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OPENSEQ.org
NFI - Endonuclease V
UniProt: P68739 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11915
Length: 223 (214)
Sequences: 496
Seq/Len: 2.32
NFI
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
21_D 181_S 3.379 1.00
53_K 56_S 3.017 1.00
113_L 117_S 2.766 1.00
139_F 173_F 2.609 1.00
112_R 157_E 2.568 1.00
206_D 210_S 2.352 1.00
101_F 202_T 2.267 1.00
19_R 176_T 2.097 1.00
105_H 108_S 2.033 0.99
165_S 172_L 2.028 0.99
161_W 186_L 1.991 0.99
90_W 123_V 1.959 0.99
176_T 182_V 1.855 0.99
34_A 86_L 1.842 0.99
17_V 121_L 1.831 0.98
181_S 184_S 1.772 0.98
143_S 148_A 1.744 0.98
145_E 167_A 1.743 0.98
21_D 184_S 1.695 0.97
107_I 117_S 1.672 0.97
158_Q 161_W 1.644 0.96
124_D 179_R 1.608 0.96
107_I 176_T 1.584 0.95
62_Y 208_V 1.582 0.95
206_D 209_A 1.574 0.95
11_I 14_A 1.567 0.95
128_I 189_V 1.525 0.94
99_L 188_W 1.5 0.93
61_E 94_S 1.484 0.93
171_P 203_R 1.471 0.92
38_F 69_T 1.458 0.92
101_F 130_V 1.451 0.91
50_V 59_L 1.435 0.91
134_R 162_V 1.417 0.90
52_L 57_L 1.388 0.89
55_P 192_C 1.383 0.89
200_E 203_R 1.376 0.88
102_V 201_P 1.347 0.87
165_S 193_M 1.347 0.87
102_V 119_F 1.344 0.87
48_A 209_A 1.344 0.87
112_R 154_D 1.337 0.86
37_G 209_A 1.329 0.86
30_L 51_L 1.324 0.86
32_A 51_L 1.317 0.85
83_Y 119_F 1.316 0.85
13_L 113_L 1.293 0.84
165_S 190_Q 1.292 0.84
22_R 179_R 1.266 0.82
30_L 60_V 1.264 0.82
171_P 197_R 1.262 0.82
46_R 210_S 1.254 0.81
88_A 91_E 1.249 0.81
7_R 77_F 1.242 0.80
149_L 161_W 1.227 0.79
189_V 197_R 1.22 0.79
45_T 85_A 1.206 0.77
163_W 193_M 1.199 0.77
71_M 206_D 1.198 0.77
197_R 203_R 1.184 0.76
188_W 201_P 1.178 0.75
108_S 175_A 1.177 0.75
37_G 46_R 1.175 0.75
152_L 162_V 1.165 0.74
154_D 159_L 1.165 0.74
67_I 85_A 1.159 0.73
60_V 189_V 1.158 0.73
142_L 150_A 1.15 0.73
105_H 137_G 1.143 0.72
47_A 119_F 1.14 0.72
32_A 49_M 1.135 0.71
190_Q 193_M 1.127 0.70
37_G 210_S 1.124 0.70
31_I 127_T 1.124 0.70
36_V 86_L 1.12 0.70
50_V 204_W 1.118 0.69
90_W 100_V 1.112 0.69
26_D 191_R 1.101 0.68
89_A 93_L 1.099 0.68
43_E 64_V 1.098 0.67
101_F 189_V 1.097 0.67
9_Q 77_F 1.094 0.67
100_V 127_T 1.093 0.67
108_S 176_T 1.093 0.67
31_I 34_A 1.091 0.67
146_P 190_Q 1.091 0.67
196_Y 203_R 1.088 0.66
36_V 115_V 1.081 0.66
105_H 133_K 1.07 0.65
52_L 202_T 1.066 0.64
103_D 132_K 1.062 0.64
115_V 135_L 1.061 0.64
79_S 105_H 1.055 0.63
30_L 95_Q 1.049 0.62
90_W 119_F 1.048 0.62
21_D 179_R 1.047 0.62
76_G 84_P 1.046 0.62
34_A 119_F 1.046 0.62
145_E 148_A 1.042 0.61
84_P 87_L 1.04 0.61
161_W 182_V 1.036 0.61
28_P 100_V 1.029 0.60
101_F 174_I 1.027 0.60
13_L 16_S 1.024 0.59
53_K 58_E 1.024 0.59
8_A 16_S 1.024 0.59
144_S 148_A 1.02 0.59
166_K 169_C 1.019 0.59
33_G 202_T 1.019 0.59
77_F 108_S 1.017 0.59
67_I 88_A 1.013 0.58
100_V 125_V 1.011 0.58
127_T 159_L 1.007 0.58
86_L 102_V 1.005 0.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ga2A111000.149Contact Map0.644
2w36A20.95961000.258Contact Map0.691
3gocA211000.291Contact Map0.7
2qh9A20.80271000.47Contact Map0.381
2ygkA20.977696.60.913Contact Map0.462
2nrrA10.677195.80.919Contact Map0.46
3c65A10.8795.20.922Contact Map0.49
2nrtA10.86194.90.924Contact Map0.413
2w42A20.955276.20.945Contact Map0.398
2yhbA10.9283410.956Contact Map0.277
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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