| keto_e69_WreU |
| ID: | 1706845099 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 188 (188) | ||
| Sequences: | 846 (472.4) | ||
| Seq/Len: | 4.500 | ||
| Nf(neff/√len): | 34.5 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 159_E | 163_R | 3.967 | 1.00 |
| 139_W | 143_N | 3.617 | 1.00 |
| 100_I | 136_L | 3.615 | 1.00 |
| 165_S | 168_F | 3.541 | 1.00 |
| 142_I | 175_L | 2.770 | 1.00 |
| 16_S | 98_W | 2.682 | 1.00 |
| 9_F | 101_L | 2.520 | 1.00 |
| 45_I | 51_I | 2.492 | 1.00 |
| 159_E | 168_F | 2.417 | 1.00 |
| 118_Y | 122_E | 2.320 | 1.00 |
| 163_R | 168_F | 2.285 | 1.00 |
| 11_L | 107_F | 2.246 | 1.00 |
| 49_N | 125_T | 2.178 | 1.00 |
| 166_L | 170_M | 2.096 | 1.00 |
| 108_V | 173_L | 2.080 | 1.00 |
| 33_T | 81_P | 1.948 | 1.00 |
| 9_F | 12_A | 1.891 | 1.00 |
| 43_K | 51_I | 1.846 | 1.00 |
| 55_P | 99_C | 1.817 | 1.00 |
| 29_S | 82_I | 1.775 | 1.00 |
| 74_N | 77_R | 1.762 | 1.00 |
| 41_W | 55_P | 1.752 | 1.00 |
| 98_W | 102_A | 1.735 | 0.99 |
| 168_F | 172_I | 1.626 | 0.99 |
| 43_K | 53_L | 1.612 | 0.99 |
| 152_E | 156_F | 1.539 | 0.98 |
| 48_F | 131_K | 1.533 | 0.98 |
| 59_S | 86_L | 1.461 | 0.98 |
| 107_F | 173_L | 1.439 | 0.97 |
| 143_N | 168_F | 1.422 | 0.97 |
| 139_W | 168_F | 1.413 | 0.97 |
| 129_V | 158_V | 1.394 | 0.97 |
| 99_C | 104_K | 1.379 | 0.96 |
| 63_D | 166_L | 1.378 | 0.96 |
| 125_T | 130_D | 1.365 | 0.96 |
| 149_P | 152_E | 1.358 | 0.96 |
| 172_I | 187_K | 1.338 | 0.95 |
| 118_Y | 121_I | 1.324 | 0.95 |
| 54_M | 98_W | 1.320 | 0.95 |
| 127_Y | 158_V | 1.319 | 0.95 |
| 26_V | 30_V | 1.319 | 0.95 |
| 179_K | 184_K | 1.309 | 0.95 |
| 171_R | 174_F | 1.306 | 0.95 |
| 154_V | 158_V | 1.257 | 0.93 |
| 34_S | 59_S | 1.254 | 0.93 |
| 139_W | 156_F | 1.253 | 0.93 |
| 86_L | 91_L | 1.246 | 0.93 |
| 178_E | 181_V | 1.243 | 0.93 |
| 155_K | 159_E | 1.215 | 0.91 |
| 41_W | 99_C | 1.205 | 0.91 |
| 49_N | 130_D | 1.194 | 0.90 |
| 13_L | 17_V | 1.191 | 0.90 |
| 129_V | 161_L | 1.189 | 0.90 |
| 61_R | 78_F | 1.184 | 0.90 |
| 27_A | 38_I | 1.180 | 0.90 |
| 139_W | 172_I | 1.171 | 0.89 |
| 42_S | 188_H | 1.165 | 0.89 |
| 41_W | 54_M | 1.159 | 0.89 |
| 54_M | 99_C | 1.142 | 0.88 |
| 47_R | 50_Q | 1.139 | 0.87 |
| 40_Y | 66_T | 1.136 | 0.87 |
| 108_V | 177_A | 1.105 | 0.85 |
| 42_S | 54_M | 1.093 | 0.84 |
| 34_S | 54_M | 1.091 | 0.84 |
| 15_A | 107_F | 1.089 | 0.84 |
| 167_G | 170_M | 1.086 | 0.84 |
| 171_R | 175_L | 1.085 | 0.84 |
| 51_I | 118_Y | 1.078 | 0.83 |
| 98_W | 101_L | 1.077 | 0.83 |
| 132_L | 161_L | 1.070 | 0.83 |
| 20_L | 23_I | 1.067 | 0.82 |
| 113_A | 117_Q | 1.063 | 0.82 |
| 173_L | 177_A | 1.054 | 0.81 |
| 79_L | 84_S | 1.049 | 0.81 |
| 179_K | 183_R | 1.044 | 0.80 |
| 40_Y | 43_K | 1.040 | 0.80 |
| 5_R | 12_A | 1.040 | 0.80 |
| 16_S | 20_L | 1.037 | 0.80 |
| 59_S | 174_F | 1.032 | 0.79 |
| 76_E | 84_S | 1.029 | 0.79 |
| 26_V | 156_F | 1.024 | 0.79 |
| 34_S | 61_R | 1.019 | 0.78 |
| 73_E | 78_F | 1.011 | 0.77 |
| 118_Y | 151_P | 1.011 | 0.77 |
| 68_A | 125_T | 1.003 | 0.77 |
| 68_A | 115_Y | 1.002 | 0.77 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1vpyA | 1 | 0.234 | 32.9 | 0.965 | Contact Map |
| 3eojA | 1 | 0.3617 | 9.7 | 0.973 | Contact Map |
| 4oifA | 3 | 0.5053 | 8.8 | 0.974 | Contact Map |
| 3h4qA | 1 | 0.4734 | 6.9 | 0.975 | Contact Map |
| 2ccqA | 1 | 0.4574 | 6.2 | 0.975 | Contact Map |
| 3vxvA | 1 | 0.3085 | 5.8 | 0.976 | Contact Map |
| 2bjdA | 2 | 0.383 | 5.3 | 0.976 | Contact Map |
| 3vthA | 2 | 0.4521 | 4.6 | 0.977 | Contact Map |
| 3wwvA | 1 | 0.3032 | 4.4 | 0.977 | Contact Map |
| 1apsA | 1 | 0.4309 | 4.3 | 0.977 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)