| P450_moudle_P7006 |
| ID: | 1668406792 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 520 (476) | ||
| Sequences: | 14741 (10063.8) | ||
| Seq/Len: | 30.968 | ||
| Nf(neff/√len): | 461.3 |
HHblits Results: (2016_02)
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 367_A | 430_L | 5.449 | 1.00 |
| 420_N | 423_E | 4.593 | 1.00 |
| 338_K | 342_D | 3.892 | 1.00 |
| 326_A | 472_L | 3.822 | 1.00 |
| 342_D | 346_E | 3.821 | 1.00 |
| 420_N | 425_N | 3.757 | 1.00 |
| 329_E | 376_H | 3.736 | 1.00 |
| 390_S | 400_E | 3.680 | 1.00 |
| 340_V | 368_V | 3.666 | 1.00 |
| 336_V | 364_Y | 3.644 | 1.00 |
| 340_V | 476_M | 3.482 | 1.00 |
| 326_A | 372_T | 3.473 | 1.00 |
| 341_Q | 480_D | 2.987 | 1.00 |
| 339_R | 427_D | 2.900 | 1.00 |
| 475_L | 481_W | 2.809 | 1.00 |
| 366_E | 469_M | 2.805 | 1.00 |
| 413_R | 422_S | 2.680 | 1.00 |
| 50_S | 53_F | 2.633 | 1.00 |
| 179_S | 323_V | 2.581 | 1.00 |
| 179_S | 327_M | 2.525 | 1.00 |
| 182_T | 471_I | 2.509 | 1.00 |
| 390_S | 397_T | 2.501 | 1.00 |
| 340_V | 365_L | 2.481 | 1.00 |
| 63_T | 67_H | 2.480 | 1.00 |
| 322_M | 468_M | 2.475 | 1.00 |
| 339_R | 364_Y | 2.469 | 1.00 |
| 415_P | 421_P | 2.435 | 1.00 |
| 392_T | 397_T | 2.409 | 1.00 |
| 264_M | 301_M | 2.368 | 1.00 |
| 338_K | 480_D | 2.352 | 1.00 |
| 257_E | 261_K | 2.331 | 1.00 |
| 338_K | 341_Q | 2.312 | 1.00 |
| 364_Y | 427_D | 2.264 | 1.00 |
| 261_K | 265_N | 2.219 | 1.00 |
| 326_A | 368_V | 2.204 | 1.00 |
| 392_T | 395_G | 2.200 | 1.00 |
| 91_A | 411_I | 2.197 | 1.00 |
| 46_P | 49_G | 2.184 | 1.00 |
| 341_Q | 345_E | 2.178 | 1.00 |
| 337_M | 480_D | 2.165 | 1.00 |
| 369_C | 468_M | 2.155 | 1.00 |
| 263_R | 282_D | 2.152 | 1.00 |
| 352_N | 358_H | 2.145 | 1.00 |
| 375_L | 413_R | 2.140 | 1.00 |
| 326_A | 330_I | 2.089 | 1.00 |
| 323_V | 471_I | 2.068 | 1.00 |
| 368_V | 424_F | 2.040 | 1.00 |
| 61_E | 64_K | 2.032 | 1.00 |
| 156_H | 189_N | 2.028 | 1.00 |
| 387_P | 398_V | 1.997 | 1.00 |
| 40_P | 50_S | 1.992 | 1.00 |
| 90_L | 394_G | 1.958 | 1.00 |
| 331_F | 337_M | 1.953 | 1.00 |
| 64_K | 67_H | 1.952 | 1.00 |
| 250_K | 254_K | 1.933 | 1.00 |
| 254_K | 258_P | 1.932 | 1.00 |
| 386_A | 401_G | 1.914 | 1.00 |
| 40_P | 69_Y | 1.913 | 1.00 |
| 45_L | 48_V | 1.911 | 1.00 |
| 159_R | 474_S | 1.881 | 1.00 |
| 370_K | 448_F | 1.859 | 1.00 |
| 194_K | 262_E | 1.845 | 1.00 |
| 337_M | 341_Q | 1.844 | 1.00 |
| 330_I | 336_V | 1.840 | 1.00 |
| 263_R | 285_Q | 1.824 | 1.00 |
| 336_V | 368_V | 1.821 | 1.00 |
| 158_V | 182_T | 1.806 | 1.00 |
| 166_Y | 478_S | 1.806 | 1.00 |
| 482_K | 508_V | 1.800 | 1.00 |
| 63_T | 410_A | 1.798 | 1.00 |
| 322_M | 378_P | 1.787 | 1.00 |
| 354_V | 473_A | 1.787 | 1.00 |
| 389_K | 400_E | 1.776 | 1.00 |
| 329_E | 333_D | 1.775 | 1.00 |
| 85_V | 94_I | 1.771 | 1.00 |
| 175_I | 327_M | 1.761 | 1.00 |
| 323_V | 468_M | 1.748 | 1.00 |
| 409_W | 413_R | 1.742 | 1.00 |
| 174_D | 177_E | 1.723 | 1.00 |
| 399_P | 402_A | 1.721 | 1.00 |
| 333_D | 336_V | 1.720 | 1.00 |
| 73_F | 84_V | 1.710 | 1.00 |
| 48_V | 52_P | 1.685 | 1.00 |
| 258_P | 262_E | 1.682 | 1.00 |
| 74_K | 81_L | 1.679 | 1.00 |
| 464_G | 468_M | 1.679 | 1.00 |
| 482_K | 510_T | 1.676 | 1.00 |
| 265_N | 269_T | 1.676 | 1.00 |
| 343_E | 362_L | 1.674 | 1.00 |
| 85_V | 91_A | 1.665 | 1.00 |
| 480_D | 510_T | 1.654 | 1.00 |
| 64_K | 68_Q | 1.652 | 1.00 |
| 45_L | 52_P | 1.647 | 1.00 |
| 102_F | 391_C | 1.631 | 1.00 |
| 345_E | 512_R | 1.609 | 1.00 |
| 457_R | 461_I | 1.595 | 1.00 |
| 166_Y | 479_F | 1.584 | 1.00 |
| 76_Y | 81_L | 1.582 | 1.00 |
| 209_N | 251_Q | 1.582 | 1.00 |
| 257_E | 305_K | 1.577 | 1.00 |
| 344_L | 362_L | 1.576 | 1.00 |
| 329_E | 332_R | 1.573 | 1.00 |
| 341_Q | 512_R | 1.562 | 1.00 |
| 72_I | 85_V | 1.559 | 1.00 |
| 327_M | 475_L | 1.556 | 1.00 |
| 347_I | 362_L | 1.546 | 1.00 |
| 72_I | 90_L | 1.536 | 1.00 |
| 182_T | 186_V | 1.532 | 1.00 |
| 344_L | 473_A | 1.532 | 1.00 |
| 415_P | 422_S | 1.526 | 1.00 |
| 162_V | 474_S | 1.526 | 1.00 |
| 331_F | 507_A | 1.526 | 1.00 |
| 190_I | 463_L | 1.526 | 1.00 |
| 373_F | 448_F | 1.522 | 1.00 |
| 329_E | 375_L | 1.518 | 1.00 |
| 341_Q | 476_M | 1.509 | 1.00 |
| 101_S | 389_K | 1.494 | 1.00 |
| 373_F | 450_P | 1.493 | 1.00 |
| 328_T | 332_R | 1.476 | 1.00 |
| 184_L | 316_T | 1.460 | 1.00 |
| 77_L | 82_H | 1.458 | 1.00 |
| 330_I | 337_M | 1.457 | 1.00 |
| 212_K | 216_R | 1.453 | 1.00 |
| 345_E | 477_H | 1.435 | 1.00 |
| 330_I | 340_V | 1.434 | 1.00 |
| 359_I | 469_M | 1.432 | 1.00 |
| 83_I | 402_A | 1.421 | 1.00 |
| 158_V | 162_V | 1.379 | 0.99 |
| 390_S | 399_P | 1.377 | 0.99 |
| 85_V | 90_L | 1.366 | 0.99 |
| 162_V | 479_F | 1.361 | 0.99 |
| 368_V | 372_T | 1.353 | 0.99 |
| 370_K | 465_E | 1.350 | 0.99 |
| 157_E | 195_G | 1.349 | 0.99 |
| 247_D | 251_Q | 1.329 | 0.99 |
| 90_L | 393_V | 1.328 | 0.99 |
| 71_P | 87_S | 1.325 | 0.99 |
| 38_L | 82_H | 1.323 | 0.99 |
| 188_T | 196_F | 1.320 | 0.99 |
| 355_E | 358_H | 1.305 | 0.99 |
| 147_L | 466_K | 1.297 | 0.99 |
| 61_E | 65_I | 1.291 | 0.99 |
| 267_R | 289_E | 1.289 | 0.99 |
| 366_E | 370_K | 1.284 | 0.99 |
| 319_T | 468_M | 1.282 | 0.99 |
| 322_M | 372_T | 1.281 | 0.99 |
| 261_K | 301_M | 1.276 | 0.99 |
| 367_A | 427_D | 1.274 | 0.99 |
| 142_L | 459_P | 1.270 | 0.99 |
| 183_V | 319_T | 1.269 | 0.99 |
| 408_V | 449_L | 1.266 | 0.99 |
| 186_V | 467_M | 1.260 | 0.99 |
| 327_M | 472_L | 1.259 | 0.99 |
| 465_E | 469_M | 1.250 | 0.99 |
| 412_Q | 448_F | 1.229 | 0.98 |
| 133_L | 307_L | 1.226 | 0.98 |
| 87_S | 90_L | 1.221 | 0.98 |
| 358_H | 361_K | 1.217 | 0.98 |
| 412_Q | 450_P | 1.211 | 0.98 |
| 187_F | 191_I | 1.209 | 0.98 |
| 172_D | 506_V | 1.203 | 0.98 |
| 336_V | 425_N | 1.193 | 0.98 |
| 363_K | 367_A | 1.192 | 0.98 |
| 157_E | 189_N | 1.174 | 0.98 |
| 127_N | 130_R | 1.171 | 0.98 |
| 422_S | 425_N | 1.169 | 0.97 |
| 261_K | 264_M | 1.168 | 0.97 |
| 339_R | 342_D | 1.161 | 0.97 |
| 371_E | 374_R | 1.160 | 0.97 |
| 326_A | 424_F | 1.155 | 0.97 |
| 193_R | 282_D | 1.151 | 0.97 |
| 387_P | 401_G | 1.147 | 0.97 |
| 259_T | 262_E | 1.146 | 0.97 |
| 128_A | 132_N | 1.135 | 0.97 |
| 155_R | 474_S | 1.133 | 0.97 |
| 44_G | 50_S | 1.129 | 0.97 |
| 344_L | 365_L | 1.124 | 0.97 |
| 216_R | 244_K | 1.120 | 0.96 |
| 164_Y | 178_I | 1.116 | 0.96 |
| 98_Q | 391_C | 1.115 | 0.96 |
| 262_E | 266_S | 1.111 | 0.96 |
| 373_F | 378_P | 1.111 | 0.96 |
| 321_A | 378_P | 1.109 | 0.96 |
| 332_R | 483_L | 1.100 | 0.96 |
| 348_V | 477_H | 1.097 | 0.96 |
| 65_I | 73_F | 1.092 | 0.96 |
| 176_N | 324_E | 1.092 | 0.96 |
| 333_D | 423_E | 1.082 | 0.96 |
| 386_A | 403_T | 1.075 | 0.95 |
| 454_G | 458_C | 1.073 | 0.95 |
| 175_I | 179_S | 1.068 | 0.95 |
| 419_E | 425_N | 1.068 | 0.95 |
| 300_T | 303_Q | 1.065 | 0.95 |
| 161_A | 182_T | 1.060 | 0.95 |
| 265_N | 268_S | 1.060 | 0.95 |
| 158_V | 470_H | 1.060 | 0.95 |
| 67_H | 415_P | 1.059 | 0.95 |
| 331_F | 481_W | 1.052 | 0.95 |
| 187_F | 315_G | 1.049 | 0.94 |
| 154_R | 186_V | 1.048 | 0.94 |
| 463_L | 467_M | 1.048 | 0.94 |
| 104_N | 388_I | 1.046 | 0.94 |
| 340_V | 364_Y | 1.043 | 0.94 |
| 335_K | 339_R | 1.043 | 0.94 |
| 263_R | 266_S | 1.041 | 0.94 |
| 365_L | 368_V | 1.040 | 0.94 |
| 151_H | 155_R | 1.034 | 0.94 |
| 102_F | 387_P | 1.031 | 0.94 |
| 262_E | 265_N | 1.029 | 0.94 |
| 257_E | 301_M | 1.023 | 0.93 |
| 334_Q | 338_K | 1.022 | 0.93 |
| 102_F | 404_I | 1.022 | 0.93 |
| 57_N | 64_K | 1.016 | 0.93 |
| 90_L | 93_V | 1.016 | 0.93 |
| 157_E | 185_N | 1.015 | 0.93 |
| 282_D | 285_Q | 1.014 | 0.93 |
| 93_V | 394_G | 1.014 | 0.93 |
| 326_A | 369_C | 1.013 | 0.93 |
| 146_N | 149_A | 1.012 | 0.93 |
| 38_L | 396_Y | 1.009 | 0.93 |
| 88_A | 414_D | 1.008 | 0.93 |
| 73_F | 86_N | 1.005 | 0.93 |
| 347_I | 361_K | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4r21A | 2 | 0.8096 | 100 | 0.239 | Contact Map |
| 3czhA | 2 | 0.8808 | 100 | 0.252 | Contact Map |
| 2hi4A | 1 | 0.8942 | 100 | 0.253 | Contact Map |
| 3pm0A | 1 | 0.8558 | 100 | 0.253 | Contact Map |
| 3tbgA | 4 | 0.8769 | 100 | 0.254 | Contact Map |
| 3swzA | 4 | 0.8865 | 100 | 0.265 | Contact Map |
| 3nxuA | 2 | 0.8654 | 100 | 0.269 | Contact Map |
| 3k9vA | 2 | 0.8654 | 100 | 0.269 | Contact Map |
| 1r9oA | 1 | 0.8538 | 100 | 0.273 | Contact Map |
| 1po5A | 1 | 0.8731 | 100 | 0.274 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)