| EpsZ |
| ID: | 1695368289 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 494 (449) | ||
| Sequences: | 3422 (2552.5) | ||
| Seq/Len: | 7.621 | ||
| Nf(neff/√len): | 120.5 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 323_V | 399_I | 5.820 | 1.00 |
| 302_R | 306_I | 5.514 | 1.00 |
| 356_S | 394_I | 5.359 | 1.00 |
| 326_A | 390_I | 4.929 | 1.00 |
| 330_T | 389_G | 4.107 | 1.00 |
| 408_V | 443_L | 3.993 | 1.00 |
| 313_L | 406_I | 3.764 | 1.00 |
| 340_L | 350_Y | 3.426 | 1.00 |
| 340_L | 348_P | 3.395 | 1.00 |
| 338_K | 350_Y | 3.390 | 1.00 |
| 304_F | 308_V | 3.293 | 1.00 |
| 323_V | 394_I | 3.283 | 1.00 |
| 459_E | 462_Y | 3.256 | 1.00 |
| 393_F | 397_F | 3.180 | 1.00 |
| 415_I | 480_L | 2.853 | 1.00 |
| 360_N | 364_L | 2.821 | 1.00 |
| 322_M | 326_A | 2.743 | 1.00 |
| 336_F | 352_L | 2.643 | 1.00 |
| 308_V | 415_I | 2.529 | 1.00 |
| 446_I | 450_S | 2.473 | 1.00 |
| 405_F | 443_L | 2.462 | 1.00 |
| 342_V | 348_P | 2.437 | 1.00 |
| 405_F | 415_I | 2.431 | 1.00 |
| 316_L | 320_L | 2.417 | 1.00 |
| 328_K | 333_G | 2.351 | 1.00 |
| 170_A | 194_Q | 2.295 | 1.00 |
| 327_V | 330_T | 2.263 | 1.00 |
| 310_A | 409_L | 2.260 | 1.00 |
| 328_K | 335_I | 2.204 | 1.00 |
| 233_E | 262_A | 2.204 | 1.00 |
| 170_A | 232_D | 2.182 | 1.00 |
| 312_A | 316_L | 2.170 | 1.00 |
| 309_S | 409_L | 2.168 | 1.00 |
| 324_A | 335_I | 2.158 | 1.00 |
| 358_V | 364_L | 2.144 | 1.00 |
| 124_T | 135_T | 2.111 | 1.00 |
| 383_H | 392_R | 2.096 | 1.00 |
| 113_S | 143_L | 2.046 | 1.00 |
| 394_I | 399_I | 2.024 | 1.00 |
| 48_V | 153_L | 2.014 | 1.00 |
| 313_L | 317_L | 1.999 | 1.00 |
| 305_D | 414_S | 1.989 | 1.00 |
| 334_P | 359_V | 1.985 | 1.00 |
| 171_V | 193_R | 1.970 | 1.00 |
| 184_G | 198_Y | 1.967 | 1.00 |
| 364_L | 367_K | 1.964 | 1.00 |
| 323_V | 354_F | 1.955 | 1.00 |
| 312_A | 405_F | 1.952 | 1.00 |
| 168_M | 194_Q | 1.924 | 1.00 |
| 338_K | 352_L | 1.917 | 1.00 |
| 360_N | 363_E | 1.913 | 1.00 |
| 421_P | 425_E | 1.901 | 1.00 |
| 406_I | 410_R | 1.901 | 1.00 |
| 352_L | 410_R | 1.898 | 1.00 |
| 317_L | 321_G | 1.897 | 1.00 |
| 390_I | 394_I | 1.879 | 1.00 |
| 306_I | 309_S | 1.844 | 1.00 |
| 416_V | 444_T | 1.820 | 1.00 |
| 342_V | 426_V | 1.808 | 1.00 |
| 320_L | 354_F | 1.786 | 1.00 |
| 422_V | 425_E | 1.779 | 1.00 |
| 337_F | 362_E | 1.767 | 1.00 |
| 421_P | 426_V | 1.733 | 1.00 |
| 421_P | 429_Y | 1.731 | 1.00 |
| 173_I | 184_G | 1.708 | 1.00 |
| 309_S | 313_L | 1.707 | 1.00 |
| 197_G | 217_G | 1.698 | 1.00 |
| 416_V | 480_L | 1.695 | 1.00 |
| 90_G | 95_L | 1.681 | 1.00 |
| 425_E | 429_Y | 1.655 | 1.00 |
| 64_S | 135_T | 1.639 | 1.00 |
| 172_L | 199_V | 1.637 | 1.00 |
| 466_Q | 470_H | 1.634 | 1.00 |
| 177_G | 180_G | 1.593 | 1.00 |
| 308_V | 312_A | 1.592 | 1.00 |
| 321_G | 325_L | 1.591 | 1.00 |
| 421_P | 457_F | 1.574 | 1.00 |
| 312_A | 415_I | 1.574 | 1.00 |
| 320_L | 336_F | 1.541 | 1.00 |
| 317_L | 320_L | 1.535 | 1.00 |
| 306_I | 310_A | 1.531 | 1.00 |
| 421_P | 460_W | 1.530 | 1.00 |
| 165_E | 192_R | 1.529 | 1.00 |
| 389_G | 392_R | 1.517 | 1.00 |
| 487_V | 492_G | 1.500 | 0.99 |
| 325_L | 329_F | 1.499 | 0.99 |
| 52_L | 153_L | 1.491 | 0.99 |
| 320_L | 406_I | 1.468 | 0.99 |
| 387_I | 392_R | 1.457 | 0.99 |
| 422_V | 457_F | 1.445 | 0.99 |
| 176_T | 180_G | 1.444 | 0.99 |
| 429_Y | 457_F | 1.442 | 0.99 |
| 318_P | 321_G | 1.438 | 0.99 |
| 319_L | 323_V | 1.438 | 0.99 |
| 446_I | 466_Q | 1.418 | 0.99 |
| 337_F | 422_V | 1.407 | 0.99 |
| 473_L | 477_L | 1.393 | 0.99 |
| 402_L | 405_F | 1.390 | 0.99 |
| 352_L | 407_N | 1.385 | 0.99 |
| 456_S | 460_W | 1.382 | 0.99 |
| 162_A | 165_E | 1.382 | 0.99 |
| 224_H | 228_N | 1.358 | 0.99 |
| 199_V | 225_I | 1.355 | 0.99 |
| 310_A | 314_W | 1.349 | 0.99 |
| 379_F | 421_P | 1.348 | 0.99 |
| 382_K | 494_S | 1.338 | 0.98 |
| 406_I | 412_E | 1.333 | 0.98 |
| 301_K | 305_D | 1.328 | 0.98 |
| 225_I | 229_T | 1.318 | 0.98 |
| 306_I | 409_L | 1.317 | 0.98 |
| 162_A | 167_P | 1.311 | 0.98 |
| 343_G | 441_P | 1.301 | 0.98 |
| 304_F | 473_L | 1.290 | 0.98 |
| 422_V | 426_V | 1.290 | 0.98 |
| 415_I | 443_L | 1.278 | 0.98 |
| 394_I | 397_F | 1.278 | 0.98 |
| 52_L | 56_V | 1.274 | 0.98 |
| 457_F | 460_W | 1.270 | 0.98 |
| 109_V | 158_F | 1.269 | 0.98 |
| 218_S | 221_D | 1.268 | 0.98 |
| 323_V | 326_A | 1.268 | 0.98 |
| 172_L | 231_V | 1.252 | 0.97 |
| 127_S | 135_T | 1.250 | 0.97 |
| 398_S | 452_R | 1.247 | 0.97 |
| 221_D | 224_H | 1.235 | 0.97 |
| 390_I | 393_F | 1.228 | 0.97 |
| 436_R | 460_W | 1.223 | 0.97 |
| 299_A | 303_L | 1.220 | 0.97 |
| 423_P | 427_A | 1.219 | 0.97 |
| 421_P | 461_M | 1.214 | 0.97 |
| 422_V | 429_Y | 1.213 | 0.97 |
| 302_R | 309_S | 1.208 | 0.97 |
| 330_T | 388_T | 1.207 | 0.97 |
| 421_P | 436_R | 1.204 | 0.96 |
| 477_L | 481_L | 1.201 | 0.96 |
| 358_V | 385_P | 1.191 | 0.96 |
| 478_R | 482_Q | 1.185 | 0.96 |
| 421_P | 447_W | 1.182 | 0.96 |
| 304_F | 477_L | 1.180 | 0.96 |
| 339_Q | 422_V | 1.179 | 0.96 |
| 300_M | 304_F | 1.177 | 0.96 |
| 216_M | 225_I | 1.176 | 0.96 |
| 451_G | 471_W | 1.175 | 0.96 |
| 484_V | 488_L | 1.170 | 0.96 |
| 485_P | 489_T | 1.161 | 0.95 |
| 480_L | 487_V | 1.159 | 0.95 |
| 197_G | 216_M | 1.156 | 0.95 |
| 328_K | 334_P | 1.156 | 0.95 |
| 330_T | 390_I | 1.155 | 0.95 |
| 176_T | 200_R | 1.153 | 0.95 |
| 368_L | 372_N | 1.153 | 0.95 |
| 379_F | 422_V | 1.149 | 0.95 |
| 326_A | 393_F | 1.148 | 0.95 |
| 398_S | 460_W | 1.146 | 0.95 |
| 358_V | 361_A | 1.142 | 0.95 |
| 132_T | 135_T | 1.142 | 0.95 |
| 227_R | 257_F | 1.134 | 0.95 |
| 339_Q | 362_E | 1.133 | 0.95 |
| 380_K | 425_E | 1.133 | 0.95 |
| 318_P | 322_M | 1.130 | 0.94 |
| 322_M | 325_L | 1.126 | 0.94 |
| 454_Q | 491_R | 1.123 | 0.94 |
| 173_I | 198_Y | 1.117 | 0.94 |
| 340_L | 424_T | 1.117 | 0.94 |
| 447_W | 492_G | 1.102 | 0.93 |
| 226_L | 259_V | 1.100 | 0.93 |
| 124_T | 127_S | 1.095 | 0.93 |
| 424_T | 428_K | 1.087 | 0.93 |
| 416_V | 449_V | 1.084 | 0.93 |
| 173_I | 180_G | 1.079 | 0.93 |
| 53_L | 146_F | 1.079 | 0.93 |
| 423_P | 426_V | 1.078 | 0.92 |
| 303_L | 306_I | 1.072 | 0.92 |
| 407_N | 412_E | 1.070 | 0.92 |
| 365_K | 424_T | 1.065 | 0.92 |
| 426_V | 437_L | 1.064 | 0.92 |
| 313_L | 409_L | 1.063 | 0.92 |
| 316_L | 319_L | 1.063 | 0.92 |
| 405_F | 480_L | 1.061 | 0.92 |
| 307_C | 311_A | 1.060 | 0.92 |
| 301_K | 414_S | 1.056 | 0.91 |
| 421_P | 424_T | 1.046 | 0.91 |
| 398_S | 451_G | 1.045 | 0.91 |
| 445_C | 448_Q | 1.041 | 0.91 |
| 141_P | 145_I | 1.040 | 0.91 |
| 320_L | 402_L | 1.038 | 0.91 |
| 439_V | 468_I | 1.037 | 0.90 |
| 196_L | 231_V | 1.037 | 0.90 |
| 398_S | 471_W | 1.035 | 0.90 |
| 89_T | 93_L | 1.034 | 0.90 |
| 172_L | 225_I | 1.029 | 0.90 |
| 426_V | 429_Y | 1.028 | 0.90 |
| 302_R | 305_D | 1.027 | 0.90 |
| 380_K | 447_W | 1.023 | 0.90 |
| 296_H | 299_A | 1.023 | 0.90 |
| 434_R | 437_L | 1.021 | 0.90 |
| 421_P | 437_L | 1.016 | 0.89 |
| 398_S | 487_V | 1.014 | 0.89 |
| 446_I | 463_L | 1.013 | 0.89 |
| 198_Y | 213_G | 1.008 | 0.89 |
| 183_T | 235_Y | 1.008 | 0.89 |
| 346_G | 437_L | 1.006 | 0.89 |
| 181_R | 185_E | 1.004 | 0.88 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3nklA | 2 | 0.2409 | 99 | 0.913 | Contact Map |
| 3keoA | 2 | 0.4008 | 98.1 | 0.934 | Contact Map |
| 2dt5A | 2 | 0.415 | 97.9 | 0.937 | Contact Map |
| 3wg9A | 4 | 0.417 | 97.9 | 0.938 | Contact Map |
| 2vt3A | 2 | 0.3988 | 97.7 | 0.94 | Contact Map |
| 2py6A | 1 | 0.5951 | 96.7 | 0.949 | Contact Map |
| 4m98A | 1 | 0.4028 | 96.1 | 0.953 | Contact Map |
| 4ea9A | 1 | 0.3846 | 91.5 | 0.961 | Contact Map |
| 4rv9A | 1 | 0.5223 | 88.8 | 0.963 | Contact Map |
| 3ff4A | 1 | 0.2368 | 82.4 | 0.967 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)