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OPENSEQ.org
DHE4 - NADP-specific glutamate dehydrogenase
UniProt: P00370 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10372
Length: 447 (438)
Sequences: 1929
Seq/Len: 4.40
DHE4
Paralog alert: 0.27 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
251_A 256_A 4.63 1.00
236_S 315_V 3.672 1.00
58_E 86_S 3.262 1.00
336_I 358_L 3.069 1.00
237_V 248_I 3.044 1.00
284_E 289_R 2.709 1.00
141_G 145_R 2.688 1.00
261_A 282_L 2.672 1.00
357_E 361_Q 2.67 1.00
124_M 381_G 2.551 1.00
248_I 261_A 2.549 1.00
315_V 340_V 2.498 1.00
66_V 149_A 2.46 1.00
335_L 340_V 2.454 1.00
312_S 334_Q 2.414 1.00
134_D 137_G 2.357 1.00
234_R 257_R 2.298 1.00
221_A 224_K 2.283 1.00
84_F 110_G 2.262 1.00
360_Q 424_N 2.212 1.00
62_Q 103_L 2.198 1.00
219_T 344_A 2.177 1.00
206_I 395_A 2.165 1.00
228_M 233_M 2.155 1.00
15_Q 29_R 2.146 1.00
278_K 281_R 2.08 1.00
152_T 182_M 2.077 1.00
221_A 407_L 2.058 1.00
172_G 175_E 2.037 1.00
271_E 303_V 2.021 1.00
259_I 269_V 1.997 1.00
249_E 253_E 1.981 1.00
133_F 138_K 1.979 1.00
235_V 251_A 1.968 1.00
70_D 139_S 1.966 1.00
221_A 411_H 1.94 1.00
397_K 401_R 1.934 1.00
225_R 410_H 1.931 1.00
235_V 256_A 1.922 1.00
351_T 359_F 1.902 1.00
95_M 179_M 1.892 1.00
264_S 286_K 1.889 1.00
281_R 300_F 1.879 1.00
328_D 331_A 1.871 1.00
329_V 354_E 1.85 1.00
49_S 73_Q 1.826 1.00
220_E 403_H 1.825 1.00
69_D 75_Q 1.819 1.00
220_E 224_K 1.801 1.00
225_R 414_V 1.783 1.00
182_M 185_K 1.775 1.00
155_Y 187_S 1.766 1.00
215_L 346_G 1.765 1.00
151_M 179_M 1.755 1.00
82_V 110_G 1.746 0.99
419_E 435_V 1.706 0.99
415_E 419_E 1.702 0.99
79_A 150_L 1.689 0.99
332_A 358_L 1.689 0.99
265_S 304_Y 1.688 0.99
251_A 258_V 1.684 0.99
260_T 310_P 1.683 0.99
97_F 171_V 1.667 0.99
169_I 323_T 1.65 0.99
284_E 288_S 1.647 0.99
260_T 313_L 1.632 0.99
18_D 21_Q 1.631 0.99
247_A 321_C 1.611 0.99
314_P 339_G 1.595 0.99
45_Y 439_D 1.592 0.99
236_S 260_T 1.591 0.99
230_F 256_A 1.59 0.99
329_V 333_H 1.584 0.99
265_S 292_R 1.583 0.99
95_M 150_L 1.553 0.99
88_I 121_T 1.541 0.98
410_H 425_Y 1.539 0.98
235_V 317_I 1.535 0.98
327_L 335_L 1.519 0.98
333_H 337_A 1.518 0.98
215_L 344_A 1.518 0.98
149_A 185_K 1.513 0.98
8_E 12_N 1.497 0.98
176_V 201_F 1.491 0.98
235_V 319_L 1.486 0.98
289_R 299_E 1.478 0.98
247_A 319_L 1.473 0.98
422_Q 427_Q 1.472 0.98
344_A 372_A 1.472 0.98
133_F 146_F 1.464 0.98
347_A 350_P 1.459 0.98
11_L 15_Q 1.457 0.97
330_D 333_H 1.439 0.97
270_D 296_Y 1.427 0.97
140_E 174_R 1.419 0.97
249_E 283_I 1.416 0.97
14_V 28_V 1.402 0.97
291_G 295_D 1.402 0.97
327_L 359_F 1.398 0.97
248_I 282_L 1.396 0.97
82_V 103_L 1.395 0.97
173_G 208_P 1.39 0.96
155_Y 186_L 1.387 0.96
333_H 358_L 1.386 0.96
410_H 414_V 1.382 0.96
68_V 446_V 1.378 0.96
228_M 341_K 1.356 0.96
336_I 362_A 1.355 0.96
435_V 439_D 1.353 0.96
138_K 146_F 1.348 0.96
152_T 185_K 1.345 0.95
424_N 427_Q 1.343 0.95
200_S 439_D 1.342 0.95
56_E 78_R 1.338 0.95
416_H 420_G 1.318 0.95
84_F 114_T 1.316 0.95
41_Q 419_E 1.311 0.95
13_H 16_K 1.311 0.95
243_V 346_G 1.307 0.94
100_S 105_I 1.305 0.94
329_V 358_L 1.293 0.94
259_I 315_V 1.29 0.94
267_T 305_L 1.285 0.94
61_I 157_H 1.283 0.94
225_R 418_G 1.279 0.94
9_S 13_H 1.278 0.94
245_Q 283_I 1.274 0.93
9_S 46_R 1.271 0.93
351_T 355_A 1.268 0.93
358_L 362_A 1.268 0.93
60_V 107_K 1.266 0.93
267_T 307_G 1.266 0.93
217_Y 399_D 1.26 0.93
367_A 372_A 1.257 0.93
147_C 151_M 1.257 0.93
234_R 316_D 1.255 0.93
68_V 141_G 1.249 0.93
215_L 219_T 1.248 0.92
114_T 437_V 1.241 0.92
86_S 91_Y 1.24 0.92
63_F 154_L 1.22 0.91
11_L 29_R 1.215 0.91
281_R 296_Y 1.21 0.91
19_P 25_A 1.21 0.91
344_A 367_A 1.209 0.91
99_P 134_D 1.208 0.91
215_L 319_L 1.202 0.90
234_R 271_E 1.199 0.90
102_N 105_I 1.195 0.90
216_V 251_A 1.189 0.90
396_E 400_A 1.184 0.90
60_V 103_L 1.184 0.90
423_T 431_I 1.169 0.89
48_M 442_L 1.167 0.89
411_H 415_E 1.166 0.89
318_A 335_L 1.165 0.89
117_N 377_V 1.165 0.89
91_Y 127_G 1.162 0.88
245_Q 287_A 1.157 0.88
12_N 16_K 1.156 0.88
285_I 290_D 1.148 0.88
306_E 309_Q 1.145 0.87
120_T 374_A 1.143 0.87
283_I 287_A 1.141 0.87
248_I 279_L 1.14 0.87
311_W 318_A 1.138 0.87
177_G 198_G 1.135 0.87
236_S 259_I 1.134 0.87
403_H 407_L 1.122 0.86
439_D 442_L 1.121 0.86
215_L 321_C 1.114 0.85
356_T 426_V 1.102 0.85
327_L 366_F 1.095 0.84
404_H 408_D 1.094 0.84
12_N 15_Q 1.09 0.84
174_R 177_G 1.087 0.83
115_F 370_K 1.085 0.83
180_A 190_T 1.084 0.83
251_A 319_L 1.082 0.83
385_A 389_A 1.081 0.83
85_S 441_M 1.08 0.83
296_Y 302_L 1.078 0.83
235_V 258_V 1.076 0.83
336_I 364_V 1.069 0.82
394_K 397_K 1.067 0.82
15_Q 25_A 1.067 0.82
151_M 194_F 1.063 0.82
252_M 258_V 1.063 0.82
387_N 392_G 1.061 0.81
60_V 82_V 1.06 0.81
394_K 404_H 1.053 0.81
249_E 279_L 1.051 0.81
97_F 147_C 1.049 0.80
225_R 411_H 1.049 0.80
66_V 76_V 1.041 0.80
252_M 279_L 1.041 0.80
78_R 132_D 1.04 0.80
149_A 182_M 1.037 0.79
271_E 314_P 1.037 0.79
162_T 188_N 1.034 0.79
166_A 377_V 1.028 0.78
351_T 356_T 1.027 0.78
434_F 437_V 1.02 0.78
97_F 131_S 1.017 0.77
98_H 323_T 1.016 0.77
278_K 300_F 1.016 0.77
23_E 104_S 1.016 0.77
220_E 254_F 1.016 0.77
359_F 364_V 1.015 0.77
38_F 431_I 1.015 0.77
213_Y 250_K 1.013 0.77
65_V 149_A 1.012 0.77
312_S 331_A 1.008 0.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fccA120.99781000.111Contact Map0.776
1bgvA10.99331000.121Contact Map0.675
2bmaA60.99331000.128Contact Map0.774
3r3jA60.99331000.131Contact Map0.731
3mw9A60.94181000.164Contact Map0.758
2tmgA60.92171000.166Contact Map0.755
2yfqA20.92621000.168Contact Map0.694
3aoeE40.93291000.179Contact Map0.782
3aogA120.96421000.183Contact Map0.802
3k92A60.94631000.187Contact Map0.783
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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