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OPENSEQ.org
MHPA - 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
UniProt: P77397 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG20273
Length: 554 (531)
Sequences: 711
Seq/Len: 1.34
MHPA
Paralog alert: 0.15 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
286_R 336_D 4.901 1.00
30_M 171_V 4.63 1.00
147_Q 150_E 3.502 1.00
19_A 153_L 3.39 1.00
444_V 512_V 3.322 1.00
173_C 293_A 3.196 1.00
144_F 170_L 3.039 1.00
171_V 293_A 2.914 1.00
141_L 153_L 2.889 1.00
147_Q 166_K 2.706 1.00
32_N 126_G 2.703 1.00
339_Q 343_R 2.7 1.00
494_D 497_G 2.696 1.00
317_N 321_K 2.686 1.00
20_I 171_V 2.658 1.00
39_I 326_I 2.649 1.00
17_Q 168_Q 2.558 1.00
32_N 130_F 2.504 1.00
21_A 180_V 2.465 1.00
126_G 130_F 2.406 0.99
330_A 334_L 2.359 0.99
150_E 166_K 2.3 0.99
30_M 319_A 2.251 0.99
186_V 282_A 2.171 0.99
23_A 28_L 2.168 0.99
169_W 326_I 2.161 0.99
44_V 155_L 2.132 0.98
286_R 332_D 2.129 0.98
147_Q 152_T 2.128 0.98
444_V 510_L 2.109 0.98
286_R 291_L 2.08 0.98
282_A 292_L 2.062 0.98
246_Q 249_E 2.061 0.98
152_T 166_K 2.051 0.98
20_I 41_V 2.032 0.98
46_K 140_E 1.981 0.97
35_G 133_V 1.974 0.97
67_M 312_M 1.918 0.97
126_G 129_R 1.896 0.96
21_A 170_L 1.881 0.96
39_I 323_A 1.872 0.96
188_F 280_R 1.855 0.96
151_V 170_L 1.823 0.95
42_L 136_L 1.799 0.95
127_V 133_V 1.791 0.94
186_V 292_L 1.769 0.94
15_S 168_Q 1.765 0.94
17_Q 40_D 1.757 0.94
143_A 183_T 1.698 0.92
141_L 144_F 1.695 0.92
42_L 165_V 1.693 0.92
277_H 303_W 1.691 0.92
124_L 137_F 1.658 0.91
15_S 166_K 1.652 0.91
63_A 312_M 1.644 0.90
20_I 30_M 1.64 0.90
41_V 132_N 1.631 0.90
249_E 252_N 1.617 0.89
286_R 335_L 1.612 0.89
140_E 156_K 1.582 0.88
18_V 34_L 1.579 0.88
19_A 44_V 1.559 0.87
184_L 290_V 1.55 0.86
26_V 311_G 1.544 0.86
44_V 141_L 1.519 0.85
340_Q 403_A 1.503 0.84
16_V 42_L 1.5 0.84
291_L 336_D 1.493 0.83
33_Y 70_V 1.492 0.83
193_A 277_H 1.479 0.83
186_V 287_I 1.477 0.83
284_R 287_I 1.47 0.82
62_E 65_R 1.47 0.82
17_Q 39_I 1.461 0.82
30_M 293_A 1.455 0.81
171_V 319_A 1.453 0.81
308_Y 312_M 1.444 0.81
198_I 277_H 1.434 0.80
192_T 278_N 1.423 0.79
324_L 330_A 1.413 0.79
321_K 337_T 1.403 0.78
65_R 313_R 1.402 0.78
26_V 293_A 1.391 0.77
306_Q 349_M 1.39 0.77
323_A 327_Q 1.388 0.77
72_L 126_G 1.386 0.77
172_A 184_L 1.366 0.75
189_E 283_Q 1.358 0.75
324_L 329_K 1.356 0.74
50_L 124_L 1.351 0.74
256_L 305_G 1.329 0.72
33_Y 319_A 1.32 0.72
198_I 353_S 1.319 0.71
186_V 339_Q 1.318 0.71
54_P 271_R 1.308 0.71
179_F 183_T 1.3 0.70
75_D 129_R 1.285 0.69
253_M 272_Q 1.284 0.69
325_V 336_D 1.276 0.68
187_P 283_Q 1.276 0.68
62_E 313_R 1.275 0.68
190_G 280_R 1.27 0.67
313_R 345_H 1.267 0.67
184_L 287_I 1.266 0.67
289_R 322_L 1.26 0.66
343_R 347_K 1.256 0.66
325_V 337_T 1.252 0.66
168_Q 171_V 1.252 0.66
252_N 255_K 1.252 0.66
167_A 185_N 1.249 0.65
37_M 323_A 1.248 0.65
447_W 499_L 1.231 0.64
203_A 268_E 1.231 0.64
146_Q 290_V 1.23 0.64
300_M 353_S 1.226 0.63
79_H 122_V 1.225 0.63
169_W 332_D 1.22 0.63
216_C 357_G 1.214 0.62
32_N 70_V 1.21 0.62
9_Q 13_N 1.207 0.62
30_M 315_A 1.204 0.61
24_G 27_G 1.203 0.61
142_E 154_H 1.2 0.61
22_G 27_G 1.196 0.60
139_R 155_L 1.195 0.60
29_M 312_M 1.186 0.60
62_E 345_H 1.184 0.59
142_E 183_T 1.183 0.59
231_V 270_I 1.179 0.59
44_V 128_S 1.177 0.59
20_I 45_E 1.175 0.58
186_V 202_I 1.17 0.58
345_H 397_P 1.169 0.58
9_Q 12_V 1.163 0.57
172_A 180_V 1.158 0.57
324_L 442_F 1.151 0.56
146_Q 287_I 1.144 0.55
243_T 246_Q 1.142 0.55
67_M 76_V 1.142 0.55
141_L 155_L 1.141 0.55
136_L 163_E 1.141 0.55
67_M 119_V 1.138 0.55
242_E 301_P 1.136 0.55
31_A 43_V 1.135 0.54
74_D 78_P 1.133 0.54
405_M 408_G 1.132 0.54
233_R 275_Y 1.128 0.54
281_L 347_K 1.121 0.53
148_D 166_K 1.117 0.53
72_L 123_M 1.115 0.53
21_A 167_A 1.114 0.52
169_W 335_L 1.113 0.52
20_I 471_I 1.111 0.52
22_G 25_P 1.107 0.52
10_P 13_N 1.106 0.52
193_A 198_I 1.104 0.51
285_F 309_N 1.103 0.51
198_I 303_W 1.102 0.51
23_A 309_N 1.102 0.51
174_D 178_S 1.1 0.51
281_L 343_R 1.099 0.51
53_Y 277_H 1.098 0.51
172_A 292_L 1.091 0.50
238_V 253_M 1.091 0.50
54_P 235_E 1.088 0.50
19_A 167_A 1.088 0.50
502_W 511_V 1.088 0.50
30_M 221_P 1.088 0.50
58_G 227_L 1.087 0.50
68_Q 436_N 1.086 0.50
62_E 357_G 1.086 0.50
275_Y 279_A 1.084 0.49
197_W 235_E 1.084 0.49
428_N 465_A 1.083 0.49
300_M 349_M 1.082 0.49
150_E 170_L 1.08 0.49
310_S 346_A 1.076 0.49
145_S 154_H 1.073 0.48
167_A 170_L 1.071 0.48
35_G 283_Q 1.071 0.48
285_F 346_A 1.069 0.48
512_V 518_F 1.068 0.48
148_D 152_T 1.065 0.47
26_V 315_A 1.062 0.47
45_E 88_F 1.061 0.47
153_L 167_A 1.058 0.47
153_L 170_L 1.058 0.47
45_E 384_V 1.052 0.46
291_L 344_D 1.05 0.46
326_I 513_M 1.05 0.46
60_D 357_G 1.05 0.46
45_E 188_F 1.049 0.46
58_G 306_Q 1.048 0.46
366_W 513_M 1.047 0.46
65_R 357_G 1.047 0.46
43_V 134_R 1.046 0.46
188_F 299_I 1.046 0.46
75_D 125_E 1.045 0.45
142_E 170_L 1.044 0.45
60_D 313_R 1.043 0.45
68_Q 438_I 1.041 0.45
302_V 305_G 1.04 0.45
494_D 503_F 1.039 0.45
225_A 236_F 1.039 0.45
352_L 517_R 1.034 0.44
60_D 65_R 1.027 0.44
34_L 39_I 1.024 0.43
29_M 66_T 1.023 0.43
68_Q 421_Q 1.022 0.43
59_I 308_Y 1.02 0.43
323_A 326_I 1.015 0.43
190_G 223_V 1.015 0.43
26_V 308_Y 1.013 0.42
16_V 167_A 1.012 0.42
280_R 299_I 1.012 0.42
57_I 235_E 1.011 0.42
281_L 349_M 1.007 0.42
531_T 534_K 1.007 0.42
62_E 80_T 1.005 0.42
277_H 302_V 1.005 0.42
190_G 228_P 1.004 0.41
68_Q 435_D 1.003 0.41
221_P 225_A 1.002 0.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2qa2A10.89531000.445Contact Map0.644
2qa1A10.89711000.45Contact Map0.661
4k2xA20.90431000.458Contact Map0.642
3ihgA30.89351000.46Contact Map0.634
2dkhA10.96031000.468Contact Map0.623
4k5sA10.92241000.469Contact Map0.657
1pn0A40.96931000.469Contact Map0.599
3fmwA30.92961000.472Contact Map0.58
2r0cA10.90071000.478Contact Map0.602
4n9xA10.7131000.641Contact Map0.54
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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