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OPENSEQ.org
MHPD - 2-keto-4-pentenoate hydratase
UniProt: P77608 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14274
Length: 269 (259)
Sequences: 1018
Seq/Len: 3.93
MHPD
Paralog alert: 0.33 [within 20: 0.08] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
115_D 223_R 4.195 1.00
54_R 85_D 3.532 1.00
12_D 29_L 3.511 1.00
113_N 131_E 2.969 1.00
188_N 243_D 2.961 1.00
34_N 37_A 2.938 1.00
109_A 136_A 2.917 1.00
115_D 224_T 2.861 1.00
11_A 48_H 2.574 1.00
44_I 47_Q 2.486 1.00
66_P 70_Q 2.441 1.00
115_D 221_P 2.35 1.00
110_L 230_T 2.315 1.00
99_V 163_V 2.271 1.00
239_V 245_F 2.254 1.00
116_L 129_A 2.247 1.00
112_L 222_L 2.078 1.00
57_V 164_Y 1.931 1.00
87_C 164_Y 1.919 1.00
50_V 56_V 1.863 1.00
119_T 213_R 1.839 1.00
114_R 128_N 1.788 0.99
182_A 194_S 1.768 0.99
15_R 19_Q 1.748 0.99
46_V 83_F 1.732 0.99
15_R 18_E 1.709 0.99
49_D 54_R 1.688 0.99
187_R 245_F 1.678 0.99
223_R 226_D 1.675 0.99
2_T 5_T 1.668 0.99
130_I 208_A 1.625 0.99
88_Y 163_V 1.607 0.99
17_A 49_D 1.604 0.99
205_L 209_V 1.579 0.98
88_Y 94_I 1.545 0.98
239_V 259_F 1.518 0.98
18_E 52_Q 1.508 0.98
106_A 247_A 1.492 0.97
111_V 132_W 1.479 0.97
120_D 213_R 1.471 0.97
182_A 196_R 1.459 0.97
11_A 15_R 1.448 0.97
57_V 87_C 1.444 0.97
181_C 249_I 1.432 0.96
182_A 198_S 1.43 0.96
94_I 163_V 1.397 0.96
108_I 233_L 1.395 0.96
93_I 260_S 1.392 0.96
14_R 52_Q 1.375 0.95
57_V 227_I 1.368 0.95
88_Y 95_P 1.358 0.95
43_H 220_E 1.35 0.95
7_E 11_A 1.346 0.94
56_V 81_T 1.344 0.94
78_D 154_T 1.344 0.94
24_A 145_R 1.339 0.94
122_T 125_E 1.332 0.94
54_R 84_A 1.316 0.94
125_E 128_N 1.312 0.93
184_K 194_S 1.305 0.93
15_R 21_E 1.303 0.93
143_R 157_D 1.295 0.93
194_S 199_E 1.292 0.93
104_I 137_L 1.286 0.92
132_W 170_A 1.282 0.92
36_E 217_S 1.274 0.92
186_T 248_H 1.266 0.92
100_L 141_G 1.252 0.91
126_L 208_A 1.25 0.91
165_V 255_V 1.239 0.91
208_A 230_T 1.237 0.90
5_T 9_L 1.233 0.90
47_Q 51_A 1.22 0.90
110_L 208_A 1.219 0.90
184_K 191_E 1.216 0.89
54_R 83_F 1.214 0.89
111_V 227_I 1.207 0.89
193_S 235_P 1.196 0.88
69_Q 210_W 1.19 0.88
68_V 154_T 1.189 0.88
172_R 175_G 1.186 0.88
133_V 178_L 1.167 0.87
99_V 141_G 1.163 0.86
90_D 256_A 1.15 0.86
111_V 134_L 1.149 0.85
191_E 194_S 1.146 0.85
200_C 232_A 1.141 0.85
134_L 170_A 1.139 0.85
118_A 121_I 1.132 0.84
233_L 249_I 1.132 0.84
246_E 256_A 1.128 0.84
76_Q 214_K 1.126 0.84
68_V 236_M 1.122 0.84
88_Y 92_E 1.118 0.83
68_V 140_V 1.105 0.82
9_L 12_D 1.097 0.82
16_A 23_I 1.094 0.81
14_R 45_N 1.079 0.80
142_S 160_S 1.077 0.80
108_I 249_I 1.075 0.80
184_K 248_H 1.073 0.80
117_P 221_P 1.072 0.80
106_A 197_G 1.068 0.79
14_R 48_H 1.068 0.79
7_E 44_I 1.065 0.79
186_T 246_E 1.062 0.79
246_E 254_S 1.059 0.78
159_A 162_G 1.054 0.78
70_Q 73_G 1.048 0.77
178_L 197_G 1.047 0.77
111_V 131_E 1.043 0.77
5_T 8_Q 1.035 0.76
101_Q 147_W 1.034 0.76
125_E 130_I 1.021 0.75
200_C 233_L 1.021 0.75
112_L 224_T 1.014 0.74
14_R 18_E 1.014 0.74
59_R 226_D 1.011 0.74
185_M 234_G 1.007 0.73
123_F 206_N 1.007 0.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2wqtA2011000.232Contact Map0.724
2eb4A50.96651000.252Contact Map0.789
1sawA20.81411000.6Contact Map0.549
3s52A40.79551000.61Contact Map0.576
4maqA20.86621000.626Contact Map0.603
3l53A60.79551000.627Contact Map0.617
1gttA40.91451000.639Contact Map0.698
1wzoA40.88851000.645Contact Map0.711
1nkqA60.80671000.647Contact Map0.595
3r6oA10.940599.90.671Contact Map0.58
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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