| diNAcBac_cluster_v1 |
| ID: | 1699063530 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 201 (201) | ||
| Sequences: | 2469 (1789.9) | ||
| Seq/Len: | 12.284 | ||
| Nf(neff/√len): | 126.2 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 54_I | 116_V | 4.599 | 1.00 |
| 117_E | 196_K | 4.510 | 1.00 |
| 128_H | 162_K | 3.860 | 1.00 |
| 125_T | 155_E | 3.701 | 1.00 |
| 128_H | 131_D | 3.214 | 1.00 |
| 44_T | 56_K | 2.979 | 1.00 |
| 139_L | 143_N | 2.854 | 1.00 |
| 178_E | 182_K | 2.772 | 1.00 |
| 121_I | 196_K | 2.718 | 1.00 |
| 52_E | 127_K | 2.714 | 1.00 |
| 152_K | 155_E | 2.579 | 1.00 |
| 64_S | 73_L | 2.501 | 1.00 |
| 101_V | 136_I | 2.459 | 1.00 |
| 67_R | 73_L | 2.446 | 1.00 |
| 89_S | 183_R | 2.437 | 1.00 |
| 139_L | 159_Y | 2.409 | 1.00 |
| 46_A | 54_I | 2.325 | 1.00 |
| 29_T | 92_L | 2.270 | 1.00 |
| 121_I | 198_N | 2.224 | 1.00 |
| 159_Y | 163_N | 2.156 | 1.00 |
| 57_I | 97_Q | 2.083 | 1.00 |
| 188_K | 195_E | 2.039 | 1.00 |
| 68_D | 72_E | 2.024 | 1.00 |
| 151_E | 200_K | 2.019 | 1.00 |
| 34_Y | 39_R | 2.007 | 1.00 |
| 46_A | 56_K | 1.923 | 1.00 |
| 148_I | 153_K | 1.901 | 1.00 |
| 179_K | 184_S | 1.895 | 1.00 |
| 125_T | 162_K | 1.878 | 1.00 |
| 109_I | 137_T | 1.808 | 1.00 |
| 191_Q | 195_E | 1.798 | 1.00 |
| 8_R | 12_I | 1.795 | 1.00 |
| 12_I | 16_L | 1.738 | 1.00 |
| 80_L | 85_K | 1.738 | 1.00 |
| 19_L | 99_F | 1.720 | 1.00 |
| 19_L | 102_L | 1.677 | 1.00 |
| 171_K | 175_Q | 1.669 | 1.00 |
| 16_L | 102_L | 1.659 | 1.00 |
| 42_I | 58_Y | 1.582 | 1.00 |
| 188_K | 191_Q | 1.574 | 1.00 |
| 46_A | 116_V | 1.572 | 1.00 |
| 64_S | 67_R | 1.569 | 1.00 |
| 121_I | 199_G | 1.542 | 1.00 |
| 76_D | 193_T | 1.536 | 1.00 |
| 30_A | 41_V | 1.522 | 1.00 |
| 48_P | 115_L | 1.517 | 1.00 |
| 33_I | 37_V | 1.512 | 1.00 |
| 149_S | 153_K | 1.497 | 1.00 |
| 36_K | 83_F | 1.471 | 1.00 |
| 86_L | 90_L | 1.471 | 1.00 |
| 61_K | 65_D | 1.457 | 1.00 |
| 68_D | 71_G | 1.444 | 1.00 |
| 119_L | 130_H | 1.442 | 1.00 |
| 37_V | 61_K | 1.428 | 1.00 |
| 32_F | 36_K | 1.407 | 0.99 |
| 12_I | 102_L | 1.400 | 0.99 |
| 71_G | 74_L | 1.339 | 0.99 |
| 14_G | 108_F | 1.336 | 0.99 |
| 174_L | 178_E | 1.324 | 0.99 |
| 25_I | 29_T | 1.320 | 0.99 |
| 18_L | 22_T | 1.318 | 0.99 |
| 97_Q | 106_M | 1.308 | 0.99 |
| 19_L | 23_S | 1.290 | 0.99 |
| 44_T | 58_Y | 1.278 | 0.99 |
| 123_N | 200_K | 1.248 | 0.99 |
| 139_L | 175_Q | 1.243 | 0.98 |
| 8_R | 133_R | 1.233 | 0.98 |
| 34_Y | 37_V | 1.229 | 0.98 |
| 14_G | 18_L | 1.216 | 0.98 |
| 121_I | 126_Q | 1.197 | 0.98 |
| 101_V | 108_F | 1.192 | 0.98 |
| 37_V | 83_F | 1.186 | 0.98 |
| 82_K | 86_L | 1.186 | 0.98 |
| 198_N | 201_N | 1.168 | 0.97 |
| 64_S | 81_G | 1.165 | 0.97 |
| 142_V | 175_Q | 1.164 | 0.97 |
| 159_Y | 168_L | 1.158 | 0.97 |
| 187_S | 191_Q | 1.148 | 0.97 |
| 10_I | 14_G | 1.146 | 0.97 |
| 195_E | 198_N | 1.145 | 0.97 |
| 148_I | 156_Y | 1.141 | 0.97 |
| 173_A | 177_I | 1.128 | 0.97 |
| 33_I | 83_F | 1.124 | 0.97 |
| 175_Q | 179_K | 1.124 | 0.97 |
| 175_Q | 178_E | 1.123 | 0.97 |
| 187_S | 193_T | 1.123 | 0.97 |
| 152_K | 156_Y | 1.103 | 0.96 |
| 139_L | 156_Y | 1.101 | 0.96 |
| 109_I | 173_A | 1.098 | 0.96 |
| 48_P | 130_H | 1.079 | 0.95 |
| 108_F | 173_A | 1.078 | 0.95 |
| 33_I | 61_K | 1.077 | 0.95 |
| 4_N | 164_L | 1.075 | 0.95 |
| 126_Q | 199_G | 1.072 | 0.95 |
| 22_T | 26_I | 1.067 | 0.95 |
| 89_S | 97_Q | 1.063 | 0.95 |
| 12_I | 15_A | 1.050 | 0.94 |
| 30_A | 42_I | 1.039 | 0.94 |
| 58_Y | 99_F | 1.039 | 0.94 |
| 143_N | 175_Q | 1.034 | 0.94 |
| 68_D | 74_L | 1.028 | 0.94 |
| 8_R | 15_A | 1.027 | 0.94 |
| 26_I | 95_L | 1.025 | 0.93 |
| 188_K | 194_T | 1.015 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2m20A | 2 | 0.2985 | 6.6 | 0.975 | Contact Map |
| 1b0nB | 1 | 0.1443 | 2.7 | 0.979 | Contact Map |
| 4pj3A | 1 | 0.8955 | 2.3 | 0.98 | Contact Map |
| 4um9B | 1 | 0 | 2 | 0.98 | Contact Map |
| 4p55A | 2 | 0.4478 | 2 | 0.981 | Contact Map |
| 4we2A | 1 | 0.2537 | 1.9 | 0.981 | Contact Map |
| 1zfoA | 1 | 0.1493 | 1.9 | 0.981 | Contact Map |
| 1za0A | 1 | 0.398 | 1.9 | 0.981 | Contact Map |
| 4c48C | 1 | 0.2289 | 1.9 | 0.981 | Contact Map |
| 1r8uA | 1 | 0.2488 | 1.9 | 0.981 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)