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OPENSEQ.org
PPB - Alkaline phosphatase
UniProt: P00634 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10727
Length: 471 (429)
Sequences: 811
Seq/Len: 1.89
PPB
Paralog alert: 0.16 [within 20: 0.06] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
245_K 250_Q 4.528 1.00
164_A 335_N 3.511 1.00
261_D 264_S 3.216 1.00
347_S 350_K 3.149 1.00
110_A 126_A 3.05 1.00
246_T 249_E 3.021 1.00
367_L 371_V 2.966 1.00
171_A 346_A 2.911 1.00
174_Q 191_Y 2.8 1.00
262_A 266_N 2.76 1.00
177_T 342_Q 2.759 1.00
262_A 323_Q 2.73 1.00
372_Q 376_E 2.61 1.00
122_T 127_S 2.601 1.00
326_D 373_R 2.586 1.00
226_L 277_L 2.56 1.00
287_P 318_V 2.485 1.00
159_K 223_D 2.422 1.00
366_D 369_E 2.242 1.00
128_A 180_A 2.239 1.00
157_M 461_F 2.167 0.99
322_A 369_E 2.162 0.99
102_L 448_A 2.151 0.99
322_A 366_D 2.146 0.99
327_K 330_E 2.146 0.99
106_Y 441_I 2.095 0.99
268_V 277_L 2.089 0.99
169_S 346_A 2.05 0.99
72_G 348_I 2.022 0.99
137_T 141_A 1.999 0.99
387_I 451_V 1.982 0.99
173_L 225_T 1.931 0.98
215_E 219_N 1.91 0.98
230_A 258_L 1.894 0.98
104_G 452_V 1.886 0.98
67_I 468_L 1.879 0.98
152_P 160_A 1.867 0.98
322_A 373_R 1.865 0.98
248_R 258_L 1.864 0.98
141_A 144_V 1.853 0.98
393_A 437_S 1.848 0.98
83_A 364_T 1.842 0.98
326_D 330_E 1.836 0.98
80_I 442_A 1.829 0.98
163_L 337_K 1.828 0.98
68_I 374_A 1.806 0.97
154_I 464_M 1.804 0.97
328_A 341_L 1.804 0.97
191_Y 285_N 1.782 0.97
97_I 368_D 1.761 0.97
327_K 331_L 1.756 0.97
70_L 386_V 1.748 0.96
182_V 217_L 1.695 0.96
174_Q 214_T 1.694 0.96
76_G 390_A 1.673 0.95
133_T 143_G 1.662 0.95
155_L 165_T 1.649 0.95
226_L 279_G 1.645 0.95
154_I 461_F 1.626 0.94
153_T 156_E 1.615 0.94
71_I 131_W 1.611 0.94
167_N 181_L 1.582 0.93
370_A 374_A 1.582 0.93
155_L 340_F 1.575 0.93
131_W 342_Q 1.567 0.93
172_E 191_Y 1.565 0.92
345_G 367_L 1.564 0.92
226_L 268_V 1.534 0.91
289_R 320_T 1.522 0.91
329_I 374_A 1.518 0.91
106_Y 460_L 1.511 0.91
330_E 377_F 1.502 0.90
159_K 165_T 1.497 0.90
83_A 365_V 1.486 0.90
104_G 441_I 1.482 0.90
143_G 151_H 1.478 0.89
356_N 359_G 1.469 0.89
175_D 350_K 1.465 0.89
72_G 75_M 1.462 0.89
76_G 393_A 1.448 0.88
142_L 180_A 1.443 0.88
192_G 232_T 1.433 0.87
363_E 366_D 1.429 0.87
86_Y 365_V 1.427 0.87
110_A 122_T 1.421 0.87
282_A 287_P 1.405 0.86
75_M 348_I 1.403 0.86
122_T 139_N 1.397 0.85
104_G 455_T 1.39 0.85
354_A 431_S 1.387 0.85
77_D 440_R 1.368 0.84
226_L 265_L 1.359 0.83
171_A 350_K 1.358 0.83
459_D 463_T 1.353 0.83
289_R 366_D 1.344 0.82
451_V 463_T 1.339 0.82
377_F 381_E 1.33 0.81
142_L 179_A 1.326 0.81
156_E 184_H 1.318 0.81
258_L 280_L 1.314 0.80
126_A 139_N 1.312 0.80
97_I 442_A 1.306 0.80
256_Y 276_P 1.293 0.79
268_V 327_K 1.286 0.78
379_K 383_N 1.275 0.77
426_N 432_Q 1.275 0.77
163_L 468_L 1.271 0.77
138_Y 141_A 1.267 0.77
169_S 173_L 1.264 0.77
76_G 437_S 1.255 0.76
84_R 438_Q 1.252 0.76
70_L 371_V 1.238 0.75
128_A 132_S 1.236 0.74
166_G 341_L 1.229 0.74
173_L 280_L 1.228 0.74
79_E 352_D 1.228 0.74
122_T 126_A 1.217 0.73
72_G 389_T 1.216 0.73
67_I 464_M 1.214 0.72
68_I 378_A 1.212 0.72
76_G 396_S 1.21 0.72
215_E 245_K 1.209 0.72
375_L 444_Y 1.205 0.72
226_L 324_M 1.204 0.72
288_V 347_S 1.197 0.71
373_R 376_E 1.196 0.71
279_G 324_M 1.192 0.70
393_A 435_T 1.191 0.70
75_M 80_I 1.188 0.70
325_T 374_A 1.188 0.70
348_I 364_T 1.173 0.69
143_G 153_T 1.171 0.69
270_E 327_K 1.17 0.68
110_A 139_N 1.165 0.68
325_T 343_V 1.164 0.68
174_Q 285_N 1.161 0.68
329_I 377_F 1.157 0.67
461_F 465_K 1.151 0.67
71_I 389_T 1.15 0.66
266_N 327_K 1.146 0.66
69_L 340_F 1.144 0.66
321_L 366_D 1.139 0.65
131_W 460_L 1.134 0.65
259_V 277_L 1.129 0.64
285_N 350_K 1.127 0.64
234_A 246_T 1.125 0.64
84_R 98_D 1.125 0.64
378_A 444_Y 1.119 0.63
346_A 350_K 1.115 0.63
71_I 177_T 1.11 0.62
70_L 345_G 1.11 0.62
277_L 331_L 1.104 0.62
268_V 367_L 1.103 0.62
260_S 282_A 1.103 0.62
279_G 319_P 1.09 0.60
441_I 460_L 1.086 0.60
142_L 183_A 1.084 0.60
281_F 319_P 1.082 0.60
158_A 165_T 1.079 0.59
345_G 370_A 1.079 0.59
140_G 187_S 1.076 0.59
76_G 110_A 1.072 0.58
78_S 437_S 1.07 0.58
340_F 364_T 1.061 0.57
375_L 386_V 1.06 0.57
87_A 368_D 1.049 0.56
157_M 465_K 1.043 0.55
386_V 444_Y 1.041 0.55
100_L 444_Y 1.041 0.55
352_D 424_Y 1.04 0.55
53_A 56_R 1.037 0.55
127_S 457_Q 1.035 0.54
329_I 378_A 1.033 0.54
143_G 180_A 1.029 0.54
106_Y 387_I 1.028 0.54
183_A 213_I 1.023 0.53
151_H 184_H 1.02 0.53
33_A 52_T 1.019 0.53
450_N 466_A 1.018 0.53
363_E 430_D 1.015 0.52
172_E 285_N 1.013 0.52
265_L 279_G 1.012 0.52
378_A 386_V 1.01 0.52
68_I 279_G 1.008 0.52
83_A 113_K 1.007 0.51
275_K 334_K 1.001 0.51
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3tg0A40.95331000.183Contact Map0.737
1k7hA20.8451000.242Contact Map0.773
3e2dA20.77071000.269Contact Map0.73
1zedA10.85991000.285Contact Map0.794
2w0yA20.83011000.31Contact Map0.626
3a52A20.73251000.493Contact Map0.733
2w5vA20.78561000.67Contact Map0.69
3q3qA10.798399.50.907Contact Map0.481
2gsoA20.615799.40.909Contact Map0.575
2zktA20.664599.40.911Contact Map0.609
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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