| P450_moudle4_P7026 |
| ID: | 1669123705 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 485 (473) | ||
| Sequences: | 20909 (13856.3) | ||
| Seq/Len: | 44.205 | ||
| Nf(neff/√len): | 637.1 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 332_A | 395_L | 5.566 | 1.00 |
| 385_N | 388_E | 4.295 | 1.00 |
| 291_A | 437_L | 3.885 | 1.00 |
| 303_K | 307_D | 3.875 | 1.00 |
| 307_D | 311_E | 3.817 | 1.00 |
| 301_V | 329_Y | 3.697 | 1.00 |
| 294_E | 341_H | 3.690 | 1.00 |
| 385_N | 390_N | 3.683 | 1.00 |
| 229_M | 266_M | 3.386 | 1.00 |
| 291_A | 337_T | 3.358 | 1.00 |
| 305_V | 333_V | 3.218 | 1.00 |
| 355_S | 365_E | 3.208 | 1.00 |
| 304_R | 392_D | 3.135 | 1.00 |
| 305_V | 441_M | 3.023 | 1.00 |
| 331_E | 434_M | 3.009 | 1.00 |
| 306_Q | 445_D | 2.950 | 1.00 |
| 378_R | 387_S | 2.879 | 1.00 |
| 287_M | 433_M | 2.860 | 1.00 |
| 440_L | 446_W | 2.764 | 1.00 |
| 291_A | 333_V | 2.693 | 1.00 |
| 303_K | 445_D | 2.649 | 1.00 |
| 304_R | 329_Y | 2.613 | 1.00 |
| 305_V | 330_L | 2.592 | 1.00 |
| 380_P | 386_P | 2.587 | 1.00 |
| 144_S | 292_M | 2.573 | 1.00 |
| 144_S | 288_V | 2.555 | 1.00 |
| 334_C | 433_M | 2.507 | 1.00 |
| 306_Q | 310_E | 2.475 | 1.00 |
| 228_R | 247_D | 2.427 | 1.00 |
| 28_T | 32_H | 2.387 | 1.00 |
| 226_K | 230_N | 2.380 | 1.00 |
| 364_P | 367_A | 2.347 | 1.00 |
| 15_S | 18_F | 2.324 | 1.00 |
| 147_T | 436_I | 2.293 | 1.00 |
| 291_A | 295_I | 2.258 | 1.00 |
| 302_M | 445_D | 2.242 | 1.00 |
| 303_K | 306_Q | 2.233 | 1.00 |
| 329_Y | 392_D | 2.232 | 1.00 |
| 447_K | 475_T | 2.214 | 1.00 |
| 37_I | 50_V | 2.212 | 1.00 |
| 222_E | 226_K | 2.211 | 1.00 |
| 11_P | 14_G | 2.207 | 1.00 |
| 317_N | 323_H | 2.180 | 1.00 |
| 357_T | 362_T | 2.154 | 1.00 |
| 445_D | 475_T | 2.133 | 1.00 |
| 302_M | 306_Q | 2.091 | 1.00 |
| 352_P | 363_V | 2.085 | 1.00 |
| 56_A | 376_I | 2.077 | 1.00 |
| 355_S | 362_T | 2.074 | 1.00 |
| 287_M | 343_P | 2.071 | 1.00 |
| 357_T | 360_G | 2.050 | 1.00 |
| 296_F | 302_M | 2.016 | 1.00 |
| 447_K | 473_V | 2.015 | 1.00 |
| 374_W | 378_R | 1.986 | 1.00 |
| 50_V | 59_I | 1.974 | 1.00 |
| 340_L | 378_R | 1.970 | 1.00 |
| 13_V | 17_P | 1.970 | 1.00 |
| 95_R | 99_R | 1.969 | 1.00 |
| 309_L | 438_A | 1.968 | 1.00 |
| 380_P | 387_S | 1.939 | 1.00 |
| 294_E | 298_D | 1.930 | 1.00 |
| 333_V | 389_F | 1.877 | 1.00 |
| 6_G | 15_S | 1.875 | 1.00 |
| 26_E | 29_K | 1.867 | 1.00 |
| 312_I | 327_L | 1.864 | 1.00 |
| 288_V | 436_I | 1.860 | 1.00 |
| 29_K | 32_H | 1.851 | 1.00 |
| 295_I | 301_V | 1.839 | 1.00 |
| 38_F | 49_V | 1.830 | 1.00 |
| 66_S | 354_K | 1.811 | 1.00 |
| 222_E | 270_K | 1.809 | 1.00 |
| 301_V | 333_V | 1.801 | 1.00 |
| 10_L | 13_V | 1.794 | 1.00 |
| 292_M | 440_L | 1.789 | 1.00 |
| 335_K | 413_F | 1.765 | 1.00 |
| 429_G | 433_M | 1.739 | 1.00 |
| 308_E | 327_L | 1.737 | 1.00 |
| 219_K | 223_P | 1.734 | 1.00 |
| 298_D | 301_V | 1.723 | 1.00 |
| 292_M | 437_L | 1.694 | 1.00 |
| 295_I | 302_M | 1.685 | 1.00 |
| 294_E | 340_L | 1.656 | 1.00 |
| 29_K | 33_Q | 1.652 | 1.00 |
| 295_I | 305_V | 1.651 | 1.00 |
| 147_T | 151_V | 1.650 | 1.00 |
| 351_A | 366_G | 1.631 | 1.00 |
| 222_E | 266_M | 1.626 | 1.00 |
| 226_K | 266_M | 1.625 | 1.00 |
| 309_L | 327_L | 1.618 | 1.00 |
| 354_K | 365_E | 1.615 | 1.00 |
| 320_E | 323_H | 1.591 | 1.00 |
| 92_N | 95_R | 1.586 | 1.00 |
| 140_I | 292_M | 1.582 | 1.00 |
| 288_V | 433_M | 1.578 | 1.00 |
| 67_F | 356_C | 1.577 | 1.00 |
| 306_Q | 477_R | 1.575 | 1.00 |
| 55_L | 359_G | 1.573 | 1.00 |
| 98_L | 272_L | 1.572 | 1.00 |
| 41_Y | 46_L | 1.561 | 1.00 |
| 39_K | 46_L | 1.551 | 1.00 |
| 422_R | 426_I | 1.549 | 1.00 |
| 28_T | 375_A | 1.546 | 1.00 |
| 226_K | 229_M | 1.545 | 1.00 |
| 48_I | 363_V | 1.544 | 1.00 |
| 223_P | 227_E | 1.535 | 1.00 |
| 294_E | 297_R | 1.526 | 1.00 |
| 310_E | 442_H | 1.514 | 1.00 |
| 310_E | 477_R | 1.510 | 1.00 |
| 333_V | 337_T | 1.510 | 1.00 |
| 10_L | 17_P | 1.495 | 1.00 |
| 54_E | 57_K | 1.493 | 1.00 |
| 265_T | 268_Q | 1.484 | 1.00 |
| 139_D | 142_E | 1.481 | 1.00 |
| 36_P | 52_S | 1.481 | 1.00 |
| 331_E | 335_K | 1.476 | 1.00 |
| 131_Y | 444_F | 1.466 | 1.00 |
| 48_I | 367_A | 1.460 | 1.00 |
| 306_Q | 441_M | 1.451 | 1.00 |
| 293_T | 297_R | 1.449 | 1.00 |
| 159_K | 227_E | 1.444 | 1.00 |
| 380_P | 384_E | 1.438 | 1.00 |
| 332_A | 392_D | 1.437 | 1.00 |
| 50_V | 56_A | 1.421 | 1.00 |
| 152_F | 280_G | 1.419 | 1.00 |
| 155_I | 428_L | 1.418 | 1.00 |
| 131_Y | 443_S | 1.411 | 1.00 |
| 52_S | 55_L | 1.411 | 1.00 |
| 215_K | 219_K | 1.408 | 1.00 |
| 336_E | 339_R | 1.406 | 0.99 |
| 6_G | 34_Y | 1.401 | 0.99 |
| 446_W | 474_P | 1.380 | 0.99 |
| 32_H | 380_P | 1.377 | 0.99 |
| 430_E | 434_M | 1.361 | 0.99 |
| 124_R | 439_S | 1.348 | 0.99 |
| 26_E | 30_I | 1.347 | 0.99 |
| 338_F | 413_F | 1.345 | 0.99 |
| 93_A | 96_R | 1.340 | 0.99 |
| 377_Q | 415_P | 1.335 | 0.99 |
| 338_F | 415_P | 1.328 | 0.99 |
| 309_L | 330_L | 1.324 | 0.99 |
| 50_V | 55_L | 1.307 | 0.99 |
| 291_A | 389_F | 1.304 | 0.99 |
| 296_F | 472_A | 1.285 | 0.99 |
| 328_K | 332_A | 1.279 | 0.99 |
| 299_Q | 303_K | 1.278 | 0.99 |
| 335_K | 430_E | 1.271 | 0.99 |
| 9_G | 15_S | 1.270 | 0.99 |
| 304_R | 307_D | 1.268 | 0.99 |
| 352_P | 366_G | 1.266 | 0.99 |
| 377_Q | 413_F | 1.258 | 0.99 |
| 390_N | 396_N | 1.256 | 0.99 |
| 373_V | 414_L | 1.254 | 0.99 |
| 218_D | 270_K | 1.251 | 0.99 |
| 97_N | 268_Q | 1.229 | 0.98 |
| 287_M | 337_T | 1.214 | 0.98 |
| 112_L | 431_K | 1.207 | 0.98 |
| 22_N | 29_K | 1.205 | 0.98 |
| 419_G | 423_C | 1.198 | 0.98 |
| 323_H | 326_K | 1.194 | 0.98 |
| 93_A | 97_N | 1.186 | 0.98 |
| 148_V | 284_T | 1.185 | 0.98 |
| 152_F | 156_I | 1.184 | 0.98 |
| 211_K | 215_K | 1.183 | 0.98 |
| 351_A | 368_T | 1.181 | 0.98 |
| 37_I | 55_L | 1.173 | 0.98 |
| 22_N | 25_Q | 1.173 | 0.98 |
| 355_S | 364_P | 1.167 | 0.97 |
| 428_L | 432_M | 1.164 | 0.97 |
| 416_F | 419_G | 1.160 | 0.97 |
| 116_H | 120_R | 1.160 | 0.97 |
| 337_T | 389_F | 1.160 | 0.97 |
| 218_D | 267_T | 1.160 | 0.97 |
| 387_S | 390_N | 1.158 | 0.97 |
| 384_E | 390_N | 1.151 | 0.97 |
| 227_E | 231_S | 1.147 | 0.97 |
| 292_M | 441_M | 1.145 | 0.97 |
| 30_I | 38_F | 1.143 | 0.97 |
| 60_T | 371_V | 1.143 | 0.97 |
| 8_K | 15_S | 1.140 | 0.97 |
| 296_F | 446_W | 1.134 | 0.97 |
| 286_A | 343_P | 1.127 | 0.97 |
| 56_A | 371_V | 1.122 | 0.97 |
| 228_R | 231_S | 1.120 | 0.96 |
| 223_P | 226_K | 1.117 | 0.96 |
| 301_V | 390_N | 1.116 | 0.96 |
| 58_V | 67_F | 1.115 | 0.96 |
| 313_V | 442_H | 1.105 | 0.96 |
| 329_Y | 333_V | 1.104 | 0.96 |
| 230_N | 233_S | 1.101 | 0.96 |
| 330_L | 333_V | 1.100 | 0.96 |
| 144_S | 289_E | 1.093 | 0.96 |
| 309_L | 441_M | 1.093 | 0.96 |
| 250_Q | 254_E | 1.082 | 0.96 |
| 307_D | 310_E | 1.081 | 0.95 |
| 148_V | 152_F | 1.081 | 0.95 |
| 264_I | 268_Q | 1.079 | 0.95 |
| 140_I | 472_A | 1.075 | 0.95 |
| 324_I | 434_M | 1.074 | 0.95 |
| 11_P | 17_P | 1.072 | 0.95 |
| 379_D | 382_H | 1.068 | 0.95 |
| 291_A | 334_C | 1.067 | 0.95 |
| 54_E | 381_R | 1.063 | 0.95 |
| 55_L | 358_V | 1.062 | 0.95 |
| 338_F | 343_P | 1.062 | 0.95 |
| 363_V | 369_I | 1.061 | 0.95 |
| 121_H | 154_N | 1.061 | 0.95 |
| 247_D | 250_Q | 1.059 | 0.95 |
| 305_V | 329_Y | 1.056 | 0.95 |
| 149_L | 281_T | 1.055 | 0.95 |
| 94_N | 268_Q | 1.045 | 0.94 |
| 330_L | 434_M | 1.044 | 0.94 |
| 439_S | 442_H | 1.043 | 0.94 |
| 352_P | 365_E | 1.036 | 0.94 |
| 128_K | 131_Y | 1.035 | 0.94 |
| 224_T | 227_E | 1.035 | 0.94 |
| 3_L | 364_P | 1.034 | 0.94 |
| 288_V | 292_M | 1.030 | 0.94 |
| 300_K | 304_R | 1.029 | 0.94 |
| 425_G | 429_G | 1.029 | 0.94 |
| 250_Q | 253_L | 1.024 | 0.93 |
| 352_P | 355_S | 1.019 | 0.93 |
| 329_Y | 391_P | 1.019 | 0.93 |
| 300_K | 303_K | 1.017 | 0.93 |
| 246_K | 254_E | 1.017 | 0.93 |
| 107_L | 112_L | 1.014 | 0.93 |
| 352_P | 364_P | 1.005 | 0.93 |
| 232_R | 254_E | 1.004 | 0.93 |
| 77_L | 82_G | 1.003 | 0.93 |
| 51_N | 379_D | 1.002 | 0.92 |
| 284_T | 433_M | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4r21A | 2 | 0.866 | 100 | 0.106 | Contact Map |
| 3tbgA | 4 | 0.9423 | 100 | 0.111 | Contact Map |
| 3czhA | 2 | 0.9485 | 100 | 0.114 | Contact Map |
| 3pm0A | 1 | 0.9216 | 100 | 0.114 | Contact Map |
| 2hi4A | 1 | 0.9505 | 100 | 0.126 | Contact Map |
| 1r9oA | 1 | 0.9175 | 100 | 0.127 | Contact Map |
| 3swzA | 4 | 0.9505 | 100 | 0.128 | Contact Map |
| 3k9vA | 2 | 0.9196 | 100 | 0.132 | Contact Map |
| 2fdvA | 4 | 0.9381 | 100 | 0.134 | Contact Map |
| 1po5A | 1 | 0.9361 | 100 | 0.134 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)