| P450_moudle_P7019-25_trimal |
| ID: | 1668520609 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 458 (427) | ||
| Sequences: | 24884 (17154.6) | ||
| Seq/Len: | 58.276 | ||
| Nf(neff/√len): | 830.2 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 324_A | 385_L | 5.025 | 1.00 |
| 375_N | 378_E | 3.752 | 1.00 |
| 296_K | 300_D | 3.553 | 1.00 |
| 424_D | 450_T | 3.490 | 1.00 |
| 294_V | 321_Y | 3.438 | 1.00 |
| 287_E | 333_H | 3.392 | 1.00 |
| 300_D | 304_E | 3.383 | 1.00 |
| 284_A | 416_L | 3.329 | 1.00 |
| 426_K | 448_V | 3.288 | 1.00 |
| 297_R | 382_D | 3.203 | 1.00 |
| 346_S | 356_E | 3.145 | 1.00 |
| 280_M | 412_M | 3.041 | 1.00 |
| 375_N | 380_N | 3.013 | 1.00 |
| 323_E | 413_M | 2.963 | 1.00 |
| 229_M | 259_M | 2.957 | 1.00 |
| 298_V | 325_V | 2.930 | 1.00 |
| 228_R | 241_D | 2.856 | 1.00 |
| 355_P | 358_A | 2.826 | 1.00 |
| 426_K | 450_T | 2.789 | 1.00 |
| 299_Q | 424_D | 2.748 | 1.00 |
| 284_A | 329_T | 2.671 | 1.00 |
| 298_V | 420_M | 2.654 | 1.00 |
| 299_Q | 452_R | 2.633 | 1.00 |
| 369_R | 377_S | 2.603 | 1.00 |
| 164_T | 415_I | 2.506 | 1.00 |
| 297_R | 321_Y | 2.440 | 1.00 |
| 348_T | 353_T | 2.426 | 1.00 |
| 371_P | 376_P | 2.353 | 1.00 |
| 284_A | 325_V | 2.350 | 1.00 |
| 222_E | 226_K | 2.349 | 1.00 |
| 296_K | 424_D | 2.334 | 1.00 |
| 419_L | 425_W | 2.326 | 1.00 |
| 39_P | 42_G | 2.308 | 1.00 |
| 303_E | 452_R | 2.295 | 1.00 |
| 298_V | 322_L | 2.282 | 1.00 |
| 226_K | 259_M | 2.218 | 1.00 |
| 226_K | 230_S | 2.156 | 1.00 |
| 161_S | 281_V | 2.154 | 1.00 |
| 346_S | 353_T | 2.124 | 1.00 |
| 299_Q | 303_E | 2.117 | 1.00 |
| 161_S | 285_M | 2.075 | 1.00 |
| 326_C | 412_M | 2.065 | 1.00 |
| 343_A | 354_V | 2.054 | 1.00 |
| 295_M | 424_D | 2.048 | 1.00 |
| 281_V | 415_I | 2.038 | 1.00 |
| 43_S | 46_F | 2.019 | 1.00 |
| 222_E | 263_K | 2.011 | 1.00 |
| 295_M | 299_Q | 2.002 | 1.00 |
| 284_A | 288_I | 2.000 | 1.00 |
| 77_V | 86_I | 1.988 | 1.00 |
| 332_L | 369_R | 1.985 | 1.00 |
| 348_T | 351_G | 1.955 | 1.00 |
| 296_K | 299_Q | 1.927 | 1.00 |
| 365_W | 369_R | 1.920 | 1.00 |
| 321_Y | 382_D | 1.916 | 1.00 |
| 302_L | 417_A | 1.915 | 1.00 |
| 94_F | 347_C | 1.912 | 1.00 |
| 287_E | 291_D | 1.906 | 1.00 |
| 219_K | 223_P | 1.900 | 1.00 |
| 289_F | 295_M | 1.894 | 1.00 |
| 325_V | 379_F | 1.881 | 1.00 |
| 65_I | 77_V | 1.847 | 1.00 |
| 120_L | 265_L | 1.846 | 1.00 |
| 83_A | 367_I | 1.820 | 1.00 |
| 144_R | 418_S | 1.817 | 1.00 |
| 215_K | 219_K | 1.807 | 1.00 |
| 312_E | 315_H | 1.765 | 1.00 |
| 41_V | 45_P | 1.759 | 1.00 |
| 305_I | 319_L | 1.680 | 1.00 |
| 222_E | 259_M | 1.668 | 1.00 |
| 425_W | 449_P | 1.653 | 1.00 |
| 285_M | 419_L | 1.652 | 1.00 |
| 291_D | 294_V | 1.651 | 1.00 |
| 288_I | 294_V | 1.650 | 1.00 |
| 258_T | 261_Q | 1.629 | 1.00 |
| 280_M | 335_P | 1.627 | 1.00 |
| 294_V | 325_V | 1.626 | 1.00 |
| 302_L | 319_L | 1.622 | 1.00 |
| 303_E | 421_H | 1.606 | 1.00 |
| 309_N | 315_H | 1.597 | 1.00 |
| 56_T | 60_H | 1.592 | 1.00 |
| 287_E | 332_L | 1.586 | 1.00 |
| 371_P | 377_S | 1.584 | 1.00 |
| 408_G | 412_M | 1.573 | 1.00 |
| 34_G | 43_S | 1.566 | 1.00 |
| 66_F | 76_V | 1.545 | 1.00 |
| 401_R | 405_I | 1.544 | 1.00 |
| 288_I | 298_V | 1.532 | 1.00 |
| 345_I | 356_E | 1.520 | 1.00 |
| 38_L | 41_V | 1.519 | 1.00 |
| 223_P | 227_E | 1.508 | 1.00 |
| 327_K | 392_F | 1.503 | 1.00 |
| 287_E | 329_T | 1.491 | 1.00 |
| 82_L | 350_G | 1.488 | 1.00 |
| 302_L | 322_L | 1.482 | 1.00 |
| 324_A | 382_D | 1.468 | 1.00 |
| 346_S | 355_P | 1.466 | 1.00 |
| 301_E | 319_L | 1.451 | 1.00 |
| 244_Q | 248_E | 1.451 | 1.00 |
| 79_S | 82_L | 1.438 | 1.00 |
| 285_M | 447_A | 1.436 | 1.00 |
| 75_I | 358_A | 1.435 | 1.00 |
| 226_K | 229_M | 1.433 | 1.00 |
| 368_Q | 394_P | 1.433 | 1.00 |
| 172_I | 407_L | 1.421 | 1.00 |
| 328_E | 331_R | 1.414 | 1.00 |
| 281_V | 412_M | 1.414 | 1.00 |
| 38_L | 45_P | 1.404 | 0.99 |
| 330_F | 394_P | 1.403 | 0.99 |
| 323_E | 327_K | 1.393 | 0.99 |
| 224_T | 227_E | 1.381 | 0.99 |
| 218_D | 260_T | 1.378 | 0.99 |
| 286_T | 290_R | 1.373 | 0.99 |
| 67_K | 73_L | 1.373 | 0.99 |
| 84_K | 88_A | 1.370 | 0.99 |
| 143_V | 164_T | 1.369 | 0.99 |
| 380_N | 386_S | 1.367 | 0.99 |
| 288_I | 295_M | 1.342 | 0.99 |
| 325_V | 329_T | 1.341 | 0.99 |
| 133_L | 410_K | 1.339 | 0.99 |
| 166_L | 274_T | 1.337 | 0.99 |
| 117_R | 121_R | 1.334 | 0.99 |
| 156_D | 159_E | 1.333 | 0.99 |
| 297_R | 300_D | 1.332 | 0.99 |
| 176_K | 227_E | 1.328 | 0.99 |
| 169_F | 273_G | 1.313 | 0.99 |
| 190_I | 216_Q | 1.310 | 0.99 |
| 119_N | 261_Q | 1.309 | 0.99 |
| 141_H | 171_N | 1.307 | 0.99 |
| 152_G | 451_K | 1.306 | 0.99 |
| 77_V | 82_L | 1.295 | 0.99 |
| 164_T | 168_V | 1.294 | 0.99 |
| 170_T | 178_F | 1.294 | 0.99 |
| 92_E | 345_I | 1.294 | 0.99 |
| 93_S | 345_I | 1.292 | 0.99 |
| 83_A | 362_V | 1.285 | 0.99 |
| 299_Q | 420_M | 1.284 | 0.99 |
| 227_E | 231_R | 1.283 | 0.99 |
| 285_M | 416_L | 1.281 | 0.99 |
| 368_Q | 392_F | 1.279 | 0.99 |
| 327_K | 409_E | 1.278 | 0.99 |
| 157_I | 285_M | 1.267 | 0.99 |
| 169_F | 173_I | 1.240 | 0.98 |
| 289_F | 447_A | 1.229 | 0.98 |
| 81_E | 84_K | 1.225 | 0.98 |
| 171_N | 177_G | 1.224 | 0.98 |
| 289_F | 425_W | 1.217 | 0.98 |
| 287_E | 290_R | 1.210 | 0.98 |
| 69_Y | 73_L | 1.206 | 0.98 |
| 329_T | 379_F | 1.201 | 0.98 |
| 218_D | 263_K | 1.199 | 0.98 |
| 142_E | 171_N | 1.198 | 0.98 |
| 95_A | 344_P | 1.195 | 0.98 |
| 407_L | 411_M | 1.188 | 0.98 |
| 37_G | 43_S | 1.186 | 0.98 |
| 398_G | 402_C | 1.178 | 0.98 |
| 284_A | 326_C | 1.175 | 0.98 |
| 280_M | 329_T | 1.172 | 0.98 |
| 306_V | 421_H | 1.171 | 0.98 |
| 64_P | 79_S | 1.169 | 0.97 |
| 241_D | 244_Q | 1.163 | 0.97 |
| 293_K | 297_R | 1.148 | 0.97 |
| 75_I | 354_V | 1.147 | 0.97 |
| 57_K | 60_H | 1.144 | 0.97 |
| 77_V | 83_A | 1.128 | 0.97 |
| 238_G | 248_E | 1.119 | 0.96 |
| 409_E | 413_M | 1.117 | 0.96 |
| 157_I | 447_A | 1.114 | 0.96 |
| 330_F | 392_F | 1.113 | 0.96 |
| 289_F | 427_L | 1.112 | 0.96 |
| 54_E | 58_I | 1.109 | 0.96 |
| 58_I | 66_F | 1.105 | 0.96 |
| 165_V | 277_T | 1.095 | 0.96 |
| 374_W | 384_F | 1.077 | 0.95 |
| 227_E | 230_S | 1.077 | 0.95 |
| 298_V | 321_Y | 1.070 | 0.95 |
| 353_T | 356_E | 1.068 | 0.95 |
| 161_S | 282_E | 1.065 | 0.95 |
| 124_L | 403_P | 1.061 | 0.95 |
| 116_N | 119_N | 1.056 | 0.95 |
| 315_H | 318_K | 1.053 | 0.95 |
| 257_I | 261_Q | 1.051 | 0.94 |
| 152_G | 423_F | 1.050 | 0.94 |
| 218_D | 222_E | 1.045 | 0.94 |
| 294_V | 297_R | 1.042 | 0.94 |
| 418_S | 422_S | 1.036 | 0.94 |
| 284_A | 379_F | 1.033 | 0.94 |
| 228_R | 244_Q | 1.029 | 0.94 |
| 364_V | 393_L | 1.029 | 0.94 |
| 50_N | 53_Q | 1.027 | 0.94 |
| 395_F | 398_G | 1.026 | 0.94 |
| 128_L | 403_P | 1.024 | 0.93 |
| 230_S | 233_T | 1.024 | 0.93 |
| 370_D | 373_H | 1.022 | 0.93 |
| 316_I | 413_M | 1.022 | 0.93 |
| 343_A | 357_G | 1.020 | 0.93 |
| 320_K | 324_A | 1.018 | 0.93 |
| 78_N | 363_N | 1.017 | 0.93 |
| 293_K | 296_K | 1.016 | 0.93 |
| 322_L | 325_V | 1.012 | 0.93 |
| 156_D | 444_P | 1.011 | 0.93 |
| 294_V | 382_D | 1.010 | 0.93 |
| 141_H | 145_K | 1.004 | 0.93 |
| 418_S | 421_H | 1.004 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2ve3A | 2 | 0.9105 | 100 | 0.204 | Contact Map |
| 4lxjA | 1 | 0.9869 | 100 | 0.209 | Contact Map |
| 3czhA | 2 | 0.9258 | 100 | 0.224 | Contact Map |
| 3tbgA | 4 | 0.917 | 100 | 0.227 | Contact Map |
| 4r21A | 2 | 0.8603 | 100 | 0.228 | Contact Map |
| 1r9oA | 1 | 0.8974 | 100 | 0.228 | Contact Map |
| 3nxuA | 2 | 0.917 | 100 | 0.229 | Contact Map |
| 2cibA | 1 | 0.8581 | 100 | 0.23 | Contact Map |
| 3pm0A | 1 | 0.8865 | 100 | 0.23 | Contact Map |
| 3k9vA | 2 | 0.9148 | 100 | 0.23 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)