
7_L 11_R 1.069 0.52
53_R 57_E 1.068 0.52
217_S 311_L 1.067 0.52
178_L 325_M 1.067 0.52
205_T 229_E 1.065 0.52
213_F 230_Y 1.065 0.52
123_Y 338_W 1.064 0.51
221_N 228_I 1.063 0.51
105_R 151_A 1.058 0.51
213_F 228_I 1.056 0.51
287_A 350_L 1.053 0.50
171_D 332_P 1.052 0.50
18_D 28_R 1.05 0.50
29_R 82_E 1.044 0.49
60_I 74_T 1.043 0.49
345_D 358_A 1.043 0.49
311_L 324_I 1.038 0.49
223_Y 330_S 1.035 0.48
82_E 85_V 1.033 0.48
197_T 266_L 1.032 0.48
84_S 88_Q 1.032 0.48
11_R 52_E 1.028 0.48
84_S 182_G 1.025 0.47
262_G 371_V 1.024 0.47
12_E 16_A 1.019 0.47
25_L 80_I 1.019 0.47
313_E 319_R 1.014 0.46
58_R 62_L 1.014 0.46
204_N 254_S 1.012 0.46
238_M 263_L 1.011 0.46
141_D 222_R 1.008 0.46
212_P 254_S 1.007 0.46
216_C 322_N 1.007 0.46
303_A 370_K 1.004 0.45
329_E 332_P 1.002 0.45
314_A 366_T 1.001 0.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2qmxA20.7151000.315Contact Map0.607
3mwbA20.74091000.318Contact Map0.557
3luyA10.72541000.345Contact Map0.494
2qmwA20.68391000.346Contact Map0.542
1phzA10.31611000.797Contact Map0.358
3nvtA20.810999.90.822Contact Map0.474
1ecmA20.282499.90.822Contact Map0.662
2gtvX10.248799.90.846Contact Map0.443
3retA20.253999.90.847Contact Map0.622
3rmiA10.277299.90.847Contact Map0.328
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.2487 seconds.