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OPENSEQ.org
ISPH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProt: P62623 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11081
Length: 316 (309)
Sequences: 1385
Seq/Len: 4.48
ISPH
Paralog alert: 0.05 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
82_N 86_S 3.372 1.00
216_V 259_V 3.354 1.00
2_Q 261_C 3.243 1.00
159_E 187_K 3.062 1.00
114_E 160_K 2.982 1.00
248_A 277_N 2.732 1.00
219_V 265_T 2.714 1.00
47_D 50_R 2.689 1.00
115_E 142_Y 2.589 1.00
150_V 180_A 2.552 1.00
253_E 285_L 2.482 1.00
218_L 245_I 2.21 1.00
147_P 176_D 2.172 1.00
243_F 255_W 2.097 1.00
4_L 213_Q 2.061 1.00
281_R 285_L 2.038 1.00
251_I 278_V 2.022 1.00
145_E 149_D 2.018 1.00
294_E 297_E 1.989 1.00
232_A 236_Q 1.986 1.00
147_P 177_V 1.914 1.00
83_E 86_S 1.893 1.00
115_E 156_K 1.889 1.00
44_Y 247_D 1.865 1.00
241_R 259_V 1.844 1.00
201_T 205_E 1.82 1.00
256_V 262_V 1.813 1.00
281_R 284_Q 1.811 1.00
47_D 51_E 1.798 1.00
82_N 85_K 1.794 1.00
131_M 143_L 1.77 1.00
151_W 180_A 1.73 0.99
217_V 235_A 1.688 0.99
2_Q 291_I 1.675 0.99
230_R 233_E 1.664 0.99
251_I 256_V 1.662 0.99
214_A 263_G 1.656 0.99
108_R 112_R 1.645 0.99
222_S 246_D 1.64 0.99
216_V 256_V 1.633 0.99
209_A 213_Q 1.63 0.99
181_L 188_I 1.609 0.99
61_S 83_E 1.601 0.99
106_V 164_M 1.594 0.99
109_A 114_E 1.589 0.99
235_A 242_A 1.584 0.99
106_V 130_T 1.572 0.99
254_E 257_K 1.571 0.99
155_V 158_E 1.562 0.99
50_R 55_I 1.556 0.99
23_E 48_S 1.532 0.98
68_I 90_T 1.499 0.98
3_I 279_V 1.489 0.98
245_I 251_I 1.485 0.98
26_L 54_A 1.476 0.98
107_A 111_R 1.471 0.98
214_A 217_V 1.471 0.98
24_N 27_A 1.461 0.98
48_S 52_R 1.435 0.97
180_A 183_K 1.419 0.97
280_A 283_Q 1.419 0.97
207_V 235_A 1.407 0.97
77_S 128_E 1.395 0.97
109_A 162_S 1.391 0.97
212_E 238_M 1.383 0.96
4_L 261_C 1.359 0.96
44_Y 273_I 1.356 0.96
3_I 264_V 1.355 0.96
223_K 244_L 1.32 0.95
151_W 183_K 1.309 0.95
25_A 92_F 1.303 0.95
160_K 187_K 1.303 0.95
247_D 250_D 1.283 0.94
78_Q 81_R 1.275 0.94
179_D 183_K 1.269 0.93
43_R 47_D 1.267 0.93
110_S 134_Y 1.243 0.93
13_A 98_L 1.23 0.92
220_V 245_I 1.226 0.92
216_V 243_F 1.222 0.92
300_I 303_E 1.222 0.92
249_K 277_N 1.211 0.91
77_S 132_G 1.201 0.91
248_A 281_R 1.186 0.90
169_L 226_S 1.186 0.90
22_V 54_A 1.181 0.90
7_N 292_P 1.18 0.90
78_Q 132_G 1.171 0.89
144_V 150_V 1.167 0.89
266_A 275_V 1.166 0.89
121_H 230_R 1.156 0.88
7_N 209_A 1.155 0.88
223_K 250_D 1.155 0.88
104_M 107_A 1.152 0.88
146_S 149_D 1.144 0.88
20_S 24_N 1.144 0.88
228_S 267_G 1.143 0.88
129_G 132_G 1.137 0.87
294_E 301_V 1.135 0.87
76_V 80_V 1.132 0.87
216_V 255_W 1.131 0.87
22_V 35_V 1.122 0.86
199_A 268_A 1.117 0.86
8_P 206_A 1.114 0.86
221_G 267_G 1.112 0.86
280_A 284_Q 1.112 0.86
215_E 260_K 1.112 0.86
218_L 255_W 1.105 0.85
33_I 54_A 1.105 0.85
45_V 48_S 1.103 0.85
303_E 307_E 1.095 0.84
48_S 273_I 1.094 0.84
217_V 293_L 1.093 0.84
61_S 86_S 1.088 0.84
35_V 38_E 1.087 0.84
218_L 251_I 1.08 0.83
176_D 179_D 1.08 0.83
112_R 160_K 1.074 0.83
146_S 150_V 1.071 0.83
210_L 292_P 1.065 0.82
60_I 84_A 1.064 0.82
78_Q 107_A 1.063 0.82
143_L 149_D 1.061 0.82
247_D 277_N 1.042 0.80
78_Q 82_N 1.041 0.80
39_V 92_F 1.04 0.80
292_P 297_E 1.037 0.80
199_A 204_Q 1.036 0.80
109_A 164_M 1.035 0.80
7_N 39_V 1.033 0.79
60_I 69_L 1.028 0.79
81_R 133_Q 1.027 0.79
35_V 54_A 1.027 0.79
22_V 70_I 1.025 0.79
150_V 177_V 1.022 0.78
252_Q 255_W 1.019 0.78
262_V 287_G 1.018 0.78
57_I 63_V 1.016 0.78
303_E 306_K 1.01 0.77
113_G 156_K 1.009 0.77
179_D 182_R 1.006 0.77
102_V 226_S 1.003 0.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4h4dA20.99681000.044Contact Map0.72
4n7bA10.99051000.046Contact Map0.661
3dnfA20.93671000.095Contact Map0.808
4jenA30.487371.10.962Contact Map0.537
4jemA20.462590.965Contact Map0.544
1dbyA10.338651.70.967Contact Map0.144
1t1jA20.319651.30.967Contact Map0.645
3snrA10.506350.60.967Contact Map0.324
1ep7A20.354449.10.967Contact Map0.281
1nswA40.332348.30.967Contact Map0.251
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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