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OPENSEQ.org
PRPD - 2-methylcitrate dehydratase
UniProt: P77243 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13603
Length: 483 (467)
Sequences: 1124
Seq/Len: 2.41
PRPD
Paralog alert: 0.19 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
184_A 194_I 4.605 1.00
318_E 412_T 3.879 1.00
75_R 125_D 3.528 1.00
197_A 239_A 3.266 1.00
190_T 193_E 3.166 1.00
319_K 384_N 3.09 1.00
21_D 191_R 2.853 1.00
46_G 443_F 2.546 1.00
318_E 410_E 2.529 1.00
147_A 186_M 2.464 1.00
300_A 352_V 2.4 1.00
140_T 143_Q 2.301 1.00
37_A 149_I 2.263 1.00
411_F 417_F 2.249 1.00
301_M 355_P 2.239 1.00
42_L 460_L 2.224 1.00
358_F 365_D 2.211 1.00
426_I 438_K 2.205 1.00
232_A 236_A 2.175 1.00
39_Y 436_I 2.172 1.00
321_T 386_F 2.128 1.00
316_D 412_T 2.09 1.00
164_F 173_L 2.086 1.00
123_T 233_V 2.07 1.00
408_T 418_E 2.065 1.00
127_L 139_L 2.02 0.99
321_T 410_E 1.942 0.99
42_L 463_S 1.941 0.99
34_Y 146_T 1.939 0.99
96_I 115_D 1.937 0.99
74_V 88_A 1.92 0.99
200_L 220_R 1.915 0.99
319_K 410_E 1.896 0.99
26_Y 150_K 1.889 0.99
187_L 237_L 1.888 0.99
44_T 96_I 1.888 0.99
444_K 457_Q 1.883 0.99
457_Q 461_E 1.825 0.99
465_D 468_R 1.817 0.98
181_A 198_V 1.803 0.98
477_Y 481_Y 1.776 0.98
323_R 388_D 1.773 0.98
323_R 406_A 1.769 0.98
289_A 293_S 1.754 0.98
440_V 464_L 1.751 0.98
360_R 365_D 1.747 0.98
180_T 232_A 1.746 0.98
37_A 145_L 1.734 0.98
41_L 145_L 1.719 0.98
12_F 195_L 1.706 0.97
146_T 185_E 1.705 0.97
120_I 183_V 1.698 0.97
378_A 382_K 1.694 0.97
34_Y 38_H 1.689 0.97
184_A 189_L 1.688 0.97
155_Q 178_A 1.664 0.97
85_P 126_W 1.657 0.97
435_G 438_K 1.655 0.97
313_T 316_D 1.635 0.96
358_F 373_D 1.632 0.96
391_F 421_V 1.631 0.96
35_D 436_I 1.625 0.96
410_E 416_R 1.625 0.96
41_L 148_M 1.618 0.96
24_M 194_I 1.618 0.96
374_K 389_P 1.582 0.95
299_A 422_V 1.573 0.95
321_T 384_N 1.569 0.95
297_V 355_P 1.568 0.95
189_L 239_A 1.56 0.95
200_L 239_A 1.556 0.95
57_C 95_M 1.555 0.95
315_A 375_R 1.545 0.95
342_A 345_D 1.53 0.94
180_T 197_A 1.526 0.94
406_A 421_V 1.521 0.94
304_Y 359_G 1.503 0.94
321_T 408_T 1.502 0.94
377_D 380_R 1.484 0.93
117_L 148_M 1.47 0.93
196_N 239_A 1.469 0.93
158_I 177_V 1.466 0.92
411_F 415_T 1.462 0.92
333_D 349_Q 1.444 0.92
449_R 452_P 1.433 0.91
466_R 470_E 1.431 0.91
90_F 93_G 1.426 0.91
189_L 240_K 1.424 0.91
322_I 348_I 1.42 0.91
350_Y 380_R 1.413 0.91
34_Y 145_L 1.402 0.90
322_I 352_V 1.399 0.90
227_D 231_R 1.364 0.88
222_S 345_D 1.36 0.88
303_L 409_L 1.353 0.88
85_P 89_A 1.352 0.88
33_A 279_E 1.349 0.88
120_I 182_V 1.348 0.88
97_R 224_A 1.338 0.87
87_Q 91_N 1.338 0.87
315_A 412_T 1.337 0.87
177_V 198_V 1.336 0.87
23_V 181_A 1.329 0.87
444_K 456_Q 1.326 0.86
16_I 195_L 1.326 0.86
105_W 109_E 1.322 0.86
30_S 33_A 1.317 0.86
141_M 466_R 1.316 0.86
76_V 83_L 1.315 0.86
123_T 127_L 1.309 0.85
187_L 240_K 1.296 0.85
105_W 277_V 1.288 0.84
20_V 195_L 1.276 0.83
123_T 183_V 1.276 0.83
34_Y 149_I 1.269 0.83
104_T 282_L 1.266 0.83
35_D 39_Y 1.257 0.82
88_A 92_I 1.249 0.82
124_A 144_V 1.247 0.81
306_Q 419_E 1.246 0.81
104_T 291_F 1.244 0.81
98_W 222_S 1.244 0.81
23_V 198_V 1.241 0.81
109_E 159_A 1.237 0.81
97_R 170_D 1.234 0.81
172_V 207_S 1.234 0.81
314_A 411_F 1.233 0.80
119_G 179_S 1.228 0.80
51_A 95_M 1.227 0.80
187_L 236_A 1.226 0.80
348_I 385_C 1.222 0.80
170_D 224_A 1.219 0.79
203_V 208_L 1.213 0.79
143_Q 185_E 1.212 0.79
14_R 18_D 1.201 0.78
189_L 197_A 1.198 0.78
97_R 102_N 1.188 0.77
288_P 361_L 1.185 0.77
203_V 224_A 1.185 0.77
357_L 375_R 1.18 0.76
123_T 187_L 1.179 0.76
38_H 467_A 1.178 0.76
290_E 346_H 1.177 0.76
154_I 181_A 1.175 0.76
46_G 49_L 1.17 0.75
123_T 186_M 1.166 0.75
314_A 357_L 1.165 0.75
362_T 365_D 1.159 0.74
24_M 191_R 1.155 0.74
196_N 244_M 1.151 0.74
323_R 408_T 1.151 0.74
453_T 457_Q 1.144 0.73
100_D 114_S 1.139 0.73
202_W 249_A 1.138 0.72
353_A 379_L 1.136 0.72
104_T 403_I 1.133 0.72
358_F 370_V 1.13 0.72
452_P 456_Q 1.127 0.71
297_V 351_M 1.127 0.71
189_L 194_I 1.126 0.71
58_K 62_G 1.123 0.71
300_A 356_L 1.119 0.71
77_P 92_I 1.118 0.71
32_V 279_E 1.117 0.70
62_G 241_T 1.115 0.70
22_Y 276_Y 1.11 0.70
34_Y 142_K 1.109 0.70
93_G 119_G 1.107 0.69
97_R 171_H 1.107 0.69
77_P 121_L 1.107 0.69
120_I 144_V 1.106 0.69
70_V 73_G 1.1 0.69
97_R 345_D 1.098 0.69
293_S 351_M 1.098 0.69
238_M 243_E 1.095 0.68
119_G 233_V 1.094 0.68
218_G 222_S 1.079 0.67
54_Y 57_C 1.077 0.66
461_E 465_D 1.075 0.66
289_A 366_Y 1.072 0.66
119_G 183_V 1.069 0.66
171_H 224_A 1.069 0.66
323_R 386_F 1.061 0.65
51_A 98_W 1.061 0.65
97_R 115_D 1.059 0.65
89_A 93_G 1.058 0.64
114_S 168_G 1.054 0.64
227_D 230_S 1.05 0.64
145_L 149_I 1.049 0.63
288_P 365_D 1.049 0.63
444_K 460_L 1.046 0.63
179_S 232_A 1.045 0.63
307_M 407_I 1.045 0.63
176_K 206_Q 1.04 0.62
90_F 94_A 1.039 0.62
317_I 409_L 1.037 0.62
8_I 476_E 1.036 0.62
102_N 170_D 1.034 0.62
140_T 320_V 1.033 0.62
291_F 294_Q 1.029 0.61
195_L 239_A 1.027 0.61
175_V 228_A 1.023 0.61
458_R 467_A 1.021 0.60
193_E 196_N 1.02 0.60
288_P 446_N 1.019 0.60
30_S 276_Y 1.019 0.60
114_S 152_H 1.017 0.60
23_V 194_I 1.016 0.60
40_C 283_F 1.014 0.60
282_L 291_F 1.014 0.60
277_V 280_N 1.013 0.59
75_R 82_Q 1.011 0.59
296_A 348_I 1.006 0.59
32_V 35_D 1.004 0.58
9_R 12_F 1.002 0.58
219_T 243_E 1.002 0.58
74_V 125_D 1 0.58
170_D 338_L 1 0.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1szqA20.99591000.098Contact Map0.783
2hp0A10.92551000.121Contact Map0.694
4iggA20.17616.50.977Contact Map0.147
1h0hA20.190513.90.978Contact Map0.146
2wujA20.103511.90.978Contact Map0.378
3r88A20.144911.60.979Contact Map0.204
4giuA20.14711.50.979Contact Map0.229
4eukB10.113910.80.979Contact Map0.408
1ti6A60.18019.10.98Contact Map0.346
3ml1A10.20088.90.98Contact Map0.291
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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