May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
SYGB - Glycine--tRNA ligase beta subunit
UniProt: P00961 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10410
Length: 689 (685)
Sequences: 997
Seq/Len: 1.46
SYGB
Paralog alert: 0.00 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
388_R 422_E 4.827 1.00
210_R 221_E 4.568 1.00
350_Q 362_R 4.426 1.00
418_R 424_E 3.782 1.00
385_H 423_A 3.76 1.00
43_G 60_N 3.406 1.00
347_P 350_Q 3.162 1.00
40_L 127_T 3.138 1.00
369_Q 387_T 2.845 1.00
610_E 654_K 2.704 1.00
125_E 129_A 2.619 1.00
633_R 636_D 2.614 1.00
176_T 181_Q 2.588 1.00
444_P 490_I 2.58 1.00
341_R 344_D 2.576 1.00
230_A 302_I 2.553 1.00
6_F 166_L 2.551 1.00
187_R 196_E 2.509 1.00
128_E 171_K 2.491 1.00
651_F 671_M 2.422 1.00
82_D 86_K 2.391 1.00
67_D 123_K 2.388 1.00
91_A 95_A 2.387 1.00
622_R 674_K 2.369 1.00
609_P 612_I 2.354 0.99
362_R 366_D 2.345 0.99
31_F 56_L 2.3 0.99
229_D 260_V 2.286 0.99
414_M 418_R 2.275 0.99
389_A 429_A 2.263 0.99
251_V 288_F 2.252 0.99
499_D 555_T 2.21 0.99
48_F 206_P 2.181 0.99
433_Q 452_L 2.161 0.99
110_T 113_G 2.14 0.99
331_E 335_N 2.136 0.99
268_K 331_E 2.114 0.99
335_N 338_R 2.097 0.99
184_R 198_T 2.059 0.98
167_L 184_R 2.04 0.98
432_E 449_A 2.013 0.98
244_S 282_K 1.99 0.98
438_F 441_D 1.99 0.98
414_M 431_N 1.981 0.98
280_T 284_D 1.979 0.98
93_G 96_R 1.955 0.97
89_K 92_E 1.949 0.97
418_R 427_A 1.945 0.97
345_N 399_T 1.914 0.97
659_V 664_L 1.913 0.97
373_G 387_T 1.899 0.97
607_K 659_V 1.888 0.97
29_A 33_A 1.851 0.96
227_K 231_E 1.845 0.96
350_Q 366_D 1.844 0.96
338_R 419_H 1.834 0.96
64_A 67_D 1.833 0.96
385_H 425_D 1.822 0.96
174_P 181_Q 1.819 0.96
662_K 666_I 1.812 0.96
292_A 296_K 1.802 0.95
46_Q 218_D 1.797 0.95
95_A 100_I 1.77 0.95
446_N 449_A 1.761 0.95
72_K 97_G 1.758 0.95
239_G 290_V 1.751 0.94
71_E 119_R 1.739 0.94
92_E 96_R 1.728 0.94
200_D 204_Q 1.725 0.94
8_V 27_F 1.714 0.93
76_A 97_G 1.709 0.93
201_N 204_Q 1.703 0.93
224_A 228_A 1.701 0.93
431_N 435_Q 1.693 0.93
615_A 619_V 1.69 0.93
223_K 249_E 1.684 0.93
432_E 435_Q 1.682 0.93
430_L 433_Q 1.678 0.93
450_C 494_K 1.674 0.92
36_D 42_H 1.672 0.92
603_A 606_L 1.655 0.92
388_R 426_V 1.647 0.92
338_R 420_D 1.646 0.92
428_V 432_E 1.642 0.91
274_A 291_Y 1.629 0.91
352_V 479_A 1.616 0.91
19_A 145_P 1.612 0.90
67_D 124_G 1.605 0.90
606_L 612_I 1.595 0.90
265_F 270_L 1.595 0.90
184_R 199_I 1.593 0.90
393_S 433_Q 1.592 0.90
232_E 236_K 1.575 0.89
87_P 91_A 1.572 0.89
69_E 121_H 1.566 0.89
261_L 303_F 1.561 0.88
605_T 667_N 1.56 0.88
86_K 92_E 1.556 0.88
457_K 486_G 1.544 0.88
100_I 118_Y 1.536 0.87
216_I 225_K 1.536 0.87
207_E 210_R 1.53 0.87
536_Y 561_D 1.528 0.87
437_R 441_D 1.515 0.86
264_K 300_N 1.515 0.86
453_A 457_K 1.511 0.86
650_A 654_K 1.51 0.86
613_K 650_A 1.509 0.86
92_E 101_T 1.501 0.86
453_A 486_G 1.498 0.86
8_V 175_A 1.496 0.85
422_E 426_V 1.496 0.85
375_I 503_L 1.492 0.85
545_T 564_M 1.488 0.85
8_V 139_L 1.487 0.85
219_Y 253_S 1.485 0.85
392_L 422_E 1.477 0.84
276_A 401_M 1.474 0.84
199_I 208_I 1.473 0.84
454_I 487_V 1.472 0.84
126_S 129_A 1.469 0.84
132_P 136_A 1.469 0.84
69_E 119_R 1.458 0.83
77_I 107_R 1.455 0.83
89_K 96_R 1.455 0.83
504_T 524_V 1.441 0.82
10_I 161_V 1.44 0.82
645_R 649_D 1.439 0.82
559_D 634_Y 1.439 0.82
204_Q 208_I 1.437 0.82
6_F 61_L 1.437 0.82
89_K 97_G 1.433 0.82
206_P 217_A 1.425 0.81
403_F 406_T 1.419 0.81
11_G 163_T 1.412 0.81
266_E 324_R 1.407 0.80
125_E 133_N 1.404 0.80
488_L 557_P 1.403 0.80
364_K 460_T 1.403 0.80
461_L 524_V 1.4 0.80
609_P 613_K 1.395 0.79
12_T 157_F 1.393 0.79
10_I 164_V 1.382 0.79
227_K 245_E 1.379 0.78
361_L 364_K 1.378 0.78
40_L 61_L 1.367 0.78
98_C 120_A 1.362 0.77
72_K 98_C 1.358 0.77
260_V 304_V 1.355 0.77
541_Y 564_M 1.351 0.76
424_E 428_V 1.35 0.76
91_A 116_L 1.348 0.76
95_A 105_A 1.345 0.76
269_F 328_A 1.342 0.76
127_T 168_L 1.341 0.76
610_E 650_A 1.34 0.76
559_D 563_R 1.339 0.75
569_H 635_Q 1.337 0.75
658_M 669_L 1.333 0.75
666_I 670_T 1.332 0.75
347_P 362_R 1.329 0.75
197_F 213_G 1.328 0.75
662_K 665_R 1.325 0.74
74_G 94_W 1.325 0.74
228_A 232_E 1.317 0.74
24_A 49_A 1.316 0.74
342_L 392_L 1.316 0.74
378_Q 502_T 1.311 0.73
363_D 366_D 1.31 0.73
39_G 68_R 1.309 0.73
81_F 87_P 1.306 0.73
290_V 302_I 1.303 0.73
46_Q 59_A 1.3 0.72
216_I 257_W 1.3 0.72
386_A 452_L 1.299 0.72
55_A 165_T 1.292 0.72
491_I 496_L 1.291 0.72
231_E 235_R 1.29 0.72
224_A 227_K 1.288 0.71
358_L 463_G 1.273 0.70
372_A 452_L 1.272 0.70
497_N 556_R 1.272 0.70
33_A 37_N 1.271 0.70
489_R 493_E 1.264 0.69
261_L 316_I 1.257 0.69
28_A 32_T 1.252 0.68
428_V 446_N 1.25 0.68
275_E 415_H 1.246 0.68
369_Q 391_L 1.244 0.68
237_I 262_T 1.239 0.67
660_D 665_R 1.237 0.67
261_L 304_V 1.235 0.67
533_R 547_Q 1.222 0.66
388_R 420_D 1.22 0.65
345_N 420_D 1.219 0.65
237_I 300_N 1.214 0.65
77_I 114_E 1.213 0.65
277_L 281_M 1.213 0.65
230_A 260_V 1.212 0.65
340_K 348_R 1.21 0.64
75_P 94_W 1.21 0.64
260_V 302_I 1.204 0.64
342_L 395_C 1.202 0.64
166_L 175_A 1.195 0.63
63_E 168_L 1.186 0.62
71_E 108_L 1.186 0.62
445_S 449_A 1.18 0.62
450_C 490_I 1.179 0.61
292_A 295_G 1.176 0.61
55_A 217_A 1.173 0.61
457_K 479_A 1.171 0.61
91_A 102_V 1.162 0.60
173_I 182_S 1.161 0.60
492_V 535_W 1.159 0.59
172_V 184_R 1.158 0.59
487_V 528_M 1.154 0.59
432_E 446_N 1.153 0.59
605_T 661_D 1.151 0.59
8_V 164_V 1.146 0.58
109_T 112_K 1.145 0.58
214_K 308_E 1.143 0.58
651_F 657_V 1.142 0.58
30_N 142_L 1.14 0.58
68_R 122_V 1.14 0.58
618_V 675_L 1.138 0.57
214_K 257_W 1.136 0.57
226_I 248_L 1.127 0.56
353_L 360_T 1.123 0.56
346_L 395_C 1.123 0.56
409_Q 434_Y 1.122 0.56
281_M 287_Y 1.121 0.56
165_T 183_D 1.119 0.55
193_G 213_G 1.118 0.55
379_I 451_A 1.116 0.55
26_S 143_P 1.113 0.55
162_H 180_I 1.113 0.55
21_R 249_E 1.11 0.55
248_L 432_E 1.104 0.54
6_F 135_V 1.1 0.54
91_A 105_A 1.099 0.53
368_I 390_G 1.098 0.53
210_R 216_I 1.095 0.53
73_R 115_W 1.094 0.53
172_V 183_D 1.092 0.53
368_I 456_D 1.089 0.52
206_P 221_E 1.087 0.52
234_A 239_G 1.086 0.52
25_E 29_A 1.084 0.52
64_A 126_S 1.082 0.52
547_Q 687_L 1.079 0.51
72_K 120_A 1.079 0.51
108_L 117_L 1.079 0.51
287_Y 319_N 1.077 0.51
267_E 299_P 1.074 0.51
280_T 283_G 1.074 0.51
334_F 651_F 1.074 0.51
21_R 25_E 1.07 0.50
177_I 601_V 1.068 0.50
174_P 182_S 1.068 0.50
280_T 289_P 1.067 0.50
173_I 198_T 1.067 0.50
395_C 452_L 1.066 0.50
82_D 89_K 1.064 0.50
84_E 88_S 1.063 0.50
82_D 97_G 1.061 0.50
165_T 199_I 1.06 0.49
340_K 344_D 1.06 0.49
603_A 616_M 1.055 0.49
194_E 213_G 1.054 0.49
606_L 664_L 1.054 0.49
458_M 504_T 1.052 0.49
6_F 131_L 1.052 0.49
186_I 197_F 1.05 0.48
20_L 253_S 1.049 0.48
7_L 209_L 1.047 0.48
57_K 206_P 1.045 0.48
431_N 437_R 1.045 0.48
74_G 93_G 1.039 0.47
32_T 45_V 1.034 0.47
65_Q 130_L 1.034 0.47
15_L 576_A 1.032 0.47
544_D 571_R 1.031 0.46
602_N 605_T 1.03 0.46
432_E 445_S 1.029 0.46
444_P 457_K 1.029 0.46
505_E 521_V 1.028 0.46
616_M 620_V 1.026 0.46
495_N 562_A 1.024 0.46
436_P 444_P 1.021 0.45
345_N 398_M 1.021 0.45
364_K 459_D 1.021 0.45
342_L 420_D 1.018 0.45
425_D 446_N 1.018 0.45
175_A 182_S 1.018 0.45
367_R 459_D 1.015 0.45
187_R 197_F 1.013 0.45
239_G 299_P 1.012 0.45
42_H 58_V 1.011 0.44
642_A 645_R 1.01 0.44
364_K 368_I 1.008 0.44
355_Q 364_K 1.008 0.44
624_K 640_E 1.006 0.44
328_A 335_N 1.004 0.44
39_G 122_V 1.003 0.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ccgA10.243896.30.973Contact Map0.709
2ogiA20.25496.10.973Contact Map0.645
2o08A20.245395.70.974Contact Map0.739
2pq7A10.2787930.977Contact Map0.624
3dtoA40.294692.40.978Contact Map0.489
3b57A10.284589.20.979Contact Map0.587
3gw7A20.313588.70.98Contact Map0.432
2qgsA20.296188.50.98Contact Map0.599
3djbA20.294686.80.98Contact Map0.462
2pjqA40.3062860.981Contact Map0.55
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.3348 seconds.