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OPENSEQ.org
HIS2 - Histidine biosynthesis bifunctional protein HisIE
UniProt: P06989 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10451
Length: 203 (198)
Sequences: 770
Seq/Len: 3.89
HIS2
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
45_T 74_V 4.113 1.00
135_T 178_Y 3.719 1.00
10_L 19_M 2.947 1.00
60_R 66_E 2.799 1.00
22_I 34_L 2.761 1.00
171_N 197_N 2.747 1.00
51_V 88_V 2.465 1.00
144_K 163_V 2.447 1.00
44_K 47_E 2.389 1.00
124_E 128_A 2.221 1.00
92_P 95_P 2.193 1.00
13_E 16_D 2.172 1.00
50_K 63_T 2.143 1.00
54_F 59_Q 2.14 1.00
35_G 96_T 2.098 1.00
52_T 63_T 2.067 1.00
21_V 88_V 2.055 1.00
197_N 200_K 2.023 1.00
10_L 79_P 1.965 1.00
71_F 93_I 1.938 1.00
148_Q 155_V 1.913 1.00
73_N 93_I 1.907 1.00
23_V 53_F 1.906 1.00
144_K 162_T 1.896 1.00
75_V 91_N 1.838 1.00
157_T 173_A 1.809 0.99
170_T 197_N 1.79 0.99
119_E 185_Q 1.781 0.99
39_P 43_D 1.721 0.99
11_D 14_K 1.705 0.99
50_K 73_N 1.633 0.99
155_V 158_A 1.627 0.99
50_K 71_F 1.616 0.99
44_K 48_S 1.591 0.98
21_V 41_A 1.588 0.98
34_L 85_T 1.587 0.98
37_M 88_V 1.586 0.98
130_P 136_A 1.569 0.98
129_D 136_A 1.554 0.98
30_E 102_S 1.537 0.98
43_D 47_E 1.526 0.98
21_V 37_M 1.508 0.98
23_V 90_A 1.504 0.97
73_N 91_N 1.458 0.97
151_G 155_V 1.457 0.97
142_G 146_I 1.428 0.96
44_K 61_L 1.423 0.96
53_F 92_P 1.414 0.96
51_V 74_V 1.413 0.96
173_A 184_L 1.386 0.95
53_F 72_L 1.362 0.95
101_T 106_G 1.352 0.94
60_R 67_T 1.332 0.94
53_F 88_V 1.326 0.94
123_A 185_Q 1.32 0.94
154_G 158_A 1.317 0.93
72_L 96_T 1.314 0.93
8_R 39_P 1.281 0.92
77_I 88_V 1.276 0.92
33_M 87_L 1.267 0.92
143_T 186_D 1.257 0.91
74_V 105_F 1.252 0.91
178_Y 182_V 1.246 0.91
135_T 182_V 1.227 0.90
122_L 139_Y 1.222 0.90
174_S 197_N 1.201 0.88
126_K 185_Q 1.188 0.88
174_S 194_V 1.173 0.87
173_A 177_M 1.167 0.86
72_L 90_A 1.165 0.86
126_K 139_Y 1.165 0.86
15_T 57_T 1.148 0.85
161_A 183_L 1.144 0.85
35_G 51_V 1.143 0.85
72_L 92_P 1.142 0.85
70_N 95_P 1.132 0.84
173_A 180_L 1.13 0.84
23_V 88_V 1.125 0.84
160_A 168_E 1.12 0.83
115_L 191_L 1.114 0.83
169_L 189_L 1.101 0.82
99_K 106_G 1.089 0.81
25_H 102_S 1.075 0.80
63_T 66_E 1.071 0.79
132_T 139_Y 1.069 0.79
190_D 193_T 1.068 0.79
151_G 158_A 1.068 0.79
26_A 75_V 1.055 0.78
70_N 93_I 1.053 0.78
50_K 66_E 1.05 0.77
121_L 202_H 1.048 0.77
122_L 181_L 1.047 0.77
76_S 89_L 1.043 0.77
51_V 72_L 1.041 0.76
74_V 88_V 1.039 0.76
145_R 151_G 1.037 0.76
78_A 89_L 1.033 0.76
145_R 159_L 1.029 0.75
136_A 140_A 1.018 0.74
119_E 191_L 1.015 0.74
44_K 52_T 1.015 0.74
161_A 169_L 1.01 0.73
157_T 180_L 1.005 0.73
181_L 191_L 1.004 0.73
53_F 90_A 1.003 0.73
129_D 132_T 1.001 0.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1zpsA20.65021000.429Contact Map0.758
2a7wA120.46311000.622Contact Map0.747
1yvwA40.45811000.631Contact Map0.84
1y6xA10.45811000.637Contact Map0.476
1yxbA80.46311000.64Contact Map0.664
2yxhA20.413899.10.854Contact Map0.436
2gtaA40.428698.70.873Contact Map0.688
1vmgA10.463197.90.899Contact Map0.317
2oieA40.46897.70.903Contact Map0.586
2q5zA20.492697.50.906Contact Map0.537
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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