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OPENSEQ.org
KUP - Low affinity potassium transport system protein kup
UniProt: P63183 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11541
Length: 622 (618)
Sequences: 893
Seq/Len: 1.44
KUP
Paralog alert: 0.12 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
281_A 289_L 3.831 1.00
61_I 252_L 3.448 1.00
73_M 235_M 3.447 1.00
53_L 194_V 3.281 1.00
50_F 194_V 2.804 1.00
65_S 248_F 2.8 1.00
57_F 198_L 2.755 1.00
114_Y 312_T 2.67 1.00
181_A 254_S 2.595 1.00
181_A 257_L 2.572 1.00
129_I 146_L 2.555 1.00
118_V 353_V 2.498 1.00
152_T 347_Y 2.423 1.00
129_I 150_V 2.351 0.99
494_N 498_N 2.325 0.99
256_T 260_F 2.225 0.99
240_K 244_R 2.217 0.99
419_L 427_L 2.212 0.99
201_M 205_H 2.166 0.99
57_F 260_F 2.115 0.99
183_L 281_A 2.099 0.99
198_L 260_F 2.052 0.98
278_F 290_L 2.019 0.98
70_T 244_R 2.007 0.98
57_F 61_I 1.976 0.98
538_A 548_V 1.958 0.97
53_L 263_G 1.953 0.97
124_S 297_T 1.915 0.97
53_L 260_F 1.912 0.97
112_F 373_G 1.898 0.97
222_V 411_L 1.873 0.96
306_S 423_G 1.862 0.96
174_L 250_V 1.856 0.96
241_F 244_R 1.846 0.96
576_L 593_L 1.84 0.96
127_S 297_T 1.831 0.96
183_L 289_L 1.818 0.96
66_I 403_A 1.811 0.95
179_I 289_L 1.796 0.95
412_F 416_L 1.793 0.95
62_F 66_I 1.75 0.94
59_L 224_S 1.746 0.94
222_V 372_T 1.731 0.94
117_V 304_V 1.729 0.94
383_T 397_V 1.705 0.93
513_E 539_S 1.697 0.93
207_F 214_S 1.687 0.93
287_I 290_L 1.672 0.92
55_L 203_A 1.667 0.92
157_I 168_L 1.639 0.91
57_F 256_T 1.636 0.91
206_F 217_A 1.636 0.91
525_Q 539_S 1.622 0.91
196_H 202_W 1.612 0.90
83_I 109_G 1.597 0.90
193_E 196_H 1.573 0.89
113_F 350_V 1.549 0.88
187_S 280_L 1.546 0.88
146_L 149_I 1.537 0.87
507_L 533_F 1.536 0.87
564_M 569_F 1.531 0.87
358_E 362_N 1.527 0.87
60_L 109_G 1.52 0.86
118_V 349_A 1.514 0.86
50_F 57_F 1.501 0.85
21_Y 73_M 1.478 0.84
53_L 256_T 1.468 0.84
517_Y 544_E 1.452 0.83
181_A 427_L 1.436 0.82
353_V 425_L 1.433 0.82
178_L 182_G 1.432 0.82
187_S 190_A 1.417 0.81
416_L 419_L 1.417 0.81
194_V 263_G 1.415 0.80
345_M 436_V 1.412 0.80
526_I 536_V 1.41 0.80
113_F 117_V 1.408 0.80
18_G 26_T 1.405 0.80
36_L 48_A 1.383 0.78
72_V 378_T 1.383 0.78
21_Y 378_T 1.371 0.77
383_T 401_L 1.37 0.77
59_L 411_L 1.366 0.77
147_S 354_I 1.366 0.77
527_E 535_R 1.362 0.77
586_R 590_K 1.355 0.76
182_G 185_L 1.355 0.76
118_V 481_S 1.35 0.76
123_I 306_S 1.346 0.76
150_V 459_E 1.333 0.75
569_F 608_I 1.331 0.74
83_I 437_M 1.322 0.74
35_C 52_F 1.32 0.74
71_F 382_S 1.319 0.74
17_I 174_L 1.317 0.73
436_V 595_L 1.311 0.73
123_I 278_F 1.309 0.73
104_I 436_V 1.308 0.73
66_I 70_T 1.307 0.73
347_Y 351_V 1.305 0.72
216_I 417_D 1.295 0.72
77_N 542_W 1.294 0.71
75_A 219_G 1.287 0.71
69_L 510_L 1.287 0.71
135_V 287_I 1.28 0.70
379_S 400_I 1.278 0.70
204_V 348_V 1.278 0.70
316_V 326_I 1.277 0.70
392_W 396_F 1.275 0.70
23_D 27_S 1.275 0.70
452_H 620_V 1.272 0.70
286_L 290_L 1.271 0.70
57_F 202_W 1.259 0.68
549_E 569_F 1.254 0.68
129_I 144_V 1.253 0.68
185_L 189_I 1.246 0.67
136_A 139_L 1.232 0.66
265_L 272_A 1.224 0.65
148_I 351_V 1.223 0.65
403_A 407_V 1.223 0.65
179_I 292_I 1.218 0.65
177_F 315_A 1.217 0.65
594_L 598_N 1.216 0.65
150_V 298_V 1.213 0.64
494_N 570_F 1.207 0.64
108_I 298_V 1.207 0.64
511_R 539_S 1.207 0.64
71_F 419_L 1.206 0.64
218_L 507_L 1.205 0.64
584_Y 588_R 1.204 0.63
320_Y 441_K 1.203 0.63
246_A 426_P 1.202 0.63
252_L 456_N 1.201 0.63
116_E 228_V 1.201 0.63
34_E 361_S 1.198 0.63
126_M 147_S 1.193 0.62
518_V 544_E 1.192 0.62
178_L 395_Y 1.188 0.62
55_L 411_L 1.188 0.62
72_V 382_S 1.187 0.62
281_A 285_A 1.186 0.62
214_S 414_A 1.186 0.62
50_F 256_T 1.185 0.62
85_T 320_Y 1.184 0.62
117_V 350_V 1.183 0.61
47_D 175_T 1.178 0.61
359_H 362_N 1.176 0.61
424_W 444_R 1.174 0.61
394_K 398_A 1.171 0.60
254_S 414_A 1.17 0.60
25_G 177_F 1.17 0.60
71_F 404_F 1.167 0.60
113_F 304_V 1.165 0.60
49_V 369_I 1.164 0.60
231_L 247_W 1.158 0.59
222_V 415_N 1.157 0.59
342_V 351_V 1.155 0.59
306_S 411_L 1.154 0.59
310_S 370_A 1.149 0.58
59_L 225_I 1.144 0.58
74_R 240_K 1.143 0.57
46_R 196_H 1.141 0.57
187_S 268_K 1.136 0.57
519_H 544_E 1.135 0.57
585_L 588_R 1.133 0.56
204_V 402_I 1.132 0.56
90_A 385_V 1.131 0.56
461_M 608_I 1.126 0.56
306_S 508_L 1.126 0.56
18_G 23_D 1.122 0.55
465_L 507_L 1.122 0.55
365_A 421_S 1.121 0.55
36_L 40_F 1.12 0.55
239_G 360_S 1.12 0.55
165_V 569_F 1.119 0.55
583_W 589_G 1.119 0.55
246_A 313_R 1.115 0.55
555_C 562_C 1.111 0.54
217_A 222_V 1.109 0.54
135_V 207_F 1.109 0.54
31_T 235_M 1.109 0.54
78_A 514_D 1.106 0.54
134_I 424_W 1.104 0.54
14_L 170_A 1.104 0.54
207_F 307_G 1.102 0.53
323_P 481_S 1.102 0.53
191_N 268_K 1.1 0.53
130_E 516_P 1.1 0.53
475_G 488_P 1.097 0.53
117_V 308_V 1.095 0.53
64_V 228_V 1.094 0.53
177_F 299_I 1.094 0.53
45_E 48_A 1.091 0.52
151_L 354_I 1.09 0.52
491_L 507_L 1.086 0.52
75_A 390_W 1.085 0.52
278_F 287_I 1.083 0.51
199_N 292_I 1.083 0.51
23_D 26_T 1.078 0.51
29_L 67_K 1.076 0.51
350_V 494_N 1.073 0.50
494_N 500_V 1.072 0.50
153_L 283_D 1.072 0.50
383_T 387_R 1.071 0.50
115_G 237_H 1.065 0.50
113_F 308_V 1.063 0.49
135_V 286_L 1.061 0.49
75_A 89_L 1.06 0.49
306_S 350_V 1.06 0.49
202_W 260_F 1.058 0.49
610_P 619_Q 1.058 0.49
21_Y 560_L 1.057 0.49
76_D 240_K 1.053 0.48
31_T 35_C 1.053 0.48
125_V 151_L 1.052 0.48
515_A 595_L 1.05 0.48
166_G 419_L 1.048 0.48
475_G 502_H 1.048 0.48
177_F 300_A 1.048 0.48
24_I 307_G 1.047 0.48
405_L 409_I 1.047 0.48
458_L 487_I 1.046 0.48
188_I 383_T 1.046 0.48
352_I 356_S 1.046 0.48
71_F 109_G 1.043 0.47
63_V 408_D 1.041 0.47
281_A 284_W 1.04 0.47
351_V 355_V 1.04 0.47
32_L 266_L 1.039 0.47
442_S 445_F 1.038 0.47
576_L 601_R 1.038 0.47
117_V 309_F 1.036 0.47
97_R 100_S 1.032 0.46
373_G 412_F 1.032 0.46
165_V 275_N 1.032 0.46
436_V 474_P 1.031 0.46
425_L 429_L 1.03 0.46
562_C 565_M 1.03 0.46
108_I 112_F 1.029 0.46
583_W 622_I 1.028 0.46
98_T 107_L 1.027 0.46
36_L 588_R 1.026 0.46
292_I 526_I 1.026 0.46
83_I 374_T 1.026 0.46
461_M 595_L 1.025 0.45
108_I 377_L 1.023 0.45
109_G 437_M 1.022 0.45
220_A 490_A 1.022 0.45
522_R 539_S 1.02 0.45
62_F 400_I 1.02 0.45
48_A 210_Y 1.019 0.45
29_L 542_W 1.019 0.45
295_L 298_V 1.018 0.45
393_N 396_F 1.017 0.45
24_I 367_Y 1.014 0.44
21_Y 512_T 1.012 0.44
255_L 367_Y 1.01 0.44
109_G 117_V 1.009 0.44
166_G 412_F 1.009 0.44
58_W 503_E 1.008 0.44
578_L 604_D 1.008 0.44
58_W 76_D 1.008 0.44
234_D 237_H 1.007 0.44
464_S 611_N 1.006 0.44
73_M 180_L 1.005 0.43
380_I 383_T 1.005 0.43
493_H 565_M 1.002 0.43
327_I 340_P 1.002 0.43
243_I 414_A 1.002 0.43
87_M 608_I 1.002 0.43
267_L 309_F 1.001 0.43
178_L 432_V 1.001 0.43
77_N 318_L 1.001 0.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3giaA10.670497.30.969Contact Map0.302
3l1lA10.670497.10.97Contact Map0.206
4djkA20.707496.40.972Contact Map0.275
2rddB10.05959.20.99Contact Map0.14
3hlzA20.12866.20.99Contact Map0.175
3iezA20.17364.80.991Contact Map0.26
1h95A10.08044.50.991Contact Map0.111
1x0hA10.17043.60.991Contact Map0.15
2lssA10.0743.40.992Contact Map0.128
3h6jA10.22513.30.992Contact Map0.122
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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